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Standardised assessment of membrane proteinase 3 expression. Analysis in ANCA-associated vasculitis and controls.

Abstract：

OBJECTIVES:Increased numbers of neutrophils expressing proteinase 3 on their membrane (mPR3) have been reported in anti-neutrophil cytoplasm antibody (ANCA)-associated vasculitis (AAV) and are suggested to be involved in AAV immunopathogenesis. In most studies, neutrophils were analysed for mPR3 expression without priming with TNFalpha, suggesting that mPR3 expression on neutrophils is dependent on other priming events, such as isolation procedures . These priming events can be variable. Therefore, we analysed mPR3 expression on neutrophils before and after priming with TNFalpha to assess whether standardised assessment of mPR3 expression requires priming. Using neutrophils before and after priming with TNFalpha, we assessed percentages of mPR3(+) neutrophils in patients with AAV and in disease and healthy controls. METHODS:Neutrophils from patients with PR3-AAV and MPO-AAV, systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), and from healthy controls were analysed before and after priming with TNFalpha for mPR3 expression. RESULTS:42% of all individuals analysed showed minimal expression for mPR3 on all neutrophils before priming with TNFalpha, whereas after priming a clear mPR3(+) subset was observed next to mPR3(-) neutrophils, corresponding to bimodal mPR3 expression. In patients with PR3-AAV or MPO-AAV, the percentage of mPR3(+) neutrophils after priming with TNFalpha was significantly increased (p<0.01 and p<0.05, respectively) compared with healthy controls. Percentages of mPR3(+) PMN were also increased in patients with SLE (p<0.01) but not in RA. CONCLUSION:Standardised assessment of proteinase 3 on the membrane of neutrophils requires priming with TNFalpha. Percentages of mPR3(+) PMN are increased in AAV and SLE, but not in RA.

journal_name

Ann Rheum Dis

journal_title

Annals of the rheumatic diseases

authors

van Rossum AP,Huitema MG,Stegeman CA,Bijl M,de Leeuw K,Van Leeuwen MA,Limburg PC,Kallenberg CG

doi

10.1136/ard.2006.063230

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

1350-5

issue

eissn

0003-4967

issn

1468-2060

pii

ard.2006.063230

journal_volume

pub_type

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Standardised assessment of membrane proteinase 3 expression. Analysis in ANCA-associated vasculitis and controls.