Abstract:
OBJECTIVE:To explore the apoptotic role of amylin on human mesangial cell (MC). MATERIALS AND METHODS:Primarily cultured human MCs were applied and treated with fresh amylin preparation. Human MCs were identified by the morphology and immunofluorescence staining. The apoptotic cells were determined by ultrastructure changes, TUNEL, and DNA fragmentation analysis. Propidium iodide staining and flow cytometry was employed for quantitative measurement of apoptosis. RESULTS:Under the light and transmission electronic microscopy (TEM), the human MCs with condensed chromatin, plasma shrinkage, marginated nuclear chromatin or apoptotic body were observed in amylin-treated MCs. Positive TUNEL staining, hypolipoid DNA peak, and typical DNA "ladder" pattern were also detected in amylin-treated MCs. Quantitative analysis of the apoptotic MCs showed that human amylin induced an increase of the percentage of apoptotic cells in a dose-dependent manner. Amylin nano-scale fibrils (5-18nm) in diameter were detected in the cultured solution using negative staining under the TEM. Compared to the control, no significant changes of lactate dehydrogenase release were observed in amylin-treated MCs (P>0.05). CONCLUSIONS:Fibrillogenic amylin evokes the apoptosis of human MCs in vitro, which may explain the mechanism of the hypocellular mesangial damage and progressive glomerulosclerosis of the patients with diabetic nephropathy.
journal_name
Diabetes Res Clin Practjournal_title
Diabetes research and clinical practiceauthors
Peng A,Liu ZH,Zhou H,Zhu MY,Li LSdoi
10.1016/j.diabres.2007.02.018subject
Has Abstractpub_date
2007-10-01 00:00:00pages
16-22issue
1eissn
0168-8227issn
1872-8227pii
S0168-8227(07)00123-4journal_volume
78pub_type
杂志文章abstract:AIMS:Gestational diabetes mellitus (GDM) is associated with adverse obstetrical outcomes and postpartum pre-diabetes/diabetes. As the American Diabetes Association (ADA) criteria for GDM may capture obstetrical risk better than the National Diabetes Data Group (NDDG) criteria, we compared these criteria in their detect...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2009.04.008
更新日期:2009-07-01 00:00:00
abstract::We investigated the preventive effects of ferulic acid (FA) and alpha-tocopherol (AT) on the progression of diabetic nephropathy. Otsuka Long-Evans Tokushima Fatty (OLETF) and Long-Evans Tokushima Otsuka (LETO) rats were used as type 2 diabetes and non-diabetes models, respectively. Two-thirds of the OLETF rats were f...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2007.08.009
更新日期:2008-01-01 00:00:00
abstract::Mortality, macroangiopathic events and end-stage renal disease (ESRD) in the elderly under long-term, intensive multifactorial diabetes control were prospectively investigated. Three hundred and eighty-eight elderly patients (> or =65 years) with type 2 diabetes (the mean age 72.9 years, men/women ratio 176/212) were ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,多中心研究
doi:10.1016/j.diabres.2007.02.019
更新日期:2007-10-01 00:00:00
abstract:AIMS:Accelerated atherosclerosis is the major cause of mortality in diabetic patients and increased oxidative stress probably plays an important role in its development. The aim of our study was to evaluate the relationship between thioredoxin-interacting protein (TXNIP) as an oxidative stress parameter and carotid art...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2015.05.028
更新日期:2015-08-01 00:00:00
abstract::This study was aimed to characterize the vascular production of superoxide in Otsuka Long-Evans Tokushima Fatty (OLETF) rat, a model of type 2 diabetes. The nitroblue tetrazolium staining in the aorta from old (30 weeks) OLETF rat was more prominent than that of age-matched control (LETO) rat, which was significantly ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(02)00278-4
更新日期:2003-04-01 00:00:00
abstract::Prediabetes (IPD; n=122) and normoglycemic individuals (n=100) underwent assessment of polymorphisms of TNFα (-238, -308) and IL6 (-174). After 27.25±5.64 months, 16 IPD had reverted to normoglycemia and 18 progressed to diabetes. TNFα -238AA/GA genotypes were significantly more common in IPD, had higher TNFα, higher ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2012.12.007
更新日期:2013-03-01 00:00:00
abstract:AIMS:Chronic inflammation is linked to type 2 diabetes (T2DM), so we investigated correlations between obesity, blood glucose levels, and inflammation in T2DM patients. METHODS:Peripheral blood mononuclear cells (PBMCs) were collected from 40 T2DM patients (27 men, 13 women; mean age 49.63 years), and 10 non-diabetic ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2010.06.016
更新日期:2010-10-01 00:00:00
abstract::Potassium (K) channels regulate cellular excitability. Their opening hyperpolarises the membrane potential and induces quiescence whereas their closure produces depolarisation and excitation. One K-channel superfamily includes the delayed rectifier (KV), the A-type (KA) and the large conductance, Ca-sensitive (BKCa) c...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,评审
doi:10.1016/0168-8227(95)01080-w
更新日期:1995-08-01 00:00:00
abstract::The role of post-prandial glucose on cardiovascular risk among Chinese adults without diabetes was investigated. In a median follow-up of 3.5 years on 16,590 participants, 95 cardiovascular deaths were found. The relative risk in the highest quintile post-prandial glucose was 1.61 (P for trend, 0.05) for cardiovascula...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2008.11.023
更新日期:2009-02-01 00:00:00
abstract::The predictive value of hemoglobin A1c (HbA1c) in comparison to fasting plasma glucose (FPG) is evaluated for 5-year incident diabetes (DM), as HbA1c may be more practical than FPG in the screening for DM in the future. Of 1189 non-DM subjects aged 35-89 years old from the Funagata Study, 57 subjects (4.8%) had develo...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2009.11.001
更新日期:2010-01-01 00:00:00
abstract::The effect of short-term insulin treatment with a long-acting insulin preparation, Ultratard HM (U-HM) was investigated in eight non-insulin-dependent diabetes mellitus (NIDDM) patients whose previous glycemic control using diet was judged unsatisfactory. The treatment consisted of one daily pre-breakfast injection of...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(87)80058-x
更新日期:1987-11-01 00:00:00
abstract:AIM:This study aimed to evaluate the change of serum levels of proinflammatory molecules in patients with type 2 diabetes and clarify the involvement of these molecules in diabetic nephropathy and atherosclerosis. METHODS:Sixty-six Japanese type 2 diabetic patients (T2DM) and 39 healthy control subjects were enrolled....
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2010.01.012
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of specialized young adult diabetes clinic (YAC) on glycemic control in a young adult patients with type 1 diabetes (DM1) transitioning from pediatric to adult diabetes care. RESEARCH DESIGN AND METHODS:HbA1c was retrospectively analyzed through 3 years in 15-25 y/o DM1 patients enteri...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2007.04.014
更新日期:2007-12-01 00:00:00
abstract:AIMS:TNF-α is a diabetogenic cytokine associated with adverse outcomes during pregnancy that can be counterbalanced by IL-10. We have investigated IL-10 and TNF-α balance at maternal and placental levels in hyperglycemia-associated pregnancies. METHODS:One hundred and ninety-two pregnant women participated, which incl...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2015.01.005
更新日期:2015-03-01 00:00:00
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journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/0168-8227(92)90018-m
更新日期:1992-02-01 00:00:00
abstract:AIMS:We investigated the relationship of urinary albumin excretion (UAE) to metabolic syndrome and type 2 diabetes in Asian. METHODS:A total of 446 subjects (187 without diabetes and 259 with type 2 diabetes) were enrolled in this study. The components of metabolic syndrome, high sensitivity C-reactive protein (hs-CRP...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2008.05.010
更新日期:2008-09-01 00:00:00
abstract:OBJECTIVE:The prevalence of treatment resistant hypertension (RH) depends on methods used for blood pressure (BP) measurements, goals of BP, and therapeutic efforts in terms of medication and adherence. We focused on diabetic subjects and explored the prevalence of RH in primary care practice. METHODS:In 1737 subjects...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2015.08.007
更新日期:2015-10-01 00:00:00
abstract::Previous studies have established that impaired glucose tolerance (IGT) patients with fasting hyperglycemia (IGT/FH: fasting plasma glucose (FPG) level 6.1-7.0 mmol/l and 2 h PG level of 7.8-11.1 mmol/l) exhibit higher insulin resistance than those with isolated IGT (FPG level <6.1 mmol/l and 2 h PG level of 7.8-11.1 ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2003.11.008
更新日期:2004-06-01 00:00:00
abstract::Autophagy is a major cellular clearance mechanism that maintains cellular survival and homeostasis. Autophagy has a crucial role in the progression of diabetes and kidney diseases. AIMS:To investigate serum concentrations of Beclin-1, a key regulator of autophagy, in patients with diabetic kidney disease (DKD). METH...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2018.06.022
更新日期:2018-09-01 00:00:00
abstract:AIM:The use of metformin has been associated with diffusely increased colonic (18)F-fluorodeoxyglucose ((18)F-FDG) uptake. Interestingly, metformin use is associated with moderate weight loss. It could be hypothesized that increased colonic glucose disposal is related to this weight loss. It is unknown whether other fa...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2016.02.009
更新日期:2016-04-01 00:00:00
abstract:AIMS:To analyse time trends of antihyperglycaemic therapy and glycaemic control in adult subjects with type 1, or type 2 diabetes between 2002 and 2014 in Germany/Austria. METHODS:184,864 adults with diabetes (35,144 type 1 diabetes (T1D), 149,720 type 2 diabetes (T2D)) from the DPV-database documented between 2002 an...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2016.03.008
更新日期:2016-05-01 00:00:00
abstract::Increased plasma plasminogen activator inhibitor type 1 (PAI-1), coagulation factor VII (FVII) and fibrinogen levels have been recognized as risk factors for cardiovascular disease. Because a substantially high incidence of cardiovascular disease has been reported in diabetic patients with nephropathy compared with th...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/s0168-8227(97)01384-3
更新日期:1997-04-01 00:00:00
abstract::The present study aimed to reveal racial differences in the metabolic pattern of C-peptide and non-esterified fatty acids (NEFA), and in their associations with cardiovascular measures in healthy urban African (102) and Caucasian women (115) from South Africa. An oral glucose tolerance test was performed with measurem...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2006.10.010
更新日期:2007-07-01 00:00:00
abstract:AIMS:To estimate the real-world effects of offering a group-based lifestyle intervention (GLI) to adults with diabetes. METHODS:This randomized encouragement trial included adult primary care patients in metropolitan Chicago with type 2 diabetes and body mass index ≥24 kg/m2. Participants were randomized to standard c...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.diabres.2018.03.030
更新日期:2018-06-01 00:00:00
abstract:AIMS:To estimate and compare the prescription costs for the management of patients with diabetes over a period of 20 years in Greece, based on real world data. METHODS:The records of outpatients with T2D, monitored at three diabetes centres, were examined in four cross-sections (1998, 2006, 2012, 2018). Prescribed med...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2020.108095
更新日期:2020-04-01 00:00:00
abstract::The aim of our study was to evaluate the efficiency of an individual intensive educational control program on improving the metabolic control of insulin-dependent diabetic patients at short- and long-term follow-up. Fifteen insulin-dependent diabetic subjects with poor metabolic control (hemoglobin A1c > 9%) were incl...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/0168-8227(95)01041-b
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:The relation between diabetes mellitus (DM) and mortality among patients with acute myocardial infarction is still controversial. We evaluated the influence of DM on the in-hospital mortality of acute myocardial infarction (AMI) patients using data from the Tokai Acute Myocardial Infarction Study-II, a multi...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,多中心研究
doi:10.1016/j.diabres.2006.04.011
更新日期:2007-01-01 00:00:00
abstract::Antioxidants probably play an important role in the etiology of type 2 diabetes (DM2). This study evaluated the effects of supplementation with lipoic acid (LA) and α-tocopherol on the lipid profile and insulin sensitivity of DM2 patients. A randomized, double-blind, placebo-controlled trial involving 102 DM2 patients...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.diabres.2011.02.010
更新日期:2011-05-01 00:00:00
abstract::Epidemiologic studies of migrant populations provide very promising clues towards understanding the roles of genetics and environmental factors in the etiology of diabetes mellitus. Populations of Japanese ancestry are of particular interest due to marked differences in prevalence rates of non-insulin dependent diabet...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::A new insulin sensitivity index was devised on the basis of an autoregressive model and its validity was investigated. Using data from the 75-g oral glucose tolerance test (OGTT), 115 subjects were divided into 3 groups: 40 with normal glucose tolerance, 34 with impaired glucose tolerance, and 41 with type 2 diabetes ...
journal_title:Diabetes research and clinical practice
pub_type: 杂志文章
doi:10.1016/j.diabres.2007.05.005
更新日期:2008-01-01 00:00:00