Immobilization of mycobacterial cells onto silicone--assessing the feasibility of the immobilized biocatalyst in the production of androstenedione from sitosterol.

Abstract:

:Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Claudino MJ,Soares D,Van Keulen F,Marques MP,Cabral JM,Fernandes P

doi

10.1016/j.biortech.2007.05.007

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

2304-11

issue

7

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(07)00420-8

journal_volume

99

pub_type

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