Assessing vaccine potency using TCRmimic antibodies.

Abstract:

:Dendritic cells (DCs) are highly specialized antigen-presenting cells of the immune system that are efficient at presenting peptide-antigen for the activation of T cells and are often the cell type of choice for vaccine targeting by virtue of high expression levels of MHC and costimulatory molecules. Since the level of peptide-MHC complex significantly influences stimulation of T cells, a proof-of-concept potency assay was developed to directly examine the presentation and density of MHC class I peptides derived from the processing of a model tumor antigen, (hCGbeta), on the surface of DCs. In this study we first generated antibodies (TCR mimics or TCRm) to two peptide-HLA-A*0201 epitopes derived from hCGbeta designated as TMT (40-48) and GVL (47-55). Characterization of each TCRm by ELISA and flow cytometric analysis, demonstrated specific binding to soluble recombinant HLA-A2 protein and HLA-A2.1+ T2 cells loaded with relevant peptide. TCRm reactive against the TMT and GVL epitopes blocked granzyme-B production by peptide-specific cytotoxic T lymphocytes (CTL) lines further supporting their recognition specificity. For the assessment of antigen presentation function, human immature monocyte-derived DCs (iDCs) were treated with the mannose receptor targeting vaccine, B11-hCGbeta and matured with Poly I:C. The TMT and GVL epitope reactive CTL lines responded to vaccine-treated but not vehicle-treated mature DCs (mDCs) with TMT and GVL TCRm specifically blocking IFN-gamma production. The TCRm were then used to directly confirm specific peptide-MHC complexes on mDCs. TCRm staining of vaccine-treated mDCs showed detection of the TMT and GVL peptide-HLA-A2 complexes. These findings demonstrate that TCRms may be important tools for determining the potency of DC-based vaccines.

journal_name

Vaccine

journal_title

Vaccine

authors

Neethling FA,Ramakrishna V,Keler T,Buchli R,Woodburn T,Weidanz JA

doi

10.1016/j.vaccine.2008.02.025

subject

Has Abstract

pub_date

2008-06-13 00:00:00

pages

3092-102

issue

25

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(08)00154-0

journal_volume

26

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Points to consider in the development of a surrogate for efficacy of novel Japanese encephalitis virus vaccines.

    abstract::Although an effective killed virus vaccine to prevent illness due to Japanese encephalitis virus (JEV) infection exists, many authorities recognize that a safe, effective live JEV vaccine is desirable in order to reduce the cost and the number of doses of vaccine required per immunization. A large-scale clinical effic...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/s0264-410x(00)00038-4

    authors: Markoff L

    更新日期:2000-05-26 00:00:00

  • Long-term immunogenicity after single and booster dose of a live attenuated hepatitis A vaccine: results from 8-year follow-up.

    abstract::Live, attenuated hepatitis A vaccines are used widely in China but there is uncertainty regarding the persistence of vaccine-induced anti-HAV antibodies after single dose and booster dose administrated at month 12. A large scale clinical trial to evaluate the live, attenuated hepatitis A vaccine was conducted in Hebei...

    journal_title:Vaccine

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.vaccine.2006.08.004

    authors: Wang XY,Xu ZY,Ma JC,von Seidlein L,Zhang Y,Hao ZY,Han OP,Zhang YL,Tian MY,Ouyang PY,Zhang ZY,Han CQ,Xing ZC,Chen JC

    更新日期:2007-01-05 00:00:00

  • Dendritic cells stimulated with outer membrane protein A (OmpA) of Salmonella typhimurium generate effective anti-tumor immunity.

    abstract::Gram-negative bacterial outer membrane proteins (Omps) have an important role in pathogenesis and signal reception. We previously reported that Acinetobacter OmpA (AbOmpA) induced maturation of bone marrow-derived dendritic cells (BMDCs) and that AbOmpA-primed DCs produced IL-12 which generated Th1 CD4(+) T-cells. We ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2011.01.036

    authors: Jang MJ,Kim JE,Chung YH,Lee WB,Shin YK,Lee JS,Kim D,Park YM

    更新日期:2011-03-16 00:00:00

  • Immunoresponse to hepatitis B vaccination and adherence campaign among injecting drug users.

    abstract::Fifty injecting drug users (IDUs) were recruited to evaluate the efficacy of an hepatitis B virus (HBV) vaccination campaign to be carried out in a Public centre for Drug Users (PCDU). The vaccination schedule was successfully performed in most patients, who showed a great interest in this problem. After three doses o...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(96)00290-3

    authors: Lugoboni F,Migliozzi S,Schiesari F,Pauletto N,Bovo GL,Ciaffoni S,Mezzelani P

    更新日期:1997-06-01 00:00:00

  • Novel antibody-independent receptor-binding SPR-based assay for rapid measurement of influenza vaccine potency.

    abstract::A WHO workshop organized following the 2009 H1N1 pandemic recommended development of alternative influenza vaccine potency assays as high priority that could expedite the release of vaccine lots in the face of future influenza pandemics. We have developed an antibody independent, simple, high throughput receptor-bindi...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2014.02.049

    authors: Khurana S,King LR,Manischewitz J,Coyle EM,Golding H

    更新日期:2014-04-17 00:00:00

  • Characterization of West Nile virus live vaccine candidates attenuated by capsid deletion mutations.

    abstract::Protein C deletion mutants of West Nile virus (WNV) were evaluated for their potential use as live virus vaccine candidates in vivo. Double and triple mutants carrying small deletions and second-site point mutations, as well as mutants with large deletions of 36 and 37 amino acid residues were tested in a stringent mo...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2010.06.045

    authors: Schlick P,Kofler RM,Schittl B,Taucher C,Nagy E,Meinke A,Mandl CW

    更新日期:2010-08-16 00:00:00

  • Hepatitis B specific T cell immunity induced by primary vaccination persists independently of the protective serum antibody level.

    abstract::In 2005, in accordance with recommendations made by the European Medicines Agency, the Italian Drug Agency ordered withdrawal of the hexavalent Hexavac(®) vaccine (Sanofi Pasteur MSD) from the market. Concerns had been raised about the low immunogenicity of the hepatitis B virus component of the vaccine, assessed by m...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2012.11.029

    authors: Carollo M,Palazzo R,Bianco M,Pandolfi E,Chionne P,Fedele G,Tozzi AE,Carsetti R,Romanò L,Ausiello CM

    更新日期:2013-01-07 00:00:00

  • Responses of varicella zoster virus (VZV)-specific immunity in seropositive adults after inhalation of inactivated or live attenuated varicella vaccine.

    abstract::To examine boostering of varicella zoster virus (VZV)-specific immunity in seropositive adults after nasal inhalation of heat-inactivated or live attenuated varicella vaccine, we determined specific cellular immunity, IgG antibody in sera and secretory IgA antibody in saliva before and after the inhalation. The mean t...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(02)00408-5

    authors: Terada K,Niizuma T,Ogita S,Kataoka N

    更新日期:2002-11-01 00:00:00

  • Expanding immunization coverage in rural India: a review of evidence for the role of community health workers.

    abstract::Poor routine immunization coverage in India has led to a large burden of vaccine-preventable diseases borne by children under 5 years of age. Despite efforts to strengthen infrastructure and service delivery in the past decade, immunization coverage rates have reached a plateau. To meet the formidable needs of India's...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2009.10.108

    authors: Patel AR,Nowalk MP

    更新日期:2010-01-08 00:00:00

  • Pandemic H1N1 influenza virus-like particles are immunogenic and provide protective immunity to pigs.

    abstract::The outbreak of the 2009 influenza pandemic underscored the important role of swine in influenza virus evolution and the emergence of novel viruses with pandemic potential. Vaccination is the most common practice to control swine influenza in swine industry. Influenza virus-like particle (VLP) vaccines are an alternat...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2011.12.083

    authors: Pyo HM,Masic A,Woldeab N,Embury-Hyatt C,Lin L,Shin YK,Song JY,Babiuk S,Zhou Y

    更新日期:2012-02-08 00:00:00

  • Safety and immunogenicity of fully liquid and lyophilized formulations of an investigational trivalent group B streptococcus vaccine in healthy non-pregnant women: Results from a randomized comparative phase II trial.

    abstract:BACKGROUND:We evaluated the safety and immunogenicity of liquid and lyophilized formulations of an investigational trivalent group B streptococcus (GBS) vaccine in non-pregnant women and assessed the formulations' equivalence in terms of serotype-specific immune response. METHODS:This phase II, randomized, comparative...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2020.02.085

    authors: Beran J,Leroux-Roels G,Van Damme P,de Hoon J,Vandermeulen C,Al-Ibrahim M,Johnson C,Peterson J,Baker S,Seidl C,Dreisbach A,Karsten A,Corsaro B,Henry O,Lattanzi M,Bebia Z

    更新日期:2020-04-03 00:00:00

  • Dendritic cells expressing BTLA induces CD8+ T cell tolerance and attenuates the severity of diabetes.

    abstract::Numerous evidence demonstrate Type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8(+) T cells play an important role in the development of T1D in NOD mice. The novel coinhibitory receptor BTLA may have a regulatory role in maintaining peripheral tolerance, however, its role in autoimmu...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2011.07.125

    authors: Li S,Zhang M,Xiang F,Zhao J,Jiang C,Zhu J

    更新日期:2011-10-13 00:00:00

  • Protection of pigs against Taenia solium cysticercosis using recombinant antigen or in combination with DNA vaccine.

    abstract::In the present study, we investigated the duration of protection afforded to pigs immunized in two different prime-boost regimens: one is homologus priming and boosting with a protein vaccine, and the other is priming with a DNA vaccine and boosting with the protein vaccine. Groups of pigs that received the same vacci...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2004.07.012

    authors: Guo YJ,Sun SH,Zhang Y,Chen ZH,Wang KY,Huang L,Zhang S,Zhang HY,Wang QM,Wu D,Zhu WJ

    更新日期:2004-09-28 00:00:00

  • V-1 Immunitor: oral therapeutic AIDS vaccine with prophylactic potential.

    abstract::V-1 Immunitor (V1) is a therapeutic vaccine comprising pooled HIV antigens formulated into an oral pill. Recent V1 studies demonstrated body weight gain, increase in CD4 and CD8 cells, decrease in viral load, and improved survival of end-stage AIDS patients. The potential of V1 as a prophylactic vaccine has been evalu...

    journal_title:Vaccine

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0264-410x(02)00570-4

    authors: Jirathitikal V,Sooksathan P,Metadilogkul O,Bourinbaiar AS

    更新日期:2003-01-30 00:00:00

  • Evaluation of the stability of measles vaccine out of the cold chain under extended controlled temperature conditions.

    abstract::Measles outbreaks occur periodically in remote and difficult to reach areas in countries such as the Democratic Republic of Congo. The possibility to keep measles vaccines at temperatures outside the cold chain for a limited period prior to administration would be an advantage for organizations such as Médecins Sans F...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2020.02.005

    authors: Juan-Giner A,Alsalhani A,Panunzi I,Lambert V,Van Herp M,Gairola S

    更新日期:2020-03-04 00:00:00

  • In vivo and in vitro evidence that cross-reactive antibodies to C-terminus of hypervariable region 1 do not neutralize heterologous hepatitis C virus.

    abstract::The hypervariable region 1 (HVR1) of hepatitis C virus (HCV) may contain neutralizing epitopes. A chimpanzee in whom cross-reactive anti-HVR1 antibodies had been induced by immunization was challenged with heterologous HCV for clarifying whether cross-reactive anti-HVR1 antibodies can neutralize heterologous HCV. Acut...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(02)00271-2

    authors: Esumi M,Zhou YH,Tanoue T,Tomoguri T,Hayasaka I

    更新日期:2002-08-19 00:00:00

  • Impact of universal vaccination programmes on the epidemiology of hepatitis B: 10 years of experience in Italy.

    abstract::Ten years have elapsed since routine vaccination of infants and of 12-year-old adolescent was implemented in Italy. In this period, evidence has accumulated on the epidemiological impact of universal immunisation. Coverage is on average >90% and is >or=95% in many areas of the country. Incidence of acute hepatitis B, ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/s0264-410x(02)00580-7

    authors: Bonanni P,Pesavento G,Bechini A,Tiscione E,Mannelli F,Benucci C,Nostro AL

    更新日期:2003-01-30 00:00:00

  • The seroepidemiology of Bordetella pertussis in Israel--Estimate of incidence of infection.

    abstract::This study was undertaken to estimate the magnitude of Bordetella pertussis infections in a highly vaccinated population in Israel in order to evaluate the relationship between clinical notification data and serology-based evidence of infection. A cross-sectional survey was conducted on a total of 1982 serum samples f...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2010.02.104

    authors: Rendi-Wagner P,Tobias J,Moerman L,Goren S,Bassal R,Green M,Cohen D

    更新日期:2010-04-26 00:00:00

  • BoLA-DR peptide binding pockets are fundamental for foot-and-mouth disease virus vaccine design in cattle.

    abstract::This large study investigates the associations between bovine major histocompatibility complex DRB3 alleles and their binding pockets with the immune response to a 40-mer peptide derived from foot-and-mouth disease virus (FMDV) VP1. A crossbred (Charolais and Holstein) cattle population (n=197) was immunised with the ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2009.09.131

    authors: Baxter R,Craigmile SC,Haley C,Douglas AJ,Williams JL,Glass EJ

    更新日期:2009-12-10 00:00:00

  • Enhanced humoral and memory B cellular immunity using HPV16/18 L1 VLP vaccine formulated with the MPL/aluminium salt combination (AS04) compared to aluminium salt only.

    abstract::An effective virus-like particle (VLP) based prophylactic vaccine designed to protect against persistent infection with human papillomavirus (HPV) types 16 and 18 and subsequent lesion development will need to induce a strong humoral and cellular immune response capable of providing long-term protection. Our objective...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2006.06.005

    authors: Giannini SL,Hanon E,Moris P,Van Mechelen M,Morel S,Dessy F,Fourneau MA,Colau B,Suzich J,Losonksy G,Martin MT,Dubin G,Wettendorff MA

    更新日期:2006-08-14 00:00:00

  • A tetravalent meningococcal serogroups A, C, W-135, and Y tetanus toxoid conjugate vaccine is immunogenic and well-tolerated when co-administered with Twinrix(®) in subjects aged 11-17 years: an open, randomised, controlled trial.

    abstract::The co-administration of the tetravalent meningococcal conjugate vaccine, MenACWY-TT, with a licensed hepatitis A and B vaccine, HepA/B (Twinrix(®)), was compared to their separate administration in this open, randomised, controlled study. Healthy subjects 11-17 years of age (n=611) were randomised (3:1:1) to receive ...

    journal_title:Vaccine

    pub_type: 杂志文章,随机对照试验

    doi:10.1016/j.vaccine.2011.11.051

    authors: Østergaard L,Silfverdal SA,Berglund J,Flodmark CE,West C,Bianco V,Baine Y,Miller JM

    更新日期:2012-01-17 00:00:00

  • AIDS and HIV vaccines.

    abstract::In spite of extensive prevention programs, the HIV epidemics is still spreading worldwide, in particularly in developing countries where clade C viruses predominate. WHO estimates that there are 16, 000 new cases of HIV infection daily and that 100 M individuals will be infected by the end of the next decade. In spite...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/s0264-410x(99)00236-4

    authors: Klein M

    更新日期:1999-10-01 00:00:00

  • Healthy individuals' immune response to the Bulgarian Crimean-Congo hemorrhagic fever virus vaccine.

    abstract::Crimean-Congo hemorrhagic fever virus (CCHFV) poses a great threat to public health due to its high mortality and transmission rate and wide geographical distribution. There is currently no specific antiviral therapy for CCHF. This study provides the first in-depth analysis of the cellular and humoral immune response ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2012.08.003

    authors: Mousavi-Jazi M,Karlberg H,Papa A,Christova I,Mirazimi A

    更新日期:2012-09-28 00:00:00

  • VennVax, a DNA-prime, peptide-boost multi-T-cell epitope poxvirus vaccine, induces protective immunity against vaccinia infection by T cell response alone.

    abstract::The potential for smallpox to be disseminated in a bioterror attack has prompted development of new, safer smallpox vaccination strategies. We designed and evaluated immunogenicity and efficacy of a T-cell epitope vaccine based on conserved and antigenic vaccinia/variola sequences, identified using bioinformatics and ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2010.10.064

    authors: Moise L,Buller RM,Schriewer J,Lee J,Frey SE,Weiner DB,Martin W,De Groot AS

    更新日期:2011-01-10 00:00:00

  • BCG vaccine may generate cross-reactive T cells against SARS-CoV-2: In silico analyses and a hypothesis.

    abstract::The world is facing the rising emergency of SARS-CoV-2. The outbreak of COVID-19 has caused a global public health and economic crisis.Recent epidemiological studies have shown that a possible association of BCG vaccination program with decreased COVID-19-related risks, suggesting that BCG may provide protection again...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2020.08.045

    authors: Tomita Y,Sato R,Ikeda T,Sakagami T

    更新日期:2020-09-22 00:00:00

  • Impact of pneumococcal conjugate vaccine in children morbidity and mortality in Peru: Time series analyses.

    abstract::Streptococcus pneumoniae is the leading cause of bacterial pneumonia, meningitis and sepsis in children worldwide. Despite available evidence on pneumococcal conjugate vaccine (PCV) impact on pneumonia hospitalizations in children, studies demonstrating PCV impact in morbidity and mortality in middle-income countries ...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2016.07.027

    authors: Suarez V,Michel F,Toscano CM,Bierrenbach AL,Gonzales M,Alencar AP,Ruiz Matus C,Andrus JK,de Oliveira LH

    更新日期:2016-09-07 00:00:00

  • Vaccine administration in children with chronic kidney disease.

    abstract::Pediatric patients with severe chronic kidney disease (CKD) on conservative treatment, on dialysis, and those with renal transplantation are at a higher risk for infectious diseases as the result of impaired immune responses against infectious agents. Infections in these patients can have drastic consequences for dise...

    journal_title:Vaccine

    pub_type: 杂志文章,评审

    doi:10.1016/j.vaccine.2014.09.038

    authors: Esposito S,Mastrolia MV,Prada E,Pietrasanta C,Principi N

    更新日期:2014-11-20 00:00:00

  • Generation of an immune microenvironment as a novel mechanism for myotoxins to potentiate genetic vaccines.

    abstract::We recently reported that administration of low doses of myotoxins at vaccination sites potentiated antigen-specific T-cell immunity induced by genetic cancer vaccines in mice, an effect which was superior to TLR agonists. In the current study, we found unexpectedly that the mechanism of this potent adjuvant effect wa...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2010.09.084

    authors: Qin H,Cha SC,Neelapu SS,Liu C,Wang YH,Wei J,Qin XF,Liu YJ,Kwak LW

    更新日期:2010-11-23 00:00:00

  • The role of the vaccines industry in Mission Grand Convergence.

    abstract::The vast majority of vaccines used throughout the world are supplied by the private sector. It is essential therefore that the industry is closely engaged in future policy developments at a national and international level and is able to respond to the changing needs and priorities that may be required to ensure the s...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2016.10.084

    authors: Almond J,Medaglini D

    更新日期:2017-01-20 00:00:00

  • Evaluation of adenovirus 19a as a novel vector for mucosal vaccination against influenza A viruses.

    abstract::Since preexisting immunity and enhanced infection rates in a clinical trial of an HIV vaccine have raised some concerns on adenovirus (Ad) serotype 5-based vaccines, we evaluated the subgroup D adenovirus serotype Ad19a for its suitability as novel viral vector vaccine against mucosal infections. In BALB/c mice, we co...

    journal_title:Vaccine

    pub_type: 杂志文章

    doi:10.1016/j.vaccine.2018.02.075

    authors: Lapuente D,Ruzsics Z,Thirion C,Tenbusch M

    更新日期:2018-05-03 00:00:00