Abstract：
:A method to study the protein binding patterns of trace elements in human cerebrospinal fluid (CSF) is described. Proteins in CSF samples were separated by size exclusion chromatography combined with high performance liquid chromatography (SEC-HPLC). The column was calibrated to separate proteins in the molecular weight range 6-70 kDa. Fractions were then analyzed off-line for trace elements using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). We were able to accurately determine more than 10 elements of clinical interest in the CSF fractions. Results are presented for Cd, Mn, Fe, Pb, Cu and Zn. The total concentrations of 16 trace elements in human plasma and CSF are also presented. The method was able to differentiate the relative contribution of metallothionein and other proteins towards metal binding in human CSF.
journal_name
Brain Resjournal_title
Brain researchauthors
Gellein K,Roos PM,Evje L,Vesterberg O,Flaten TP,Nordberg M,Syversen Tdoi
10.1016/j.brainres.2007.08.004subject
Has Abstractpub_date
2007-10-12 00:00:00pages
136-42eissn
0006-8993issn
1872-6240pii
S0006-8993(07)01807-0journal_volume
1174pub_type
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