Abstract:
:We propose a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) via coculture with murine fetal liver-derived stromal cells, in which embryonic hematopoiesis dramatically expands at midgestation. We generated various hematopoietic progenitors in coculture, and this hematopoietic activity was concentrated in cobblestone-like cells derived from differentiated hESC. The cobblestone-like cells mostly expressed CD34 and retained an endothelial cell potential. They also contained hematopoietic colony-forming cells, especially erythroid and multilineage colony-forming cells at high frequency. The multipotential hematopoietic progenitors abundant among the cobblestone-like cells produced almost all types of mature blood cells, including adult-type alpha-globin-expressing erythrocytes and tryptase/chymase double-positive mast cells. These progenitors showed neither the immature properties of ESC nor the potential to differentiate into endoderm and ectoderm at a clonal level. The coculture system developed for hESC can provide a novel source of hematopoietic and blood cells for applications in cellular therapy and drug screening.
journal_name
Int J Hematoljournal_title
International journal of hematologyauthors
Ma F,Wang D,Hanada S,Ebihara Y,Kawasaki H,Zaike Y,Heike T,Nakahata T,Tsuji Kdoi
10.1532/IJH97.06203subject
Has Abstractpub_date
2007-06-01 00:00:00pages
371-9issue
5eissn
0925-5710issn
1865-3774pii
P068366614P73432journal_volume
85pub_type
杂志文章abstract::We report a case of primary extramedullary plasmacytoma (PEMP), IgA kappa type, with a long clinical history, in a 64-year-old Japanese woman. In this case, PEMP occurred primarily in the jejunum and then recurred in several organs, specifically the spleen and stomach, and in the subcutaneous tissue, over a period of ...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Severe combined immunodeficiency (SCID) conditions appear to be the best possible candidates for a gene therapy approach. Transgene expression by lymphocyte precursors should confer to these cells a selective growth advantage that gives rise to long-lived T-lymphocytes. This rationale was used as a basis for a clinica...
journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
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journal_title:International journal of hematology
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journal_title:International journal of hematology
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abstract::Recent studies have shown that tumors of relapsed acute myeloid leukemia (AML) present additional genetic mutations compared to the primary tumors. The base excision repair (BER) pathway corrects oxidatively damaged mutagenic bases and plays an important role in maintaining genetic stability. The purpose of the presen...
journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
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更新日期:2006-04-01 00:00:00
abstract::Nuclear factor-kappa B (NF-κB) is a multipotent transcription factor that plays a pivotal role in immune reactions, inflammation, and possibly hematopoiesis as well. Mobilization of neutrophilic granulocytes during inflammation is a highly regulated process, but one that is incompletely understood. We studied the in v...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-014-1598-7
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journal_title:International journal of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s12185-017-2311-4
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
doi:10.1007/s12185-018-2403-9
更新日期:2018-04-01 00:00:00
abstract::Osteonecrosis (ON) is a potentially disabling complication encountered in children who receive chemotherapy for acute lymphoblastic leukemia (ALL). Considering the possible effect of ethnic difference on the clinical features of symptomatic ON in pediatric ALL, we retrospectively evaluated 245 children with ALL who we...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-015-1790-4
更新日期:2015-07-01 00:00:00
abstract::Cytomegalovirus (CMV) infection is latent in the majority of adult humans. The reactivation of CMV causes pneumonia and gastrointestinal disease in severely immunosuppressed patients, who consequently suffer very high mortality due to CMV central nervous system disease. We report here a case involving a 28-year-old fe...
journal_title:International journal of hematology
pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
abstract::Chronic lymphocytic leukemia is one of the most common leukemias in the western world and consists of many chromosome aberrations. We report the case of a 74-year-old male patient with chronic lymphocytic leukemia with complex variant translocations t(8;22)(q24;q11) and der(8)t(6;8)(p21;p21) identified by chromosome b...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1532/ijh97.a10323
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abstract::To determine the pathogenesis of hemophagocytic lymphohistiocytosis (HLH), serum levels of neuron-specific enolase (NSE) and cytokine profiles were investigated. Serum concentrations of NSE and several cytokines were measured by immunoassays, and the association was evaluated in 18 HLH patients. Serum NSE levels incre...
journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1532/IJH97.A30504
更新日期:2005-10-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
doi:10.1007/s12185-018-2507-2
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journal_title:International journal of hematology
pub_type: 杂志文章
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
doi:
更新日期:1992-06-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章,多中心研究
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abstract::The NOTCH and nuclear factor kappa B (NF-κB) pathways are both constitutively activated in Chronic Lymphocytic Leukemia (CLL). We first described the NOTCH1 PEST domain mutation in a CLL subgroup, but the activation of the NOTCH pathway in NOTCH1-unmutated cases remains unexplained. Here, we investigated whether genet...
journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:
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journal_title:International journal of hematology
pub_type: 杂志文章
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journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
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abstract::Multiple myeloma is a disease involving the clonal evolution of plasma cells that produce monoclonal immunoglobulin; however, other products, such as ammonia and amylase, reportedly are secreted by neoplastic plasma cells. We describe a patient with immunoglobulin A (IgA) myeloma who showed a high serum level of carci...
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pub_type: 杂志文章
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