Abstract:
:The mechanisms responsible for microbially induced epithelial apoptosis and increased intestinal permeability remain unclear. This study assessed whether purified bacterial lipopolysaccharide (LPS) increases epithelial apoptosis and permeability and whether these changes are dependent on caspase-3 activation. In nontumorigenic epithelial monolayers, Escherichia coli O26:B6 LPS increased apoptosis, as shown by nuclear breakdown, caspase-3 activation, and PARP cleavage, and induced disruption of tight junctional ZO-1. Apical, but not basolateral, exposure to LPS increased epithelial permeability. Addition of a caspase-3 inhibitor abolished the effects of LPS. The findings describe a novel mechanism whereby apical LPS may disrupt epithelial tight junctional ZO-1 and barrier function in a caspase-3-dependent fashion.
journal_name
Can J Physiol Pharmacoljournal_title
Canadian journal of physiology and pharmacologyauthors
Chin AC,Flynn AN,Fedwick JP,Buret AGdoi
10.1139/y06-056subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1043-50issue
10eissn
0008-4212issn
1205-7541pii
y06-056journal_volume
84pub_type
杂志文章abstract::Opioid and N-methyl-d-aspartate (NMDA) receptors mediate different effects of fluoxetine. We investigated whether opioid and NMDA receptors are involved in the protective effect of fluoxetine against the behavioral despair induced by acute physical stress in male mice. We used the forced swimming test (FST), tail susp...
journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/cjpp-2015-0429
更新日期:2016-06-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:10.1139/y80-198
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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abstract::The purpose of the present work was to study the rate of basal luteinizing hormone (LH) glycosylation, discriminating the co-translational (proximal) and the post-translational (distal) glycosylation. The experiments were performed to determine the temporal relationship between the biosynthesis of the peptide chains (...
journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
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更新日期:1997-10-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
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doi:10.1139/y95-195
更新日期:1995-09-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
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更新日期:2013-11-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
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doi:10.1139/y02-067
更新日期:2002-06-01 00:00:00
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章,评审
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journal_title:Canadian journal of physiology and pharmacology
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
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journal_title:Canadian journal of physiology and pharmacology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00