Abstract:
:In previous studies examining the role of pp60c-src in cellular proliferation, we demonstrated that overexpression of wild type (wt) but not mutated c-src in C3H10T1/2 murine embryo fibroblasts resulted in a 2 to 5 fold enhancement of DNA synthesis in response to epidermal growth factor (EGF). Using phosphotyrosine antiserum to probe Western immunoblots of whole cell lysates, we determined in the present study that proteins migrating at approximately 170 (EGF receptor), 125, 100, 75, 63 and 57 kDa exhibited EGF-dependent tyrosine phosphorylations that were approximately 3 fold higher in the wt c-src overexpressors than in control cells, while proteins of 125, 100 and 75 kDa displayed basal, unstimulated levels that were approximately 2 fold higher. Pp60c-src-associated hyperphosphorylation of all but the 63 and 57 kDa proteins was reduced or ablated in cells overproducing structurally-altered forms of c-src. Furthermore, in contrast to control cells, wt c-src overexpressors retained EGF-induced tyrosine phosphorylations on the 125, 100 and 75 kDa proteins for up to 9 h of EGF treatment. These results illuminate potential substrates for pp60c-src and suggest that overproduced pp60c-src potentiates the EGF mitogenic response by increasing the extent and duration of tyrosine phosphorylations on proteins involved in EGF mitogenic signalling.
journal_name
Oncogenejournal_title
Oncogeneauthors
Wilson LK,Parsons SJsubject
Has Abstractpub_date
1990-10-01 00:00:00pages
1471-80issue
10eissn
0950-9232issn
1476-5594journal_volume
5pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::The integrin linked kinase (ILK) is a cytoplasmic effector of integrin receptors, involved in the regulation of integrin binding properties as well as the activation of cell survival and proliferative pathways, including those involving MAP kinase, PKB/Akt and GSK-3beta. Overexpression of ILK in cultured intestinal an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204910
更新日期:2001-10-25 00:00:00
abstract::Although apoptosis can be induced by the enforced expression of exogenously introduced c-myc genes, it is not clear whether overexpression resulting from the amplification of the resident c-myc gene in tumor cells is sufficient to induce apoptosis. We have investigated the relationship between c-myc gene amplification...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202309
更新日期:1999-01-14 00:00:00
abstract::We have previously demonstrated the existence of a melanoma tumor suppressor gene(s) on the long arm of chromosome 11 through suppression of tumorigenicity assays. Although loss of heterozygosity studies also support this finding, only a large critical region (44 cM) has been identified to date on 11q22-25. To further...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202664
更新日期:1999-05-20 00:00:00
abstract::The putative tumor suppressor CDKN1C is an imprinted gene at 11p15.5, a well-known imprinted region often deleted in tumors. The absence of somatic mutations and the frequent diminished expression in tumors would suggest that CDKN1C expression is regulated epigenetically. It has been, however, controversial whether th...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207576
更新日期:2004-05-27 00:00:00
abstract::As part of a cloning strategy to identify genes involved in early mouse liver development we have isolated Praja1, a gene with similar sequences to the Drosophila melanogaster gene goliath (gl) which is involved in the fate of mesodermal cells ultimately forming gut musculatures, fat body, and the heart. Praja1 is a 2...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201405
更新日期:1997-11-06 00:00:00
abstract::A proportion of familial breast cancer has recently been shown by genetic linkage analysis to map to chromosome l3q12 (Wooster et al, 1994). This locus contains a tumor suppressor gene BRCA2, mutations in which lead to tumorigenesis. Genetic alterations at this locus have also been shown in pancreatic adenocarcinoma a...
journal_title:Oncogene
pub_type:
doi:
更新日期:1996-07-04 00:00:00
abstract::The acute contractile function of the heart is controlled by the effects of released nonepinephrine (NE) on cardiac adrenergic receptors. NE can also act in a more chronic fashion to induce cardiomyocyte growth, characterized by cell enlargement (hypertrophy), increased protein synthesis, alterations in gene expressio...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1204275
更新日期:2001-03-26 00:00:00
abstract::Most human lymphomas originate from transformed germinal center (GC) B lymphocytes. While activating mutations and translocations of MYC, BCL2 and BCL6 promote specific GC lymphoma subtypes, other genetic and epigenetic modifications that contribute to malignant progression in the GC remain poorly defined. Recently, a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210211
更新日期:2007-06-21 00:00:00
abstract::The p53 tumor suppressor protein plays a critical role in mediating cellular response to stress. Upon DNA damage, post-translational modifications stabilize and activate this nuclear phosphoprotein. To determine the effect of phosphorylation site mutants in the context of the whole p53 protein, we performed reporter a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207005
更新日期:2004-01-08 00:00:00
abstract::Pdcd4 is a novel transformation suppressor that is highly expressed in promotion-resistant (P-) mouse epidermal JB6 cells but not in susceptible (P+) cells. Overexpression of pdcd4 cDNA in stably transfected P+ cells rendered cells resistant to tumor promoter-induced transformation, indicating that elevated expression...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204137
更新日期:2001-02-08 00:00:00
abstract::The role of p53 in genotoxic therapy-induced metabolic shift in cancers is not yet known. In this study, we investigated the role of p53 in the glycolytic shift in head and neck squamous cell carcinoma cell lines following irradiation. Isogenic p53-null radioresistant cancer cells established through cumulative irradi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0697-6
更新日期:2019-05-01 00:00:00
abstract::The discovery of constitutive nuclear factor-κB (NF-κB) activation in Hodgkin's lymphoma tumor cells almost two decades ago was one of the first reports that directly connected deregulated NF-κB signaling to human cancer. Subsequent studies demonstrated that enhanced NF-κB signaling is a common hallmark of many lympho...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2013.565
更新日期:2014-12-11 00:00:00
abstract::The Neuregulins and their receptors, the ErbB/HER subfamily of receptor tyrosine kinases, have critical roles in animal physiology, and their deregulation is frequent in cancer. Here we report the identification of the guanine nucleotide exchange factor, phosphatidylinositol 3,4,5-triphosphate-dependent Rac exchanger ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.489
更新日期:2011-03-03 00:00:00
abstract::Signal transducers and activators of transcription (Stats) are known to transduce signals from the cell surface to the nucleus in cytokine receptor signaling. We examined the capacity of platelet-derived growth factor (PDGF) receptor to interact with and activate Stat molecules. Activation of the PDGF beta-receptor le...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201555
更新日期:1998-01-29 00:00:00
abstract::Suppression of detachment-induced cell death, known as anoikis, is an essential step for cancer metastasis to occur. We report here that expression of KLF12, a member of the Kruppel-like family of transcription factors, is downregulated in lung cancer cell lines that have been selected to grow in the absence of cell a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.394
更新日期:2016-06-23 00:00:00
abstract::Tumors consistently mimic wound-generating chronic inflammation; however, why they do not heal like wounds with fibrotic scars remains unknown. The components of the tumor microenvironment, such as transforming growth factor β (TGF-β) and fibroblast growth factors (FGFs), may account for this phenomenon. Tumor formati...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2016.512
更新日期:2017-07-06 00:00:00
abstract::Better understanding of the underlying biology of malignant gliomas is critical for the development of early detection strategies and new therapeutics. This study aimed to define genes associated with survival. We investigated whether genes coupled with a class prediction model could be used to define subgroups of hig...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209585
更新日期:2006-09-28 00:00:00
abstract::Polyomavirus infection of adolescent athymic female mice causes a high incidence of mammary adenocarcinomas. We have examined the role of ovarian hormones, age and mammary gland developmental stage at infection on subsequent tumor induction, viral replication and gene expression. Ovariectomy (OVX) of adolescent mice 1...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-11-02 00:00:00
abstract::The v-erbA oncogene transforms chicken erythrocytic progenitors (T2EC) by blocking their differentiation and freezing them in a state of self-renewal. Transcriptomes of T2EC, expressing either v-erbA or a non-transforming form of v-erbA (S61G), were compared using serial analysis of gene expression and some, but not a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.93
更新日期:2014-03-20 00:00:00
abstract::MicroRNAs (miRNAs) have been shown to have an important role in various cellular processes, such as apoptosis, differentiation and development. Recent studies have shown that miRNAs are mis-expressed in human cancers where they can exert their effect as oncogenes or tumor suppressors. Here, we review the potential for...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2009.353
更新日期:2008-12-01 00:00:00
abstract::Matrix metalloproteinase-2 (MMP-2) has pivotal role in the degradation of extracellular matrix, and thereby enhances the invasive, proliferative and metastatic potential in cancer. Knockdown of MMP-2 using MMP-2 small interfering RNA (pM) in human glioma xenograft cell lines 4910 and 5310 decreased cell proliferation ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.52
更新日期:2013-01-17 00:00:00
abstract::Overexpression of HER-2/neu (also known as c-erbB-2) proto-oncogene frequently occurs in many different types of human cancers, including ovarian carcinoma, and is known to enhance tumor metastasis and chemoresistance. Previous studies showed that inhibition of HER-2/neu expression by various agents, such as adenoviru...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203582
更新日期:2000-05-18 00:00:00
abstract::Tumor suppressor p53 functions are downregulated in most cervical cancers, because the product of human papilloma virus (HPV) oncogene E6 binds to and inactivates p53 by promoting its degradation. p73, a p53 homologue, is similar to p53 in structure and function but yet not degraded by HPV E6 gene product. In this stu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206908
更新日期:2003-11-20 00:00:00
abstract::Stimulation of the c-Kit receptor tyrosine kinase has a critical role in the development and migration of melanocytes, and oncogenic c-Kit mutants contribute to the progression of some melanomas. c-Kit signalling activates the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways an...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.562
更新日期:2014-01-09 00:00:00
abstract::Activating the protein-tyrosine kinase activity of v-Src rapidly induced expression of the two 'primary response' genes, TIS10 and Egr-1, in Balb/c 3T3 cells. Depleting cells of protein kinase C (PKC) by prolonged exposure to 12-O-tetradecanoylphorbol 13-acetate (TPA), blocked v-Src-induced TIS10 expression, but had n...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Prostate cancer is one of the major causes of cancer-related death in the western world. Androgen-deprivation therapy (ADT) for the suppression of androgens binding to the androgen receptor (AR) has been the norm of prostate cancer treatment. Despite early success to suppress prostate tumor growth, ADT eventually fail...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2010.121
更新日期:2010-06-24 00:00:00
abstract::EDD (E3 isolated by differential display), located at chromosome 8q22.3, is the human orthologue of the Drosophila melanogaster tumour suppressor gene 'hyperplastic discs' and encodes a HECT domain E3 ubiquitin protein-ligase. To investigate the possible involvement of EDD in human cancer, several cancers from diverse...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206775
更新日期:2003-08-07 00:00:00
abstract::Drug-resistant clones selected from FR3T3 rat cells after transfer of neo-BPV1 (Bovine Papillomavirus Type 1) DNA constructs became phenotypically transformed (focal transformation, growth in suspension and tumor formation) soon after selection (approximately 5 generations in culture). A frameshift mutation in ORF E5 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract::Transcription factor PAX8 expression is upregulated in several types of cancers. However, little is known about the function of PAX8 in the progression of hepatoma and its regulatory mechanisms. Here, we show that PAX8 silencing inhibits the proliferation and clonogenicity of hepatoma cells and its growth in vivo. The...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0907-2
更新日期:2019-10-01 00:00:00
abstract::KLF6, a ubiquitously expressed Krüppel-like transcription factor, is frequently inactivated in human cancer and has significant roles in cellular proliferation, apoptosis, differentiation and development. A key mechanism of KLF6-mediated growth suppression is through p53-independent transactivation of p21. Several can...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.457
更新日期:2013-09-19 00:00:00