Abstract:
:Theiler's murine encephalomyelitis virus (TMEV) infects macrophages and causes demyelinating disease (DD) in certain mouse strains. IL-23 p19/p40 and IFN-beta, which are both expressed by macrophages in response to TMEV, could contribute to or prevent DD. Because TMEV may induce macrophages' cytokines through TLR3 and TLR7 (toll-like receptors), their role in TMEV-induced IL-23 and IFN-beta expression by the RAW264.7 macrophage cell line was determined following infection with TMEV or stimulation with the poly (I:C) or loxoribine. TMEV infection or stimulation with poly (I:C), a TLR3 agonist, or loxoribine, a TLR7 agonist, induced expression of IL-23 and IFN-beta in RAW264.7 cells. In addition, TMEV infection increased expression of TLR3 and TLR7 in RAW264.7 cells. Transfection of RAW264.7 cells with shRNA plasmid vectors expressing siRNA specific for TLR3 or TLR7 concomitantly decreased expression of TLR3 or TLR7, respectively, and TMEV-induced p19 mRNA, p19 protein, and IL-23 p19/p40. Transfection with TLR7-shRNA plasmids reduced expression of TMEV-induced p40 mRNA and p40 protein. However, transfection with TLR3-shRNA plasmids increased expression of TMEV-induced p40 mRNA but decreased p40 protein. In addition, transfection with TLR3-shRNA plasmids but not TLR7-shRNA plasmids decreased expression of TMEV-induced IFN-beta mRNA. Thus TLR3 and TLR7 contribute to TMEV-induced IL-23 p19 and p40, while TLR3 contributes to TMEV-induced IFN-beta.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Al-Salleeh F,Petro TMdoi
10.1016/j.micinf.2007.07.001subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1384-92issue
11eissn
1286-4579issn
1769-714Xpii
S1286-4579(07)00257-2journal_volume
9pub_type
杂志文章abstract::We previously reported prolonged HIV-1 transcriptional gene silencing by an RNA duplex targeting a sequence located within the NF-kappaB binding motif of the HIV-1 promoter in a susceptible HeLa cell line. Here we report extremely prolonged suppression of productive HIV-1 infection in a T-cell line (Molt-4) by a retro...
journal_title:Microbes and infection
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.04.025
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journal_title:Microbes and infection
pub_type: 杂志文章
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journal_title:Microbes and infection
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doi:10.1016/s1286-4579(00)01298-3
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2004.12.001
更新日期:2005-02-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2014.10.010
更新日期:2015-01-01 00:00:00
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2017.12.012
更新日期:2018-04-01 00:00:00
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2016.03.002
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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