Abstract:
:This study probed the changes of keratocytes cultured under simulated microgravity. Keratocytes were isolated from rabbit corneas using collagenase digestion method. Cells were seeded in a 55-mL capacity high-aspect-ratio vessel (HARV) of rotary cell culture system (RCCS) at a density of 1 x 10(4) cells/mL. Dehydrated bovine acellular corneal stroma (5 x 5 x 1 mm, n = 30) was used as a carrier for keratocyte culture. Rotational speed was set at 15, 20, and 30 rpm in the first, second, and third week of culture, respectively. Histological evaluation showed that keratocytes in simulated microgravity culture grew into carriers, but those under conventional gravity grew on the surface of carriers. Scanning electron microscopic evaluation showed that after 19 days in culture, keratocytes on the carriers were spherical and spread in the spaces among the collagen fibers. Cells were dendritic or spindle shaped, and they developed many foot processes linked with surrounding cells. The absorbance values of the simulated microgravity group were significantly higher (P < 0.01) than that of the conventional group from 10 to 19 days of culture. The RCCS obviously enhanced the proliferation of rabbit keratocytes and facilitated the cells' growth into or on the dehydrated bovine acellular corneal stroma. Cells showed more natural morphology.
journal_name
Artif Organsjournal_title
Artificial organsauthors
Chen J,Chen R,Gao Sdoi
10.1111/j.1525-1594.2007.00440.xsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
722-31issue
9eissn
0160-564Xissn
1525-1594pii
AOR440journal_volume
31pub_type
杂志文章abstract::In chronic cardiomyopathy, mechanical circulatory support (MCS) plays an increasingly important role for children as the shortage of suitable donor hearts increases waiting time on the transplant list. We report our experience with the paracorporal Berlin Heart EXCOR System (Berlin Heart AG, Berlin, Germany) used as a...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12062
更新日期:2013-08-01 00:00:00
abstract::This study demonstrates in a novel way how volume and shape are restored to denervated degenerated muscles due to a special pattern of electrical stimulation. To this purpose, Spiral Computer Tomography (CT) and special image processing tools were used to develop a method to isolate the rectus femoris from other muscl...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00610.x
更新日期:2008-08-01 00:00:00
abstract::We assessed the relationship of certain clinical variables (including bradykinin [BK] release and dialysis membrane) to initial mean arterial pressure (MAP) reduction in 47 patients requiring continuous renal replacement therapy (CRRT) in an intensive care unit. The pretreatment MAP was 84 +/- 14 mm Hg for the group a...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2001.06703.x
更新日期:2001-12-01 00:00:00
abstract::Renal Solutions Allient Sorbent Hemodialysis System utilizes a two-chambered pneumatic pump (Pulsar Blood Pump, Renal Solutions, Inc., Warrendale, PA, USA) to avoid limitations associated with peristaltic pumping systems. Single-needle access is enabled by counter-pulsing the two pump chambers, thereby obviating compl...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00724.x
更新日期:2009-04-01 00:00:00
abstract::Testing failed to detect free isocyanates in dialyzer rinses or significant levels of immunoglobulin E antibodies to isocyanate-protein conjugates in the serum of individuals reacting during hemodialysis with dialyzers containing Cuprophan membranes. Extended immunization of rabbits with Limulus lysate-reactive materi...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1984.tb04298.x
更新日期:1984-08-01 00:00:00
abstract::Small-caliber decellularized xenografts with surface heparin coating are known to reduce in vivo thrombogenicity. This study was performed to examine whether heparin coating on the small-caliber decellularized xenografts would reduce macrophage infiltration and intimal hyperplasia. In a rabbit model of bilateral carot...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00748.x
更新日期:2009-06-01 00:00:00
abstract::This paper presents an overview of the U.S. Food and Drug Administration's regulatory requirements for introducing medical device and diagnostic products into commercial distribution in the United States. For any specific product, the requirements vary depending upon the product's associated risks, classification, and...
journal_title:Artificial organs
pub_type: 临床试验,杂志文章
doi:10.1111/j.1525-1594.1982.tb04146.x
更新日期:1982-11-01 00:00:00
abstract::In a preliminary experiment, 38% of the spent dialysis fluid water was reclaimed by a forward osmosis process through a cellulose triacetate membrane. The simplicity of forward osmosis and its minimal external energy requirements may allow the construction of a small bulk device that can reclaim a considerable portion...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00816.x
更新日期:2009-12-01 00:00:00
abstract::In a typical open-heart surgery, the blood flow through the aortic cannula is a critical element of the cardiopulmonary bypass (CPB) procedure. Especially for the neonatal and pediatric CPB flow conditions, the need for small hydraulic diameter and large blood flow results confined turbulent jet flow regimes that exac...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13462
更新日期:2019-10-01 00:00:00
abstract::During revision of a failed acetabular component with well-fixed antiquated femoral stem, the same variant of metal head is usually not available in the inventory production. We gathered the stems retrieved from revision surgeries and tried to reassemble with the commercially available femoral heads. This study report...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2010.01015.x
更新日期:2010-08-01 00:00:00
abstract::Polyvinyl chloride (PVC) is one of the most frequently used polymers for the manufacturing of medical devices. Limitations for its usage are based upon unfavorable surface properties of the polymer including its hydrophobicity and lack of functionalities in order to increase its versatility. To address this issue, wet...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2012.01462.x
更新日期:2012-09-01 00:00:00
abstract::Although effects of biochemical modulation of stem cells have been widely investigated, only recent advances have been made in the identification of mechanical conditioning on cell signaling pathways. Experimental investigations quantifying the micromechanical environment of mesenchymal stem cells (MSCs) are challengi...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12547
更新日期:2016-03-01 00:00:00
abstract::Loss of photoreceptor cells is one of the major causes of blindness. Several groups are exploring the functional replacement of photoreceptors by a retinal prosthesis. The goal of this study was to simulate the vision levels that recipients of retinal prostheses with 4 x 4, 6 x 10, and 16 x 16 electrode arrays may exp...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2003.07309.x
更新日期:2003-11-01 00:00:00
abstract::There is increasing evidence showing that adult stem cells are useful for tissue regeneration. Bone marrow mesenchymal stem cells (MSCs) are self-renewing and are potent in differentiating into multiple cells and tissues. To investigate the practicability of repairing burn wounds with tissue-engineered (TE) skin combi...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00654.x
更新日期:2008-12-01 00:00:00
abstract::We compared in vitro rates of hemolysis for a recently developed centrifugal pump with a conventional roller pump (10-10-00; Stöckert, Munich, Germany). Flow rates of 0.3 L/min and 1 L/min and a pressure of 200 mm Hg were chosen to simulate conditions during neonatal extracorporeal membrane oxygenation (ECMO). There w...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1996.tb04485.x
更新日期:1996-05-01 00:00:00
abstract::The ventricular assist device inflow cannulation site is the primary interface between the device and the patient. Connecting these cannulae to either atria or ventricles induces major changes in flow dynamics; however, there are little data available on precise quantification of these changes. The objective of this i...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2010.01093.x
更新日期:2010-09-01 00:00:00
abstract::Recently, the use of electrolyzed solutions has attracted considerable interest in Japan. This study investigates the efficiency of electrolyzed solutions as disinfecting agents (DA) in the reuse of dialyzers and compares their efficiency to that of other disinfectants currently in use. The following 3 methods were em...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2000.06611.x
更新日期:2000-12-01 00:00:00
abstract::We have developed a novel method to simultaneously evaluate thromboresistance of two different types of drip chambers with a filter used in extracorporeal blood circuits for hemodialysis using canine arteriovenous shunt models. Bilateral bypasses between the femoral artery and vein on both sides of the animal were for...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb00354.x
更新日期:1997-02-01 00:00:00
abstract::The optimization of the monomer composition, using a polymerization kinetic study and the mechanical behavior study of the composites, was the principal objective of this work. The monomeric mixture analyzed is a part of the composite that has been in development at the University of Campinas, Brazil. Parameters such ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1046/j.1525-1594.2003.07250.x
更新日期:2003-05-01 00:00:00
abstract::Planar particle image velocimetry was used to explore the flow behavior of the newly designed 12-cc Penn State pneumatic pediatric assist pump. Wall shear maps complemented the velocity data. Bjork-Shiley Monostrut 17-mm mechanical heart valves were used in the inlet and outlet ports. In comparison with larger Penn St...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2008.00565.x
更新日期:2008-06-01 00:00:00
abstract::The flow of blood as a viscous, Newtonian, incompressible liquid in an expanding spherical cavity with a ball-type inlet valve was examined. This type of flow can serve as a model of the phase of ventricular diastole in a sac-type artificial heart. The critical values of Reynolds numbers at which flow interruption beh...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1983.tb04170.x
更新日期:1983-02-01 00:00:00
abstract::An increasing amount of evidence points to cerebral embolization during cardiopulmonary bypass (CPB) as the principal etiologic factor of neurologic complications. In this study, the capability of capturing and classification of gaseous emboli and pressure drop of three different membrane oxygenators (Sorin Apex, Teru...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2009.00955.x
更新日期:2009-11-01 00:00:00
abstract::We have been developing a new type of centrifugal pump for long-term use. The magnetically suspended centrifugal pump (MSCP) contains no shaft and seal so that long life expectancy is predicted. Paracorporeal left ventricular (LV) assist circulation between the left atrium and the descending aorta was instituted using...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1997.tb03713.x
更新日期:1997-07-01 00:00:00
abstract::The effects of Fluosol-DA 20% on circulating blood cells, hemodynamic parameters, blood gases, and organs are described and compared with the results of phase I human studies and animal experiments. Bleeding of preoperative anemia and/or surgical bleeding, Fluosol-DA 20% was administered to 7 of 10 Jehovah's Witness p...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.1984.tb04238.x
更新日期:1984-02-01 00:00:00
abstract::Only a small number of English hospitals provide postcardiotomy venoarterial extracorporeal membrane oxygenation (VA-ECMO) and there are doubts about its efficacy and safety. The aim of this service evaluation was to determine local survival rates and report on patient demographics. This was a retrospective service ev...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13647
更新日期:2020-07-01 00:00:00
abstract::We recently experienced a case of a female hemodialysis patient with multiple intestinal angiodysplasias. In 2001, she complained of melena, and angiographic embolization halted bleeding from ileum angiodysplasia. In 2002, she again complained of black stool. Abdominal angiography found jejunum angiodysplasia, and pha...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2004.29003.x
更新日期:2005-01-01 00:00:00
abstract::We aimed to determine the effect of elective left heart decompression at the time of initiation of central venoarterial extracorporeal membrane oxygenation (VA ECMO) on VA ECMO duration and clinical outcomes in children in a single tertiary ECMO referral center with a large pediatric population from a national referra...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12390
更新日期:2015-04-01 00:00:00
abstract::The in vitro development of a vascular stroma might be a solution for the engineering of vascularized tissues, however, in vitro stability of capillary-like structures is limited. In order to test the influence on maintenance of capillary-like structures, human growth hormone (hGH) was added in concentrations of 0.5, ...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/j.1525-1594.2005.29057.x
更新日期:2005-04-01 00:00:00
abstract::The accurate representation of rotary blood pumps in a numerical environment is important for meaningful investigation of pump-cardiovascular system interactions. Although numerous models for ventricular assist devices (VADs) have been developed, modeling methods for rotary total artificial hearts (rTAHs) are still re...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.12142
更新日期:2014-03-01 00:00:00
abstract::While pediatric HeartWare HVAD application has increased, determining candidacy and timing for initiation of pediatric VAD support has remained a challenge. We present our experience with a systematic approach to HVAD implantation as a bridge to pediatric heart transplantation. We performed a retrospective, single cen...
journal_title:Artificial organs
pub_type: 杂志文章
doi:10.1111/aor.13344
更新日期:2019-01-01 00:00:00