Novel chemical enhancers of heat shock increase thermal radiosensitization through a mitotic catastrophe pathway.

Abstract:

:Radiation therapy combined with adjuvant hyperthermia has the potential to provide outstanding local-regional control for refractory disease. However, achieving therapeutic thermal dose can be problematic. In the current investigation, we used a chemistry-driven approach with the goal of designing and synthesizing novel small molecules that could function as thermal radiosensitizers. (Z)-(+/-)-2-(1-Benzenesulfonylindol-3-ylmethylene)-1-azabicyclo[2.2.2]octan-3-ol was identified as a compound that could lower the threshold for Hsf1 activation and thermal sensitivity. Enhanced thermal sensitivity was associated with significant thermal radiosensitization. We established the structural requirements for activity: the presence of an N-benzenesulfonylindole or N-benzylindole moiety linked at the indolic 3-position to a 2-(1-azabicyclo[2.2.2]octan-3-ol) or 2-(1-azabicyclo[2.2.2]octan-3-one) moiety. These small molecules functioned by exploiting the underlying biophysical events responsible for thermal sensitization. Thermal radiosensitization was characterized biochemically and found to include loss of mitochondrial membrane potential, followed by mitotic catastrophe. These studies identified a novel series of small molecules that represent a promising tool for the treatment of recurrent tumors by ionizing radiation.

journal_name

Cancer Res

journal_title

Cancer research

authors

Sekhar KR,Sonar VN,Muthusamy V,Sasi S,Laszlo A,Sawani J,Horikoshi N,Higashikubo R,Bristow RG,Borrelli MJ,Crooks PA,Lepock JR,Roti Roti JL,Freeman ML

doi

10.1158/0008-5472.CAN-06-3212

subject

Has Abstract

pub_date

2007-01-15 00:00:00

pages

695-701

issue

2

eissn

0008-5472

issn

1538-7445

pii

67/2/695

journal_volume

67

pub_type

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