Abstract：
:The suprachiasmatic nucleus of the hypothalamus receives dense serotonergic projections from the raphe nuclei. This input may be involved in the transmission of photic information with activation of 5-HT receptors at night having a photo-mimetic effect. 5-HT2C receptors have been implicated in this role, with 5-HT2C receptor agonist administration at night resulting in the induction of cFOS protein, and a phase delay of melatonin metabolite excretion rhythms in a manner similar to light. To investigate the site of action of 5-HT2C receptor agonists, rat SCN explants were isolated in culture, allowing exposure of agonists to denervated tissue. Any response could then be attributed to the actions of the 5-HT2C receptor agonist on cells within the SCN, rather than at other distant sites or on pre-synaptic receptors. Rat SCN tissue was microdissected and prepared in tissue culture conditions. The acute effect of the 5-HT2A/2C receptor agonist DOI and glutamate administration at various circadian times on c-fos and Per1 expressions was assessed. Glutamate induced the expression of c-fos at CT6, CT16 and CT22, but induced Per1 expression at CT16 only. DOI altered c-fos expression at all times examined with an increase at CT6 and CT22, and a repression at CT16. However, DOI significantly increased Per1 expression when applied at CT16, but had no effect at either CT22 or CT6. This response was similar to that previously observed in vivo. This provides support for the hypothesis that the actions of 5-HT2C receptor agonists are on cells located within the SCN.
journal_name
Brain Resjournal_title
Brain researchauthors
Varcoe TJ,Kennaway DJdoi
10.1016/j.brainres.2008.02.091subject
Has Abstractpub_date
2008-05-13 00:00:00pages
19-28eissn
0006-8993issn
1872-6240pii
S0006-8993(08)00528-3journal_volume
1209pub_type
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