Abstract:
PURPOSE:To determine whether cigarette smoke condensate (CSC) without metabolic activation induces direct DNA double strand breaks (DSB) in the G1 phase of various radiosensitive mutants of CHO cells and whether these breaks display collateral hypersensitivity to CSC with respect to cell killing. MATERIALS & METHODS:We treated the G1-phase cultures of wild-type and DNA repair deficient mutants of CHO cells with various concentrations of CSC and examined the cell survival by colony formation assay and the induction of DNA double strand breaks by constant field gel electrophoresis as well as the phophorylated histone H2-A variant X (gamma-H2AX) assay. RESULTS:Gel analysis and gamma-H2AX focus assay showed significantly fewer, but still detectable levels of DSB per cell after CSC treatment compared to ionizing radiation (IR) exposures, even when equitoxic radiation exposures were delivered at a low dose rate over the same 8-hour exposure used for CSC treatments. None of the three non-homologous end joining (NHEJ) deficient mutants were remarkably hypersensitive to CSC compared to wild-type cells. In contrast, UV-1 cells that are hypersensitive to several base damage and cross-linking agents showed a higher sensitivity to CSC compared to the other CHO cell lines. CONCLUSIONS:DNA DSB produced directly by CSC are not principally responsible for its cytotoxicity. Further, the present study does not rule out the possibility that some of these lesions may secondarily result in DSB, such as may occur during impeded DNA replication and whose repair may require systems other than NHEJ.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Kato T,Nagasawa H,Warner C,Okayasu R,Bedford JSdoi
10.1080/09553000701481790subject
Has Abstractpub_date
2007-09-01 00:00:00pages
583-91issue
9eissn
0955-3002issn
1362-3095pii
780858278journal_volume
83pub_type
杂志文章abstract:PURPOSE:Radiation-induced bystander effects have important implications in radiotherapy. Their persistence in normal cells may contribute to risk of health hazards, including cancer. This study investigates the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of harmful e...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.950715
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206234
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To investigate alternative scenarios for the dose-dependent emission of bystander signals by irradiated cells in medium transfer experiments. METHODS:Energy deposition patterns to hypothetical intracellular targets whose hit by radiation initiates the emission of bystander signals have been simulated by Monte ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.595874
更新日期:2012-01-01 00:00:00
abstract:PURPOSE:To understand the mechanisms of life-shortening due to early neoplastic death caused by chronic low dose-rate (LDR; 20 mGy/22 h/day) radiation which accumulates to a high dose (HD; 8 Gy) (LDR/HD) as reported previously. MATERIALS AND METHODS:Female B6C3F(1) mice were continuously exposed to LDR/HD gamma-rays u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.545861
更新日期:2011-07-01 00:00:00
abstract::The relative contributions of the OH-mediated and direct radiation effect on the induction of DNA double-strand breaks (dsbs) were evaluated in two haploid yeast cell lines (GSH+ and gsh-) irradiated under oxic or hypoxic conditions in the absence or presence of 6 M glycerol as an OH radical scavenger. Gsh- cells are ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551721
更新日期:1993-11-01 00:00:00
abstract::Recently, it has been demonstrated that two different thermal resistant states found in Chinese hamster cells, one transient, associated with thermotolerance, and the other permanent, associated with the increased expression of the cognate member of the hsp 70 family, are characterized by faster recovery from heat-ind...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314450751
更新日期:1993-05-01 00:00:00
abstract:PURPOSE:To determine the increases in SSB and DSB yields after post gamma-irradiation incubation of plasmid DNA with the Escherichia coli base excision repair endonucleases formamidopyrimidine-DNA N-glycosylase (FPG) and endonuclease III (endo III). MATERIALS AND METHODS:Aqueous solutions of plasmid DNA were irradiate...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050176234
更新日期:2000-11-01 00:00:00
abstract:PURPOSE:The aim of this work is to study the relative damaging effects of DNA-incorporated radionuclides by analyzing the behavior of the liquid-scintillation counting efficiency in volumes of nanometer size. METHODS:A liquid scintillation counter can detect changes in the micelle size when different percentages of an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802460156
更新日期:2008-12-01 00:00:00
abstract:PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701570196
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:To investigate the ability of electromagnetic (EM) field pre-exposures to induce protection in chick embryos against subsequent ultraviolet (UV) light exposure. MATERIALS AND METHOD:Chick embryos in the 4th day of gestation were exposed for 20 minutes (short term) or 96 hours (long term) to 60 Hz, 8 microT mag...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139142
更新日期:1999-12-01 00:00:00
abstract:PURPOSE:To describe the design and dosimetric characterization of a simple and economical small animal irradiator. MATERIALS AND METHODS:A high dose rate (HDR) (192)Ir brachytherapy source from a commercially available afterloader was used with a 1.3 cm thick tungsten collimator to provide sharp beam penumbra suitable...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.922719
更新日期:2014-10-01 00:00:00
abstract:PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140474
更新日期:1999-03-01 00:00:00
abstract:PURPOSE:To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA. MATERIALS AND METHODS:Chromatin was isolated from calf thymus, freeze-dried and either used as such or ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050111541
更新日期:2000-08-01 00:00:00
abstract:PURPOSE:To investigate whether extremely low frequency electromagnetic field (ELF-EMF) exposure could induce oxidative stress in workers performing tour-inspection near transformers and distribution power lines. MATERIALS AND METHODS:Occupational short-term 'spot' measurements were performed. In total, 310 inspection ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1012304
更新日期:2015-05-01 00:00:00
abstract:PURPOSE:To determine the contribution of intra-alveolar cells as opposed to cells fixed in the interstitium in the development of radiation-induced lung injury. MATERIALS AND METHODS:C3H/HeN mice were irradiated to the thorax with various doses of radiation. The cellular composition and cytokine production were assess...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000076894
更新日期:2003-03-01 00:00:00
abstract::DNA double-strand break (dsb) induction in diploid yeast was measured by neutral sucrose sedimentation after exposure to very heavy ions with values of linear energy transfer (LET) ranging from about 300 to 11500 ke V/microns. Linear fluence dependencies were found in all cases from which dsb production cross-sections...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552121
更新日期:1992-09-01 00:00:00
abstract::Free radical formation in aqueous solutions of ubiquinone (coenzyme Qo) induced by ultrasound was studied by EPR and spin trapping with 3,5-dibromo-4-nitrosobenzene sulphonate. When aqueous solutions of ubiquinone were sonicated with 50-kHz ultrasound in the presence of argon or nitrogen, radicals formed by addition o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146246
更新日期:1996-01-01 00:00:00
abstract::Photoirradiation of aqueous solutions of DNA in the presence of Al- or Zn-tetrasulphonated phthalocyanines (AIPcS4 and ZnPcS4) causes formation of strand breaks and liberation of nucleobases. The effect of added D2O, which enhances singlet oxygen (1O2) lifetime, radical scavengers including alcohols and the spin-trap ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract:PURPOSE:To assess health, growth and reproductive success of mammals exposed for multiple generations to levels of radium-226 known to occur in environments surrounding uranium mines and mills in Canada. METHODS:The study consisted of a control group and four treatment groups each containing 40 mice (20 males and 20 f...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1028598
更新日期:2015-07-01 00:00:00
abstract::Purpose: Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1625462
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:To evaluate the radioprotective activity of C3, a regioisomer of water-soluble carboxyfullerene and a potent free radical scavenger, on both normal and tumour cells. MATERIALS AND METHODS:The murine committed bone-marrow stem cells for both granulocytes and monocytes (GM-CFC) were used to represent normal cell...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530001750068966
更新日期:2001-02-01 00:00:00
abstract:PURPOSE:To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data. MATERIALS AND METHODS:Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic exam...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141870
更新日期:1998-06-01 00:00:00
abstract::Radiation-induced myocardial degeneration in the rat is preceded by changes in capillary structure and function, which may be a major factor in the pathogenesis of radiation-induced heart disease. In order to investigate the mechanism of capillary damage we studied endothelial cell proliferation in untreated control r...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551131
更新日期:1990-05-01 00:00:00
abstract:PURPOSE:Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. MATERIALS AND METHODS:Paddy field microcosm samples were continuously exposed at a dose rate o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.549534
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:To investigate the hypothesis that the developmental phase at which an individual encounters radiation damage affects its long-term sensitivity to a subsequent tumourigenic insult. MATERIALS AND METHODS:Either the pregnant C57B16 mouse was exposed to 137Cs gamma-rays at 4 or 15 days post-conception (embryonic ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000139032
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of thi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1427903
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:The aim is to assess the change with age and disease of the oxygen concentration within the coronary artery walls. MATERIALS AND METHODS:In an accompanying paper, Part A, the age-dependent morphology and composition for the wall layers of normal and diseased coronary arteries were developed from published data...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802389645
更新日期:2008-10-01 00:00:00
abstract::Cell death induced by various radiation doses in Molt-4 and L5178Y cells was studied. Molt-4 cells have a low threshold for induction of interphase death while L5178Y cells have a high threshold. Interphase death in Molt-4 cells at doses > 100 Gy and in L5178Y cells at doses > 300 Gy showed typical necrosis. In contra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551101
更新日期:1993-07-01 00:00:00
abstract::The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145779
更新日期:1996-04-01 00:00:00
abstract:PURPOSE:The detection of long-term persistent chromosome aberrations in circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL AND METHODS:Peripheral blood samples from highly exposed persons were collected 7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and Chernobyl...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139395
更新日期:1999-10-01 00:00:00