Abstract:
:Treatment of Xenopus blastula with activin converts undifferentiated presumptive ectoderm (animal cap) into mesoderm and endoderm in a dose-dependent manner. At low concentrations, activin induces ventral mesoderm such as blood cells. Here we show that activin-treated aggregates of animal cap cells prepared from undifferentiated presumptive ectoderm and transplanted into Xenopus embryos differentiated to form red blood cells and vascular endothelial cells. We compared gene expression profiles of the activin-treated with untreated aggregates of animal cap cells using microarray analysis. This revealed 838 clones including vascular-related genes that were expressed at levels at least 2-fold greater in the activin-treated aggregates than in the untreated controls. Of these, 356 were known Xenopus genes, 296 had homologues, and 186 were unknown genes. These findings identified novel vascular-related genes and provided insights into how the blood vessel system establishes in normal development.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Nagamine K,Furue M,Fukui A,Matsuda A,Hori T,Asashima Mdoi
10.1248/bpb.30.1856subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1856-9issue
10eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/30.1856journal_volume
30pub_type
杂志文章abstract::The purpose of this study was to evaluate the accuracy of the recommended theophylline therapeutic range in the treatment of acute airway obstruction. Twenty seven patients (20 to 64 years) with acute asthma attack were given aminophylline intravenously to obtain a theophylline concentration between 10 and 20 microgra...
journal_title:Biological & pharmaceutical bulletin
pub_type: 临床试验,杂志文章
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journal_title:Biological & pharmaceutical bulletin
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journal_title:Biological & pharmaceutical bulletin
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