Abstract:
BACKGROUND:The process of tumour invasion and subsequent metastasis represents the most lethal aspect of cancer and is responsible for the majority of deaths among cancer patients. Our objective was to identify invasion-specific genes in a series of isogenic breast cancer cell lines of differing invasive potential using DNA microarray-based transcriptomic profiling. MATERIALS AND METHODS:A panel of increasingly invasive breast cancer subclones generated from the primary breast cancer cell line, Hs578T, were subjected to DNA microarray analysis using the Affymetrix HG-U133A array. RESULTS:Data analysis using GeneSpring 6.0 generated a list of 508 differentially expressed genes. A total of 71 genes were down-regulated and 437 genes were up-regulated. Array data for 17 genes was validated using quantitative real-time RT-PCR analysis. A correlation coefficient of 0.9249 was obtained when both technology platforms were compared. CONCLUSION:Potential new biomarkers for the diagnosis and treatment of invasive breast cancer have been identified.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Hughes L,O'Brien SL,Gallagher WM,Mcdonnell Ssubject
Has Abstractpub_date
2007-05-01 00:00:00pages
1353-9issue
3Aeissn
0250-7005issn
1791-7530journal_volume
27pub_type
杂志文章abstract::The specificities and surface markers of murine autocytotoxic cells induced by in vitro culture with interleukin 2 (IL2) were studied. Culturing murine spleen cells with recombinant human IL2 resulted in the generation of cytotoxic cells which killed syngeneic lymphoblasts and syngeneic activated macrophages (M phi). ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract:BACKGROUND:The aim of the study was to document the delay in diagnosis of Hodgkin's disease (HD) and clarify the impact on clinical stage and survival in our region. MATERIAL AND METHODS:A retrospective study of the records of 50 patients treated for primary HD in northern-Norway in the time period 1985 - 1993 was per...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract::Local control was compared between patients who had undergone breast-conserving therapy with and without nipple resection. We explored whether there was any difference in local control between the two treatment methods for patients with early breast cancer. A total of 333 women with breast cancer, who had undergone br...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::The in vitro antioxidative activity of benzylideneascorbate (SBA) and the in vivo effect on adriamycin (ADR)-induced cardiotoxicity in a mouse model were investigated. The radical-scavenging activity of SBA was assayed in terms of reduction of chemiluminescence induced by O2-, generated in xanthine/xanthine oxidase an...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract:AIM:To identify predictors of poor prognosis of patients with colon cancer (CC) who underwent surgery with curative intent, we investigated the association between the albumin to globulin ratio (AGR) with clinicopathological findings such as overall (OS) and disease-free (DFS) survival. PATIENTS AND METHODS:We conduct...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11452
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND/AIM:Hepatic venous isolation and extracorporeal charcoal hemoperfusion (HVI-CHP) can reduce systemic exposure to hepatic arterial infusion (HAI) chemotherapy. The pre-existing HVI-CHP system has limited effectiveness against high-dose cisplatin; therefore, we designed a new system and evaluated its efficacy ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13006
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND/AIM:To clarify whether renal dysfunction affects the incidence of adverse events associated with oxaliplatin, the present study was designed to investigate the relationship between creatinine clearance (Ccr) and the incidence of oxaliplatin-related adverse events. PATIENTS AND METHODS:A total of 287 CRC pat...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13953
更新日期:2020-01-01 00:00:00
abstract::Inositol hexakisphosphate (InsP6) inhibits serine/threonine protein phosphatases type-1 (PP1), type-2A (PP2A) and type-3 (PP3) in a concentration-dependent manner. Since the activity of voltage-gated L-type Ca(2+)-channels is increased by inhibition of serine/threonine protein phosphatases, this may explain trhe incre...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND/AIM:AZD8055 is an inhibitor of mammalian target of rapamycin (mTOR) that can suppress both mTOR complex 1 (mTORC1) and mTORC2. This study investigated the antitumor effects of AZD8055 on colon cancer. MATERIALS AND METHODS:The effects of AZD8055 on proliferation, apoptosis, and cell cycle of colon cancer ce...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12369
更新日期:2018-03-01 00:00:00
abstract:AIM:The present study investigated the clinical significance of tartrate-resistant acid phosphatase type-5 (ACP5) expression in gastric cancer. MATERIALS AND METHODS:In 150 specimens of gastric cancer and adjacent normal mucosa, expression of ACP5 protein and mRNA and was determined by immunohistochemical staining and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::Despite progress in chemotherapeutic agents, non-small cell lung cancers (NSCLC) still have a poor survival rate. Thus, development of new therapeutic strategies, specifically against cancer cells is still required. For this purpose, we treated the non-small cell lung cancer cell line NSCLC-N6 with the natural product...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-09-01 00:00:00
abstract::A universal intracellular factor, the "M phase-promoting factor" (MPF), triggers the G2/M transition of the cell cycle in all organisms. In late G2, it is present as an inactive complex of tyrosine-phosphorylated p34cdc2 and unphosphorylated cyclin Bcdc13. In M phase, its activation as an active MPF displaying histone...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-05-01 00:00:00
abstract::The electrophysiological effects of gallium chloride (Ga ) and its activity on arrhythmias induced by digitalis were investigated in guinea pig papillary muscle. KCl microelectrodes were used to record transmembrane electrical activity from Purkinje cells from the papillary muscle of guinea pig hearts during superfusi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::A total of 2090 patients with endometrial carcinoma were followed-up for at least five years. The treatment modalities, as well as the results of treatment, regarding 272 patients with disease relapse are presented. The results are not encouraging. We found no statistically significant difference regarding overall sur...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
abstract::A plethora of contradictory research surrounds vitamin D and its influence on health and disease. This may, in part, result from analytical difficulties with regard to measuring vitamin D metabolites in serum. Indeed, variation exists between analytical techniques and assays used for the determination of serum 25-hydr...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.12332
更新日期:2018-02-01 00:00:00
abstract::Many retrospective trials have demonstrated a survival benefit for elective lymph node dissection (ELND) in the treatment of clinical stage I malignant melanoma, but two randomized trials have found none. The relevant medical literature was reviewed with special attention devoted to the tumor burden of nodal disease. ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1995-03-01 00:00:00
abstract::Ten patients with advanced renal cell carcinoma were given combined chemoendocrine treatment with tegafur and tamoxifen. Patients were orally administered 800 mg/body tegafur and 20 mg/body tamoxifen daily. One patient was assessed as CR, three as PR, two as NC and four as PD. One out of two patients with estrogen rec...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND:Genetic alterations occurring in lung cancer are the basis for defining molecular subtypes and essential for targeted therapies. Exhaled breath condensate (EBC) is a form of non-invasive sample that, amongst components, contains DNA from pulmonary tissue. Next-generation sequencing (NGS) was herein used to a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12897
更新日期:2018-10-01 00:00:00
abstract::GAGE-1/-2 proteins are novel tumour markers, functionally related to tumour rejection. The objective of the present study was to identify the existence of a relationship between GAGE-1/-2 expression, Epstein Barr Virus (EBV) infection and viral infection-induced cytokine expression in cultivated tumour cells and archi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract:AIM:To determine depression in patients with choroidal melanoma (CM) treated with proton beam radiotherapy. PATIENTS AND METHODS:This was a cross-sectional study including 50 patients with CM (50% males, mean age=49.88±6.34 years) and 46 age- and sex-matched healthy controls (52% males, mean age=48.60±8.05 years). Par...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12562
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND:Preclinical studies suggest that 1,25-dihydroxyvitamin D [1,25(OH)2D] and celecoxib inhibit prostaglandins (PGs) associated with cancer through different mechanisms. We determined if there was synergy in their use. PATIENTS AND METHODS:A total of 36 healthy women received daily for one month/menstrual cycle...
journal_title:Anticancer research
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2013-09-01 00:00:00
abstract::Human primary breast cancers were analysed for somatic loss of heterozygosity (LOH) at chromosome 18 with 15 polymorphic microsatellite markers. LOH was observed in 148 of the 228 cases analyzed, (65%). Three smallest common deletion regions (SCDR) were detected on the long arm of chromosome 18. The marker D18S51 at t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::Seventy natural and synthetic compounds were tested for potential to inhibit mutation induced by 7,12-dimethylbenz(a)anthracene (DMBA) in Salmonella typhimurium strain TM677. Results were compared with their ability to inhibit DMBA-induced preneoplastic lesions in a mouse mammary gland organ culture system. The respon...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract:BACKGROUND:Neolymphangiogenesis, a process of lymphatic vessel development in neoplastic tissue, may be a key event in the transmission of cancer cells into lymph nodes. The current study examined the relationship between lymphatic vessel density (LVD) measured by podoplanin (D2-40) expression, clinicopathological para...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract:UNLABELLED:Distant metastases to the oral cavity, the face or facial skeleton are rare and/in are usually found in such locations at a late stage of the malignancy. In the majority of cases, the malignancy is already known at the time of admission. However, a distant metastasis as the first sign of a cancer developing ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-05-01 00:00:00
abstract::Our investigation comprises 778 cases of resection for early gastric cancer (27.4% of all stomach resections). The rate of lymph node metastasis was 1.2% in m (mucosal cancer) group, 3.3% in sm (submucosal cancer) 1 group and 22.6% in sm 2 group. In sm 2 group, lymph node extirpation of R2 is essential. The five year ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::Mutations in the p53 gene stimulate cell division. In our study we assessed the prognostic value of mutant p53 overexpression in cervical cancer stage FIGO III detected by immunohistochemistry. In 43 tissue specimens were detected p53 overexpression in 20 cases. Mean survival time was 36.4 (SE +/- 7.66) months in the ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::The effect on liver tissue of glutathione administration to rats treated for 7-14 days with 2-acetylaminofluorene was investigated. The DNA damage induced by the hepatotoxic agent and evaluated by the alkaline elution technique was significantly reduced by glutathione. Furthermore, GSH administration maintained liver ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
abstract::Non-small-cell lung cancer (NSCLC) ranks as a leading cause of cancer-related death globally. Brain metastases are a frequent complication of NSCLC, with 25-40% of patients developing brain metastases during the course of the disease, often within the first 2 years after diagnosis of the primary tumor. Improvements in...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2015-11-01 00:00:00
abstract::In the present work, we studied the expression of the c-myc oncoprotein p-62 and the ras oncoprotein p-21 in the dermal cellular infiltrate of paraffin embedded skin specimens, obtained from patients suffering from Mycosis Fungoides and Sezary syndrome. Nineteen specimens from early stage Mycosis Fungoides, nineteen f...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00