Abstract：
:The aim of the present study was to elucidate the effects of granulocyte colony-stimulating factor (G-CSF)-mediated mobilization of bone marrow-derived stem cells on the injured spinal cord. Bone marrow cells of green fluorescent protein (GFP) transgenic mice were transplanted into lethally irradiated C57BL/6 mice. Four weeks after bone marrow transplantation, spinal cord injury was produced by a static load (20 g, 5 min) at T8 level. G-CSF (200 microg/kg/day) was injected subcutaneously for 5 days. Immunohistochemistry for GFP and cell lineage markers was performed to evaluate G-CSF-mediated mobilization of bone marrow-derived cells into injured spinal cord. Hind limb locomotor recovery was assessed for 6 weeks. Immunohistochemistry revealed that G-CSF increased the number of GFP-positive cells in injured spinal cord, indicating that bone marrow-derived cells were mobilized and migrated into injured spinal cord. The numbers of double positive cells for GFP and glial markers were larger in the G-CSF treated mice than in the control mice. Luxol Fast Blue staining revealed that G-CSF promoted white matter sparing. G-CSF treated mice showed significant recovery of hind limb function compared to that of the control mice. In conclusion, G-CSF showed efficacy for spinal cord injury treatment through mobilization of bone marrow-derived cells.
journal_name
Brain Resjournal_title
Brain researchauthors
Koda M,Nishio Y,Kamada T,Someya Y,Okawa A,Mori C,Yoshinaga K,Okada S,Moriya H,Yamazaki Mdoi
10.1016/j.brainres.2007.02.058subject
Has Abstractpub_date
2007-05-29 00:00:00pages
223-31eissn
0006-8993issn
1872-6240pii
S0006-8993(07)00482-9journal_volume
1149pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The enzyme in brain that hydrolyzes 2',3'-cyclic nucleotides to the 2'-mononucleotides has been found by several authors to be concentrated in the myelin fraction. To facilitate further study of the enzyme, one of our objectives has been to develop a method of solubilizing the enzyme without the use of detergents. Whe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90719-2
更新日期：1975-05-23 00:00:00
abstract：:The distribution of the regulatory (RII beta) subunits of type II cAMP-dependent protein kinase in cortical and subcortical areas was examined in human control and Alzheimer's disease (AD) brains. Four monoclonal antibodies generated against bovine brain RII, which cross-reacted with human brain RII beta, detected RII...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90230-7
更新日期：1992-04-24 00:00:00
abstract：:Alteration of blood-brain barrier (BBB) permeability by 2450 MHz CW microwaves was assessed semi-quantitatively after intravenous injection of horseradish peroxidase (HRP) and exposure of conscious, unrestrained rats to incident power densities of 0, 20 or 65 mW/cm2 for 30, 90 or 180 min. Additional rats were exposed ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-0173(84)90022-5
更新日期：1984-05-01 00:00:00
abstract：:Microinjection of mRNA from adult rat brains into Xenopus oocytes induced an electrophysiological response to acetylcholine in the cells. The cells responded to acetylcholine through muscarinic receptors which appeared on the cell surface after microinjection of the mRNA and showed the depolarization, mainly due to an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90166-0
更新日期：1985-07-15 00:00:00
abstract：:The present study was designed to investigate the role of activation of Kca+2 channels and cAMP in opioid-induced pial artery dilation in newborn pigs equipped with closed cranial windows. Methionine enkephalin, an endogenous mu agonist, elicited dilation that was modestly attenuated by the Kca+2 channel antagonist, i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01284-x
更新日期：1997-02-07 00:00:00
abstract：:The actions of pentobarbital (PB) and diphenylhydantoin (DPH) have been studied on neurons of dorsal root ganglia from adult rats. At anesthetic level (i.e. 1--2 x 10(-4) M), PB greatly enhanced neuronal responses to gamma-aminobutyric acid (GABA); at 10(-3) M PB caused a small depolarization and profoundly attenuated...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)90370-x
更新日期：1981-03-02 00:00:00
abstract：:To test the hypothesis that stimulation of glial peroxidase activity by estrogens may play a role in the pathogenesis of the previously reported degenerative changes in the hypothalamic arcuate nucleus that occur in female rats treated with a long-acting estrogen preparation, the cellular localization and number of pe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90273-e
更新日期：1990-01-22 00:00:00
abstract：:Because the occurrence of rapid eye movement (REM) sleep is critically dependent upon an animal's thermal balance, acute pharmacological treatments may effect REM sleep through effects on thermoregulation. We describe how manipulating ambient temperature (T(a)) alters the REM sleep-suppressing effects of systemically-...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90184-8
更新日期：1994-02-04 00:00:00
abstract：:Antimitotic drugs used in the chemotherapeutic treatment of cancers induce undesirable but unavoidable side-effects from interruption of normal mitotic processes throughout the body. We have examined whether several such drugs capable of penetrating the blood-brain barrier - thioTEPA and 5-fluorouracil - influence the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.06.093
更新日期：2006-09-21 00:00:00
abstract：INTRODUCTION:Apathy is the most common neuropsychiatric syndrome in behavioral variant frontotemporal dementia (bvFTD), and encompasses cognitive, behavioral and affective symptoms. The neural basis of apathy in bvFTD is not completely understood. Previous neuroimaging studies have poorly considered executive impairmen...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2020.146799
更新日期：2020-06-15 00:00:00
abstract：:Many of our daily movements use visual information to guide our arms toward objects of interest. Typically, these visually guided movements involve first focusing our gaze on the intended target and then reaching toward the direction of our gaze. The literature on eye-hand coordination provides a great deal of evidenc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.05.057
更新日期：2009-08-11 00:00:00
abstract：:The effect of hippocampal kindling on neuronal and glial marker proteins was studied in the rat by immunochemical methods. In hippocampus, pyriform cortex and amygdala there was an increase in glial fibrillary acidic protein (GFAP), indicating reactive gliosis, and an increase in the glycolytic enzyme NSE, suggesting ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90791-9
更新日期：1990-10-29 00:00:00
abstract：:The development of bombesin-like peptides and their receptors was determined in 9 different rat brain regions. The concentration of immunoreactive bombesin increased dramatically in all brain regions examined during the third week after birth. In contrast, the density of [125I-Tyr4]bombesin binding sites was near maxi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(84)90107-x
更新日期：1984-08-01 00:00:00
abstract：:The role of the substantia innominata (SI) in the generation of a behavior mediated by the prefrontal (PF) cortex was examined in two Japanese monkeys. PF neuronal activities related to a visual tracking task by wrist movement were recorded and neural responses to SI stimulation were analyzed. Sixty-six neurons showed...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90734-5
更新日期：1983-10-16 00:00:00
abstract：:In decerebrate rats, the responses of tilt-sensitive neurons in the lateral and descending vestibular nuclei were studied during constant velocity 10 degrees off-vertical axis rotations (OVAR) in the clockwise (VW) and counterclockwise (CCW) directions. Seventy three otolith-related units showed a sinusoidal position-...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00687-l
更新日期：1995-09-25 00:00:00
abstract：:In Parkinson disease (PD), the subthalamic nucleus (STN) becomes hyperactive (disinhibited), which is reported to cause excitotoxic damage to midbrain dopaminergic neurons. Here, we examined whether silencing of the hyperactive STN by chronic bilateral deep brain stimulation (DBS) increased the survival of midbrain do...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.08.082
更新日期：2006-11-20 00:00:00
abstract：OBJECTIVE:This study aimed to investigate whether β-amyloid (Aβ) was able to enhance neurocan expression in a Sox9 dependent manner in astrocytes. METHODS AND MATERIALS:Astrocytes were incubated with Aβ at different concentrations, the expression of Sox9 and neurocan was detected by Western blot assay. Meanwhile, the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.06.010
更新日期：2016-09-01 00:00:00
abstract：:The hindpaw was partially denervated by the tibial nerve transection in adult rats. At post-transection intervals varying from 2 to 168 days, the hindpaw was stimulated bilaterally by subcutaneous injection of formalin. The excitability of dorsal horn neurons was expressed as the percentage ratio of the number of form...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90942-3
更新日期：1994-04-11 00:00:00
abstract：:In anaesthetized cats, medial gastrocnemius motor units (MUs) were stimulated with random sequences (mean rates between 6 and 12 pps) of electrical pulses delivered to their axons in small ventral root filaments. Muscle tension was recorded under isometric conditions, and spike trains of muscle spindle afferents were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91656-2
更新日期：1986-02-19 00:00:00
abstract：:In gerbils pentylenetetrazole- or handling-induced seizures were accompanied by cerebral formation of small amounts of cysteinyl-leukotrienes (LT) but large amounts of prostaglandin (PG) F2 alpha. By contrast, in rats injected with pentylenetetrazole or bicuculline very large amounts of PGF2 alpha but no cysteinyl-LT ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)90519-2
更新日期：1991-02-01 00:00:00
abstract：:The localization of prostaglandin endoperoxide synthase in monkey brain was investigated by the immunoperoxidase method using the monoclonal antibody (PES-7) raised against the enzyme purified from bovine seminal vesicle. The frozen sections with 30-microns thickness were employed after the brain was fixed with perfus...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91043-z
更新日期：1991-03-08 00:00:00
abstract：:The blink reflex consists of an early, pontine R1-component and a late, medullary R2-component. R1 and R2 can be evoked by innocuous stimuli, but only the R2 also by painful heat, suggesting that the R2 is mediated by wide dynamic range neurons (WDR) of the spinal trigeminal nucleus. Remote noxious stimuli suppress th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00646-5
更新日期：1998-08-24 00:00:00
abstract：:An interaction between angiotensin II (Ang II) receptors and alpha 2-adrenoceptors was evaluated in the nucleus tractus solitarii (NTS) of the normotensive Wistar-Kyoto rat (WKY) and of the spontaneously hypertensive rat (SHR) using quantitative receptor autoradiography and cardiovascular analysis. In the WKY rat, Ang...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00220-k
更新日期：1995-05-08 00:00:00
abstract：:We have previously raised a monoclonal antibody (69A1) which recognises a cell-surface antigen expressed on rat cerebellar neurons both in culture and in tissue sections of the developing cerebellum prior to postnatal day 17. In our current study we have undertaken a detailed analysis of the loss of labelling from the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90087-8
更新日期：1986-09-01 00:00:00
abstract：:The impact of perinatal exposure to environmental tobacco smoke (ETS) on cognitive development is controversial. We exposed rhesus monkeys to ETS or filtered air (5 animals per group) beginning in utero on day 50 of pregnancy and continuing throughout postnatal testing. In infancy, we evaluated both groups for visual ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.03.001
更新日期：2007-06-02 00:00:00
abstract：:Metabolites formed from testosterone in the brain, their neuroanatomical distribution and the kinetic properties of the enzymes were studied in male and female Japanese quail chicks and compared with the adult brain using an in vitro radioassay. Oestradiol-17 beta. 5 alpha-dihydrotestosterone, 5 beta-dihydrotestostero...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90149-5
更新日期：1986-02-01 00:00:00
abstract：:In this study, we aimed at investigating the effect of an enriched environment (EE) on the recovery from chronic inflammatory pain. Inflammatory pain was induced by the injection of 2 mg of carrageenan (CAR) into the right knee of male Sprague-Dawley rats (n=34). Rats were housed either singly (S-housed) or in an EE (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.04.039
更新日期：2009-06-18 00:00:00
abstract：:The differential effects of the pyrethroid tetramethrin on tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant (TTX-R) single sodium channel currents in rat dorsal root ganglion (DRG) neurons were investigated using the outside-out configuration of patch-clamp technique. Channel conductances were 10.7 and 6.3 pS...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01449-7
更新日期：1996-03-18 00:00:00
abstract：:Given that relatively little is known regarding the central control of brown adipose tissue (BAT)-mediated thermogenesis the present study assessed whether the direct pharmacological stimulation of beta- or alpha-adrenergic receptors located on the brown adipocytes would result in a typical thermogenic response follow...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91482-g
更新日期：1991-04-19 00:00:00
abstract：:Strychnine-sensitive, inhibitory glycine receptors have not until lately been considered to play a significant role in neurotransmission in mammalian forebrain regions. In order to investigate the role of glycine as a neurotransmitter in brain we have measured glycine induced chloride fluxes in different adult rat for...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01484-5
更新日期：1996-03-11 00:00:00
