Abstract:
:Colorectal cancer is the third most common cause of cancer-related deaths in the world. Surgical intervention followed by chemotherapy remains the primary approach to treatment since colon cancers remain refractory to most chemotherapeutic agents. Based on that, we established a program to screen natural products for cytotoxic activity, employing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay system utilizing HT-29 human colon cancer cells. During the course of our screening, we found that the methanolic extract of silkworm droppings (SDME) has cytotoxic effects on HT-29 cells. In the present study, we investigated the possible mechanisms by which SDME exerts its antiproliferative activity in HT-29 cells. As expected, SDME inhibited growth of HT-29 cells in a dose-dependent manner as assessed by the MTT reduction assay, the lactate dehydrogenase release assay, and the colony formation assay. We also investigated whether the apoptotic effects induced by SDME involve the caspase pathway using the caspase colorimetric assay. Interestingly, caspase-9 and -3, but not caspase-8, were activated in response to SDME treatment. Taken together, these results clearly indicate that the induction of apoptosis by SDME involves a mitochondrial-mediated pathway and strongly suggest that SDME may potentially be a chemotherapeutic agent for human colon cancer.
journal_name
J Med Foodjournal_title
Journal of medicinal foodauthors
Hwang JH,Kim JY,Cha MR,Park HRdoi
10.1089/jmf.2006.165subject
Has Abstractpub_date
2007-09-01 00:00:00pages
467-72issue
3eissn
1096-620Xissn
1557-7600journal_volume
10pub_type
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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doi:10.1089/jmf.2009.0018
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journal_title:Journal of medicinal food
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doi:10.1089/jmf.2012.0184
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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doi:10.1089/10966200152744454
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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abstract::We investigated the effect of solid-state fermented rice cultured with Basidiomycota (sangwhang) and Monascus ruber on lipid metabolism and antioxidant activity. Forty 4-week-old male Sprague-Dawley rats were fed high cholesterol diets in which carbohydrate sources in the treatment groups consisted of non-fermented ri...
journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
pub_type: 杂志文章
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
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journal_title:Journal of medicinal food
pub_type: 杂志文章
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abstract::We previously reported hyperproliferation and hyperplasia in C57Bl/6 mouse mammary gland after feeding a Western-style diet (WD); these findings decreased after supplementing WD with increased calcium and vitamin D(3). We now again fortified WD with increased calcium and vitamin D(3) from two sources: (1) a food sourc...
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