Abstract:
:Multicellular tumour spheroids are cellular aggregates that can be prepared from many types of tumour cells. These three-dimensional structures provide a model for analysing the effects of cell-cell contact and intercellular microenvironments on phenomena such as autocrine regulation of growth factor synthesis. Autoregulation of the synthesis of transforming growth factor-alpha (TGF-alpha) was investigated at the message and protein levels in spheroid and monolayer cultures prepared from the A431 human squamous carcinoma cell line. The epidermal growth factor receptor (EGF-R) of these monolayer A431 cells had an average surface density of 2.2 x 10(6)/cell. Constitutive expression of TGF-alpha mRNA was an average of 3-fold greater in A431 spheroids than in monolayers, even for densely packed, confluent monolayers. This effect did not depend on hypoxic stress within the spheroids. TGF-alpha protein synthesis was enhanced in comparison with that in monolayer culture, reaching a value of up to 2-fold greater on a per cell basis. These results are discussed in the context of a TGF-alpha/EGF-R autocrine loop operating within cells that produce high local concentrations of TGF-alpha in the three-dimensional architecture of a spheroid.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Laderoute KR,Murphy BJ,Short SM,Grant TD,Knapp AM,Sutherland RMdoi
10.1038/bjc.1992.34subject
Has Abstractpub_date
1992-02-01 00:00:00pages
157-62issue
2eissn
0007-0920issn
1532-1827journal_volume
65pub_type
杂志文章abstract::Two hundred patients with node positive stage II breast cancer were randomised to four groups after radical mastectomy and axillary evacuation: (1) Postoperative radiotherapy, (2) Adjuvant chemotherapy with eight courses of CAFt (cyclophosphamide 500 mg m-2 + doxorubicin 40 mg/m-2 + ftorafur 20 mg kg-1 orally day 1-14...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1038/bjc.1992.430
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND:After radical prostatectomy (RP) for prostate cancer (PC), p53 alterations predict biochemical relapse (BCR), however, recent evidence suggests that metastatic relapse (MR) not BCR is a surrogate for PC specific mortality (PCSM). This updated analysis of a previously published study investigated the associat...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0549-8
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Tumour stromal cells differ from its normal counterpart. We have shown that tumour endothelial cells (TECs) isolated from tumour tissues are also abnormal. Furthermore, we found that mRNAs of vascular endothelial growth factor-A (VEGF-A) and cyclooxygenase-2 (COX-2) were upregulated in TECs. Vascular endothe...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.20
更新日期:2011-03-01 00:00:00
abstract::Resistance to cytotoxic agents may be encountered during the treatment of acute myeloblastic leukaemia (AML). P-glycoprotein encoded by the MDR-1 gene has been implicated as a potential drug resistance mechanism in leukaemic cells. In recent years, many data have been accrued concerning the expression of P-glycoprotei...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/bjc.1994.70
更新日期:1994-02-01 00:00:00
abstract::Neurofilaments (NFs) are specific intermediate filaments to neural cells. Mammalian NFs are protein triplets composed of three major subunits with respective molecular weights of approximately 70, 150 and 200 kD. Using an immunohistochemical method, 13 carcinoid tumours from different sites were examined for the prese...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.161
更新日期:1991-05-01 00:00:00
abstract::Fifteen human glioma cell lines were examined for their sensitivity to 1,3-bis(chloroethyl)-nitrosourea (BCNU, carmustine) and cis-dichlorodiamminoplatinum (cisplatin), the induction of DNA interstrand cross-linking (DNA-ISC) induced by the two agents and cellular O6-alkylguanine alkyltransferase (ATase) activity. Cel...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.87
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Tumours are responsive to temozolomide (TMZ) if they are deficient in O(6)-methylguanine-DNA methyltransferase (MGMT), and mismatch repair (MMR) proficient. METHODS:The effect of TMZ on medulloblastoma (MB) cell killing was analysed with clonogenic survival assays. Expression of DNA repair genes and enzymes...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.403
更新日期:2012-10-09 00:00:00
abstract:BACKGROUND:Tumours consist of heterogeneous cancer cells and are likely to contain drug-tolerant cell subpopulations, causing early relapse. However, treatment strategies to eliminate these cells have not been established. METHODS:We established gastric cancer patient-derived cells (PDCs) to examine the contribution o...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-019-0600-9
更新日期:2019-11-01 00:00:00
abstract::The cytotoxic mechanism of a conjugate of doxorubicin (DXR) and glutathione (GSH) via glutaraldehyde (GSH-DXR) was investigated using DXR-sensitive (AH66P) and -resistant (AH66DR) rat hepatoma cells. GSH-DXR accumulated in AH66DR cells as well as in AH66P cells without efflux by P-gp and exhibited the potent cytocidal...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.557
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Activated anaplastic lymphoma kinase (ALK) gene fusions are recurrent events in a small fraction of colorectal cancers (CRCs), although these events have not yet been exploited as in other malignancies. METHODS:We detected ALK protein expression by immunohistochemistry and gene rearrangements by fluorescenc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.401
更新日期:2015-12-22 00:00:00
abstract::The association between soya foods and breast cancer risk was investigated in a prospective study of 34759 women in Hiroshima and Nagasaki, Japan. Women completed dietary questionnaires in 1969-1970 and/or in 1979-1981 and were followed for incident breast cancer until 1993. The analysis involved 427 cases of primary ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6690837
更新日期:1999-12-01 00:00:00
abstract::We have recently established a cancer-reactive human monoclonal antibody, GAH, with a positive ratio of over 90% against stomach cancer. GAH was formulated as polyethyleneglycol (PEG)-modified immunoliposomal doxorubicin (DXR) (ILD) and its efficacy was examined against gastrointestinal human cancers. In in vitro stud...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6601341
更新日期:2003-10-20 00:00:00
abstract::We evaluated whether social deprivation affected decision-making for breast cancer surgery. Of 3419 patients, 53.6% had mastectomy and this was predicted by deprivation, age, tumour size and hospital, all of which retained significance on multivariate analysis, except deprivation. Pathological characteristics and surg...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602390
更新日期:2005-02-28 00:00:00
abstract::This study reports on the frequency and disease association pattern of a number of gene rearrangements in a large panel of lymphoid tumours (n = 94). We detected the t(11;14) translocation, involving rearrangement of the BCL-1 locus, in 60% of mantle cell lymphomas. The BCL-2 gene, located at band 18q21, was rearrange...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.279
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:The interaction between human prostate cancer (PCa) cells and bone marrow (BM) endothelium follows a rolling-and-adhesion cascade mediated by E-selectin ligand (ESL): E-selectin. This adhesion is enabled by elevated expression of α-1,3-fucosyltransferases (FTs), enzymes responsible for ESL-mediated bone meta...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.690
更新日期:2013-12-10 00:00:00
abstract::We screened 145 HIV-infected non-pregnant women at a tertiary care centre in Lusaka, Zambia. Liquid-based cytology and human papillomavirus (HPV) genotyping with PGMY09/11 biotinylated primers (Roche Linear Array HPV genotyping test) maximised sensitivity of cytology and HPV assessments. Among high-risk (HR) types, HP...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603737
更新日期:2007-05-07 00:00:00
abstract:BACKGROUND:The role of further hormone therapy in castration-resistant prostate cancer (CRPC) remains unclear. We performed a multi-centre randomised phase III study comparing the use of Dexamethasone, Aspirin, and immediate addition of Diethylstilbestrol (DAiS) vs Dexamethasone, Aspirin, and deferred (until disease pr...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/bjc.2011.7
更新日期:2011-02-15 00:00:00
abstract::Little is known about chemoradiotherapy (CRT) in elderly patients with a locally advanced oesophageal cancer (OC). The aim of our study was to evaluate the tolerance and the outcome of elderly patients older than 70 years treated with CRT for a non-metastatic OC. Chemoradiotherapy was based on radiotherapy combined wi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604749
更新日期:2008-11-18 00:00:00
abstract::We analysed two case-control studies of women from Italy and Switzerland, including 195 cases of oral and pharyngeal cancers and 1113 controls. The multivariate odds ratio was 4.6 for heavy smokers and 2.7 for high alcohol intake. Vegetables, fruit, beta-carotene and wholegrain foods were inversely, butter and retinol...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.1999.0900
更新日期:2000-01-01 00:00:00
abstract::Three biochemical markers of collagen metabolism were measured in 39 osteosarcoma patients. The pretreatment values did not predict outcome, and the markers showed no consistent change upon development of metastases. Both the age of the patients and the multimodality therapy affected the collagen metabolites. These fi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.19
更新日期:1996-01-01 00:00:00
abstract::A cell-free plasmid reactivation assay was used to determine the fidelity of DNA double-strand break (DSB) repair in a panel of eight DSB repair-proficient human tumour cell lines. Nuclear protein extracts derived from radiosensitive tumour cells were less capable of correctly rejoining EcoRI-induced DSBs than were si...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.576
更新日期:1997-01-01 00:00:00
abstract::BGC9331 is a rationally designed, specific nonpolyglutamatable thymidylate synthase (TS) inhibitor that is active in gynaecological malignancies. In the light of the sensitivity of human ovarian tumour cell lines to BGC9331 and non-cross resistance to platinum drugs, we studied the combination BGC9331/carboplatin (BCA...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/sj.bjc.6602811
更新日期:2005-10-17 00:00:00
abstract::All hospital-treated cases of Burkitt's lymphoma (BL), with onset of symptoms in the period 1963-68 and resident in the Lango and Acholi districts of Uganda, were identified. The average annual incidence of BL in the 6-year period was 1-87 X 10(-5), similar to that in the adjacent West Nile district. Contrary to findi...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1977.102
更新日期:1977-05-01 00:00:00
abstract::Perforin (PFP) is a cytotoxic protein released from killer cells. PFP immunoreactivity in human peripheral blood lymphocytes (PBL) and tumour infiltrating lymphocytes (TIL) was investigated immunocytochemically with the aid of an anti-PFP monoclonal antibody. PFP was detected in the cytoplasm of 10% of PBL. We perform...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1991.283
更新日期:1991-08-01 00:00:00
abstract::Helicobacter has been suggested to play a possible role in hepatitis, gallstones, and hepatobiliary tumours. We assessed whether seropositivity to 15 H. pylori proteins was associated with subsequent incidence of 74 biliary tract and 105 liver cancer cases vs. 357 matched controls in the Prostate, Lung, Colorectal, an...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-0961-0
更新日期:2020-09-01 00:00:00
abstract::Increasingly, treatment choices leading to the same survival outcome can be offered to cancer patients (e.g. mastectomy or conservative surgery in early breast cancer). Two approaches available for post-orchidectomy, stage I patients with non-seminomatous germ cell tumours of the testis (NSGCTT), particularly those at...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.570
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:High amplification of epiregulin (EREG) and amphireglin (AREG) in tumour tissues has been previously reported to be associated with better outcome in metastatic colorectal cancer (mCRC) patients who were treated with anti-EGFR antibodies. Here we investigated associations between the expression of other cand...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2016.74
更新日期:2016-04-26 00:00:00
abstract:BACKGROUND:Tumours are made up of a mixed population of different types of cells that include normal structures as well as ones associated with the malignancy, and there are multiple interactions between the malignant cells and the local microenvironment. These intercellular interactions, modulated by the microenvironm...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.517
更新日期:2012-01-03 00:00:00
abstract:BACKGROUND:Magnetic resonance imaging (MRI) has been proposed to guide breast cancer surgery by measuring residual tumour after neoadjuvant chemotherapy. This study-level meta-analysis examines MRI's agreement with pathology, compares MRI with alternative tests and investigates consistency between different measures of...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1038/bjc.2013.473
更新日期:2013-09-17 00:00:00
abstract::An increasing body of evidence indicates that glutathione S-transferases play a role in the intrinsic and acquired resistance of tumours to anticancer drugs. In view of the wide use of tumour cell lines to understand the factors which confer either sensitivity or resistance to chemotherapeutic agents we have determine...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.280
更新日期:1989-09-01 00:00:00