Detection of cathepsin S cysteine protease in human brain tumour microdialysates in vivo.

Abstract:

:Microdialysis enables the chemistry of extracellular fluid in body tissues to be measured. Extracellular proteases such as the cysteine protease, cathepsin S (CatS), are thought to facilitate astrocytoma invasion. Microdialysates obtained from human brain tumours in vivo were subjected to cathepsin S activity and ELISA assays. Cathepsin S ELISA expression was detected in five out of 10 tumour microdialysates, while activity was detected in five out of 11 tumour microdialysates. Cathepsin S expression was also detected in microdialysate from the normal brain control although no activity was found in the same sample. While some refinements to the technique are necessary, the authors demonstrate the feasibility and safety of microdialysis in human astrocytomas in vivo. Characterisation of the extracellular environment of brain tumours in vivo using microdialysis may be a useful tool to identify the protease profile of brain tumours.

journal_name

Br J Neurosurg

authors

Flannery T,McConnell RS,McQuaid S,McGregor G,Mirakhur M,Martin L,Scott C,Burden R,Walker B,McGoohan C,Johnston PG

doi

10.1080/02688690701248190

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

204-9

issue

2

eissn

0268-8697

issn

1360-046X

pii

777021244

journal_volume

21

pub_type

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