Abstract:
:We have developed three radioimmunoassays (RIAs) of varying specificities toward the endothelin (ET) isoforms. The assays are called the endothelin-1,2[125I] assay system (RPA535), the endothelin 1-21 Specific [125I] assay system (RPA555), and the endothelin-1,2 high-sensitivity [125I] assay system (RPA545). We have fully characterized their cross-reactivities: RPA535-ED50 approximately 12 fmol/tube, ET-1 100%, ET-2 204%, ET-3 0.0024%, hBig ET 37.9%, pBig ET 32.9%; RPA545-ED50 approximately 4.8 fmol/tube, ET-1 100%, ET-2 1,300%, ET-3 less than 0.001%, hBig ET 189%, pBig ET 63%; RPA555-ED50 approximately 3.6 fmol/tube, ET-1 100%, ET-2 144%, ET-3 52%, hBig ET 0.4%, pBig ET 0.26%. These assays have also been applied to the measurement of ET immunoreactivity (ir) in normal human plasma following Amprep extraction. Further validation of the use of these assays has included the measurement of endothelin-1 (ET-1) derived from big ET by in vitro enzymatic conversion by cathepsin E, which has demonstrated that cathepsin E possesses the appropriate specificity of cleavage to be considered the endothelin-converting enzyme (ECE).
journal_name
J Cardiovasc Pharmacoljournal_title
Journal of cardiovascular pharmacologyauthors
Capper SJ,Ella SJ,Kalinka Sdoi
10.1097/00005344-199100177-00121subject
Has Abstractpub_date
1991-01-01 00:00:00pages
S425-6eissn
0160-2446issn
1533-4023journal_volume
17 Suppl 7pub_type
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