Abstract:
:Platelet-derived growth factor-A (PDGF-A) is a locally produced growth factor in the rat testis secreted by both Sertoli cells and Leydig cells. It has been suggested that PDGF-A may be involved in modulation of testosterone production and may be essential to Leydig cell differentiation, however it is not known at what stage of differentiation PDGF-A begins to be expressed in the cells of Leydig lineage in the postnatal rat testis. Therefore, the objectives of this research were to determine at what postnatal age and in which cell type is PDGF-A first expressed in cells of the adult Leydig cell lineage, and does PDGF-A expression coincide with expression of 3beta-hydroxysteroid dehydrogenase (3beta-HSD), an indicator of steroid hormone synthesis. Male Sprague Dawley rats of postnatal day 1, 7, 9-14, 21, 28, 40, 60, and 90 were used (n=6). Animals were euthanized and their testicles removed, fixed in Bouin's solution, embedded in paraffin, and 5 micrometers sections were prepared. Immunolocalization of PDGF-A and 3beta-HSD was carried out using a peroxidase-streptavidin-biotin method. PDGF-A was first detected in cells of the Leydig cell lineage at postnatal day 10 in progenitor cells, which were surrounding the seminiferous tubules (peritubular). These cells were confirmed to be the progenitor cells and not the mesenchymal or any other spindle-shaped cells in the testis interstitium by immunolocalization of 3beta-HSD and PDGF-A in the cells in adjacent sections of testis tissue from rats of postnatal days 10-14. After postnatal day 10, PDGF-A was continued to be expressed in subsequent cells of the Leydig lineage through day 90 (adult), however, was not present in peritubular mesenchymal precursor cells of the Leydig cell lineage or any other spindle-shaped cells in the testis interstitium at any tested age. These results revealed that PDGF-A first appears in Leydig progenitor cells in the postnatal rat testis at the onset of mesenchymal cell differentiation into progenitor cells at postnatal day 10 and suggest that a functional role(s) of PDGF-A in postnatally differentiated Leydig cells in the rat testis is established at the time of the onset of postnatal Leydig stem cell differentiation. It is suggested that the significance of the first expression of PDGF-A in the Leydig progenitor cells may be associated with inducing cell proliferation and migration of this cell away from the peritubular region during Leydig cell differentiation.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Fecteau KA,Mrkonjich L,Mason JI,Mendis-Handagama SMdoi
10.14670/HH-21.1295subject
Has Abstractpub_date
2006-12-01 00:00:00pages
1295-302issue
12eissn
0213-3911issn
1699-5848journal_volume
21pub_type
杂志文章abstract::CD24 is known to be an important diagnostic and prognostic marker of several major cancers affecting females. We aimed to determine CD24 expression in normal, hyperplastic, and carcinomatous endometrium and its correlation with estrogen and progesterone receptor expression. A total of 271 cases including 62 normal/atr...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-24.309
更新日期:2009-03-01 00:00:00
abstract::The rapid Golgi method, combined with current optical and electronmicroscopical techniques, was used in three central gangliogliomas and in one dysplastic gangliocytoma of the cerebellum to study the morphology of ganglionic cells. Gangliogliomas were composed of bipolar, fusiform and radiate cells with dense core and...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::Gp96 is the endoplasmic reticulum (ER)-resident molecular chaperone, which is involved in the correction of unfolded proteins, in the activation of proteasome-dependent ER-associated degradation of the misfolded proteins, and in activation of the protein translation that modulates polypeptide traffic into the ER. Furt...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.61
更新日期:2013-01-01 00:00:00
abstract::Treatment of neurodegenerative diseases by classical pharmacotherapy is restricted by blood-brain barrier which prevents access to the brain of potentially therapeutic molecules. Recent progress in the knowledge of pathophysiological molecular processes, and in the development of molecular biotechnology have opened th...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-16.883
更新日期:2001-07-01 00:00:00
abstract::Many surgical techniques are employed in the treatment of severe obesity. A main consequence of these techniques is the improvement of type 2 Diabetes mellitus. Ghrelin is a gut hormone released in the gastric fundus and corpus, which has been related to diabetic improvement as mentioned in these papers. Sleeve gastre...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18-200
更新日期:2020-08-01 00:00:00
abstract::The low density lipoprotein receptor-related protein (LRP) 1 is a ubiquitously expressed, versatile cell surface transmembrane receptor involved in embryonic development and adult tissue homeostasis. LRP1 binds and endocytoses a broad spectrum of over 40 ligands identified thus far, including lipoproteins, extracellul...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-746
更新日期:2016-07-01 00:00:00
abstract::A variety of studies have shown that incubation of different tumour cell lines with mistletoe lectins (MLs) in vitro has a marked cytotoxic effect. In the concentration range of low cytotoxicity cell death induced by ML-I is quantitatively due to apoptotic processes. The first events observed being membrane perforatio...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-21.285
更新日期:2006-03-01 00:00:00
abstract::The endocrine cells in the gastrointestinal tract of the musk shrew were studied immunohistochemically. Eleven kinds of endocrine cells, immunoreactive for serotonin, somatostatin, gastrin, cholecistokinin, gastric inhibitory polypeptide, motilin, secretin, neurotensin, pancreatic glucagon, enteroglucagon and bovine p...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::This study evidences the presence of primordial germ cells, in tissue squash preparations and sections from hematological malignancies. Primordial germ cells were identified by their morphology, the intense PAS, PAS-D reaction and presence of calcium-activated neutral proteinase. Primordial germ cells gave rise to nuc...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract::To investigate the matrix metalloproteinase (MMP)-9, (MMP)-13, E-Cadherin and Ki-67 expressions in road pavers' skin chronically exposed to bitumen products in order to contribute to a better understanding of the earlier tissue alteration. Skin punch biopsies from 16 daily exposed workers and a control group were stud...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18-116
更新日期:2019-10-01 00:00:00
abstract::Our study aimed to investigate the association of Pyruvate Kinase isozyme type M2 (PKM2) with radiation resistance in locally advanced cervical squamous cell carcinoma (LACSCC). We retrospectively reviewed 132 female patients who received primary radiation therapy to treat LACSCC at Federation Internationale of Gyneco...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-627
更新日期:2015-11-01 00:00:00
abstract::The nerve fiber distribution in the epidermis of the hairless rat skin was studied light microscopically by means of zinc iodide-osmium tetroxide staining. Two different morphological types of free nerve fiber endings could be detected: clusters of relatively thick nerve fibers stretched up through the spinous layer u...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-15.493
更新日期:2000-04-01 00:00:00
abstract::During endotoxic shock, the liver exerts a lipopolysaccharide (LPS) clearance function with the participation of both parenchymal and sinusoidal cells. Liver damage could be caused by LPS direct action, hypoxia and/or inflammatory mediators released by Kupffer cells. The aim of this study is to establish an experiment...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::E(epithelial)-cadherin is a member of a calcium-dependent family of cell surface glycoproteins involved in cell-cell adhesion and morphogenesis. Catenins are a large family of proteins that connect the cadherins to the cytoskeleton. They are important for cadherin function and for transducing signals involved in speci...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-16.1097
更新日期:2001-10-01 00:00:00
abstract::Detailed characterization of the subdermal model is a significant tool for better understanding of calcification mechanisms occurring in heart valves. In previous ultrastructural investigation on six-week-implantated aortic valve leaflets, modified pre-embedding glutaraldehyde-cuprolinic-blue reactions (GA-CB) enabled...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-22.261
更新日期:2007-03-01 00:00:00
abstract::The salamanderfish, Lepidogalaxias salamandroides (Galaxiidae, Teleostei) is endemic to southwestern Australia and inhabits shallow, freshwater pools which evaporate during the hot summer months. Burrowing into the substrate in response to falling water levels allows these fish to aestivate for extended periods of tim...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-13.1037
更新日期:1998-10-01 00:00:00
abstract::Although testicular germ cell tumors (TGCTs) are relatively uncommon, they are particularly important, as they tend to affect children and young men, the most common tumor being in males aged from 20 to 40 years, and the incidence has been increasing in the last years. TGCTs comprise two major histologic groups: semin...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-29.999
更新日期:2014-08-01 00:00:00
abstract::Ewing-like sarcomas (ELS) are a heterogenous group of tumors that frequently affect pediatric and young adult patients. Accurate classification and distinction from the Ewing sarcoma family of tumor (ESFT) is decisive in patient management. ELS share a significant morphologic, immunohistochemical and clinical overlap ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-792
更新日期:2016-11-01 00:00:00
abstract::Elevated plasma levels of C-reactive protein (CRP) are associated with increased risk of cardiovascular disease. CRP immunoreactive protein is also detected in the lesions of atherosclerosis. However, it is not known whether the CRP contents of atherosclerotic lesions are associated with the initiation and progression...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-27.529
更新日期:2012-04-01 00:00:00
abstract::This report describes the gross, histopathological, immunocytochemical and electron microscopic findings in a cat with systemic cryptococcosis and feline immunodeficiency virus (FIV) infection. Lymphadenopathy and cloudiness of leptomeninges were the major gross findings. Numerous cryptococcal yeasts were found in lym...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Recent studies have shown that papillary renal cell carcinoma (RCC) is clinically and genotypically a distinct entity. Papillary RCCs account for about 10-15% of renal parenchymal neoplasms. Macroscopically, the cut surface is yellow or brown in color and large tumors frequently show cystic change. Hemorrhage and necr...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18.487
更新日期:2003-04-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive cell loss confined mostly to dopaminergic neurons of the substantia nigra. Several factors, including oxidative stress, and decreased activity of complex I mitochondrial respiratory chain, are involved in the degenerative process. Ye...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Human epithelium contains keratin, which is expressed during differentiation. Depending on the target cell type, different types of keratin are expressed, and their alterations seem to represent changes in cell properties. The basal cells of oral epithelium express keratin 5 (K5), K14, K15 and K19, but their alteratio...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-27.949
更新日期:2012-07-01 00:00:00
abstract::Strain injuries of tendons are the most common orthopedic injuries in athletic subjects, be they equine or human. When the tendon is suddenly damaged, an acute inflammatory phase occurs whereas its repetitive overloading may cause chronic injuries. Currently the criteria used for grading injuries are general and subje...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-601
更新日期:2015-08-01 00:00:00
abstract::Bacillus subtilis has been used as a classic model to study biofilm formation and sporulation process. Colonies of wild-type strains usually have a complex external morphology, but the details of their internal architecture are still undisclosed. Since bacterial biofilms fulfill the criteria to be considered tissues, ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-28.1351
更新日期:2013-10-01 00:00:00
abstract::Aneurysmal subarachnoid hemorrhage (SAH) remains a serious cerebrovascular disease. Even if SAH patients survive the initial insults, delayed cerebral ischemia (DCI) may occur at 4 days or later post-SAH. DCI is characteristics of SAH, and is considered to develop by blood breakdown products and inflammatory reactions...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-18-253
更新日期:2020-09-30 00:00:00
abstract::Laminin (LAM) and fibronectin (FI) are regarded as major components of the glomerular extracellular matrix. The aim of this study was to define the distribution of LAM and FI in primary glomerulonephritis (GN) and GN of systemic lupus erythematosus (SLE) and to correlate the type of glomerular disorders with possible ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::We used antisera to pure atrial natriuretic peptide to localise this peptide by immunocytochemistry in rat and human tissue. We showed that both rat and human atrial cardiocytes gave a positive reaction while ventricular cardiocytes were consistently negative. Peripheral islet cells in rat but not in human pancreas al...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::An immunohistochemical study of matrix metalloproteinases (MMP-2 and MMP-9) or gelatinase (gelatinase A and gelatinase B) was performed on the seminal vesicles and ventral prostate of the Libyan jird (Meriones libycus) collected in the Beni-Abbes area during breeding period (spring and early summer), during resting ph...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-25.619
更新日期:2010-05-01 00:00:00
abstract::Peritumoral brain edema (PTBE) is considered to be one of the main biological behaviors of brain glioma. However, the histopathological features of PTBE remain imprecisely defined. We analyzed the histopathological characteristics in the PTBE area of 22 cases of glioma. Microscopically, the pre-existing basic structur...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-607
更新日期:2015-09-01 00:00:00