Abstract：
:Terminal restriction fragment length polymorphism (T-RFLP) is an increasingly widely used technique in mycorrhizal ecology. In this paper, we review the technique as it is used to identify species of mycorrhizal fungi and distinguish two different versions of the technique: peak-profile T-RFLP (the original version) and database T-RFLP. We define database T-RFLP as the use of T-RFLP to identify individual species within samples by comparison of unknown data with a database of known T-RFLP patterns. This application of T-RFLP avoids some of the pitfalls of peak-profile T-RFLP and allows T-RFLP to be applied to polyphyletic functional groups such as ectomycorrhizal fungi. The identification of species using database T-RFLP is subject to several sources of potential error, including (1) random erroneous matches of peaks to species, (2) shared T-RFLP profiles across species, and (3) multiple T-RFLP profiles within a species. A mathematical approximation of the risk of the first type of error as a function of experimental parameters is discussed. Although potentially less accurate than some other methods such as clone libraries, the high throughput of database T-RFLP permits much greater replication and may, therefore, be preferable for many ecological questions, particularly when combined with other techniques such as cloning.
journal_name
Mycorrhizajournal_title
Mycorrhizaauthors
Dickie IA,FitzJohn RGdoi
10.1007/s00572-007-0129-2subject
Has Abstractpub_date
2007-06-01 00:00:00pages
259-270issue
4eissn
0940-6360issn
1432-1890pii
10.1007/s00572-007-0129-2journal_volume
17pub_type
杂志文章,评审相关文献
MYCORRHIZA文献大全abstract：:The development of arbuscular mycorrhiza (AM) is strongly suppressed under high-phosphate (Pi) conditions. To investigate AM fungal responses during the suppression of AM by high Pi, we performed an RNA-seq analysis of Rhizophagus irregularis colonizing Lotus japonicus roots at different levels of Pi (20, 100, 300, an...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-016-0735-y
更新日期：2017-02-01 00:00:00
abstract：:Ink disease caused by Phytophthora cambivora is a major disease of sweet chestnut (Castanea sativa). In two C. sativa stands in central Italy, one (Montesanti) that is infected with P. cambivora and the trees showing symptoms of ink disease and another healthy stand (Puzzella), the ectomycorrhizal (ECM) community stru...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-009-0256-z
更新日期：2009-11-01 00:00:00
abstract：:Forest ecosystems of the Pacific Northwest of the USA are changing as a result of climate change. Specifically, rise of global temperatures, decline of winter precipitation, earlier loss of snowpack, and increased summer drought are altering the range of Pinus contorta. Simultaneously, flux in environmental conditions...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-015-0668-x
更新日期：2016-05-01 00:00:00
abstract：:The Périgord black truffle (Tuber melanosporum Vittad.) is an ectomycorrhizal fungus forming edible fructifications. The production of T. melanosporum relies mainly on man-made plantations. T. melanosporum is a heterothallic species requiring the meeting of two partners of opposite mating types to fruit. It is common ...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-020-01011-4
更新日期：2021-01-09 00:00:00
abstract：:Arbuscular mycorrhiza fungi (AMF) are beneficial soil fungi that can promote the growth of their host plants. Accurate quantification of AMF in plant roots is important because the level of colonization is often indicative of the activity of these fungi. Root colonization is traditionally measured with microscopy meth...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-020-01014-1
更新日期：2021-01-21 00:00:00
abstract：:Long-lived radionuclides such as (90)Sr and (137)Cs can be naturally or accidentally deposited in the upper soil layers where they emit β/γ radiation. Previous studies have shown that arbuscular mycorrhizal fungi (AMF) can accumulate and transfer radionuclides from soil to plant, but there have been no studies on the ...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-015-0664-1
更新日期：2016-04-01 00:00:00
abstract：:Plantation forestry with exotic trees in south China needs compatible symbionts to improve the growth of seedlings in nurseries and to enhance establishment and growth in the field. Scleroderma, a potentially suitable symbiont for inoculation, is not being used in containerized nurseries in the region due to poor know...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-006-0039-8
更新日期：2006-06-01 00:00:00
abstract：:Nontimber forest products (NTFPs) represent an important source of income to millions of people in tropical forest regions, but some NTFP species have decreased in number and become endangered due to overexploitation. There is increasing concern that the planting stocks of Dyera polyphylla and Aquilaria filaria are no...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-006-0059-4
更新日期：2006-10-01 00:00:00
abstract：:There is increasing interest in the quality of crops because of the implications concerning health, economic revenue, and food quality. Here we tested if inoculation with a mixture of arbuscular mycorrhizal fungi (AMF) and/or two strains of plant growth-promoting bacteria (PGPB), in conditions of reduced chemical inpu...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-014-0599-y
更新日期：2015-04-01 00:00:00
abstract：:Nitrogen (N) and phosphorus (P) deposition are increasing worldwide largely due to increased fertilizer use and fossil fuel combustion. Most work with N and P deposition in natural ecosystems has focused on temperate, highly industrialized, regions. Tropical regions are becoming more developed, releasing large amounts...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-016-0749-5
更新日期：2017-05-01 00:00:00
abstract：:Soybean plants can form tripartite symbiotic associations with rhizobia and arbuscular mycorrhizal (AM) fungi, but little is known about effects of co-inoculation with rhizobia and AM fungi on plant growth, or their relationships to root architecture as well as nitrogen (N) and phosphorus (P) availability. In the pres...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-010-0319-1
更新日期：2011-04-01 00:00:00
abstract：:Five caesalpinioid legumes, Afzelia africana, Afzelia bella, Anthonotha macrophylla, Cryptosepalum tetraphylum and Paramacrolobium coeruleum, and one Euphorbiaceae species, Uapaca somon, with a considerable range in seed sizes, exhibited different responses to inoculation by four species of ectomycorrhizal (ECM) fungi...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-005-0007-8
更新日期：2005-12-01 00:00:00
abstract：:Arbuscular mycorrhizal (AM) fungi experience oxidative stress during the plant-fungal interaction, due to endogenous reactive oxygen species (ROS) produced by fungal metabolism and exogenous ROS produced by plant cells. Here, we examine the responses to H2O2 in Gigaspora margarita, an AM fungus containing the endobact...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-017-0790-z
更新日期：2017-11-01 00:00:00
abstract：:Many different cultivation techniques and inoculum products of the plant-beneficial arbuscular mycorrhizal (AM) fungi have been developed in the last decades. Soil- and substrate-based production techniques as well as substrate-free culture techniques (hydroponics and aeroponics) and in vitro cultivation methods have ...
journal_title：Mycorrhiza
pub_type： 杂志文章,评审
doi：10.1007/s00572-010-0337-z
更新日期：2011-01-01 00:00:00
abstract：:It has been shown in a number of pathosystems that arbuscular mycorrhizal (AM) fungi confer resistance against root pathogens, including in interactions between Medicago truncatula and the root rot-causing oomycete Aphanomyces euteiches. For the current study of these interactions, a split root system was established ...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-013-0553-4
更新日期：2014-08-01 00:00:00
abstract：:The effect of arbuscular mycorrhizal (AM) fungi inoculation on pepper (Capsicum annuum L. cv. Zhongjiao 105) plant growth and on some physiological parameters in response to increasing soil Cu concentrations was studied. Treatments consisted of inoculation or not with Glomus mosseae and the addition of Cu to soil at t...
journal_title：Mycorrhiza
pub_type： 杂志文章,收录出版
doi：10.1007/s00572-010-0360-0
更新日期：2011-08-01 00:00:00
abstract：:Inorganic phosphorus (Pi) is essential for plant growth, and phosphate (P) deficiency is a primary limiting factor in Pinus tabulaeformis development in northern China. P acquisition in mycorrhizal plants is highly dependent on the activities of phosphate transporters of their root-associated fungi. In the current stu...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-016-0702-7
更新日期：2016-10-01 00:00:00
abstract：:Mangroves are climax formation of hydrohalophytes inhabiting estuarine or marine salt marshes in the tropics and subtropics. As a terrestrial plant community inhabiting tidally inundated estuarine or marine sediments, mangroves show considerable adaptation to salinity, water-logging and nutrient stress. Thirty-one spe...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-002-0164-y
更新日期：2002-08-01 00:00:00
abstract：:Ectomycorrhizal fungi have been reported to increase root hydraulic conductivity (L pr) by altering apoplastic and plasma membrane intrinsic protein (PIP)-mediated cell-to-cell water transport pathways in associated roots, or to have little effect on root water transport, depending on the interacting species and impos...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-016-0681-8
更新日期：2016-07-01 00:00:00
abstract：:The genus Cistus comprises a group of about 20 shrub species found in wide areas throughout the whole Mediterranean region to the Caucasus. Being one of the main constituents of the Mediterranean-type maquis, this plant genus is peculiar in that it has developed a range of specific adaptations to resist summer drought...
journal_title：Mycorrhiza
pub_type： 杂志文章,评审
doi：10.1007/s00572-006-0047-8
更新日期：2006-09-01 00:00:00
abstract：:Orchid mycorrhizal (OrM) fungi play a crucial role in the ontogeny of orchids, yet little is known about how the structure of OrM fungal communities varies with space and environmental factors. Previous studies suggest that within orchid patches, the distance to adult orchids may affect the abundance of OrM fungi. Man...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-016-0755-7
更新日期：2017-05-01 00:00:00
abstract：:Mycorrhizal fungi are very diverse, including those that produce truffle-like fruiting bodies. Truffle-like fungi are hypogeous and sequestrate (produced below-ground, with an enclosed hymenophore) and rely on animal consumption, mainly by mammals, for spore dispersal. This dependence links mycophagous mammals to myco...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-019-00886-2
更新日期：2019-05-01 00:00:00
abstract：:The aims of this study were to test the effects of a mycorrhiza helper bacterium (MHB), Pseudomonas monteilii strain HR13 on the mycorrhization of (1) an Australian Acacia, A. holosericea, by several ectomycorrhizal fungi or one endomycorrhizal fungus Glomus intraradices, and (2) several Australian Acacia species by P...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-002-0204-7
更新日期：2003-04-01 00:00:00
abstract：:Polyphenol oxidases are known to be produced by a range of ectomycorrhizal (ECM) and ericoid mycorrhizal fungi. These enzymes include laccase (EC 1.10.3.2), catechol oxidase (EC 1.10.3.1) and tyrosinase (EC 1.14.18.1), between which there exists considerable overlap in substrate affinities. In this review we consider ...
journal_title：Mycorrhiza
pub_type： 杂志文章,评审
doi：10.1007/s00572-002-0162-0
更新日期：2002-06-01 00:00:00
abstract：:Mycorrhizal and non-mycorrhizal (NM) maize plants were grown for 4 or 7 weeks in an autoclaved quartz sand-soil mix. Half of the NM plants were supplied with soluble P (NM-HP) while the other half (NM-LP), like the mycorrhizal plants, received poorly soluble Fe and Al phosphate. The mycorrhizal plants were inoculated ...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-001-0136-7
更新日期：2001-12-01 00:00:00
abstract：:This study aimed to elucidate the relationship between ericaceous understorey shrubs and the diversity and abundance of ectomycorrhizal fungi (EcMF) associated with the invasive Pinus strobus and native Pinus sylvestris. Seedlings of both pines were grown in mesocosms and subjected to three treatments simulating diffe...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-010-0350-2
更新日期：2011-07-01 00:00:00
abstract：:The impact of rest grazing on arbuscular mycorrhizal fungi (AMF) and the interactions of AMF with vegetation and soil parameters under rest grazing condition were investigated between spring and late summer in a desert steppe ecosystem with different grazing managements (rest grazing with different lengths of resting ...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-012-0468-5
更新日期：2013-05-01 00:00:00
abstract：:The International Culture Collection of (Vesicular-) Arbuscular Mycorrhizal Fungi-INVAM-the largest living culture collection of arbuscular mycorrhizal fungi (AMF) celebrated its 35th year in 2020. The authors record here the mission and goals of INVAM, its contribution as a living culture collection, some historical ...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-020-01008-z
更新日期：2021-01-01 00:00:00
abstract：:There is growing evidence that the application of biocontrol organisms (e.g., Pseudomonas and Bacillus spp., arbuscular mycorrhizal fungi-AMF) is a feasible option to reduce incidence of plant pathogens in an integrated control strategy. However, the utilization of these microorganisms, in particular AMF, may be threa...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-014-0610-7
更新日期：2015-05-01 00:00:00
abstract：:Rubber tree (Hevea brasiliensis) is of major economic importance in Southeast Asia and for small land holders in Thailand in particular. Due to the high value of latex, plantations are expanding into unsuitable areas, such as the northeast province of Thailand where soil fertility is very low and therefore appropriate...
journal_title：Mycorrhiza
pub_type： 杂志文章
doi：10.1007/s00572-016-0720-5
更新日期：2016-11-01 00:00:00
