Abstract:
:Research into active immunisation against gonadotrophin releasing hormone (GnRH-I) has gained widespread acceptance as a means of controlling reproduction and behaviour of farm, companion and wild animals. Many studies describe the use of multiple copies of the self-peptide in linear alignment and conjugation with a large carrier protein to increase the immune response to the peptide. However, problems resulting from carrier protein epitope suppression have seen a diversion of interest into the use of genetic materials to elicit an optimum immune response. In this study, a 533-bp long DNA vaccine was constructed in pcDNAV5-HisB coding for 18.871 kDa GnRH-I-T-helper-V5 epitopes fusion protein. COS1 cells transfected with the vaccine construct were found to release fusion protein into culture supernatant. The vaccine construct (100 microg/mice) in saline solution administered into the anterior quadriceps muscle of ICR male and female mice stimulated antigen-specific IgG antibody responses. Testosterone levels in the vaccinated male mice were significantly (p = 0.021) reduced. A significant reduction in uterine implants were noted following mating between immunised males and control females (p = 0.028), as well as between immunised females and control males (p = 0.004). Histological examination of both the male and female gonads in study week 13 showed atrophy of the seminiferous epithelium and suppression of folliculogenesis.
journal_name
Vaccinejournal_title
Vaccineauthors
Khan MA,Ogita K,Ferro VA,Kumasawa K,Tsutsui T,Kimura Tdoi
10.1016/j.vaccine.2007.12.052subject
Has Abstractpub_date
2008-03-04 00:00:00pages
1365-74issue
10eissn
0264-410Xissn
1873-2518pii
S0264-410X(07)01511-3journal_volume
26pub_type
杂志文章相关文献
VACCINE文献大全abstract::Safe RSV vaccine development has challenged the medical community since a formalin-killed RSV vaccine caused disease exacerbation in the 1960s. Disease was replicated using the bovine RSV system in one of two studies. The studies differed in viral protein dose and length of time between vaccination and infection. Dise...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.10.024
更新日期:2004-03-29 00:00:00
abstract::The search for an effective HIV vaccine continues to be a challenge. Several candidates have been tested in preclinical protocols, and a few have been tested in clinical trials without resounding success. It is unclear why vaccine approaches to control HIV infection have been unsuccessful, but opportunities to dissect...
journal_title:Vaccine
pub_type:
doi:10.1016/j.vaccine.2010.10.073
更新日期:2010-12-06 00:00:00
abstract::For the production of a chemically inactivated Parapoxvirus ovis (PPVO), an adherent bovine kidney cell line was cultivated on Cytodex-3 microcarriers in suspension culture. The inactivated and purified virus particles have shown immune modulatory activity in several animal models. PPVO was produced by a biphasic batc...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.01.032
更新日期:2008-03-17 00:00:00
abstract::Two hundred and fifty children born in 1967 and vaccinated with killed polio vaccine in Sweden were followed for 18 years and tested for neutralizing antibodies against polio. All of them had demonstrable antibodies at the age of 18. Sixty-four children were tested in samples collected throughout the years. After a mo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(90)90244-g
更新日期:1990-10-01 00:00:00
abstract::Chikungunya virus, transmitted by mosquitoes to man, causes an acute illness characterized by fever, rash and striking joint symptoms. US Military investigators developed, manufactured at The Salk Institute-Government Services Division (TSI-GSD), and tested the live, attenuated Chikungunya Vaccine TSI-GSD-218. The man...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.08.025
更新日期:2012-10-19 00:00:00
abstract::The objectives of this study were to evaluate the effects of a therapeutic vaccine intervention with a modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine on the dynamics of a heterologous viral infection in a population of pigs, and to determine the clinical and virological response of p...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.03.031
更新日期:2007-05-30 00:00:00
abstract::In an effort to develop a safe and effective vaccine for the prevention of enterotoxigenic Escherichia coli (ETEC) K99 infections, we have developed a surface antigen display system using pgsA (poly-gamma-glutamate synthetase A) as an anchoring matrix. The recombinant fusion proteins comprised of pgsA and fimbriae pro...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.088
更新日期:2010-05-28 00:00:00
abstract:OBJECTIVE:To assess whether recipients and non-recipients of the human papillomavirus (HPV) vaccine subsequently differ in terms of sexual risk taking behaviour. DESIGN:Cross-sectional survey. Sequential analyses constructed from self-reported age at vaccination, age at first intercourse and age at response. SETTING:...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.07.025
更新日期:2014-09-03 00:00:00
abstract::Respiratory syncytial virus (RSV) is a leading viral cause of respiratory morbidity and mortality in infants and young children worldwide. Low and middle income countries (LMICs) account for approximately 99% of the global mortality estimates in this population, with up to 200,000 RSV deaths per year. The vaccine prod...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2017.02.068
更新日期:2019-11-28 00:00:00
abstract::Liposome and cholera toxin (CT) are considered to be effective antigen delivery vehicles and adjuvants for mucosal vaccines. The effect of these antigen delivery systems on adjuvant responses to mucosally administered pneumococcal polysaccharide (Pnup) was investigated in this study. Both mucosal (e.g. oral) and syste...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)00198-a
更新日期:1996-04-01 00:00:00
abstract::In Balb/c mice, the sterile protective immunity induced by immunization with radiation-attenuated Plasmodium yoelii sporozoites is eliminated by in vivo depletion of CD8+ T lymphocytes, suggesting that cytotoxic T lymphocytes (CTL) against malaria antigens expressed on infected hepatocytes are required for mediating t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90436-n
更新日期:1992-01-01 00:00:00
abstract::Actinobacillus pleuropneumoniae is a gram negative pleiomorphic rod that is the causative agent of a severe, highly infectious and often fatal pleuropneumonia in swine. We have previously reported the construction of genetically-defined stable riboflavin auxotrophs by replacement of a portion of the APP riboflavin bio...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/s0264-410x(00)00076-1
更新日期:2000-06-15 00:00:00
abstract::To identify Mycobacterium tuberculosis (Mtb) antigens as candidates for a subunit vaccine against tuberculosis (TB), we have employed a CD4+ T-cell expression screening method. Mtb-specific CD4+ T-cell lines from nine healthy PPD positive donors were stimulated with different antigenic substrates including autologous ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.10.056
更新日期:2009-01-07 00:00:00
abstract::Limited research has examined HPV vaccination in Appalachia, a region with cervical cancer disparities. We analyzed 2008-2010 National Immunization Survey-Teen data for adolescent females ages 13-17 from Appalachia (n=1951) to identify correlates of HPV vaccination and reasons why their parents do not intend to vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.04.068
更新日期:2013-06-28 00:00:00
abstract::Development of salivary antibodies at the age of 4 or 5 years to group A and C meningococcal polysaccharides (MenA/C PS) was studied among Gambian children, who had received MenA/C conjugate or PS vaccine in infancy. There was also a control group of 64 age matched children. IgG, IgA, and secretory Ig concentrations w...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00180-8
更新日期:2000-10-15 00:00:00
abstract::The co-administration of the tetravalent meningococcal conjugate vaccine, MenACWY-TT, with a licensed hepatitis A and B vaccine, HepA/B (Twinrix(®)), was compared to their separate administration in this open, randomised, controlled study. Healthy subjects 11-17 years of age (n=611) were randomised (3:1:1) to receive ...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2011.11.051
更新日期:2012-01-17 00:00:00
abstract::Vaccines utilizing poorly immunogenic subunit antigens are dependent upon adjuvants to drive the appropriate T cell responses. In an effort to determine the ability of several adjuvants to promote cell-mediated immunity (CMI), we assessed delayed-type hypersensitivity (DTH) in mice inoculated with heat-killed Listeria...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(96)00199-5
更新日期:1997-03-01 00:00:00
abstract::Clostridium difficile is a pathogen with increasing severity for which host antibody responses provide protection from disease. DNA vaccination has several advantages compared to traditional vaccine methods, however no study has examined this platform against C. difficile toxins. A synthetic gene was created encoding ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.03.058
更新日期:2009-06-02 00:00:00
abstract::Irradiation of Androctonus australis hector venom using a dose of 2 kGy has successfully abolished toxicity without reducing its antigenic or immunogenic properties. Toxicity of irradiated antigen was abolished until 20 times of LD(50) of native venom. Analysis of physiopathological effects induced by native and irrad...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.09.044
更新日期:2011-11-08 00:00:00
abstract::MAGE-A3 is highly expressed in epithelial ovarian cancer (EOC), making it a promising candidate for immunotherapy. We investigated whether dendritic cells (DCs) transduced with a rAAV-6 capsid mutant vector Y445F could elicit effective MAGE-A3-specific anti-tumor cytotoxic T lymphocyte (CTL) responses in vitro. MAGE-A...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.12.049
更新日期:2014-02-12 00:00:00
abstract:BACKGROUND:Zostavax™ is a live, attenuated varicella-zoster virus vaccine indicated for the prevention of herpes zoster (shingles). An observational post-licensure (Phase IV) study was conducted at Kaiser Permanente Northern California (KPNC), a US managed care organization, to assess the safety of zoster vaccine in pe...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.08.070
更新日期:2012-10-19 00:00:00
abstract::We report that intraperitoneal injection of Chlamydia pneumoniae purified elementary bodies (EBs) in the hamster causes a systemic infection allowing the isolation of viable chlamydiae from several organs for several days post-infection (p.i.). In particular, spleen infection occurred up to Day 7 p.i. in 100% of anima...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.09.035
更新日期:2004-03-12 00:00:00
abstract::Haemagglutinin (HA), a mixture of haemagglutinin and neuraminidase (HA + NA), and matrix (M) protein were isolated from the influenza A virus and covalently coupled to a synthetic polyelectrolyte (P). A single injection into mice of the resultant conjugates (virogates) brought about efficient stimulation of the primar...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(85)90130-6
更新日期:1985-12-01 00:00:00
abstract::The aims of this study were to estimate influenza vaccination coverage for children during the 2006-2007 influenza season in Ontario, Canada, where universal vaccination is available, and to compare the rate among children aged 6-23 months with corresponding rates from other Canadian provinces that specifically target...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.02.017
更新日期:2009-04-14 00:00:00
abstract::Severe acute respiratory syndrome (SARS) caused by a newly identified coronavirus (SARS-CoV) remains a threat to cause epidemics as evidenced by recent sporadic cases in China. In this communication, we evaluated the efficacy and safety of two SARS vaccine candidates based on the recombinant modified vaccinia Ankara (...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.01.033
更新日期:2005-03-18 00:00:00
abstract::The objective of this study was to investigate the prevalence of hepatitis B markers in a representative sample of 2142 subjects in Catalonia, Spain, and to compare it with previous studies. Multiple logistical regression analysis was carried out to determine variables associated with the markers studied. The prevalen...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00028-1
更新日期:2000-05-08 00:00:00
abstract::Transcutaneous immunization (TCI) has become an attractive alternate route of immunization due to increase understanding of the skin immune system and to recent technical innovations in skin patch delivery systems. Basic principles of TCI have been demonstrated in animal and human studies, covering a variety of bacter...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2011.09.113
更新日期:2012-06-19 00:00:00
abstract::In this paper we give guidance for the design and conduct of vaccine trials against Plasmodium vivax malaria. The paper supplements earlier guidelines on the planning of vaccine trials against Plasmodium falciparum malaria [WHO. Guidelines for the evaluation of Plasmodium falciparum vaccines in populations exposed to ...
journal_title:Vaccine
pub_type: 杂志文章,实务指引,评审
doi:10.1016/j.vaccine.2009.07.018
更新日期:2009-09-18 00:00:00
abstract::Haemophilus parasuis (H. parasuis) is a swine pathogen responsible for the Glässer's disease, which has received more attention in the past decade due to the increasing economic losses in the pig industry worldwide. As traditional inactive vaccine of H. parasuis has obvious disadvantage, to identify efficient immunopr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.02.023
更新日期:2015-03-30 00:00:00
abstract::The anti-tumor immunologic effects of plasmid DNA vaccines encoding human carcinoembryonic antigen (CEA) fused to mouse granulocyte/macrophage colony-stimulating factor (GM-CSF) were examined. Immunization of C57BL/6 mice with the CEA-GMCSF fusion plasmids in a three injection, high-dose immunization schedule led to T...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.08.043
更新日期:2005-01-26 00:00:00