Abstract:
:It has been demonstrated that adipose-derived stem cells (ADSCs) secrete cytokines and exhibit diverse pharmacological actions. The present study examined the unknown pharmacological action of ADSCs regarding whitening effects. A conditioned medium of ADSCs (ADSC-CM) was harvested and the whitening effect of ADSC-CM was studied in melanoma B16 cells. ADSC-CM treatment inhibited the synthesis of melanin and the activity of tyrosinase in a dose dependent manner. To clarify the underlying mechanisms of the whitening action of ADSCs, protein levels of melanogenic proteins were measured by Western blot. Although expressions of microphthalmia-associated transcription factor and tyrosinase-related protein 2 (TRP2) remained unchanged, those of tyrosinase and TRP1 were down-regulated. Transforming growth factor-beta1 (TGF-beta 1), a potent regulator of melanogenic proteins, was neutralized by the addition of a blocking antibody to ADSC-CM, and down-regulated expression of tyrosinase and TRP1 was almost reversed. Collectively, these results indicate that secretary factors of ADSC inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1, which are mainly mediated by TGF-beta1.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Kim WS,Park SH,Ahn SJ,Kim HK,Park JS,Lee GY,Kim KJ,Whang KK,Kang SH,Park BS,Sung JHdoi
10.1248/bpb.31.606subject
Has Abstractpub_date
2008-04-01 00:00:00pages
606-10issue
4eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/31.606journal_volume
31pub_type
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