Regenerating axons from adult chick retinal ganglion cells recognize topographic cues from embryonic central targets.

Abstract:

:We investigated whether regenerating mature axons recapitulate embryonic features essential to successful reconnectivity within the injured nervous system. Strips from embryonic and adult chick retinae were cultured, and outgrowing axons were examined morphometrically and immunohistochemically. In addition, the target-recognition properties of adult neurites were analyzed. Regenerating adult axons elongate on a poly-L-lysine/laminin substratum with a speed about one order of magnitude slower than that of embryonic axons. Morphologically, adult axonal tips differ dramatically from embryonic growth cones in that they possess only filopodial extensions whereas embryonic growth cones possess both lamellipodial and filopodial processes. Both embryonic and adult neurites express the growth-associated protein GAP-43. When cultured on alternating stripes of anterior and posterior embryonic tectal membranes, both adult and embryonic retinal axons distinguish between the two membrane preparations. Our results demonstrate that during axonal regeneration the mature neurons express embryonic properties that are involved in the recognition of tectal positional cues.

journal_name

Vis Neurosci

journal_title

Visual neuroscience

authors

Vanselow J,Müller B,Thanos S

doi

10.1017/s0952523800002558

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

569-76

issue

6

eissn

0952-5238

issn

1469-8714

pii

S0952523800002558

journal_volume

6

pub_type

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