Differential effects of bryostatin 1 and phorbol ester on human breast cancer cell lines.

Abstract:

:The effects of the protein kinase C (PKC) activators, phorbol ester 12-O-tetradecanoyl-13-phorbol acetate (TPA) and the marine natural product, bryostatin 1, on the growth and morphology of human breast cancer cell lines were examined. TPA (1 to 100 nM) inhibited growth of four of six cell lines by up to 75% in 5-day cultures. Bryostatin 1 inhibited growth of only MCF-7 cells and only at a high dose (100 nM). However, bryostatin 1 completely antagonized the growth inhibition and morphological changes induced by TPA in MCF-7 cells. The divergent effects of these two agents are associated with differing effects on PKC activity and isoform expression in MCF-7 cells. TPA induced rapid translocation of the PKC-alpha isozyme and PKC activity to the membrane fraction of MCF-7 cells. In contrast, bryostatin 1 treatment resulted in the loss of the PKC-alpha isozyme and PKC activity from both cytosolic and membrane compartments within 10 min of treatment. In coincubation assays the bryostatin 1 effect was dominant over that of TPA. Similar effects on PKC-alpha isozyme and PKC activity were seen in a second cell line whose growth was inhibited by TPA but not by bryostatin 1, MDA-MB-468. In contrast, in the T47D cell line, where TPA was not growth inhibitory, TPA failed to induce translocation of PKC-alpha to the cell membrane. Bryostatin, however, still caused loss of PKC-alpha isozyme and PKC activity from cytosolic and membrane fractions. Thus, differential actions of bryostatin 1 and TPA on PKC activity and alpha-isoform level in the membrane-associated fraction of MCF-7 and MDA-MB-468 cells may account for the divergent effects of these two agents on cell growth and morphology. These results suggest that the PKC-alpha isoform may specifically play a role in inhibiting growth of human breast cancer cells.

journal_name

Cancer Res

journal_title

Cancer research

authors

Kennedy MJ,Prestigiacomo LJ,Tyler G,May WS,Davidson NE

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

1278-83

issue

5

eissn

0008-5472

issn

1538-7445

journal_volume

52

pub_type

杂志文章
  • Intact nitric oxide synthase II gene is required for interferon-beta-mediated suppression of growth and metastasis of pancreatic adenocarcinoma.

    abstract::Previous studies have shown that enforced expression of IFN-beta suppressed tumor growth and metastasis. In this report, we determined whether the induction of nitric oxide synthase II (NOS II) gene is required for IFN-beta-mediated antitumor activity using syngeneic mice with intact (NOS II+/+) or genetically disrupt...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wang B,Xiong Q,Shi Q,Le X,Abbruzzese JL,Xie K

    更新日期:2001-01-01 00:00:00

  • Preparation of synthetic polypeptide domains of carcinoembryonic antigen and their use in epitope mapping.

    abstract::Genes encoding the four principal polypeptide domains (N, A1-B1, A2-B2, and A3-B3) of carcinoembryonic antigen (CEA) were synthesized and expressed in Escherichia coli as fusion products with bacterial CMP-KDO synthetase (CKS). The four synthetic fusion proteins were purified in high yield and used as targets in Weste...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hass GM,Bolling TJ,Kinders RJ,Henslee JG,Mandecki W,Dorwin SA,Shively JE

    更新日期:1991-04-01 00:00:00

  • RSK Regulates PFK-2 Activity to Promote Metabolic Rewiring in Melanoma.

    abstract::Metabolic reprogramming is a hallmark of cancer that includes increased glucose uptake and accelerated aerobic glycolysis. This phenotype is required to fulfill anabolic demands associated with aberrant cell proliferation and is often mediated by oncogenic drivers such as activated BRAF. In this study, we show that th...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-17-2215

    authors: Houles T,Gravel SP,Lavoie G,Shin S,Savall M,Méant A,Grondin B,Gaboury L,Yoon SO,St-Pierre J,Roux PP

    更新日期:2018-05-01 00:00:00

  • Inhibition of proliferation and estrogen receptor signaling by peroxisome proliferator-activated receptor gamma ligands in uterine leiomyoma.

    abstract::Peroxisome proliferator-activated receptor (PPAR) gamma is an important signaling molecule in cells of mesenchymal origin, inducing differentiation and regulating cell proliferation in several cell types such as vascular smooth muscle cells. Leiomyomas arise from smooth muscle cells of the uterine myometrium with an i...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Houston KD,Copland JA,Broaddus RR,Gottardis MM,Fischer SM,Walker CL

    更新日期:2003-03-15 00:00:00

  • In vivo administration of MKT-077 causes partial yet reversible impairment of mitochondrial function.

    abstract::The effects of in vivo administration of a pharmacologically toxic dose of the lipophilic cationic compound, MKT-077, were investigated in selected vital organs of the rat. MKT-077 (15 mg/kg body weight), administered by bolus i.v. injection every day for 5 days, did not detectably influence rat heart and kidney mitoc...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Weisberg EL,Koya K,Modica-Napolitano J,Li Y,Chen LB

    更新日期:1996-02-01 00:00:00

  • Elevated interleukin-6 and G-CSF in human pancreatic cancer cell conditioned medium suppress dendritic cell differentiation and activation.

    abstract::Although dendritic cell (DC) function is impaired in pancreatic cancer patients, the underlying mechanisms are unknown. This study analyzed the soluble factors released by pancreatic cancer cells responsible for inhibiting DC differentiation and activation. Medium conditioned by a highly metastatic human pancreatic ca...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-3963

    authors: Bharadwaj U,Li M,Zhang R,Chen C,Yao Q

    更新日期:2007-06-01 00:00:00

  • Carcinogenicity studies of tobacco extract in vitamin A-deficient Sprague-Dawley rats.

    abstract::Long-term carcinogenicity studies were carried out in male Sprague-Dawley rats maintained on vitamin A-sufficient (SLO+) and vitamin A-deficient (SLO-) diets and treated with tobacco extract (TE). Three-week-old rats received by gavage a total dose of 860 mg of TE at a daily dose of 3 mg/rat over a period of 21 months...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bhide SV,Ammigan N,Nair UJ,Lalitha VS

    更新日期:1991-06-01 00:00:00

  • Turcot's syndrome of glioma and polyposis occurs in the absence of germ line mutations of exons 5 to 9 of the p53 gene.

    abstract::The term "Turcot's syndrome" has been used to describe approximatively 55 patients with an association of colonic polyposis and primary neuroepithelial tumors of the central nervous system. The p53 tumor suppressor gene is a possible candidate underlying the syndrome because (a) mutations in the p53 gene are ubiquitou...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kikuchi T,Rempel SA,Rutz HP,de Tribolet N,Mulligan L,Cavenee WK,Jothy S,Leduy L,Van Meir EG

    更新日期:1993-03-01 00:00:00

  • Synchronization of hepatocellular DNA synthesis in regenerating rat liver by continuous infusion of hydroxyurea.

    abstract::Hydroxyurea (HU) inhibited DNA synthesis in the livers of partially hepatectomized rats. After an i.v. infusion of HU begun in the late G1 phase and continued for periods of up to 30 hr, all hepatocytes scheduled to embark on DNA synthesis in a characteristic intralobular sequence during this time interval accumulated...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Rabes HM,Iseler G,Czichos S,Tuczek HV

    更新日期:1977-04-01 00:00:00

  • Determination of Tumor Margins with Surgical Specimen Mapping Using Near-Infrared Fluorescence.

    abstract::For many solid tumors, surgical resection remains the gold standard and tumor-involved margins are associated with poor clinical outcomes. Near-infrared (NIR) fluorescence imaging using molecular agents has shown promise for in situ imaging during resection. However, for cancers with difficult imaging conditions, surg...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-18-0878

    authors: Gao RW,Teraphongphom NT,van den Berg NS,Martin BA,Oberhelman NJ,Divi V,Kaplan MJ,Hong SS,Lu G,Ertsey R,Tummers WSFJ,Gomez AJ,Holsinger FC,Kong CS,Colevas AD,Warram JM,Rosenthal EL

    更新日期:2018-09-01 00:00:00

  • Mutagenic processing of ethylation damage in mammalian cells: the use of methoxyamine to study apurinic/apyrimidinic site-induced mutagenesis.

    abstract::The aldehyde reagent methoxyamine is able to interact with apurinic/apyrimidinic sites formed in vivo within cells and displays both an anti-cytotoxic and an antimutagenic activity on N-ethyl-N'-nitro-N-nitrosoguanidine-induced DNA damage in Chinese hamster ovary cells. To clarify the underlying mechanism we have exam...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Fortini P,Calcagnile A,Vrieling H,van Zeeland AA,Bignami M,Dogliotti E

    更新日期:1993-03-01 00:00:00

  • Immunization against feline oncarnavirus disease using a killed tumor cell vaccine.

    abstract::Specific pathogen-free cats were immunized with an inactivated feline oncornavirus tumor cell vaccine. Immunized cats produced high antibody titers to the feline oncornavirus-associated cell membrane antigen and were protected from oncogenic feline sarcoma virus challenge. However, immunization did not produce virus-n...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Olsen RG,Hoover EA,Mathes LE,Heding L,Schaller JP

    更新日期:1976-10-01 00:00:00

  • Application of a new approach for the quantitation of drug synergism to the combination of cis-diamminedichloroplatinum and 1-beta-D-arabinofuranosylcytosine.

    abstract::This report describes the application of a new approach, the universal response surface approach, to the quantitative assessment of drug interaction, i.e., the determination of synergism, antagonism, additivity, potentiation, inhibition, and coalitive action. The specific drug combination and experimental growth syste...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Greco WR,Park HS,Rustum YM

    更新日期:1990-09-01 00:00:00

  • Epigenetic silencing of the tumor suppressor microRNA Hsa-miR-124a regulates CDK6 expression and confers a poor prognosis in acute lymphoblastic leukemia.

    abstract::Whereas transcriptional silencing of genes due to epigenetic mechanisms is one of the most important alterations in acute lymphoblastic leukemia (ALL), some recent studies indicate that DNA methylation contributes to down-regulation of miRNAs during tumorigenesis. To explore the epigenetic alterations of miRNAs in ALL...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-4025

    authors: Agirre X,Vilas-Zornoza A,Jiménez-Velasco A,Martin-Subero JI,Cordeu L,Gárate L,San José-Eneriz E,Abizanda G,Rodríguez-Otero P,Fortes P,Rifón J,Bandrés E,Calasanz MJ,Martín V,Heiniger A,Torres A,Siebert R,Román-Gomez J,

    更新日期:2009-05-15 00:00:00

  • Identification of a human homologue of the murine tumor rejection antigen GP96.

    abstract::A family of cell surface glycoproteins with a molecular weight of 96,000 (gp96) has recently been implicated in the individually distinct immunogenicity of chemically induced sarcomas of inbred mice. Rabbit antiserum to murine gp96 detects an antigenically related Mr 96,000 cell surface glycoprotein on two cultured hu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Srivastava PK,Old LJ

    更新日期:1989-03-15 00:00:00

  • Cell-mediated antitumor immunity in breast cancer patients evaluated by antigen-induced leukocyte adherence inhibition in test tubes.

    abstract::Tumor antigen-induced inhibition of leukocyte adherence was adapted and modified for use in glass test tubes for the study of cell-mediated antitumor immunity to human adenocarcinoma of the breast. Peripheral blood leukocytes from 40 to 47 patients with proven breast cancer responded to an antigenic extract of breast ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Grosser N,Thomson DM

    更新日期:1975-09-01 00:00:00

  • Simultaneous inhibition of PDK1/AKT and Fms-like tyrosine kinase 3 signaling by a small-molecule KP372-1 induces mitochondrial dysfunction and apoptosis in acute myelogenous leukemia.

    abstract::Phosphoinositol-3-kinase (PI3K)/protein kinase B (AKT) and Fms-like tyrosine kinase 3 (FLT3) signaling are aberrantly activated in acute myelogenous leukemia (AML) cells. Constitutively activated AKT and FLT3 regulate leukemia cell survival and resistance to chemotherapy. In this study, we investigated the effects of ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-1278

    authors: Zeng Z,Samudio IJ,Zhang W,Estrov Z,Pelicano H,Harris D,Frolova O,Hail N Jr,Chen W,Kornblau SM,Huang P,Lu Y,Mills GB,Andreeff M,Konopleva M

    更新日期:2006-04-01 00:00:00

  • Cytotoxicity of 5,6-dihydroxytryptamine in dimethylhydrazine-induced carcinomas of rat colon.

    abstract::Male Sprague-Dawley rats were given weekly s.c. injections of 1,2-dimethylhydrazine (21 mg/kg) for 20 weeks. The injections were then discontinued, and, after an interval of 2 to 8 weeks, experimental animals were given i.p. injections of 5,6-dihydroxytryptamine (5,6DHT) at a dose of 40 mg/kg and sacrificed at interva...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Tutton PJ,Barkla DH

    更新日期:1977-04-01 00:00:00

  • Telomerase activity in prostate cancer, prostatic intraepithelial neoplasia, and benign prostatic epithelium.

    abstract::Telomerase is a ribonucleoprotein that synthesizes telomeric DNA on chromosomal ends. Telomerase activation has been seen in many immortal cell lines and cancers. Telomerase activity was analyzed in prostate carcinoma; in coexistent prostatic intraepithelial neoplasia (PIN), benign prostatic hyperplasia (BPH), atrophy...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Zhang W,Kapusta LR,Slingerland JM,Klotz LH

    更新日期:1998-02-15 00:00:00

  • Metabolic activation of temozolomide measured in vivo using positron emission tomography.

    abstract::The purpose of this research was to quantitate and confirm the mechanism of in vivo metabolic activation of temozolomide. The secondary aims were to evaluate the tumor, normal tissue, and plasma pharmacokinetics of temozolomide in vivo, and to determine whether such pharmacokinetics resulted in tumor targeting. [(11)C...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Saleem A,Brown GD,Brady F,Aboagye EO,Osman S,Luthra SK,Ranicar AS,Brock CS,Stevens MF,Newlands E,Jones T,Price P

    更新日期:2003-05-15 00:00:00

  • Targeting of PYK2 Synergizes with EGFR Antagonists in Basal-like TNBC and Circumvents HER3-Associated Resistance via the NEDD4-NDRG1 Axis.

    abstract::Triple-negative breast cancer (TNBC) is a highly aggressive, heterogeneous disease with poor prognosis and no effective targeted therapies. EGFR is highly expressed in basal-like TNBC and is considered as a potential therapeutic target. However, EGFR targeting exerts only marginal clinical benefits, possibly due to ac...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-16-1797

    authors: Verma N,Müller AK,Kothari C,Panayotopoulou E,Kedan A,Selitrennik M,Mills GB,Nguyen LK,Shin S,Karn T,Holtrich U,Lev S

    更新日期:2017-01-01 00:00:00

  • Hypoxanthine concentrations in normal subjects and patients with solid tumors and leukemia.

    abstract::Mean plasma hypoxanthine (Hyp) concentrations determined by high-pressure liquid chromatography were 0.56 microM (range, 0.2 to 1.9 microM) in 16 normal subjects, 0.68 microM (range, 0.1 to 1.1 microM) in 10 untreated acute leukemic subjects, and 0.89 microM (range, 0.3 to 2.6 microM) in 14 solid tumor patients. Despi...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wung WE,Howell SB

    更新日期:1984-07-01 00:00:00

  • Bdp, a new member of a family of DNA-binding proteins, associates with the retinoblastoma gene product.

    abstract::We have cloned a gene, BDP, encoding a protein with homology to the retinoblastoma-binding proteins Rbp1 and Rbp2. It also has homology to DNA-binding proteins such as Bright, a B-cell-specific trans-activator, and the Drosophila melanogaster dead ringer gene product. Like MyoD, Bdp binds to the COOH-terminal region o...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Numata S,Claudio PP,Dean C,Giordano A,Croce CM

    更新日期:1999-08-01 00:00:00

  • Sp3, but not Sp1, mediates the transcriptional activation of the p21/WAF1/Cip1 gene promoter by histone deacetylase inhibitor.

    abstract::We previously reported that both sodium butyrate and trichostatin A (TSA), both of which are known as inhibitors of histone deacetylase, arrest human tumor cells at G1 and G2-M and activate the cyclin-dependent kinase inhibitor, the p21/WAF1/Cip1 gene promoter, through the Sp1 sites. In this study, we identified Sp1 a...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Sowa Y,Orita T,Minamikawa-Hiranabe S,Mizuno T,Nomura H,Sakai T

    更新日期:1999-09-01 00:00:00

  • Monoallelic expression determines oncogenic progression and outcome in benign and malignant brain tumors.

    abstract::Although monoallelic expression (MAE) is a frequent genomic event in normal tissues, its role in tumorigenesis remains unclear. Here we carried out single-nucleotide polymorphism arrays on DNA and RNA from a large cohort of pediatric and adult brain tumor tissues to determine the genome-wide rate of MAE, its role in s...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-2266

    authors: Walker EJ,Zhang C,Castelo-Branco P,Hawkins C,Wilson W,Zhukova N,Alon N,Novokmet A,Baskin B,Ray P,Knobbe C,Dirks P,Taylor MD,Croul S,Malkin D,Tabori U

    更新日期:2012-02-01 00:00:00

  • In vivo radiation protection by nitric oxide modulation.

    abstract::Drugs that affect blood flow have been shown to be whole body radiation protectors. Using NG-nitro-L-arginine, a specific inhibitor of nitric oxide synthase, and the NO-releasing agent (C2H5)2N[N(O)NO-]Na+ (DEA/NO), we have studied the ability of NO to modulate whole body radiation toxicity in C3H mice. NG-Nitro-L-arg...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Liebmann J,DeLuca AM,Coffin D,Keefer LK,Venzon D,Wink DA,Mitchell JB

    更新日期:1994-07-01 00:00:00

  • Transformation of mammary epithelial cells by 3-phosphoinositide- dependent protein kinase-1 activates beta-catenin and c-Myc, and down-regulates caveolin-1.

    abstract::3-phosphoinositide-dependent protein kinase-1 (PDK1) plays a pivotal role in coupling growth factor receptor signaling to tumor cell proliferation, survival, and invasion. Protein kinase C (PKC) alpha, but not Akt1, was found previously to be downstream of PDK1-mediated transformation of mammary epithelial cells. To d...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Xie Z,Zeng X,Waldman T,Glazer RI

    更新日期:2003-09-01 00:00:00

  • Epigenetic transdifferentiation of normal melanocytes by a metastatic melanoma microenvironment.

    abstract::The clinical management of cutaneous melanoma would benefit significantly from a better understanding of the molecular changes that occur during melanocytic progression to a melanoma phenotype. To gain unique insights into this process, we developed a three-dimensional in vitro model that allows observations of normal...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-2497

    authors: Seftor EA,Brown KM,Chin L,Kirschmann DA,Wheaton WW,Protopopov A,Feng B,Balagurunathan Y,Trent JM,Nickoloff BJ,Seftor RE,Hendrix MJ

    更新日期:2005-11-15 00:00:00

  • Adenovirus-based transfer of wild-type p53 gene increases ovarian tumor radiosensitivity.

    abstract::The p53 tumor suppressor gene product is known to be active in mediating radiation-induced G1-S cell cycle arrest and apoptosis in a number of normal cell lines. These functions are compromised by inactivation of p53, which promotes tumor progression. Because the p53 gene appears to play an important role in the cellu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Gallardo D,Drazan KE,McBride WH

    更新日期:1996-11-01 00:00:00

  • Antiangiogenic agent sunitinib transiently increases tumor oxygenation and suppresses cycling hypoxia.

    abstract::Structural and functional abnormalities in tumor blood vessels impact the delivery of oxygen and nutrients to solid tumors, resulting in chronic and cycling hypoxia. Although chronically hypoxic regions exhibit treatment resistance, more recently it has been shown that cycling hypoxic regions acquire prosurvival pathw...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-2025

    authors: Matsumoto S,Batra S,Saito K,Yasui H,Choudhuri R,Gadisetti C,Subramanian S,Devasahayam N,Munasinghe JP,Mitchell JB,Krishna MC

    更新日期:2011-10-15 00:00:00