Effect of a high fat diet on quantitative features of adipocytes in the omentum: an experimental, stereological and ultrastructural study.

Abstract:

BACKGROUND:Omental adipose tissue specimens of female rats that were fed a high fat (HF) diet were evaluated stereologically and histopathologically. To our knowledge, there is no stereological study on numerical density, nuclear height and volume of adipocytes in omental adipose tissue in the female rat fed a HF diet in the literature. METHOD:20 female Spraque Dawley rats were used in the study. 10 of the animals were fed HF diet consisting of 30% of calories from fat for 3 months. The remaining 10 rats, the control group, were fed a normal diet. After the experimental procedure, all animals were anesthetized and omental adipose tissues in the same area were dissected and fixed for the histochemical process using a mixture of 3% glutaraldehyde and 1% osmium tetraoxide in 0.1 M phosphate buffer. After embedding of tissues in araldite CY 212, semi-thin and thin sections were cut. The semi-thin sections were stained with toluidine blue. The physical dissector counting method was used for estimation of numerical density and nuclear height of adipocytes. Cavalieri principle was used for the estimation of adipocyte volume; volume fraction approach was applied to find the volume fraction of adipose tissue components. RESULTS:The mean numerical density of adipocytes in the HF diet group was significantly higher than the control. The mean nuclear height of adipocytes was also very high in the HF diet group. The volume fraction of adipose mass was increased whereas the extracellular matrix volume fraction was reduced in the HF diet group compared to the controls. The mean volume of adipocytes in the HF diet group was also significantly higher than in the control group. At the light microscopy level, it was found that adipocytes were enlarged and gaining irregular shape in the HF diet group. Thicker basal lamina and electron dense lipid content were also found in this group at the electron microscopy level. CONCLUSION:Lipid content and number of adipocytes in the adipose tissue of HF diet rats were higher than in the controls. Thus, HF diet induces increase in body weight via both hypertrophy and hyperplasia of adipocytes.

journal_name

Obes Surg

journal_title

Obesity surgery

authors

Aslan H,Altunkaynak BZ,Altunkaynak ME,Vuraler O,Kaplan S,Unal B

doi

10.1381/096089206778869942

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

1526-34

issue

11

eissn

0960-8923

issn

1708-0428

journal_volume

16

pub_type

杂志文章
  • Treatment Success: Investigating Clinically Significant Change in Quality of Life Following Bariatric Surgery.

    abstract:BACKGROUND:One of the most important outcomes following bariatric surgery is an improvement in health-related quality of life (QOL). This study aimed to explore what degree of weight loss is required after bariatric surgery in order to achieve a clinically significant change in QOL from pre-surgery to 4 to 5 years afte...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-017-2568-y

    authors: Reynolds CL,Byrne SM,Hamdorf JM

    更新日期:2017-07-01 00:00:00

  • Resolution of Erosive Esophagitis After Conversion from Vertical Sleeve Gastrectomy to Roux-en-Y Gastric Bypass.

    abstract:BACKGROUND:Laparoscopic sleeve gastrectomy (LSG) has become the preferred bariatric procedure in many countries. However, there is one shortcoming of LSG in the long-term follow-up, and this is the onset of gastro-esophageal reflux disease (GERD) and erosive esophagitis (EE). Conversion to Roux-en-Y gastric bypass (RYG...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-020-04913-6

    authors: Lim CH,Lee PC,Lim E,Eng A,Chan WH,Tan HC,Ho E,Kovalik JP,Ganguly S,Tan J

    更新日期:2020-12-01 00:00:00

  • Inversion Technique for the Removal of Partially Covered Self-Expandable Metallic Stents.

    abstract:BACKGROUND AND AIMS:Partially covered self-expandable metallic stents (PCSEMS), although an effective treatment for anastomotic/staple line leaks and strictures, can be difficult to remove. This study examines the effectiveness of the inversion technique for the removal of PCSEMS in the treatment of leaks and stricture...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-017-2811-6

    authors: Hill C,Khalil BK,Barola S,Agnihotri A,Moran RA,Chen YI,Ngamruengphong S,Singh VK,Frame LA,Schweitzer MA,Magnuson TH,Khashab MA,Okolo PI 3rd,Kumbhari V

    更新日期:2018-01-01 00:00:00

  • Beneficial effects of humidified, warmed carbon dioxide insufflation during laparoscopic bariatric surgery: a randomized clinical trial.

    abstract:BACKGROUND:Recent data has shown that the use of warmed, humidified carbon dioxide (CO2) insufflation during laparoscopic surgery may be associated with better outcomes. METHODS:We performed a randomized, doubleblind, prospective controlled clinical trial of 30 patients undergoing laparoscopic Roux-en-Y gastric bypass...

    journal_title:Obesity surgery

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1381/0960892052993530

    authors: Savel RH,Balasubramanya S,Lasheen S,Gaprindashvili T,Arabov E,Fazylov RM,Lazzaro RS,Macura JM

    更新日期:2005-01-01 00:00:00

  • YKL-40 is elevated in morbidly obese patients and declines after weight loss.

    abstract:BACKGROUND:Patients suffering from morbid obesity (MO) have an increased cardiovascular morbidity and mortality. This increased cardiovascular burden is believed to be caused by a sub-inflammatory state through an increased secretion of monocyte chemoattractant protein-1 (MCP-1) by the adipose tissue, resulting in insu...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-009-9917-4

    authors: Hempen M,Kopp HP,Elhenicky M,Höbaus C,Brix JM,Koppensteiner R,Schernthaner G,Schernthaner GH

    更新日期:2009-11-01 00:00:00

  • Obesity and Metabolic Surgery Society of India (OSSI) Recommendations for Bariatric and Metabolic Surgery Practice During the COVID-19 Pandemic.

    abstract::Bariatric and metabolic surgery (BMS), the only effective option for patients with obesity with or without comorbidities, has been stopped temporarily due to the ongoing novel corona virus disease (COVID-19) pandemic. However, there has been a recent change in the governmental strategy of dealing with this virus from ...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-020-04940-3

    authors: Aggarwal S,Mahawar K,Khaitan M,Raj P,Wadhawan R,Dukkipati N,Kular KS,Prasad A,Bhasker AG,Soni V,Madhok B,Baig S,Palaniappan R,Shivaram HV,Goel D,Bindal V,Saggu S,Shrivastava R,Shah S,Dhorepatil S,Khullar R

    更新日期:2020-12-01 00:00:00

  • Self-Reported Eating Disorder Symptoms Before and After Gastric Bypass and Duodenal Switch for Super Obesity--a 5-Year Follow-Up Study.

    abstract:BACKGROUND:This study assessed eating disorder pathology in persons with obesity before and after Roux-en-Y gastric bypass (RYGB) and biliopancreatic diversion with duodenal switch (DS), in a 5-year follow-up study. METHODS:Sixty participants with BMI 50-60 kg/m(2) were randomly assigned to RYGB (n = 31) or DS (n = 29...

    journal_title:Obesity surgery

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s11695-015-1790-8

    authors: Morseth MS,Hanvold SE,Rø Ø,Risstad H,Mala T,Benth JŠ,Engström M,Olbers T,Henjum S

    更新日期:2016-03-01 00:00:00

  • Exercise testing in individuals with morbid obesity.

    abstract:BACKGROUND:Obesity is an independent risk factor for cardiovascular disease, especially in its morbid form. Bariatric surgery is an effective therapeutic option for sustained weight loss. However, there is no consensus in these cases regarding the performance of functional tests for preoperative evaluations. The aim of...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-008-9692-7

    authors: Fornitano LD,Godoy MF

    更新日期:2010-05-01 00:00:00

  • Technique of Hill's Gastropexy Combined with Sleeve Gastrectomy for Patients with Morbid Obesity and Gastroesophageal Reflux Disease or Hiatal Hernia.

    abstract:BACKGROUND:Observational studies based on quality-of-life and endoscopy relate sleeve gastrectomy (SG) to gastroesophageal reflux disease (GERD), while some functional studies have demonstrated a decrease in esophageal exposure to gastric acid after SG. Currently, it is recommended to treat hiatal hernia along with the...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-016-2076-5

    authors: Sánchez-Pernaute A,Talavera P,Pérez-Aguirre E,Domínguez-Serrano I,Rubio MÁ,Torres A

    更新日期:2016-04-01 00:00:00

  • The Impact of Bariatric Surgery on Cardiopulmonary Function: Analyzing VO2 Recovery Kinetics.

    abstract:PURPOSE:To assess cardiopulmonary capacity, autonomic heart function, and oxygen recovery kinetics during exercise testing before and after bariatric surgery. METHODS:This is a prospective cohort study. Symptom-limited cardiopulmonary exercise testing was performed with 24 patients, 1 week before and 4 months after ba...

    journal_title:Obesity surgery

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11695-018-3469-4

    authors: Remígio MI,Santa Cruz F,Ferraz Á,Remígio MC,Parente G,Nascimento I,Brandão D,Dornelas de Andrade AF,de Moraes Neto F,Campos J

    更新日期:2018-12-01 00:00:00

  • TGR5 Protects Against Colitis in Mice, but Vertical Sleeve Gastrectomy Increases Colitis Severity.

    abstract:BACKGROUND AND AIMS:Bariatric surgery, such as vertical sleeve gastrectomy (VSG), is the most effective long-term treatment for obesity. However, there are conflicting reports on the effect of bariatric surgery on inflammatory bowel disease (IBD). Bariatric surgery increases bile acid concentrations, which can decrease...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-019-03707-9

    authors: Garibay D,Zaborska KE,Shanahan M,Zheng Q,Kelly KM,Montrose DC,Dannenberg AJ,Miller AD,Sethupathy P,Cummings BP

    更新日期:2019-05-01 00:00:00

  • Impact of Bariatric Surgery on Heme Oxygenase-1, Inflammation, and Insulin Resistance in Morbid Obesity with Obstructive Sleep Apnea.

    abstract:INTRODUCTION:Morbid obesity and obstructive sleep apnea (OSA) interact at an inflammatory level. Bariatric surgery reduces inflammatory responses associated with obesity. Heme oxygenase-1 (HO-1) is an enzyme with anti-inflammatory properties, which might be increased in morbid obesity or OSA. We studied morbidly obese ...

    journal_title:Obesity surgery

    pub_type: 临床试验,杂志文章

    doi:10.1007/s11695-017-2635-4

    authors: Tirado R,Masdeu MJ,Vigil L,Rigla M,Luna A,Rebasa P,Pareja R,Hurtado M,Caixàs A

    更新日期:2017-09-01 00:00:00

  • Pre- and postoperative esophageal and gastric pH levels in morbidly obese patients undergoing vertical gastroplasty.

    abstract:BACKGROUND:The aim of this study was to determine whether the anatomicofunctional changes induced by vertical gastroplasty lead to pH alterations in the esophagus and body and antrum of the stomach. MATERIALS AND METHODS:23 non-smoking volunteers (3 men, 20 women) mean age 35.29 +/- SD 6.85 years, mean weight 163.17 +...

    journal_title:Obesity surgery

    pub_type: 临床试验,杂志文章

    doi:10.1381/096089204322857690

    authors: Papavramidis TS,Papavramidis ST,Sapalidis KG,Kessisoglou II,Gamvros OI

    更新日期:2004-02-01 00:00:00

  • Laparoscopic placement of non-adjustable silicone ring for weight regain after Roux-en-Y gastric bypass.

    abstract:BACKGROUND:Roux-en-Y gastric bypass (RYGBP) is presently one of the most popular surgical procedures for obesity. One of the possible long-term problems is weight regain, usually after a period of successful weight loss. Weight regain after RYGBP can be due to new eating habits, like sweet-eating or grazing, or volume ...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-009-9807-9

    authors: Dapri G,Cadière GB,Himpens J

    更新日期:2009-05-01 00:00:00

  • Does Metabolic Surgery Lead to Diabetes Remission in Patients with BMI

    abstract:BACKGROUND:Bariatric surgery has demonstrated to be effective in remission of type 2 diabetes in obese patients, but it is unclear in non-obese patients. The aim of this study is to investigate if metabolic surgery is effective in diabetes resolution in patients with BMI < 30 kg/m2. MATERIALS AND METHODS:A systematic ...

    journal_title:Obesity surgery

    pub_type: 杂志文章,meta分析

    doi:10.1007/s11695-018-03654-x

    authors: Rubio-Almanza M,Hervás-Marín D,Cámara-Gómez R,Caudet-Esteban J,Merino-Torres JF

    更新日期:2019-04-01 00:00:00

  • Impact on obesity-related illnesses and quality of life following intragastric balloon.

    abstract:BACKGROUND:The impact of intragastric balloon (IGB) on obesity-related illnesses and quality of life (QOL) has not been previously investigated. METHOD:One hundred and nineteen consecutive obese patients (86 females; mean age 37.8; mean body weight (BW) 103.7 +/- 24.1 kg; mean BMI 38.4 +/- 8.0 kg/m(2)) who underwent I...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-008-9766-6

    authors: Mui WL,Ng EK,Tsung BY,Lam CH,Yung MY

    更新日期:2010-08-01 00:00:00

  • Is Routine Invasive Monitoring Indicated in Surgery for the Morbidly Obese?

    abstract::BACKGROUND: Morbid obesity is generally considered to be a surgical and anesthetic risk. Some surgeons have advised the routine use of invasive monitoring for morbidly obese individuals undergoing surgery. The purpose of this study was to identify morbidly obese individuals undergoing primary gastric bypass procedures...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1381/096089296765557268

    authors: Capella JF,Capella RF

    更新日期:1996-02-01 00:00:00

  • Glycemic Control after Sleeve Gastrectomy and Roux-En-Y Gastric Bypass in Obese Subjects with Type 2 Diabetes Mellitus.

    abstract:BACKGROUND:Roux-en-Y gastric bypass (LRYGB) has weight-independent effects on glycemia in obese type 2 diabetic patients, whereas sleeve gastrectomy (LSG) is less well characterized. This study aims to compare early weight-independent and later weight-dependent glycemic effects of LRYGB and LSG. METHODS:Eighteen LRYGB...

    journal_title:Obesity surgery

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1007/s11695-017-3061-3

    authors: Wallenius V,Dirinck E,Fändriks L,Maleckas A,le Roux CW,Thorell A

    更新日期:2018-06-01 00:00:00

  • Impact of Oral-Cecal Transit Time on the Interpretation of Lactulose Breath Tests After RYGB: a Personalized Approach to the Diagnosis of SIBO.

    abstract:BACKGROUND:Traditionally, small intestinal bacterial overgrowth (SIBO) is diagnosed when there is an early peak in breath hydrogen or methane. Given unclear intestinal transit time in Roux-en-Y gastric bypass (RYGB) patients, it is unknown if the traditional approach at diagnosing SIBO is adequate in this patient popul...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-018-3575-3

    authors: Jirapinyo P,Makuvire TT,Dong WY,Chan WW,Thompson CC

    更新日期:2019-03-01 00:00:00

  • Biliopancreatic Diversion with a New Type of Gastrectomy.

    abstract::In an attempt to improve the results of biliopancreatic diversion in the treatment of morbid obesity, two aspects of the procedure performed at Laval Hospital were modified to reduce adverse physiological consequences. The distal gastrectomy was replaced by a parietal gastrectomy which preserves vagal continuity along...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1381/096089293765559728

    authors: Marceau P,Biron S,Bourque RA,Potvin M,Hould FS,Simard S

    更新日期:1993-02-01 00:00:00

  • No Difference in Ghrelin-Producing Cell Expression in Obese Versus Non-obese Stomach: a Prospective Histopathological Case-Control Study.

    abstract:BACKGROUND:To understand the role of ghrelin in the mechanism of action of laparoscopic sleeve gastrectomy (LSG), a prospective cohort case-control study to assess the expression of ghrelin-producing cells (GPC) in two groups of patients was designed. METHODS:Specimens of resected stomach from 26 obese patients who un...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-018-3401-y

    authors: Musella M,Di Capua F,D'Armiento M,Velotti N,Bocchetti A,Di Lauro K,Galloro G,Campione S,Petrella G,D'Armiento FP

    更新日期:2018-11-01 00:00:00

  • Upper Gastrointestinal Endoscopy prior to Bariatric Surgery-Mandatory or Expendable? An Analysis of 801 Cases.

    abstract:BACKGROUND:Upper gastrointestinal pathologies are common in bariatric patients. Preoperative esophagogastroduodenal endoscopy (EGD) should detect and treat pathologies that might alter the type of bariatric surgery. However, clinical consequences of these findings are often insignificant. The aim of this study was to a...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-017-2622-9

    authors: Wolter S,Duprée A,Miro J,Schroeder C,Jansen MI,Schulze-Zur-Wiesch C,Groth S,Izbicki J,Mann O,Busch P

    更新日期:2017-08-01 00:00:00

  • The Impact of Robotics in Learning Roux-en-Y Gastric Bypass: a Retrospective Analysis of 214 Laparoscopic and Robotic Procedures : Robotic Vs. Laparoscopic RYGB.

    abstract:BACKGROUND:Proximal Roux-en-Y gastric bypass is commonly used to manage obesity, performed using laparoscopic or robot-assisted minimally invasive surgery. As the prevalence of robotic bariatric surgery increases, further data is required to justify its use. METHODS:This was a large, retrospective analysis of prospect...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-020-04508-1

    authors: Beckmann JH,Bernsmeier A,Kersebaum JN,Mehdorn AS,von Schönfels W,Taivankhuu T,Laudes M,Schafmayer C,Egberts JH,Becker T

    更新日期:2020-06-01 00:00:00

  • Bleeding from the upper gastrointestinal tract after Mason's vertical banded gastroplasty.

    abstract:BACKGROUND:Gastric bleeding is a rare complication after a vertical banded gastroplasty (VBG). Only a few cases of gastric bleeding after a VBG have been reported, and there is discussion about its etiology. We present two cases of gastric bleeding after a VBG, and discuss the etiology, diagnostic approach and manageme...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1381/096089200321594219

    authors: Papakonstantinou A,Terzis L,Stratopoulos C,Mablekou E,Papadimitriou N,Hadjiyannakis E

    更新日期:2000-12-01 00:00:00

  • Pouch dilatation after gastric banding causing gastric necrosis.

    abstract:BACKGROUND:Early or late pouch dilatations account for a moderate complication rate after restrictive bariatric operations. Various strategies were developed to prevent or treat pouch dilatations. METHODS:A case of necrosis of gastric wall in a concentric dilated pouch following laparoscopic adjustable gastric banding...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1381/09608920160558768

    authors: Kirchmayr W,Ammann K,Aigner F,Weiss HG,Nehoda H

    更新日期:2001-12-01 00:00:00

  • Laparoscopic reoperative sleeve gastrectomy for poor weight loss after biliopancreatic diversion with duodenal switch.

    abstract:BACKGROUND:The revisional surgery for patients with inadequate weight loss after biliopancreatic diversion with duodenal switch (BPD/DS) is controversial. It has not yet been determined whether a common channel should be shortened or gastric pouch volume reduced. Since the revision of the distal anastomosis remains tec...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1381/096089203322190907

    authors: Gagner M,Rogula T

    更新日期:2003-08-01 00:00:00

  • Preoperative and Postoperative Assessments of Biochemical Parameters in Patients with Severe Obesity Undergoing Laparoscopic Sleeve Gastrectomy.

    abstract:BACKGROUND:The study aim was to determine the prevalence of abnormal serum biochemistries associated with micronutrient deficiencies before and after laparoscopic sleeve gastrectomy (LSG). METHODS:Two hundred and one patients had LSG surgery between May 2011 and May 2014. Using a prospective cohort study design, data ...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-017-3007-9

    authors: Gregory DM,Twells LK,Lester KK,Midodzi WK,Pedersen MR,Pace D,Smith C,Boone D,Randell EW,Kovacs CS

    更新日期:2018-08-01 00:00:00

  • Outcomes, satiety, and adverse upper gastrointestinal symptoms following laparoscopic adjustable gastric banding.

    abstract:BACKGROUND:Follow-up is critical to the success of laparoscopic adjustable gastric banding (LAGB). Few data guide this and expected norms of satiety, adverse symptoms, and outcomes have not been defined. METHODS:Consecutive patients, who underwent LAGB, were evaluated using a newly developed instrument that assessed s...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-010-0073-7

    authors: Burton PR,Brown W,Laurie C,Lee M,Korin A,Anderson M,Hebbard G,O'Brien PE

    更新日期:2011-05-01 00:00:00

  • Morbidity in patients with or at high risk for obstructive sleep apnea after ambulatory laparoscopic gastric banding.

    abstract::Considerable controversy exists about the perioperative management of patients at high risk for obstructive sleep apnea (OSA) in free-standing clinics. Eighty-eight percent of an American Society of Anesthesiologists expert panel felt that upper abdominal laparoscopic surgery could not be performed safely on an outpat...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1007/s11695-011-0381-6

    authors: Kurrek MM,Cobourn C,Wojtasik Z,Kiss A,Dain SL

    更新日期:2011-10-01 00:00:00

  • Some complications after laparoscopic nonadjustable gastric banding.

    abstract:BACKGROUND:Bariatric surgery is the only currently available, effective, long-term method for controlling morbid obesity. Gastric banding as one of the possible surgical treatments was repeatedly described during the last 10 years. It is a reversible surgical procedure which is primarily performed laparoscopically. ME...

    journal_title:Obesity surgery

    pub_type: 杂志文章

    doi:10.1381/096089299765552710

    authors: Kasalicky M,Fried M,Peskova M

    更新日期:1999-10-01 00:00:00