当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Metabolism of eicosapentaenoic and docosahexaenoic acids by recombinant human cytochromes P450.

Metabolism of eicosapentaenoic and docosahexaenoic acids by recombinant human cytochromes P450.

Abstract：

:Epoxidation and hydroxylation of arachidonic acid (AA) are both catalyzed by cytochromes P450s (CYPs). The oxidized metabolites are known to be involved in the regulation of vascular tone and renal function. By using a panel of 15 human recombinant CYPs, this study demonstrates that other polyunsaturated long-chain fatty acids (PUFA-LC), especially the omega3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are also epoxidised. The regioselectivity of epoxidation of four PUFA-LC by CYPs was investigated. Among the several CYPs tested, CYP2C9/2C19 and 1A2 were the most efficient in EPA and DHA epoxidations. It ensued that 10muM of these two omega3 fatty acids decreased by more than 80% and 60%, respectively, the formation by CYP2C9 of AA-epoxidised derivatives. These findings suggest that some physiological effects of omega3 fatty acids may be due to a shift in the generation of active epoxidised metabolites of AA through CYP-mediated catalysis.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Fer M,Dréano Y,Lucas D,Corcos L,Salaün JP,Berthou F,Amet Y

doi

10.1016/j.abb.2008.01.002

subject

Has Abstract

pub_date

2008-03-15 00:00:00

pages

116-25

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(08)00004-0

journal_volume

471

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Development of a peptide antibody specific to human glutathione S-transferase alpha 4-4 (hGSTA4-4) reveals preferential localization in human liver mitochondria.

    abstract：:The reactive cellular products generated during the peroxidation of membrane lipids have been implicated as causative agents in a variety of degenerative diseases and aging. In particular, 4-hydroxynon-2-enal (4HNE) is among the most of the produced during lipid peroxidation. In humans and rodent species, the alpha 4 ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2352

    authors： Gardner JL,Gallagher EP

    更新日期：2001-06-01 00:00:00

  • Vasodilatory effect of kaempferol-7-O-α-L-rhamnopyranoside via NO-cGMP-PKG signaling.

    abstract：:Hypertension is one of the major causes of mortality. Though a host of drugs are available for the treatment of hypertension, majority have been linked to adverse side effects, necessitating the need for research into natural compounds with fewer side effects. Kaempferol-7-O-α-L-rhamnopyroside (KR) is a glycosylated f...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.04.001

    authors： Tettey CO,Yang IJ,Shin HM

    更新日期：2019-05-30 00:00:00

  • Vitamin K-dependent carboxylase: effect of detergent concentrations, vitamin K status, and added protein precursors on activity.

    abstract：:Activity of the rat liver microsomal vitamin K-dependent carboxylase has been studied at various concentrations of detergent. The activity which could be solubilized by 0.25% Triton X-100 was low but could be greatly increased if vitamin K-deficient rats were given vitamin K a few minutes before they were killed. At h...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90519-2

    authors： Shah DV,Swanson JC,Suttie JW

    更新日期：1983-04-01 00:00:00

  • Inhibition of interleukin-2/p55 receptor subunit interaction by complementary peptides.

    abstract：:Complementary peptides to interleukin-2 (IL-2) sequences important for receptor binding were tested for their ability to mimic natural receptors and act as inhibitors of the IL-2/p55 receptor subunit interaction. Peptides hydropathically complementary to IL-2 sequences 15-27 and 40-54 were synthesized in a linear and ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1201

    authors： Fassina G,Cassani G,Gnocchi P,Fornasiero MC,Isetta AM

    更新日期：1995-04-01 00:00:00

  • Ferritin mRNA is found on bound as well as on free polyribosomes in rat heart.

    abstract：:Free and endoplasmic reticulum-bound polyribosomes from rat heart were examined for their ferritin mRNA content. A procedure for separation and purification of the two ribosome populations that produced good yields of homogeneous mono- and polyribosomes with no contaminating ultrastructures and gave distinctive sedime...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90165-3

    authors： Campbell CH,Ismail R,Linder MC

    更新日期：1989-08-15 00:00:00

  • The bacteriorhodopsin proton pump: effect of crosslinkings of lysine residues.

    abstract：:All six available lysine residues in bacteriorhodopsin were amidinated with dimethyl-3,3'-dithiobispropionimidate, which is a crosslinking agent. The photocycle was studied by measuring light absorption and electric signals. The data show an essential change in the photocycle: instead of single components, the rise of...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90502-4

    authors： Tóth-Boconádi R,Taneva SG,Kiselev AV,Abdulaev NG,Keszthelyi L

    更新日期：1988-02-01 00:00:00

  • Modification of cysteine residues by N-ethylmaleimide inhibits annexin II tetramer mediated liposome aggregation.

    abstract：:A role of cysteine residues in annexin II tetramer (AIIt)'s function was investigated using the sulfhydryl reagent N-ethylmaleimide (NEM). Incubation of AIIt with NEM resulted in a dose-dependent inhibition of AIIt-mediated liposome aggregation and loss of sulfhydryl groups of AIIt. The concentration effecting 50% inh...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1994

    authors： Singh TK,Liu L

    更新日期：2000-09-15 00:00:00

  • Adaptation of protein carbonyl detection to the requirements of proteome analysis demonstrated for hypoxia/reoxygenation in isolated rat liver mitochondria.

    abstract：:The key technique in proteome analysis is high-resolution two-dimensional (2D) electrophoretic separation of proteins from biological samples. This method combines isoelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Derivatization of protein carbonyls with 2, 4-dinitrop...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1680

    authors： Reinheckel T,Körn S,Möhring S,Augustin W,Halangk W,Schild L

    更新日期：2000-04-01 00:00:00

  • Purification and characterization of glucosamine-6-phosphate deaminase from dog kidney cortex.

    abstract：:Glucosamine-6-phosphate deaminase (EC 5.3.1.10) from dog kidney cortex was purified to homogeneity, as judged by several criteria of purity. The purification procedure was based on two biospecific affinity chromatography steps, one of them using N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose, an immobili...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90664-i

    authors： Lara-Lemus R,Libreros-Minotta CA,Altamirano MM,Calcagno ML

    更新日期：1992-09-01 00:00:00

  • Identification of a topology control domain in the tetracycline resistance protein.

    abstract：:Two N-terminal fusion proteins combining Escherichia coli maltose-binding protein (MBP) and the 12-transmembrane-segment pBR322 tetracycline resistance protein (Tet) have been constructed to determine the strength and location of topology control signals within the N-terminal portion of the Tet protein. The fusions co...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1487

    authors： Miller KW,Jewell JE

    更新日期：1995-10-01 00:00:00

  • Cytochrome P450 IIIA1 (P450p) requires cytochrome b5 and phospholipid with unsaturated fatty acids.

    abstract：:In contrast to other P450 enzymes purified from rat liver microsomes, purified P450 IIIA1 (P450p) is catalytically inactive when reconstituted with NADPH-cytochrome P450 reductase and the synthetic lipid, dilauroylphosphatidylcholine. However, purified P450 IIIA1 catalyzes the oxidation of testosterone when reconstitu...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90128-6

    authors： Eberhart DC,Parkinson A

    更新日期：1991-12-01 00:00:00

  • Subunit interactions of Escherichia coli F1-ATPase: mutants of the gamma subunits defective in interaction with the epsilon subunit isolated by the yeast two-hybrid system.

    abstract：:Previously, we established a method to detect subunit interactions of F1-ATPase by the yeast two-hybrid system (Moritani, C., et al. Biochim. Biophys. Acta 1274, 67-72, 1996). Here, we isolated mutants of the gamma subunits defective in interaction with the epsilon subunit by this new procedure to study the molecular ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0397

    authors： Sawada K,Watanabe H,Moritani-Otsuka C,Kanazawa H

    更新日期：1997-12-01 00:00:00

  • C-terminal Ser/Pro-Thr-Glu-Leu tetrapeptides of prostaglandin endoperoxide H synthases-1 and -2 target the enzymes to the endoplasmic reticulum.

    abstract：:Prostaglandin endoperoxide H synthases-1 and -2 (PGHS-1 and -2) are integral membrane proteins associated with the luminal surface of the endoplasmic reticulum (ER) and the nuclear envelope (NE). The C-terminal sequences of PGHSs end in -Ser/Pro-Thr-Glu-Leu (-S/PTEL), a sequence similar to one of the known ER targetin...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0430

    authors： Song I,Smith WL

    更新日期：1996-10-01 00:00:00

  • The membrane-interactive tail of cytochrome b(5) can function as a stop-transfer sequence in concert with a signal sequence to give inversion of protein topology in the endoplasmic reticulum.

    abstract：:Sequence analyses of the C-terminal membrane intercalative region of the rat cytochrome b(5) indicated that this domain has, in addition to a signal sequence, a combined element of the classic stop-transfer sequence typically found in a variety of transmembrane proteins. Such bitopic protein arrangements arise by tand...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(03)00051-1

    authors： Kaderbhai MA,Morgan R,Kaderbhai NN

    更新日期：2003-04-15 00:00:00

  • Kinetic analysis of the binding of Ulex europeas agglutinin 1 (UEA 1) to H-antigenic fucolipid.

    abstract：:The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several orde...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1594

    authors： Thomas CJ,Surolia A

    更新日期：2000-02-01 00:00:00

  • Presence of an essential lysine residue in a GDP-fucose protected site of the alpha 1----3fucosyltransferase from human small cell lung carcinoma NCl-H69 cells.

    abstract：:The NCI-H69 cell alpha 1----3fucosyltransferase has been purified from a 0.2% Triton X-100R solubilized enzyme fraction by GDP-hexanolamine-Sepharose affinity chromatography and Superose 12 gel filtration. Photoaffinity labeling experiments with 125I-GDP-hexanolaminyl-4-azidosalicylic acid present in concentrations eq...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90611-y

    authors： Holmes EH

    更新日期：1992-08-01 00:00:00

  • Transmembrane domain interactions affect the stability of the extracellular domain of the human NTPDase 2.

    abstract：:Human NTPDase2 and chicken NTPDase8 are cell surface nucleotidases that contain two transmembrane domains (TMD) and five apyrase conserved regions (ACRs). ACR1 is located near the N-terminal TMD whereas ACR5 is located near the C-terminal TMD. The human NTPDase2 activity is decreased by low concentration of NP-40 and ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.02.011

    authors： Chiang WC,Knowles AF

    更新日期：2008-04-15 00:00:00

  • Enhancement of sphingosine kinase 1 catalytic activity by deletion of 21 amino acids from the COOH-terminus.

    abstract：:Sphingosine kinase 1 (SphK1) responds to a variety of growth factor signals by increasing catalytic activity as it translocates to the plasma membrane (PM). Several studies have identified amino acids residues involved in translocation yet how SphK1 increases its catalytic activity remains to be elucidated. Herein, we...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2009.11.006

    authors： Hengst JA,Guilford JM,Conroy EJ,Wang X,Yun JK

    更新日期：2010-02-01 00:00:00

  • Expression of enzymatically active human granzyme 3 in Escherichia coli for analysis of its substrate specificity.

    abstract：:Human granzyme 3 (Gr3) is a serine protease contained in the granules of natural killer cells and cytotoxic T lymphocytes. To elucidate the biochemical and physiological characteristics of Gr3, we attempted to prepare an enzymatically active recombinant human Gr3 without refolding and proteolytic activation. An expres...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.12.001

    authors： Hirata Y,Inagaki H,Shimizu T,Li Q,Nagahara N,Minami M,Kawada T

    更新日期：2006-02-01 00:00:00

  • Antioxidant defenses in human blood plasma and extra-cellular fluids.

    abstract：:I had the fortune to be introduced to Helmut Sies during the mid 1980s, while working as a post-doctoral scientist at the University of California, Berkeley. At that time, Helmut was a frequent visitor of the Bruce Ames' laboratory and a leading authority in antioxidants and oxidative stress. His concepts, ideas and w...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2015.11.021

    authors： Stocker R

    更新日期：2016-04-01 00:00:00

  • Functional communication between PKC-targeted cardiac troponin I phosphorylation sites.

    abstract：:Increased protein kinase C (PKC) activity is associated with heart failure, and can target multiple cardiac troponin I (cTnI) residues in myocytes, including S23/24, S43/45 and T144. In earlier studies, cTnI-S43D and/or -S45D augmented S23/24 and T144 phosphorylation, which suggested there is communication between clu...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2017.05.019

    authors： Lang SE,Stevenson TK,Schatz TM,Biesiadecki BJ,Westfall MV

    更新日期：2017-08-01 00:00:00

  • Interrelation between salvage of purine nucleotides and protein synthesis in rat heart cells.

    abstract：:The effect of transition from a respiring to a respiration-inhibited state on the rate of protein synthesis was investigated in glycolyzing, cultured rat heart cells. The rate was found to be significantly lower after blocking respiration, and it was further decreased by L-lactate. In contrast, pyruvate or phenazine m...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90615-0

    authors： Ravid K,Diamant P,Avi-Dor Y

    更新日期：1985-01-01 00:00:00

  • Modulation of the activity of NAD malic enzyme from solanum tuberosum by changes in oligomeric state.

    abstract：:The effects of pH, NaCl, and malate2- on the equilibrium between dimeric and higher-molecular-weight forms of NAD malic enzyme from Solanum tuberosum var. Chieftain have been analyzed by monitoring the kinetic changes associated with disaggregation [S. D. Grover and R. T. Wedding (1982) Plant Physiol. 70, 1169-1172]. ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90288-1

    authors： Grover SD,Wedding RT

    更新日期：1984-11-01 00:00:00

  • Metabolic impact of overexpression of liver glycogen synthase with serine-to-alanine substitutions in rat primary hepatocytes.

    abstract：:To investigate the effect of elevation of liver glycogen synthase (GYS2) activity on glucose and glycogen metabolism, we performed adenoviral overexpression of the mutant GYS2 with six serine-to-alanine substitutions in rat primary hepatocytes. Cell-free assays demonstrated that the serine-to-alanine substitutions cau...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.08.006

    authors： Kadotani A,Fujimura M,Nakamura T,Ohyama S,Harada N,Maruki H,Tamai Y,Kanatani A,Eiki J,Nagata Y

    更新日期：2007-10-15 00:00:00

  • Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium.

    abstract：:Cellobiose dehydrogenase (CDH), an extracellular hemoflavoenzyme produced by the cellulose-degrading cultures of Phanerochaete chrysosporium, oxidizes cellobiose to cellobionolactone. CDH has been purified to homogeneity by a five-step purification procedure. The homogeneous CDH is monomeric and has a relative molecul...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1098

    authors： Bao W,Usha SN,Renganathan V

    更新日期：1993-02-01 00:00:00

  • Thermodynamic, enzymatic and structural effects of removing a salt bridge at the base of loop 4 in (pro)caspase-3.

    abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.01.011

    authors： Walters J,Swartz P,Mattos C,Clark AC

    更新日期：2011-04-01 00:00:00

  • On the mechanism of action of free purine bases on DNA synthesis in serum-starved L-cells treated with platelet extract.

    abstract：:It has previously been found that there is a synergistic effect of free purine bases and low concentrations of dialyzed platelet extract on net synthesis of DNA in serum-starved fibroblast-like mouse L-cells. Experiments with a mutant line of L-cells that was deficient in hypoxanthine phosphoribosyl transferase (EC 2....

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90699-x

    authors： Flodgaard H,Klenow H

    更新日期：1985-06-01 00:00:00

  • Comparative activity of rat liver dihydrofolate reductase with 7,8-dihydrofolate and other 7,8-dihydropteridines.

    abstract：:The various interactions of rat liver dihydrofolate reductase with two unconjugated 7,8-dihydropteridines, 7,8-dihydrobiopterin and 6-methyl-7,8-dihydropteridine, have been compared with those of 7,8-dihydrofolate and folate. Of particular interest was the reactivity demonstrated by 7,8-dihydrobiopterin because of the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90674-5

    authors： Webber S,Whiteley JM

    更新日期：1985-02-01 00:00:00

  • Role of Ca2+-independent phospholipase A2 on arachidonic acid release induced by reactive oxygen species.

    abstract：:Previous studies have shown that reactive oxygen species (ROS) enhance arachidonic acid (AA) release and the subsequent AA metabolism in macrophages. The purpose of this study was determined the implication of phospholipases A2 (PLA2s) in these events. Our results show that oxidative stress induced by exogenous adding...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2439

    authors： Martínez J,Moreno JJ

    更新日期：2001-08-15 00:00:00

  • Properties of the enzymes catalyzing the biosynthesis of lysophosphatidate and its ether analog in cultured fibroblasts from Zellweger syndrome patients and normal controls.

    abstract：:The activities, properties, and steady-state kinetics of the five enzymes catalyzing the synthesis of 1-acyl- and 1-alkyl-sn-glycerol 3-phosphate in the cultured skin fibroblasts from Zellweger syndrome patients and normal controls were studied in detail. Judging from their Km and Vmax values, glycerol phosphate acylt...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90144-5

    authors： Webber KO,Datta NS,Hajra AK

    更新日期：1987-05-01 00:00:00