Abstract：
:Two forms of farnesyl diphosphate synthase (FPPS) from the spruce budworm, Choristoneura fumiferana, and one from the armyworm Pseudaletia unipuncta, have been cloned and their catalytic properties assessed. The type-2 FPPS of C. fumiferana (CfFPPS2) was efficient in the prenyl coupling of DMAPP or GPP with [(14)C]IPP, producing FPP as its final product; however, type-1 FPPSs (CfFPPS1, PuFPPS1, as well as Agrotis ipsilon FPPS1) were essentially inactive. A variety of purification methods was employed to purify the type-1 enzymes. Under mild chromatographic conditions, the isolated type-1 enzyme showed modest activity, but was apparently contaminated with endogenous prenyltransferase derived from the Escherichia coli host cells. Similarly, unpurified extracts of PuFPPS1 expressed in an E. coli FPPS-null mutant, had low FPPS activity. When equimolar amounts of homogenous CfFPPS1 and CfFPP2 were combined, a sharp synergistic enhancement of activity was observed, and the coupling of several homologous substrates, which are precursors to ethyl-branched JHs, was enhanced. Association between CfFPPS1 and CfFPPS2 was confirmed by both protein interaction chromatography and competitive ELISA. These data suggest that type-1 and type-2 FPPSs can form a heteromer, which may play a role in sesquiterpene biosynthesis, such as JH homologue formation, in moths.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Sen SE,Cusson M,Trobaugh C,Béliveau C,Richard T,Graham W,Mimms A,Roberts Gdoi
10.1016/j.ibmb.2007.05.012subject
Has Abstractpub_date
2007-08-01 00:00:00pages
819-28issue
8eissn
0965-1748issn
1879-0240pii
S0965-1748(07)00110-5journal_volume
37pub_type
杂志文章abstract：:Chagas disease or American trypanosomiasis, is a potentially life-threatening illness caused by the protozoan parasite, Trypanosoma cruzi. Once known as an endemic health problem of poor rural populations in Latin American countries, it has now spread worldwide. The parasite is transmitted by triatomine bugs, of which...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.06.005
更新日期：2016-02-01 00:00:00
abstract：:The midgut trehalase (THA) from fifth instar Lymantria dispar (gypsy moth) larvae was purified to homogeneity by two separate methods: gel filtration followed by Rotofor preparative IEF, and affinity chromatography on trehalose coupled to Sepharose 6B followed by preparative polyacrylamide gel electrophoresis. Midgut ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(93)90033-o
更新日期：1993-07-01 00:00:00
abstract：:A cholera toxin-sensitive, prostaglandin E2 (PGE2) specific receptor has been identified in the plasma membrane fraction of tick salivary glands. In the present study, we report that stimulation of dispersed salivary glands of the lone star tick Amblyomma americanum (L.) with 1 nM to 10 microM PGE2 increased the intra...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(98)00018-6
更新日期：1998-04-01 00:00:00
abstract：:A new member of the AKH/RPCH family was isolated and identified from the corpora cardiaca of the firebug Pyrrhocoris apterus. The peptide was isolated in a single step by reversed phase HPLC and the structure deduced from the multiple MS (MS(N)) electrospray mass spectra and amino acid analysis as that of an octapepti...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(00)00025-4
更新日期：2000-06-01 00:00:00
abstract：:Cyclorrhapha insect genomes contain a single acetylcholinesterase (AChE) gene while other insects contain at least two ace genes (ace1 and ace2). In this study we tested the hypothesis that the two ace paralogous from Blattella germanica have different contributions to AChE activity, using RNA interference (RNAi) to k...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2009.11.001
更新日期：2009-12-01 00:00:00
abstract：:Protein digestion in the lesser grain borer, Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), results from the action of a complex of serine proteinases present in the midgut. In this study we partially characterized trypsin-like enzyme activity against N-alpha-benzoyl-L-arginine p-nitroanilide (BApNA) in midgut ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00081-8
更新日期：1999-12-01 00:00:00
abstract：:Spodoptera littoralis is a phytophagous generalist. Its host range includes more than 40 plant species, some of which produce 3-nitropropanoic acid (3-NPA), an irreversible inhibitor of mitochondrial succinate dehydrogenase. Growth in larvae fed an artificial diet with a sublethal admixture of 3-NPA (4.2 μmol per g) w...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.05.013
更新日期：2015-08-01 00:00:00
abstract：:EFLamide (EFLa) is a neuropeptide known for a long time from crustaceans, chelicerates and myriapods. Recently, EFLa-encoding genes were identified in the genomes of apterygote hexapods including basal insect species. In pterygote insects, however, evidence of EFLa was limited to partial sequences in the bed bug (Cime...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2020.103376
更新日期：2020-07-01 00:00:00
abstract：:We cloned and characterized a novel Bombyx mori homologue (bm-dronc) of Drosophila melanogaster dronc (dm-dronc), which could encode a polypeptide of 438 amino acid residues. Bm-Dronc shares relatively low amino acid sequence identities of 25% and 26% with Dm-Dronc and Aedes aegypti Dronc (Aa-Dronc), respectively. Bm-...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2011.08.005
更新日期：2011-11-01 00:00:00
abstract：:Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in Manduca sexta. In this paper w...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(95)00077-1
更新日期：1996-01-01 00:00:00
abstract：:To understand the role that 20-hydroxy-ecdysone (20E) plays during ovarian development in Bombyx mori, we have undertaken the cloning of the silkworm ecdysone receptor (EcR) and a study of its expression during follicular cell differentiation. We have cloned a cDNA that contains a complete open reading frame for a 68....
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(95)00024-p
更新日期：1995-07-01 00:00:00
abstract：:Orcokinin (NFDEIDRSGFGFN) and orcokinin homologues are crustacean peptides eliciting potent myotropic effects in gut tissues. Through HPLC purification of brain extract of the cockroach Blattella germanica, we isolated the first insect orcokinin (NFDEIDRSGFNS). This insect orcokinin-like peptide do not show myotropic ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2004.07.005
更新日期：2004-11-01 00:00:00
abstract：:beta-Glucosidase activity [EC 3.2.1.21] was measured in the salivary glands and the gut of wood-eating termite, Neotermes koshunensis (Shiraki). 75% of the activity was detected in the salivary glands, whereas 15% of the activity was present in the hindgut, where numerous symbiotic flagellates reside. The salivary bet...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(02)00108-x
更新日期：2002-12-01 00:00:00
abstract：:Aminopeptidases N (APNs) are a class of ectoenzymes present in lepidopteran larvae midguts, involved in the Bacillus thuringiensis (Bt) toxins mode of action. In the present work, seven aminopeptidases have been cloned from the midgut of Ostrinia nubilalis, the major Lepidopteran corn pest in the temperate climates. S...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2010.04.010
更新日期：2010-07-01 00:00:00
abstract：:The brown planthopper, Nilaparvata lugens, is a model species for hemimetabolous development and the most important pest insect in rice, which is the major staple crop for about half of the world population. Despite its importance, little is known of the N-glycosylation process in this insect. Here we report on the N-...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.01.008
更新日期：2019-04-01 00:00:00
abstract：:Pyrethroids are an important class of insecticides for controlling insect pests, including the German cockroach. Unfortunately, many insects have developed resistance to pyrethroids. One of the most important mechanisms of resistance is kdr (knockdown resistance) which is characterized by neural insensitivity to pyret...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)90051-5
更新日期：1994-07-01 00:00:00
abstract：:As a component of the mediator complex, the intersex (ix) gene product is involved in the sex determination pathway of the Drosophila melanogaster. IX functions together with the female-specific product of doublesex (dsx) at the bottom of the hierarchy to implement female sexual differentiation. Here we analyzed the f...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.02.003
更新日期：2019-05-01 00:00:00
abstract：:Most moths utilize sex pheromones released by the female to attract a mate. Females produce the sex pheromone in the pheromone gland in a biosynthetic pathway which consists of several key enzymes. Fatty acyl-CoA reductase is one of the key enzymes, which catalyzes the conversion of fatty acyl-CoA to the corresponding...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2019.103260
更新日期：2020-01-01 00:00:00
abstract：:The male ejaculate contains a multitude of seminal fluid proteins (SFPs), many of which are key reproductive molecules, as well as sperm. However, the identification of SFPs is notoriously difficult and a detailed understanding of this complex phenotype has only been achieved in a few model species. We employed a rece...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.12.002
更新日期：2019-01-01 00:00:00
abstract：:The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also play...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2011.04.007
更新日期：2011-09-01 00:00:00
abstract：:The peach-potato aphid Myzus persicae (Sulzer) has developed resistance to pyrethroid insecticides as a result of a mechanism conferring reduced nervous system sensitivity, termed knockdown resistance (kdr). This reduced sensitivity is caused by two mutations, L1014F (kdr) and M918T (super-kdr), in the para-type volta...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2004.06.002
更新日期：2004-08-01 00:00:00
abstract：:The major beta-1,3-glucanase from Tenebrio molitor (TLam) was purified to homogeneity (yield, 6%; enrichment, 113 fold; specific activity, 4.4 U/mg). TLam has a molecular weight of 50 kDa and a pH optimum of 6. It is an endoglucanase that hydrolyzes beta-1,3-glucans as laminarin and yeast beta-1,3-1,6-glucan, but is i...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2009.10.003
更新日期：2009-12-01 00:00:00
abstract：:We report the insertion of the transposable element B104 in the Phenylalanine hydroxylase gene of the Drosophila mutant Henna-recessive 3. Its presence alters the Phenylalanine hydroxylase splicing pattern, producing at least two aberrant mRNAs which contain part of the B104 sequence interrupting the coding region. Th...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00002-8
更新日期：1999-04-01 00:00:00
abstract：:Sulfakinin (SK) is a sulfated insect neuropeptide that is best known for its function as a satiety factor. It displays structural and functional similarities with the vertebrate peptides gastrin and cholecystokinin. Peptidomic studies in multiple insects, crustaceans and arachnids have revealed the widespread occurren...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.03.008
更新日期：2015-06-01 00:00:00
abstract：:Two bisacylhydrazine insecticides with ecdysone-mimetic action, RH-5849 and RH-5992, have been subjected to several bioassay procedures that are prerequisites for ecdysone action in Drosophila larvae: (a) induction of early ecdysone-specific puffs on the polytene chromosomes of the larval salivary glands; (b) secretio...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/s0965-1748(99)00077-6
更新日期：1999-11-01 00:00:00
abstract：:A pheromonotropic peptide was isolated from brain-suboesophageal ganglion complexes of the adult female gypsy moth, Lymantria dispar, using a 5-step HPLC purification protocol and an in vivo bioassay in Helicoverpa zea. The intact peptide was sequenced by automated Edman degradation. The L. dispar pheromone biosynthes...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)90111-2
更新日期：1994-09-01 00:00:00
abstract：:It has long been suggested that phenoloxidases (POs) play key roles in various physiological functions in insects, e.g., cuticular sclerotization, wound healing, egg tanning and melanotic encapsulation of pathogens. Here we report that a mosquito PO, designated Armigeres subalbatus prophenoloxidase III (As-pro-PO III)...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2008.10.007
更新日期：2009-02-01 00:00:00
abstract：:The prothoracicotropic hormone (PTTH) stimulates ecdysteroidogenesis by prothoracic gland in larval insects. Previous studies showed that Ca(2+), cAMP, extracellular signal-regulated kinase (ERK), and tyrosine kinase are involved in PTTH-stimulated ecdysteroidogenesis by the prothoracic glands of both Bombyx mori and ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2010.12.004
更新日期：2011-03-01 00:00:00
abstract：:The multicopper oxidase (MCO) family of enzymes includes laccases, ascorbate oxidases, bilirubin oxidases and a subgroup of metal oxidases. On the basis of a bioinformatics investigation, we identified 7 genes encoding putative multicopper oxidase proteins in the genome of the brown planthopper (BPH), Nilaparvata luge...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2015.06.010
更新日期：2015-08-01 00:00:00
abstract：:The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a ...
journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/j.ibmb.2018.10.003
更新日期：2018-12-01 00:00:00
