Abstract:
:The aim of the present study was to investigate the influence of 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model of primary culture of porcine aortic endothelial cells (PAEC). We took into consideration the Hsp profile in terms of mRNA expression, protein expression and protein localization inside the cells. The choice of the cell system was motivated by the involvement of the endothelial cells in the onset of many diseases; moreover, only few reports describe the effects of extremely low frequency magnetic fields (ELF-MFs) on such cells. ELF-MF exposure induced an increase in the mRNA levels of the three proteins, which was statistically significant for Hsp70. On the contrary, we did not observe any influence on Hsp27, Hsp70, and Hsp90 protein levels. Analysis in situ by immunofluorescence revealed that ELF-MF exposure affected the cellular distribution of Hsp27; in particular a partial relocalization in the nucleus was observed.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Bernardini C,Zannoni A,Turba ME,Bacci ML,Forni M,Mesirca P,Remondini D,Castellani G,Bersani Fdoi
10.1002/bem.20299subject
Has Abstractpub_date
2007-04-01 00:00:00pages
231-7issue
3eissn
0197-8462issn
1521-186Xjournal_volume
28pub_type
杂志文章abstract::Several effects of power-frequency (50/60 Hz) magnetic fields (PF-MF) of weak intensity have been hypothesized in animals and humans. No valid mechanism, however, has been proposed for an interaction between PF-MF and biological tissues and living beings at intensities relevant to animal and human exposure. Here we pr...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20568
更新日期:2010-07-01 00:00:00
abstract::There are large disparities between basic restrictions for exposure to extremely low-frequency (0-3 kHz) Electric and Magnetic Fields set by two major international bodies. Both bodies agree that these basic restrictions should prevent neuro-stimulatory effects: the retinal phosphene at frequencies up to a few hundred...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20412
更新日期:2008-09-01 00:00:00
abstract::We have exposed cultured bone cells to a pulsed electromagnetic field (PEMF) for different times to find the minimal exposure time necessary to stimulate an increase of DNA synthesis. We used two different human osteosarcoma cell lines, TE-85 and MG-63, and human normal osteoblast cell (NHOC) obtained from surgical bo...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1999)20:3<177::aid-bem4>3.
更新日期:1999-01-01 00:00:00
abstract::The constant exposure of modern society to extremely low frequency magnetic fields (ELF-MF) has raised considerable concerns about the potential risks to male reproduction. However, the epidemiological and experimental data remain contradictory and inconclusive. In the present study, we investigated the effects of 50 ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21816
更新日期:2014-01-01 00:00:00
abstract::We developed a new computer wire coding method and then applied it to investigate the suggestion that control selection bias might explain the observed association between wire codes and childhood cancer made in the study conducted by Savitz et al. in the Denver area. The computer wire coding method used a geographic ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::This study examines childhood cancer risk in relation to certain factors likely to indicate magnetic field exposure from ground currents in the home. Substantial ground currents are most often found in homes having conductive plumbing, in which an uninterrupted metallic path in the water pipes and water main connects ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160204
更新日期:1995-01-01 00:00:00
abstract::We sought to determine whether a 6-week exposure to a 50-Hz rotating magnetic field influences melatonin synthesis by 11-18 week-old Wistar-King male rats. Rats were exposed continuously to a rotating magnetic field at 1, 5, 50, or 250 microT (spatial vector rms) for 6 weeks, except for twice-weekly breaks of about 2 ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250140203
更新日期:1993-01-01 00:00:00
abstract::The impedance spectroscopy technique (IST) was used for studying the effect of a 0.5 T magnetic field on the electrical properties of whole human blood. A Solartron SI 1260 spectrometer was used to measure the impedance spectra of magnetic field exposed blood samples compared to non-exposed samples. An equivalent elec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20132
更新日期:2005-10-01 00:00:00
abstract::The controversy over the possible association between magnetic field exposure and childhood leukemia has led several researchers to summarize the literature using meta-analysis. This paper reviews these previous meta-analyses and extends them by adding results from four studies published since the most recent analysis...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,meta分析
doi:10.1002/1521-186x(2001)22:5+<::aid-bem1026>3.3.co;
更新日期:2001-01-01 00:00:00
abstract::Serial longitudinal and transverse sections were prepared from roots of Cucumis sativus and Cucurbita maxima that had been exposed/sham-exposed to 60-Hz electric fields for 0-2 days. Field exposures were selected to produce a 10-20% or a 70-80% growth inhibition in whole roots of both species. Cortical cell length and...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250080108
更新日期:1987-01-01 00:00:00
abstract::The dielectric behavior of the aqueous solutions of three widely differing macromolecules has been investigated: myoglobin, polyvinylpyrrolidone (PVP), and human serum low-density lipoprotein (LDL). It was not possible to interpret unambiguously the dielectric properties of the PVP solution in terms of water structure...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070206
更新日期:1986-01-01 00:00:00
abstract::The effects of hyperthermia, alone and in conjunction with microwave exposure, on brain energetics were studied in anesthetized male Sprague-Dawley rats. The effect of temperature on adenosine triphosphate concentration [ATP] and creatine phosphate concentration [CP] was determined in the brains of rats that were main...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050107
更新日期:1984-01-01 00:00:00
abstract::Recent interest in the transient magnetic field events produced by electrical switching events in residential and occupational environments has been kindled by the possibility that these fields may explain observed associations between childhood cancer and wire codes. This paper reports the results of a study in which...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(200004)21:3<197::aid-bem7>
更新日期:2000-04-01 00:00:00
abstract::The wide and growing use of cellular phones has raised questions about the possible health risks associated with radio frequency (RF) electromagnetic fields. It would be helpful for epidemiologists as well as cellular phone users to obtain the relative exposure levels, because the RF exposure level is very difficult t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20203
更新日期:2006-09-01 00:00:00
abstract::Calcium (45Ca2+) efflux from preloaded synaptosomes was studied with a continuous perfusion technique and the rate constants of a two-phase efflux process calculated. When 16-Hz sinusoidally amplitude modulated 450-MHz microwave field (maximal incident intensity 0.5 mW/cm2, modulation depth 75%) was applied during the...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250030303
更新日期:1982-01-01 00:00:00
abstract::Using a crossover experimental design, we evaluated our earlier findings that exposure to a 30 kV/m, 60 Hz electric field for 12 hours per day, 7 days per week for 6 weeks produced significant changes in the performance rates of social behaviors among young adult male baboons. In the crossover experiment, the former c...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130506
更新日期:1992-01-01 00:00:00
abstract::We summarize the results of several of our recent studies on the dielectric properties of protein solutions, tissues, and nonionic microemulsions at microwave frequencies extending to 18 GHz. The data in all cases are analyzed using the Maxwell mixture theory to determine the dielectric properties of the suspending wa...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250030108
更新日期:1982-01-01 00:00:00
abstract::Recent work has demonstrated that the reflection coefficient of human skin in the frequency range from 95 to 110 GHz (W band) mirrors the temporal relaxation of stress induced by physical exercise. In this work, we extend these findings to show that in the event of a subtle trigger to stress, such as mental activity, ...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章
doi:10.1002/bem.21698
更新日期:2012-07-01 00:00:00
abstract::Findings from prior studies of possible health and physiological effects from mobile phone use have been inconsistent. Exposure periods in provocation studies have been rather short and personal characteristics of the participants poorly defined. We studied the effect of radiofrequency field (RF) on self-reported symp...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20379
更新日期:2008-04-01 00:00:00
abstract::Low-energy pulsed electromagnetic field (PEMF) therapy has been suggested as a promising therapy to increase microcirculation, which is of great concern in patients with fibromyalgia. This study evaluated the effectiveness of PEMF therapy on the treatment of fibromyalgia. A group of 108 women with fibromyalgia were al...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,随机对照试验
doi:10.1002/bem.22127
更新日期:2018-07-01 00:00:00
abstract::The mechanism by which animals detect weak electric and magnetic fields has not yet been elucidated. We propose that transduction of an electric field (E) occurs at the apical membrane of a specialized cell as a consequence of an interaction between the field and glycoproteins bound to the gates of ion channels. Accor...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20310
更新日期:2007-07-01 00:00:00
abstract::Mobile phone use in the United Kingdom and other countries has risen steeply since the early 1990's when the first digital mobile phones were introduced. There is an ongoing controversy about whether radio frequency (RF) exposure from mobile phones increases the risk of brain cancer. However, given the widespread use ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20648
更新日期:2011-07-01 00:00:00
abstract::One hundred subjects, males and females with ages ranging between 18 and 48 years, were studied under both field-exposed and sham-exposed conditions. A 50 Hz, 100 mu T magnetic field (MF) was used. To examine the effect of field exposure on performance, a two-alternative, forced-choice, duration-discrimination task wi...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/(SICI)1521-186X(1996)17:2<131::AID-BEM7>3.
更新日期:1996-01-01 00:00:00
abstract::We have previously demonstrated that exposure of Chinese hamster lung (CHL) cells to 50 Hz magnetic fields (MFs) and/or 12-O-tetradecanoylphorbol-3-acetate (TPA)-inhibited gap junctional intercellular communication (GJIC). To explore and compare the mechanisms of GJIC inhibition induced by extremely low frequency (ELF...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10070
更新日期:2003-02-01 00:00:00
abstract::An elementary model consisting of one charged particle in a viscous medium exposed to weak ac-dc low-frequency magnetic fields is analyzed to identify and explain the fundamental characteristics of the physical mechanisms that result in a resonance response, which is similar to the familiar cyclotron resonance. The mo...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250090402
更新日期:1988-01-01 00:00:00
abstract::A number of studies have shown that power frequency magnetic fields may affect spatial memory functions in rodents. An experiment was performed using a spontaneous object recognition task to investigate if nonspatial working memory was similarly affected. Memory changes in adult, male C57BL/6J mice were assessed by me...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/1521-186x(200101)22:1<19::aid-bem3>3.0.co;
更新日期:2001-01-01 00:00:00
abstract::The temporal variation of a static electric field inside an animal cage was investigated with a newly developed small, simple field meter. The field inside the cage was found to be highly dependent on the surface conductivity of the dielectric material. As the surface of the cage became dirty because of animal occupan...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250020410
更新日期:1981-01-01 00:00:00
abstract::This study deals with the morphofunctional influence of 72 h exposure to a 6 mT static magnetic field (SMF) during differentiation induced by 50 ng/ml 12-O-tetradecanoyl-13-phorbol acetate (TPA) in human leukaemia U937 cells. The cell morphology of U937 cells was investigated by optic and electron microscopy. Specific...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20474
更新日期:2009-07-01 00:00:00
abstract::Published and new data for grounded humans, swine, and rats exposed to vertical, 60-Hz electric fields are used to determine field strengths at the surfaces of the bodies and average components of induced-current density along the axes of the bodies. At the tops of the bodies, surface electric fields are increased (en...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250010203
更新日期:1980-01-01 00:00:00
abstract::Studies have evaluated the electroencephalography (EEG) of humans and laboratory animals during and after Radiofrequency (RF) exposures. Effects of RF exposure on the blood-brain barrier (BBB) have been generally accepted for exposures that are thermalizing. Low level exposures that report alterations of the BBB remai...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/bem.10179
更新日期:2003-01-01 00:00:00