Abstract:
BACKGROUND:The objective of this study was to assess the rate and possible reasons for false-negative (FN) reflex human papillomavirus (HPV)-DNA tests. METHODS:The authors reviewed all ThinPrep cervical specimens that were submitted for reflex HPV-DNA testing using the Digene Hybrid Capture II (HC2) method from January 2002 to January 2004. Follow-up biopsies were reviewed. The results were considered HPV-FN if the HPV-DNA test was negative and the biopsy was positive for grade > or =2 cervical intraepithelial neoplasia (CIN2+), and the results were considered true positive (HPV-TP) if the HPV-DNA test was positive and the biopsy showed CIN2+. HPV-FN cases were compared with HPV-TP cases regarding the grade and extent of CIN, the number of abnormal cells on the original ThinPrep slide, and the presence of amplifiable, viral DNA on biopsy. RESULTS:In total, 1520 (66%) of 2309 patients who had diagnoses of atypical squamous cells of undetermined significance (ASCUS) were negative for HPV DNA and 789 patients of 2309 patients (34%) were positive for HPV DNA. Three hundred sixteen women (40%) who had a positive HPV-DNA test underwent a biopsy. Of those, 36 biopsies (11%) showed CIN2+ (HPV-TP), and 154 biopsies (66%) showed CIN1. Cervical tissue was available for review from 82 women who had negative HPV-DNA tests; of these, 6 tissue samples (7%) showed CIN2+ (HPV-FN), and 13 tissue samples (16%) showed CIN1. Therefore, in the total ASCUS population that was triaged with reflex HPV testing, there were at least 42 women who were diagnosed with CIN2+, for an estimated CIN2+ FN fraction of 14% (6 of 42 women). HPV-FN lesions were smaller (but the difference was not statistically significant) and shed significantly fewer abnormal cells than HPV-TP cases. Polymerase chain reaction testing for viral DNA in the biopsy was detected in 3 of 6 women who had HPV-FN results; none of those positive results demonstrated a viral type that was not included in the Digene probes. CONCLUSIONS:Although the rate of FN high-grade lesions was significantly higher than that reported in the ASCUS/Low-grade Squamous Intraepithelial Lesion Triage trial, most missed lesions were small and shed few abnormal cells. It was assumed that those lesions were either in early stages or in regressing stages, which made their clinical significance uncertain.
journal_name
Cancerjournal_title
Cancerauthors
Eltoum IA,Chhieng DC,Crowe DR,Roberson J,Jin G,Broker TRdoi
10.1002/cncr.22688subject
Has Abstractpub_date
2007-06-25 00:00:00pages
154-9issue
3eissn
0008-543Xissn
1097-0142journal_volume
111pub_type
杂志文章相关文献
CANCER文献大全abstract:BACKGROUND:Multimodality treatment has dramatically improved the outcome of patients with Ewing sarcoma. However, there appears to be little information concerning treatment-related complications in patients who are long-term survivors. METHODS:Forty-one patients with Ewing sarcoma who were treated between 1960-1980 a...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.11891
更新日期:2003-12-15 00:00:00
abstract::To examine the relationship between smoking and stage of colorectal cancer at diagnosis, data on 2788 current smokers and 6313 nonsmokers with known stage at diagnosis were obtained from the Massachusetts Cancer Registry. The stage at diagnosis was treated as a polytomous response variable in a logit-type, log-linear ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19890915)64:6<1372::aid-cncr2820
更新日期:1989-09-15 00:00:00
abstract:BACKGROUND:Previous studies have suggested that the prognosis in patients with extremity soft-tissue sarcomas (ESTS) with isolated lymph node (LN) metastases (stage IV) is comparable to that of patients with high-risk ESTS without metastases (stage III). This study was performed to determine the outcomes of patients wh...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.23363
更新日期:2008-04-15 00:00:00
abstract:BACKGROUND:This study explores whether externalizing religious and spiritual beliefs is associated with advanced-stage colon cancer at initial oncology presentation and whether this association is stronger for blacks than for whites. METHODS:Patients who had newly diagnosed, invasive colon cancer were recruited at 9 s...
journal_title:Cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/cncr.31351
更新日期:2018-06-15 00:00:00
abstract::The authors report the case of a child with retroperitoneal ganglioneuroma and cytodifferentiated skeletal metastases. The primary tumor was surgically resected, and the child is alive and well 2 years later without additional therapy. This rarely documented phenomenon can be explained by spontaneous cytomaturation wi...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19840715)54:2<357::aid-cncr28205
更新日期:1984-07-15 00:00:00
abstract:BACKGROUND:The reliability of fine-needle aspiration cytology (FNAC) of breast masses in males could be compromised by lack of experience because breast carcinoma is rare in males and FNAC is not often used. In addition, FNAC of the more often encountered gynecomastia may lead to overdiagnosis. Therefore, in the curren...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.10483
更新日期:2002-04-25 00:00:00
abstract:BACKGROUND:The axon guidance cue molecule Slit2 has been shown to suppress cancer cell invasion. However, the role of Slit2 in growth inhibition is still controversial. The authors identified a novel exon 15 (AKEQYFIP)-deleted slit2, located at the end of the second leucine-rich repeat (LRR2). Because LRR2 interacts wi...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.25890
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:During a population-based local cancer registry, a peculiar type of T-cell lymphoma restricted to the gastrointestinal tract was found in patients living in southwestern Japan. METHODS:Five cases of gastrointestinal (GI) tract T-cell lymphoma were analyzed with immunohistologic examination, ultrastructural ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19930201)71:3<708::aid-cncr28207
更新日期:1993-02-01 00:00:00
abstract::Proviral DNA of adult T-cell leukemia virus (HTLV-I) was examined by the standard Southern blotting method in lymph nodes of 45 patients with anti-HTLV-I antibody (ATLA)-positive adult T-cell leukemia/lymphoma (ATLL). Six of these patients revealed no monoclonal proviral HTLV-I DNA in tumor cells. These six patients s...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19891215)64:12<2515::aid-cncr282
更新日期:1989-12-15 00:00:00
abstract:BACKGROUND:The prognostic significance of molecular phenotype in breast cancer is well established in the literature. Recent studies have demonstrated that subgross lesion distribution (unifocal, multifocal, and diffuse) and disease extent also carry prognostic significance in this disease. However, the correlation of ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.27877
更新日期:2013-03-15 00:00:00
abstract::Between 1970 and 1989, 17 children with histologically malignant intracranial ependymomas received treatment at the University of Pennsylvania (Philadelphia, PA). Eleven were treated with prophylactic cranial or craniospinal irradiation plus a local boost (CS-XRT), five with local (L-XRT) irradiation only, and one was...
journal_title:Cancer
pub_type: 临床试验,杂志文章
doi:10.1002/1097-0142(19910601)67:11<2766::aid-cncr282
更新日期:1991-06-01 00:00:00
abstract::Interleukin (IL)-2 therapy leads to respiratory dysfunction due to increased vascular permeability. This study examines the role of the chemoattractant, immunomodulator, and permeability-promoting agent leukotriene (LT) B4 in this setting. Sheep with chronic lung lymph fistulae were given IL-2, 10(5) U/kg as an IV bol...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/1097-0142(19901201)66:11<2357::aid-cncr282
更新日期:1990-12-01 00:00:00
abstract::The results of surgical treatment alone for 142 cases of carcinoma of the rectum and rectosigmoid from the Massachusetts General Hospital were reviewed. The incidence of local failure as any component of failure was found to be strongly dependent on the pathologic stage, and for Dukes' A was 8.0% (3/39); Dukes' B, 31%...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19831001)52:7<1317::aid-cncr2820
更新日期:1983-10-01 00:00:00
abstract::Eight cases of primary non-Hodgkin's lymphoma of the breast found in the pathology files of the Institute of Oncology, Ljubljana, Yugoslavia, for a period of 25 years (from 1961 to 1985) were analyzed. During the same period 5711 cases of breast carcinoma were seen. Seven cases were diffuse lymphomas, and one case was...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/1097-0142(19871215)60:12<3033::aid-cncr282
更新日期:1987-12-15 00:00:00
abstract::A 21-year-old patient, after radiation therapy to the mediastinum for Hodgkin's disease, died six years later of a spinal cord glioma, believed to be caused by irradiation therapy. That the x-ray therapy provoked neoplastic changes seems likely, although it could be coincidental. ...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19800415)45:8<2051::aid-cncr2820
更新日期:1980-04-15 00:00:00
abstract:BACKGROUND:Helicobacter pylori infection is a risk factor for gastric carcinogenesis. High dietary vitamin C intake appears to protect against gastric carcinoma. It has been suggested that vitamin C exerts the protective effect by scavenging free radicals that may be enhanced by H. pylori. However, vitamin C has not be...
journal_title:Cancer
pub_type: 杂志文章
doi:
更新日期:1997-11-15 00:00:00
abstract::Thrombocytosis above 40.0 X 10(4)/mm3 occurred in five of six (83%) patients with malignant mesothelioma. In contrast, the incidence of thrombocytosis was 7.5% in patients with lung adenocarcinoma, 12.5% in squamous cell carcinoma, 5.1% in small cell carcinoma, and 41.7% in large cell carcinoma, respectively. The plat...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19861015)58:8<1699::aid-cncr2820
更新日期:1986-10-15 00:00:00
abstract::Jaundice and hepatomegaly developed in a boy with Fanconi anemia after he had undergone treatment with oxymetholone for nine years. A liver scan showed patchy uptake consistent with the presence of space-occupying lesions. After oxymetholone treatment was stopped, the jaundice resolved, the liver size decreased, and t...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19800915)46:6<1401::aid-cncr2820
更新日期:1980-09-15 00:00:00
abstract:BACKGROUND:Because studies have suggested that anemia has an adverse effect on outcome for patients with cervical carcinoma who are treated with radiation, recombinant human erythropoietin (rHuEpo) has been used increasingly to maintain hemoglobin levels in these patients. Erythropoietin may increase the risk of thromb...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.11700
更新日期:2003-10-01 00:00:00
abstract:BACKGROUND:In this report, the authors present the results from a study of patients with unresectable oropharyngeal squamous cell carcinomas who were treated on a protocol of hyperfractionated radiation and high-dose intraarterial cisplatin (HYPERRADPLAT) at the University of Kentucky. METHODS:The study was designed a...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.21368
更新日期:2005-10-15 00:00:00
abstract:BACKGROUND:Factors contributing to the lower likelihood of urologist follow-up among African American (AA) men diagnosed with prostate cancer may not be strictly related to patient factors. The authors investigated the relationship between crime, poverty, and poor housing, among others, and postdiagnosis urologist visi...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.28894
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:Tyrosine kinase inhibitors (TKIs) have improved the outcome of patients with gastrointestinal stromal tumors (GISTs), but most patients eventually develop resistance and progress. Dasatinib is a potent inhibitor of BCR-ABL, KIT, and SRC family kinases as well as imatinib-resistant cells. In GISTs, response e...
journal_title:Cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/cncr.31234
更新日期:2018-04-01 00:00:00
abstract::Extensive bone marrow necrosis is an unusual histologic finding most commonly identified after autopsy. We describe a patient with poorly differentiated lymphocytic lymphoma who manifested pancytopenia and marrow necrosis. Bone marrow scanning with 99mTc-sulfur-minicolloid provided a noninvasive means to assess the ex...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19801115)46:10<2168::aid-cncr282
更新日期:1980-11-15 00:00:00
abstract:BACKGROUND:A hypothesis generated a retrospective analysis of the improvement of survival over time in patients with cutaneous melanoma (CM) in order to identify factors contributing to this progress. METHODS:A cohort of 4791 patients diagnosed with primary CM in southern Germany between 1976 and 2001 was analyzed. Ka...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/cncr.22511
更新日期:2007-03-15 00:00:00
abstract:BACKGROUND:The pretherapy prediction of occult lymph node involvement and the avoidance of otherwise futile and potentially morbid definitive local therapy is paramount in men with newly diagnosed prostate carcinoma. To identify patients with prostate carcinoma who likely have lymph node involvement and would benefit f...
journal_title:Cancer
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Eleven patients with advanced soft tissue sarcoma were treated with whole body hyperthermia (41.8 degrees C-43.0 degrees C) for 2 hours, doxorubicin (45 mg/m2) at the beginning of peak temperature and cyclophosphamide (1000 mg/m2) 6 hours after doxorubicin. Warming was accomplished with a nylon and vinyl mesh water pe...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19840615)53:12<2585::aid-cncr282
更新日期:1984-06-15 00:00:00
abstract:BACKGROUND:Laryngeal cancer is the primary cause of death among patients with head and neck cancer in Argentina. Different prognostic factors have been incidentally dealt with in a number of articles, but there are few studies of prognostic variables, such as age, tobacco, and alcohol, using multifactorial analysis. M...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19930415)71:8<2472::aid-cncr2820
更新日期:1993-04-15 00:00:00
abstract::Sixty patients with advanced prostatic carcinoma were treated with monthly subcutaneous injections of a depot formulation of goserelin, a luteinizing hormone-releasing hormone (LHRH) analogue (Zoladex, ICI Pharma, Destelbergen, Belgium). All patients were regularly evaluated with measurements of luteinizing hormone (L...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19910201)67:3<557::aid-cncr28206
更新日期:1991-02-01 00:00:00
abstract::A case of malignant eccrine poroma with lymph node metastases is described. The patient had a nodular growth of the right thigh 2 years before its first excision. Local recurrence in the scar occurred in 6 months, and by 8 months a right groin lymph node was involved. Subsequent skin nodules of the right thigh occurre...
journal_title:Cancer
pub_type: 杂志文章,评审
doi:10.1002/1097-0142(19840401)53:7<1579::aid-cncr2820
更新日期:1984-04-01 00:00:00
abstract::Interest in the elderly population, persons 65 years of age or older, as a target group for cancer control has not been great. Age recommendations rarely are made for prevention and early detection of cancer for elderly persons. However, cancer incidence and mortality rates are known to rise rapidly with increasing ag...
journal_title:Cancer
pub_type: 杂志文章
doi:10.1002/1097-0142(19881015)62:1+<1823::aid-cncr282
更新日期:1988-10-15 00:00:00