Abstract：
:To assess the molecular basis of nalidixic acid and ciprofloxacin resistance in Helicobacter pullorum, the gyrA gene of H. pullorum CIP 104787T was sequenced. In addition, 9 isolates (2 susceptible to ciprofloxacin and resistant to nalidixic acid, 3 susceptible and 4 resistant to both antibiotics) were selected from 44 poultry isolates and the nucleotide sequences of their quinolone resistance-determining regions (QRDRs) were compared. The 2490 bp gyrA gene showed an open reading frame encoding a polypeptide of 829 amino acids. The deduced amino acid sequence of gyrA showed>or=72% identity to Helicobacter hepaticus, Helicobacter pylori and Wolinella succinogenes. Moreover, >or=98% amino acid sequence identity was found comparing the QRDR of the H. pullorum type strain with the QRDRs of the aforementioned bacterial species. All ciprofloxacin-resistant poultry isolates showed an ACA-->ATA (Thr-->Ile) substitution at codon 84 of gyrA, corresponding to codons 86, 87 and 83 of Campylobacter jejuni, H. pylori and Escherichia coli gyrA genes, respectively. This substitution was functionally confirmed to be associated with the ciprofloxacin-resistant phenotype of poultry isolates. This is the first report describing the complete 2490 bp nucleotide sequence of H. pullorum gyrA and confirming the involvement of the Thr84Ile substitution of GyrA in ciprofloxacin resistance of H. pullorum.
journal_name
Int J Antimicrob Agentsjournal_title
International journal of antimicrobial agentsauthors
Pasquali F,Rossi M,Manfreda G,Zanoni Rdoi
10.1016/j.ijantimicag.2007.04.017subject
Has Abstractpub_date
2007-09-01 00:00:00pages
222-8issue
3eissn
0924-8579issn
1872-7913pii
S0924-8579(07)00226-9journal_volume
30pub_type
杂志文章abstract：:The effect of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to uroepithelial cells of Escherichia coli strains was investigated. The hydrophobicity of the tested strains was evaluated by the bacterial adherence to hydrocarbon-xylene test and by the salt aggregation test...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2007.01.007
更新日期：2007-06-01 00:00:00
abstract：:The activity of telavancin was evaluated against Staphylococcus spp. collected from European hospitals as part of an international surveillance study (2007-2008). A total of 7534 staphylococcal clinical isolates [5726 Staphylococcus aureus and 1808 coagulase-negative staphylococci (CoNS)] were included. Isolates were ...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2010.05.016
更新日期：2010-10-01 00:00:00
abstract：:Acinetobacter baumannii has emerged as a major pathogen causing nosocomial infections, particularly in critical patients admitted to the Intensive Care Unit. Increasing resistance to carbapenems in A. baumannii has been observed worldwide. Here we report the clinical impact and mechanism of imipenem heteroresistance (...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2010.12.015
更新日期：2011-04-01 00:00:00
abstract：:The in vitro activity of moxifloxacin was compared with that of penicillin G, amoxycillin/clavulanate, cefoxitin, erythromycin, clindamycin and metronidazole against 158 isolates associated with periodontal infections. MIC(50)/MIC(90) values of moxifloxacin were respectively 0.06/0.5 mg/l for Porphyromonas gingivalis ...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/s0924-8579(02)00190-5
更新日期：2002-12-01 00:00:00
abstract：:Of the 16,024 patients hospitalized from January 1995 to October 1997, 397 (2.4%) acquired nosocomial pneumonia and the aetiological agent was defined in 101 (25%). About 82% developed late onset pneumonia (>7 days of hospitalization). The site of isolation of microorganisms was bronchoalveolar lavage (BAL) (49%), blo...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/s0924-8579(00)00316-2
更新日期：2001-02-01 00:00:00
abstract：:The spread of resistance to many antimicrobial agents in various microbial species has been highlighted by the World Health Organisation and many government agencies around the world. The reasons for this increase and spread are complex and are discussed. A number of surveillance studies has monitored the increase in ...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2003.08.005
更新日期：2003-10-01 00:00:00
abstract：:Synthesis and antibacterial screening of a homologous series of 3-dialkylaminophenothiazinium-7-norfloxacin conjugates was carried out alongside a corresponding series of symmetrical methylene blue derivatives. The norfloxacin conjugates maintained typical methylene blue derivative photoproperties, such as long wavele...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2009.11.022
更新日期：2010-04-01 00:00:00
abstract：:As treatment options for critically ill patients with multidrug-resistant bacteria diminish, older antibiotics such as fosfomycin are being investigated for use as last-resort drugs. Fosfomycin is a broad-spectrum antibiotic with activity both against Gram-positive and Gram-negative bacteria. The aim of this review wa...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2013.05.018
更新日期：2013-10-01 00:00:00
abstract：:The role of membrane permeabilisation and disruption in the mechanism of action of some polymyxin analogues against Gram-negative organisms is contentious. The effects of polymyxin B (PMB) and its analogue polymyxin B nonapeptide (PMBN) on Escherichia coli envelopes should correlate, but previous work by other workers...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2007.10.005
更新日期：2008-03-01 00:00:00
abstract：:Susceptibility testing was conducted on 1357 isolates of Neisseria gonorrhoeae isolated from 1993 through 2002 in Japan to assess the antimicrobial resistance. Selected isolates were characterised by auxotype and analysis was done for mutations within the quinolone resistance-determining region (QRDR) in the gyrA and ...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2004.02.005
更新日期：2004-09-01 00:00:00
abstract：:The aim of this study was to develop an in-vitro topical treatment for Acanthamoeba keratitis (AK) effective against cysts and trophozoites. Qualitative assays were performed with voriconazole, chlorhexidine, propamidine, cellulase, tobramycin, ciprofloxacin and paromomycin as monotherapy and various combinations. Rib...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2017.03.033
更新日期：2017-09-01 00:00:00
abstract：:Sepsis is a common and severe medical condition with substantial associated morbidity, mortality and cost. Furthermore, the incidence of sepsis has been rising annually over the past three decades, and morbidity and mortality remain high. The management of sepsis is further complicated by its very heterogeneous nature...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/S0924-8579(09)70560-6
更新日期：2009-01-01 00:00:00
abstract：:Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-KP) bloodstream infections (BSIs) are related to high mortality rates, and combination therapy has been associated with lower mortality in patients treated mostly with colistin. There is a paucity of studies addressing polymyxin B (PMB) treatment for KPC...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2018.10.010
更新日期：2019-02-01 00:00:00
abstract：:Carbapenem-resistant Enterobacteriaceae (CRE) are among the most difficult to treat emerging multidrug-resistant organisms. Major limitations exist in surveillance needed to address CRE, particularly in areas with inadequate resources. We utilised optimised strategies to search for data on carbapenem susceptibility of...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2018.05.019
更新日期：2018-09-01 00:00:00
abstract：:The pharmacist provides a vital role in the management of antibiotic policy. Consumers and payers demand quality care at a significantly reduced price. It is imperative that health care adapt to the times. Physicians must be involved in all phases of programmes that are viewed as cost saving. These programmes should i...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/s0924-8579(00)00250-8
更新日期：2000-11-01 00:00:00
abstract：:There is strong evidence that self-monitoring and feedback are effective behaviour change techniques (BCTs) across a range of healthcare interventions and that their effectiveness is enhanced by goal setting and action planning. Here we report a summary of the update of a systematic review assessing the application of...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2014.11.014
更新日期：2015-03-01 00:00:00
abstract：:Currently, only two drugs, i.e. ganciclovir, (DHPG, cytovene)_and foscarent (PFA, Foscavir), are licenced for the treatment of severe CMV infections. Both compounds may have serious side effects and can lead to the emergence of drug-resistant virus strains. Recent pertain to the efficacy of prophylactic or pre-emptive...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/0924-8579(93)90012-t
更新日期：1993-01-01 00:00:00
abstract：:Community-acquired pneumonia (CAP) is still one of the leading causes of mortality and morbidity. The most common bacterial cause of CAP is Streptococcus pneumoniae. The increase in antimicrobial resistance has raised concerns about the efficacy of available therapies, and a call for the reassessment of both existing ...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2004.08.006
更新日期：2004-11-01 00:00:00
abstract：:In the present study we tested the ability of beta-lactam antibiotics and other cell-wall antibiotics to inhibit the production of listeriolysin which is the main virulence factor of L. monocytogenes. The amount of listeriolysin produced was measured in the supernatants with a sensitive hemolysin assay and as beta-gal...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/0924-8579(96)00014-3
更新日期：1996-05-01 00:00:00
abstract：:The antimicrobial activity against Clostridium difficile of 109 lactic acid bacteria (LAB) isolated from 32 healthy Korean infants was measured. The ability to show similar activity against Escherichia coli O157:H7 and Staphylococcus aureus was also looked for. Twelve of the 109 LAB showed activity against C. difficil...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/s0924-8579(02)00389-8
更新日期：2003-04-01 00:00:00
abstract：:The aim of this study was to determine the clinical characteristics, molecular epidemiology and biofilm production of Acinetobacter baumannii clinical isolates obtained from a tertiary-care hospital in Mexico. Clinical isolates of A. baumannii (n=152) isolated from 2007 to 2012 were included. Clonal diversity was anal...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2014.10.022
更新日期：2015-03-01 00:00:00
abstract：:The epidemiology of invasive fungal infections is currently at a crucial stage. Potentially fatal fungal strains are emerging that are resistant to many commonly used antifungal agents. It is therefore important to recognize and minimize the major risk factors associated with infection. This paper summarizes the key e...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2006.03.006
更新日期：2006-06-01 00:00:00
abstract：:Antimicrobial agents (AMAs) have been used in agriculture since the 1950s as growth-promoting agents [antimicrobial growth promoters (AGPs)]. They have provided benefits to the agricultural industry by increasing production efficiencies and maximising livestock health, yet the potential risks surrounding resistance to...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2016.08.006
更新日期：2017-01-01 00:00:00
abstract：:In this study, the potency of DX-619, a novel des-fluoro(6)-quinolone agent, was compared with that of vancomycin (VCM) in a murine model of haematogenous bronchopneumonia infection caused by methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-intermediate S. aureus (VISA). The minimum inhibitory concent...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2006.03.026
更新日期：2006-09-01 00:00:00
abstract：:New initiatives have been introduced in Europe and the USA to encourage more rapid development of antibiotics. The need to ensure these new antibiotics can be safely used in children, and especially neonates, is important owing to high antimicrobial resistance in these patient groups. This review aims to determine wha...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
doi：10.1016/j.ijantimicag.2013.05.001
更新日期：2013-08-01 00:00:00
abstract：:Uptake of [14C]-azithromycin into THP-1 human monocytes was determined at pH 7.4, 6.8 or 5.5 over 4-log antibiotic concentrations for 24 h under a number of conditions. Stimulation of cells was with bacteria, latex beads, lipopolysaccharide (LPS), or zymogen A. Subcellular organelle disposition was determined after is...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/s0924-8579(02)00187-5
更新日期：2002-11-01 00:00:00
abstract：:Here we describe a patient undergoing extensive abdominal surgery and hyperthermic intraperitoneal chemotherapy due to primary adenocarcinoma in the sigmoid colon with peritoneal carcinomatosis. During hospitalisation the patient suffered from bacteraemia with a multidrug-resistant Bacteroides fragilis isolate. Whole-...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2015.02.024
更新日期：2015-07-01 00:00:00
abstract：:Using a murine model of disseminated infection by Fusarium verticillioides, the efficacy of liposomal amphotericin (L-AmB) B at 10mg/kg body weight once daily and terbinafine (TRB) at 150 mg/kg body weight twice daily, alone and in combination, was evaluated. The combination of L-AmB with TRB was the only treatment ab...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2010.08.008
更新日期：2011-01-01 00:00:00
abstract：:Modifications of the immune response have been reported for a number of antimicrobial agents following both in vivo and in vitro experiments. Present results were obtained from in vitro incubated human polymorphonuclear cells (PMN) and monocytes treated with meropenem, a novel carbapenem antibiotic. Only the highest c...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/0924-8579(94)00042-s
更新日期：1995-04-01 00:00:00
abstract：:The importance of the amino acid sequence in the C-terminal domain of penicillin-binding protein 5 (PBP5) and the levels of PBP5 expression to ampicillin resistance of Taiwan clinical isolates of Enterococcus faecium were studied. Sequence data revealed the existence of 12 amino acid sequence variants within the C-ter...
journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2006.07.027
更新日期：2006-12-01 00:00:00
