Low-intensity ultrasound stimulation enhances chondrogenic differentiation in alginate culture of mesenchymal stem cells.

Abstract:

:Mesenchymal stem cells (MSCs) are regarded as a potential autologous source for cartilage repair, because they can differentiate into chondrocytes by transforming growth factor-beta (TGF-beta) treatment under the 3-dimensional (3-D) culture condition. However, more efficient and versatile methods for chondrogenic differentiation of MSCs are still in demand for its clinical application. Recently, low-intensity ultrasound (LIUS) was shown to enhance fracture healing in vitro and induce chondrogenesis of MSCs in vitro. In this study, we investigated the effects of LIUS on the chondrogenesis of rabbit MSCs (rMSCs) in a 3-D alginate culture and on the maintenance of chondrogenic phenotypes after replating them on a monolayer culture. The LIUS treatment of rMSCs increased: (i) the matrix formation; (ii) the expression of chondrogenic markers such as collagen type II, aggrecan, and Sox-9; (iii) the expression of tissue inhibitor of metalloprotease-2 implicated in the integrity of cartilage matrix; and (iv) the capacity to maintain the chondrogenic phenotypes in a monolayer culture. Notably, LIUS effects were clearly shown even without TGF-beta treatment. These results suggest that LIUS treatment could be an efficient and cost-effective method to induce chondrogenic differentiation of MSCs in vitro for cartilage tissue engineering.

journal_name

Artif Organs

journal_title

Artificial organs

authors

Lee HJ,Choi BH,Min BH,Son YS,Park SR

doi

10.1111/j.1525-1594.2006.00288.x

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

707-15

issue

9

eissn

0160-564X

issn

1525-1594

pii

AOR288

journal_volume

30

pub_type

杂志文章
  • Low molecular weight heparin versus unfractioned heparin for anticoagulation during perioperative extracorporeal membrane oxygenation: A single center experience in 102 lung transplant patients.

    abstract::Extracorporeal membrane oxygenation (ECMO) is gaining importance in the perioperative management of lung transplant patients. To date, the ideal substance for anticoagulation of ECMO patients is still a matter of debate. In this study, we describe our experience with the use of low molecular weight heparin (LMWH) in c...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/aor.13642

    authors: Gratz J,Pausch A,Schaden E,Baierl A,Jaksch P,Erhart F,Hoetzenecker K,Wiegele M

    更新日期:2020-06-01 00:00:00

  • The microsystems based visual prosthesis for optic nerve stimulation.

    abstract::The microsystems based visual prosthesis (MiViP) visual prosthesis generates visual perceptions well below safety and stimulator saturation limits. These perceptions, called phosphenes, are of reasonably small size and are broadly distributed in the visual field. They can thus be used to convey useful visual informati...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1046/j.1525-1594.2002.06939.x

    authors: Delbeke J,Wanet-Defalque MC,Gérard B,Troosters M,Michaux G,Veraart C

    更新日期:2002-03-01 00:00:00

  • Effect of a balanced biventricular bypass system on left ventricular energies.

    abstract::Left dominant biventricular failure is a common type of heart failure after cardiac surgery. We developed a biventricular bypass (BVB) system for treatment of postcardiotomy ventricular failure, and we previously reported that the clinical results of the BVB system were superior to those obtained with venoarterial byp...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1046/j.1525-1594.1998.06015.x

    authors: Kodera K,Kitamura M,Shibuya M,Kurihara H,Koyanagi H

    更新日期:1998-09-01 00:00:00

  • Pseudoendothelium formation in expanded polytetrafluoroethylene grafts implanted in the inferior vena cava of normal and defibrinogenated dogs.

    abstract::The outgrowth of endothelial cells in an expanded polytetrafluoroethylene graft implanted in the canine inferior vena cava was studied by scanning electron microscopy. The adjacent endothelial cells of the vena cava started to invade the anastomotic area three days after the implantation and reached approximately the ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1981.tb04020.x

    authors: Ishimaru S,Berglin E,Hansson HA,Teger-Nilsson AC,William-Olsson G

    更新日期:1981-11-01 00:00:00

  • A bedside micromethod for the determination of total carbon dioxide in biological fluids.

    abstract::The quality control of bicarbonate hemodialysis requires the determination of TCO2, either in blood or in dialysate. The use of the M + D TCO2 kit, a bedside micromethod, gave the authors an economical and reliable solution to this problem. ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1985.tb04404.x

    authors: Canaud B,Araujo A,Nguyen QV,Flavier JL,Mion C

    更新日期:1985-11-01 00:00:00

  • Glucose-insulin kinetics of a bioartificial pancreas made of an AN69 hydrogel hollow fiber containing porcine islets and implanted in diabetic mice.

    abstract::The goal of this study was to determine whether porcine islets encapsulated in hollow fibers made of AN69 copolymer can correct hyperglycemia in diabetic mice and provide normal tolerance to a glucose challenge. In vitro perifusion of hollow fibers demonstrated the rapid kinetics of insulin release in response to gluc...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1046/j.1525-1594.1998.05087.x

    authors: Delaunay C,Darquy S,Honiger J,Capron F,Rouault C,Reach G

    更新日期:1998-04-01 00:00:00

  • Bulk mass transport limitations during high-flux hemodialysis.

    abstract::Solute clearance during high-flux hemodialysis is determined by the combined effects of solute convection and diffusion in the membrane, blood, and dialysate. Previous studies have focused almost entirely on the membrane transport phenomena, largely ignoring the potential contribution from bulk mass transport across t...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1993.tb00403.x

    authors: Zydney AL

    更新日期:1993-11-01 00:00:00

  • Cryopreservation of fibroblasts immobilized within a porous scaffold: effects of preculture and collagen coating of scaffold on performance of three-dimensional cryopreservation.

    abstract::As a preliminary investigation to establish a cryopreservation method suited for bioartificial livers (BALs), three-dimensional (3-D) cryopreservation experiments with fibroblasts were performed, in which the cells were firstly seeded into a porous scaffold, and the scaffold containing the cells was then cryopreserved...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.2009.00933.x

    authors: Miyoshi H,Ehashi T,Ohshima N,Jagawa A

    更新日期:2010-07-01 00:00:00

  • Engineering of vascular grafts with genetically modified bone marrow mesenchymal stem cells on poly (propylene carbonate) graft.

    abstract::Bone marrow mesenchymal stem cells (MSCs) have demonstrated their pluripotency to differentiate into different cell lineages and may be an alternative cell source for vascular tissue engineering. The objective of this study is to create small diameter vessels by seeding and culture of genetically modified MSCs onto a ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.2006.00322.x

    authors: Zhang J,Qi H,Wang H,Hu P,Ou L,Guo S,Li J,Che Y,Yu Y,Kong D

    更新日期:2006-12-01 00:00:00

  • Proof of principle of a novel co-pulsating intra-ventricular membrane pump.

    abstract::In this proof of principle study, we investigated the effectiveness and safety of hemodynamic support with the Intra-Ventricular Membrane Pump (IVMP). The IVMP was implanted into the apex of the left ventricle. Hemodynamic assessment was performed in six ex vivo beating porcine hearts (PhysioHeart platform). The cardi...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/aor.13757

    authors: van Dort DIM,Thannhauser J,Gommans FDH,Ten Cate TJ,Duncker DJ,Suryapranata H,Morshuis WJ,Geuzebroek GSC

    更新日期:2020-12-01 00:00:00

  • Electrochemical and Electrophysiological Performance of Platinum Electrodes Within the Ninety-Nine-Electrode Stimulating Nerve Cuff.

    abstract::The trend in neural prostheses using selective nerve stimulation for electrical stimulation therapies is headed toward single-part systems having a large number of working electrodes (WEs), each of which selectively stimulate neural tissue or record neural response (NR). The present article reviews the electrochemical...

    journal_title:Artificial organs

    pub_type: 杂志文章,评审

    doi:10.1111/aor.12625

    authors: Pečlin P,Mehle A,Karpe B,Rozman J

    更新日期:2015-10-01 00:00:00

  • Quantification of the secondary flow in a radial coupled centrifugal blood pump based on particle tracking velocimetry.

    abstract::Secondary flow in the centrifugal blood pump helps to enhance the washout effect and to minimize thrombus formation. On the other hand, it has an adverse effect on pump efficiency. Excessive secondary flow may induce hemolytic effects. Understanding the secondary flow is thus important to the design of a compact, effi...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.2004.29012.x

    authors: Watanabe N,Masuda T,Iida T,Kataoka H,Fujimoto T,Takatani S

    更新日期:2005-01-01 00:00:00

  • A pilot study of piracetam in cuprophan hemodialysis.

    abstract::Twelve patients, 8 male and 4 female, aged from 26 to 70 years were studied. They were on hemodialysis (HD) 4 h three times weekly with cuprophan hollow fiber dialyzers (Gambro 120M) and dialysate containing 35 mEq/L of acetate. The study compares two random HD sessions on each patient. Sixty minutes prior to one rand...

    journal_title:Artificial organs

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1525-1594.1989.tb01551.x

    authors: Grekas D,Alivanis P,Karamouzis M,Pyrpasopoulos M,Tourkantonis A

    更新日期:1989-10-01 00:00:00

  • Can We Develop a Nonpulsatile Permanent Rotary Blood Pump? Yes, We Can.

    abstract::For many years, it was thought that nonpulsatile perfusion produced physiological and circulatory abnormalities. Since 1977, Yukihiko Nosé and his colleagues have challenged this misconception. Toward that end, they did show that if a 20% higher blood flow uses more than that required for a pulsatile blood pump, then ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1996.tb04466.x

    authors: Nosé Y,Kawahito K,Nakazawa T

    更新日期:1996-05-01 00:00:00

  • An In Vitro Feasibility Study of the Influence of Configurations and Leaflet Thickness on the Hydrodynamics of Deformed Transcatheter Aortic Valve.

    abstract::Clinically, the percutaneous transcatheter aortic valve (TAV) has been reported to be deformed in a noncircular configuration after its implant. The deformation is universal and various, and it leads to serious leakage and durability problems. Even in the same deformation, the leaflets made in different tissue thickne...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/aor.12833

    authors: Feng W,Yang X,Liu Y,Fan Y

    更新日期:2017-08-01 00:00:00

  • Thrombogenicity of deflated intraaortic balloon: impact of heparin coating.

    abstract::In a calf model, 3 standard and 3 heparin coated intraaortic balloons (IAB) were inserted and left deflated in the descending aorta during 20 min to simulate balloon rupture. At the end of the experiment, the IAB were examined, and 3 samples of each were collected for scanning electron microscopy (SEM) analysis. None ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1046/j.1525-1594.1999.06228.x

    authors: Mueller XM,Tevaearai HT,Hayoz D,von Segesser LK

    更新日期:1999-02-01 00:00:00

  • A new smartphone-based method for wound area measurement.

    abstract::Proper wound healing can be assessed by monitoring the wound surface area. Its reduction by 10 or 50% should be achieved after 1 or 4 weeks, respectively, from the start of the applied therapy. There are various methods of wound area measurement, which differ in terms of the cost of the devices and their accuracy. Thi...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/aor.12169

    authors: Foltynski P,Ladyzynski P,Wojcicki JM

    更新日期:2014-04-01 00:00:00

  • Internal jugular vein cannulation with two silicone rubber catheters: a new and safe temporary vascular access for hemodialysis. Thirty months' experience.

    abstract::Temporary and immediately usable vascular access is a vital need in treating uremic patients. Subclavian vein cannulation, although a major progress, has been associated with significant morbidity and mortality. Accordingly, for the last 2.5 years the authors have been developing an alternative technique consisting of...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1986.tb02587.x

    authors: Canaud B,Beraud JJ,Joyeux H,Mion C

    更新日期:1986-10-01 00:00:00

  • Can the human lumbar posterior columns be stimulated by transcutaneous spinal cord stimulation? A modeling study.

    abstract::Stimulation of different spinal cord segments in humans is a widely developed clinical practice for modification of pain, altered sensation, and movement. The human lumbar cord has become a target for modification of motor control by epidural and, more recently, by transcutaneous spinal cord stimulation. Posterior col...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.2011.01213.x

    authors: Danner SM,Hofstoetter US,Ladenbauer J,Rattay F,Minassian K

    更新日期:2011-03-01 00:00:00

  • A preliminary study of microcapsule suspension for hemolysis evaluation of artificial organs.

    abstract::A microcapsule suspension, a substitute for animal blood in hemolysis tests, has been developed for evaluation of the absolute hemolytic properties of circulatory artificial organs. The microcapsule suspension was made by dispersing microcapsule slurry into an ethylene glycol sodium chloride solution. The microcapsule...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1046/j.1525-1594.1999.06316.x

    authors: Maruyama O,Yamane T,Tsunemoto N,Nishida M,Tsutsui T,Jikuya T

    更新日期:1999-03-01 00:00:00

  • Coronary artery bypass grafting for dialysis patients: usefulness of multiarterial bypass.

    abstract::This study assessed the efficacy of multiarterial bypass in coronary artery bypass grafting (CABG) in dialysis patients. Eighty dialysis patients who underwent CABG were divided into 2 groups. Group A consisted of 38 patients in whom the left internal thoracic artery and additional saphenous vein graft (SVG) had been ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:

    authors: Nakayama Y,Sakata R,Ura M

    更新日期:2001-04-01 00:00:00

  • Device exchange versus nonexchange modalities in left ventricular assist device-specific infections: A systematic review and meta-analysis.

    abstract::No standardized treatment algorithm exists for the management of continuous-flow left ventricular assist device (CF-LVAD)-specific infections. The aim of this systematic review and meta-analysis was to compare the outcomes of CF-LVAD-specific infections as managed by device exchange to other treatment modalities not i...

    journal_title:Artificial organs

    pub_type: 杂志文章,meta分析

    doi:10.1111/aor.13378

    authors: Bauer TM,Choi JH,Luc JGY,Weber MP,Moncho Escrivá E,Patel S,Maynes EJ,Boyle AJ,Samuels LE,Entwistle JW,Morris RJ,Massey HT,Tchantchaleishvili V

    更新日期:2019-05-01 00:00:00

  • Atrial versus ventricular cannulation for a rotary ventricular assist device.

    abstract::The ventricular assist device inflow cannulation site is the primary interface between the device and the patient. Connecting these cannulae to either atria or ventricles induces major changes in flow dynamics; however, there are little data available on precise quantification of these changes. The objective of this i...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.2010.01093.x

    authors: Timms D,Gregory S,Hsu PL,Thomson B,Pearcy M,McNeil K,Fraser J,Steinseifer U

    更新日期:2010-09-01 00:00:00

  • Central Extracorporeal Life Support With Left Ventricular Decompression to Berlin Heart Excor: A Reliable "Bridge to Bridge" Strategy in Crash and Burn Patients.

    abstract::The aim of this study was to compare patients with severe biventricular heart failure who underwent Berlin Heart Excor implantation with (cardiogenic shock [CS] status) or without the need for preoperative extracorporeal life support (ECLS) as a bridge to long-term device. A total of 40 consecutive patients with sever...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/aor.12792

    authors: Weymann A,Farag M,Sabashnikov A,Fatullayev J,Zeriouh M,Schmack B,Arif R,Müller F,Alt C,Raake P,Prakash Patil N,Popov AF,Rüdiger Simon A,Karck M,Ruhparwar A,Heidelberg-Cologne-Harefield Cardiothoracic Transplantation & Mecha

    更新日期:2017-06-01 00:00:00

  • Long-term performance of homograft and autograft valves.

    abstract::Five hundred and eighty homograft valves have been inserted into the aortic position as isolated valve replacements, and followed up for 7--14 years. After ten years 90% of frozen homografts, 80% of autografts and 70% of freeze-dried homografts were free of valve-related death. There was only one embolism reported giv...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1980.tb03894.x

    authors: Bodnar E,Wain WH,Martelli V,Ross DN

    更新日期:1980-02-01 00:00:00

  • New immunosuppressive proteins (KX-2, -4, and -5) induced by liver transplantation.

    abstract::The presence in rat serum of three novel immunosuppressive proteins is associated with induction of tolerance after liver grafting. One of these, KX-5 (40 kilodalton molecular weight) is present 40-100 days after grafting of DA liver into PVG, a combination in which liver grafts are not rejected; two others, KX-2 (87 ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1996.tb00646.x

    authors: Kamada N

    更新日期:1996-10-01 00:00:00

  • Nitric oxide added to the sweep gas of the oxygenator during cardiopulmonary bypass in infants: A pilot randomized controlled trial.

    abstract::Our objective was to assess the effect of nitric oxide added to the sweep gas of the oxygenator during cardiopulmonary bypass (CPB) in infants on platelet count, platelet function, clinical outcomes, and safety. A randomized, double-blinded, placebo-controlled clinical trial in infants less than a year of age undergoi...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/aor.13788

    authors: Niebler RA,Chiang-Ching H,Daley K,Janecke R,Jobe SM,Mitchell ME,Varner C,Woods K,Scott JP

    更新日期:2020-08-01 00:00:00

  • Cooling device for bradycardia based on Peltier element for accurate anastomosis of off-pump coronary artery bypass grafting.

    abstract::Upon introducing off-pump coronary artery bypass grafting (CABG), the indications for CABG were expanded to include patients who previously had no operative indications. For accurate anastomosis, various devices and methods have been developed. Bradycardia is easily induced by drug administration. However, this method...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1046/j.1525-1594.2002.07087.x

    authors: Kuniyoshi Y,Koja K,Miyagi K,Shimoji M,Uezu T,Arakaki K,Yamashiro S,Mabuni K,Senaha S

    更新日期:2002-10-01 00:00:00

  • Eosinophilia in hemodialysis patients.

    abstract::Thirteen percent of 99 patients undergoing maintenance hemodialysis showed an increased predialysis peripheral blood eosinophil count (mean value of 828 cells/mm3, range 410-2,710). Patients with eosinophilia were predominantly male and evidenced more frequent elevation of total plasma IgE levels. Otherwise, no differ...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1987.tb02635.x

    authors: Friedberg M,Joffe P,Nielsen B,Nielsen LP

    更新日期:1987-04-01 00:00:00

  • Flow pattern analysis of the Baylor total artificial heart.

    abstract::To obtain an optimal design of the left blood chamber of the total artificial heart (TAH), flow visualization studies were performed. Information on velocities in critical areas of the left chamber was gathered using sectional pulsed laser light. The flow patterns on the entire pumping duration were photographed frame...

    journal_title:Artificial organs

    pub_type: 杂志文章

    doi:10.1111/j.1525-1594.1994.tb03345.x

    authors: Meier D,Wernicke JT,Orime Y,Takatani S,Tasai K,Damm G,Naito K,Mizuguchi K,Makinouchi K,Glueck J

    更新日期:1994-12-01 00:00:00