Abstract:
:Translocation of normally non-pathogenic bacteria across the gut may drive inflammatory responses associated with sepsis and inflammatory bowel disease. Recent evidence suggests translocation may not be purely passive, but occurs via novel transcellular pathways activated in enterocytes by inflammatory and metabolic stress. The specificity of this pathway with respect to different E. coli strains and other bacterial species, and possible molecular determinants of the "translocating" phenotype have been investigated. Translocation of E. coli strains and other bacteria was studied across Caco-2 monolayers exposed to different forms of cellular stress. All bacteria, apart from the pathogen Shigella sonnei, exhibited low levels of translocation in untreated monolayers. However, following enterocyte stress, translocation of E. coli strains C25 and HBTEC-1 was markedly stimulated, accompanied by increased internalisation into enterocytes. C25 and HBTEC-1 were typed to ECOR group A and group D respectively. Pathoarray analysis showed both strains had profiles quite different to those predicted for typical ExPEC isolates, lacking many of the genes associated with pathogenicity, although they contained several ORFs in common with ExPEC isolates. These data suggest translocating E. coli strains associated with infections are not opportunistic ExPEC strains but may comprise a separate group of E. coli strains.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Macutkiewicz C,Carlson G,Clark E,Dobrindt U,Roberts I,Warhurst Gdoi
10.1016/j.micinf.2008.01.001subject
Has Abstractpub_date
2008-04-01 00:00:00pages
424-31issue
4eissn
1286-4579issn
1769-714Xpii
S1286-4579(08)00010-5journal_volume
10pub_type
杂志文章abstract::Foot-and-mouth disease virus (FMDV) is a member of the Aphthovirus genus in the Picornaviridae family. Seven distinct serotypes, each including a wide range of variants, have been defined. FMD, affects wild and domesticated ruminants and pigs, is difficult to control and is the major constraint to international trade ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01634-9
更新日期:2002-08-01 00:00:00
abstract::Genomics is accelerating the progress in data generation and interpretation in the global analyses of components of cells, including the spectrum of lipids, RNA, metabolites, proteins, mutational phenotypes or DNA methylation sites. Integration of the knowledge generated by these diverse strategies is predicted to hav...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2005.11.018
更新日期:2006-05-01 00:00:00
abstract::Over the last 10 years - in Microbes and Infection - the publications dealing with protozoan parasites were mainly providing insights on the pathogenic processes leading to the local or systemic damages in the mammals, these parasitic organisms exploit/subvert as hosts. As a result, many investigators introduced the o...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.07.009
更新日期:2008-07-01 00:00:00
abstract::We investigated the thymic and peripheral T-lymphocyte subsets in BALB/c mice undergoing acute or chronic Trypanosoma cruzi infection, in terms of expression of particular Vbeta rearrangements of the T-cell receptor. We first confirmed the severe depletion of CD4(+)CD8(+) thymocytes following acute T. cruzi infection....
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(03)00156-4
更新日期:2003-08-01 00:00:00
abstract::Leprosy is a chronic infection of the skin and nerves caused by Mycobacterium leprae. Erythema nodosum leprosum (ENL) is a reactive state in lepromatous leprosy. Thalidomide has been used to treat ENL since the 1960s. One of its mechanisms of action is anti-inflammatory through selective inhibition of the pro-inflamma...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01645-3
更新日期:2002-09-01 00:00:00
abstract::Protein kinase RNA activated (PKR) is a crucial mediator of anti-viral responses but is reported to be activated by multiple non-viral stimuli. However, mechanisms underlying PKR activation, particularly in response to bacterial infection, remain poorly understood. We have investigated mechanisms of PKR activation in ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.03.010
更新日期:2016-07-01 00:00:00
abstract::The physiopathology of Chagas' disease has been largely defined in murine infections with virulent strains which partially represent parasite diversity. This report reviews our studies with Sylvio X10/4 parasites, a Trypanosoma cruzi clone that induces no acute phase but in C3H/He mice leads to chronic myocarditis res...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2009.07.011
更新日期:2009-11-01 00:00:00
abstract::Neisseria meningitidis is an opportunistic human pathogen that usually colonizes the nasopharyngeal mucosa asymptomatically. Upon invasion into the blood and central nervous system, this bacterium triggers a fulminant inflammatory reaction with the manifestations of septicemia and meningitis, causing high morbidity an...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2015.09.025
更新日期:2016-02-01 00:00:00
abstract::In this study, the effect of Yersinia derivatives on nitric oxide (NO), hydrogen peroxide (H2O2) and tumor necrosis factor-alpha (TNF-alpha) production by murine peritoneal macrophages was investigated. Addition of lipopolysaccharide (LPS) to the macrophage culture resulted in NO production that was dose dependent. On...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2003.10.012
更新日期:2004-02-01 00:00:00
abstract::The Mycobacterium abscessus complex can cause fatal pulmonary disease, especially in cystic fibrosis patients. Diagnosing M. abscessus complex pulmonary disease is challenging. Immunologic assays specific for M. abscessus are not available. In this study seven clinical M. abscessus complex strains and the M. abscessus...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2018.10.006
更新日期:2019-01-01 00:00:00
abstract::Two candidate DNA vaccines based on the proteins CFP10 and CFP21 encoded by regions of difference (RDs) of Mycobacterium tuberculosis were evaluated individually and in multivalent combination with the immunodominant protein Ag85B for induction of protective immune responses against experimental tuberculosis. Experime...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.04.025
更新日期:2006-08-01 00:00:00
abstract::Hepatitis C virus (HCV) causes chronic hepatitis, liver cirrhosis and hepatocellular carcinoma in addition to acute hepatitis. The HCV genome encodes two envelope glycoproteins, E1 and E2. To investigate the role of E1 and E2 in HCV infection, we used a recombinant vesicular stomatitis virus (VSV), VSVdeltaG*, harbori...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2004.09.006
更新日期:2005-01-01 00:00:00
abstract::Burkholderia pseudomallei is a major cause of bacterial septicemias in many parts of the world, particularly Thailand; the known geographic range of the organism appears to be enlarging as awareness of the organism and the disease it causes--melioidosis--increases. B. pseudomallei is intrinsically resistant to most an...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(99)80007-0
更新日期:1999-02-01 00:00:00
abstract::We previously found that, in a mouse model of Chagas cardiomyopathy, 18% of the 9390 quantified unigenes were significantly regulated by Trypanosoma cruzi infection. However, treatment with bone marrow-derived mononuclear cells (MNCs) resulted in 84% transcriptomic recovery. We have applied new algorithms to reanalyze...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2017.11.003
更新日期:2018-03-01 00:00:00
abstract::Afipia felis is a Gram-negative alpha-proteobacterium, a rare cause of human cat scratch disease (CSD), and likely a pathogen of amoeba. Here, we show that various members of the genus Afipia attach to and are taken up by various non-professional phagocytic mammalian cells (epithelial CHO, endothelial EA.hy926, epithe...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.12.007
更新日期:2007-03-01 00:00:00
abstract::Surfactant protein C (SP-C) is a highly hydrophobic protein found in pulmonary surfactant. SP-C is synthesized exclusively in alveolar type II cells as a 21 kDa integral membrane precursor protein and subsequently proteolytically processed to a 3.7 kDa secretory protein. SP-C enhances the adsorption and spreading of p...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2006.04.009
更新日期:2006-07-01 00:00:00
abstract::Oropouche virus is the aetiological agent of Oropouche fever, a zoonotic disease mainly transmitted by midges of the species Culicoides paraensis. Although the virus was discovered in 1955, more attention has been given recently to both the virus and the disease due to outbreaks of Oropouche fever in different areas o...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2017.11.013
更新日期:2018-03-01 00:00:00
abstract::Comparative genomics and microarrays reveal that the genomes of different Salmonella enterica serovars are distinguished from each other by the presence or absence of hundreds of genes. The distribution of these variable genome regions is often not clonal. Therefore, lateral gene transfer (LGT) plays an important role...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(03)00186-2
更新日期:2003-09-01 00:00:00
abstract::This study used a murine model of type 2 diabetes (BKS.Cg-Dock7(m) +/+Lepr(db)/J mice) to investigate the inflammatory and cellular mechanisms predisposing to Burkholderia pseudomallei infection and co-morbid diabetes. Homozygous db/db (diabetic) mice developed extreme obesity, dyslipidaemia and glucose intolerance le...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2011.07.008
更新日期:2011-12-01 00:00:00
abstract::Apoptosis signal-regulating kinase 1 (ASK1) is a mitogen-activated protein kinase kinase kinase which plays pivotal roles in stress and immune responses. This review is focused on three major subjects on ASK1: the regulatory mechanisms of the kinase activity, the pathophysiological roles in stress response and innate ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2005.12.001
更新日期:2006-04-01 00:00:00
abstract::Brucella abortus is an intracellular bacterium that establishes lifelong infections in livestock and humans although the mechanisms of its chronicity are poorly understood. Activated B cells have long lifespan and B. abortus infection activates B cells. Our results indicate that the direct infection of B cells with B....
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.04.001
更新日期:2016-09-01 00:00:00
abstract::Anaerobic bacteria can cause a variety of endogenous infections in children. Because of their fastidious nature, they are difficult to isolate from infectious sites, and are often overlooked. Anaerobic infections can occur in all body sites, including the central nervous system, oral cavity, head and neck, chest, abdo...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01656-8
更新日期:2002-10-01 00:00:00
abstract::Staphylococcus aureus infections are of major importance in human and veterinary medicine. Studies of the virulence of this bacterium are complicated by inconsistent results obtained in different animal models. We searched for an uncomplicated and inexpensive model suitable to study virulence of poultry strains of S. ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.09.006
更新日期:2012-11-01 00:00:00
abstract::Conventional cell lines are commonly used to study infection characteristics of the human gastric pathogen Helicobacter pylori. We sought to investigate bacterial attachment to human antral primary epithelial cells, a cell model that more closely resembles the human stomach than transformed cell lines. Primary cells w...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(00)01322-8
更新日期:2000-11-01 00:00:00
abstract::Numerous Chlamydia trachomatis proteins have been identified as potential subunit vaccines, of which the major outer-membrane protein (MOMP) has, so far, proven the most efficacious. Recently, subunit A of the V-type ATP synthase (ATPase; TC0582) complex was shown to elicit partial protection against infection. Comput...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2015.12.002
更新日期:2016-04-01 00:00:00
abstract::Protective immunity against cutaneous leishmaniasis is dependent on the induction of Th1/Tc1 immune responses resulting in efficient parasite elimination. In this review, the mechanisms leading to protection are discussed with special focus on the role of Leishmania major-infected dendritic cells (DC) in induction of ...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2004.05.024
更新日期:2004-10-01 00:00:00
abstract::Our previous study has demonstrated that IL-10 may modulate both indoleamine 2,3-dioxygenase (IDO) and CD163 expression in lepromatous leprosy (LL) cells, favoring Mycobacterium leprae persistence through induction of regulatory pathways and iron storage. Here, we observed that in LL lesion cells there is an increase ...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2017.06.006
更新日期:2017-11-01 00:00:00
abstract::Myxoma virus (MYXV) is a poxvirus with a strict rabbit-specific host-tropism for pathogenesis. The immunoregulatory factors encoded by MYXV can suppress some functions of immune effectors from other species. We review their mechanisms of action, implications in therapeutics and the potential to improve MYXV as an onco...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2010.08.012
更新日期:2010-12-01 00:00:00
abstract::Phocid herpesvirus virus type 2 (PhHV-2), a putative gammaherpesvirus of seals, has been isolated from harbor seals (Phoca vitulina) and grey seals (Halichoerus grypus). In the present study, different PhHV-2 isolates were shown to have a broad in vitro tropism for various cell types from several mammalian species. In...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.10.006
更新日期:2007-01-01 00:00:00
abstract::Dengue fever and dengue hemorrhagic fever are important diseases worldwide. Although antibody-dependent enhancement of infection has been proposed as a mechanism for increased disease severity, enhancing antibodies in endemic people have not been thoroughly investigated. Recently, we established a serological assay sy...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.07.013
更新日期:2012-11-01 00:00:00