Intragastric immunization with recombinant Lactobacillus casei expressing flagellar antigen confers antibody-independent protective immunity against Salmonella enterica serovar Enteritidis.

Abstract:

:A recombinant Lactobacillus casei expressing a flagellar antigen from Salmonella enterica serovar Enteritidis was constructed and evaluated as a mucosal vaccine. Intragastric immunization of the recombinant strain conferred protective immunity against Salmonella infection in mice. This immunization did not result in antigen-specific antibody in either feces or sera but induced the release of IFN-gamma on restimulation of primed lymphocytes ex vivo. The results suggested that the protective efficacy provided by flagellin-expressing L. casei is mainly attributable to cell-mediated immune responses. In addition, an adjuvant-type effect of the antigen delivery system with L. casei was also observed.

journal_name

Vaccine

journal_title

Vaccine

authors

Kajikawa A,Satoh E,Leer RJ,Yamamoto S,Igimi S

doi

10.1016/j.vaccine.2007.01.055

subject

Has Abstract

pub_date

2007-05-04 00:00:00

pages

3599-605

issue

18

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(07)00083-7

journal_volume

25

pub_type

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