Effects of 50 Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC).

Abstract:

:The aim of the present study was to investigate the influence of 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model of primary culture of porcine aortic endothelial cells (PAEC). We took into consideration the Hsp profile in terms of mRNA expression, protein expression and protein localization inside the cells. The choice of the cell system was motivated by the involvement of the endothelial cells in the onset of many diseases; moreover, only few reports describe the effects of extremely low frequency magnetic fields (ELF-MFs) on such cells. ELF-MF exposure induced an increase in the mRNA levels of the three proteins, which was statistically significant for Hsp70. On the contrary, we did not observe any influence on Hsp27, Hsp70, and Hsp90 protein levels. Analysis in situ by immunofluorescence revealed that ELF-MF exposure affected the cellular distribution of Hsp27; in particular a partial relocalization in the nucleus was observed.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

Bernardini C,Zannoni A,Turba ME,Bacci ML,Forni M,Mesirca P,Remondini D,Castellani G,Bersani F

doi

10.1002/bem.20299

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

231-7

issue

3

eissn

0197-8462

issn

1521-186X

journal_volume

28

pub_type

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