Abstract:
:Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Claudino MJ,Soares D,Van Keulen F,Marques MP,Cabral JM,Fernandes Pdoi
10.1016/j.biortech.2007.05.007subject
Has Abstractpub_date
2008-05-01 00:00:00pages
2304-11issue
7eissn
0960-8524issn
1873-2976pii
S0960-8524(07)00420-8journal_volume
99pub_type
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