Abstract:
BACKGROUND:Human intestinal epithelial cells (IECs) secrete the chemokine CCL20 in response to infection by various enteropathogenic bacteria or exposure to bacterial flagellin. CCL20 recruits immature dendritic cells and lymphocytes to target sites. Here we investigated IEC responses to various pathogenic and commensal bacteria as well as the modulatory effects of commensal bacteria on pathogen-induced CCL20 secretion. HT-29 human IECs were incubated with commensal bacteria (Bifidobacterium infantis or Lactobacillus salivarius), or with Salmonella typhimurium, its flagellin, Clostridium difficile, Mycobacterium paratuberculosis, or Mycobacterium smegmatis for varying times. In some studies, HT-29 cells were pre-treated with a commensal strain for 2 hr prior to infection or flagellin stimulation. CCL20 and interleukin (IL)-8 secretion and nuclear factor (NF)-kappaB activation were measured using enzyme-linked immunosorbent assays. RESULTS:Compared to untreated cells, S. typhimurium, C. difficile, M. paratuberculosis, and flagellin activated NF-kappaB and stimulated significant secretion of CCL20 and IL-8 by HT-29 cells. Conversely, B. infantis, L. salivarius or M. smegmatis did not activate NF-kappaB or augment CCL20 or IL-8 production. Treatment with B. infantis, but not L. salivarius, dose-dependently inhibited the baseline secretion of CCL20. In cells pre-treated with B. infantis, C. difficile-, S. typhimurium-, and flagellin-induced CCL20 were significantly attenuated. B. infantis did not limit M. Paratuberculosis-induced CCL20 secretion. CONCLUSION:This study is the first to demonstrate that a commensal strain can attenuate CCL20 secretion in HT-29 IECs. Collectively, the data indicate that M. paratuberculosis may mediate mucosal damage and that B. infantis can exert immunomodulatory effects on IECs that mediate host responses to flagellin and flagellated enteric pathogens.
journal_name
BMC Immunoljournal_title
BMC immunologyauthors
Sibartie S,O'Hara AM,Ryan J,Fanning A,O'Mahony J,O'Neill S,Sheil B,O'Mahony L,Shanahan Fdoi
10.1186/1471-2172-10-54subject
Has Abstractpub_date
2009-10-08 00:00:00pages
54issn
1471-2172pii
1471-2172-10-54journal_volume
10pub_type
杂志文章相关文献
BMC IMMUNOLOGY文献大全abstract:BACKGROUND:Down syndrome (DS) is the most common syndromic immunodeficiency with an increased risk of infection, mortality from sepsis, and autoinflammation. Innate immune function is altered in DS and therefore we examined responses in CD11b and Toll like receptor 4 (TLR-4), which are important immune cell surface mar...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0270-z
更新日期:2018-11-03 00:00:00
abstract:BACKGROUND:Allergic skin inflammation such as atopic dermatitis (AD), which is characterized by pruritus and inflammation, is regulated partly through the activity of regulatory T cells (Tregs). Tregs play key roles in the immune response by preventing or suppressing the differentiation, proliferation and function of v...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-13-44
更新日期:2012-08-09 00:00:00
abstract:BACKGROUND:Tuberculous pleural effusion (TPE) is one of the most common forms of extrapulmonary tuberculosis. Because most studies of TPE focused on the pleural space, little information regarding lung parenchyma is available. We therefore aimed to investigate immune responses in the lung parenchyma of TPE patients wit...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-13-45
更新日期:2012-08-13 00:00:00
abstract:BACKGROUND:An ideal tumor vaccine should activate both effector and memory immune response against tumor-specific antigens. Beside the CD8+ T cells that play a central role in the generation of a protective immune response and of long-term memory, dendritic cells (DCs) are important for the induction, coordination and ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-45
更新日期:2010-09-13 00:00:00
abstract:BACKGROUND:Recognition of pathogens by dendritic cells (DCs) through interaction with pattern recognition receptors, including Toll like receptors (TLR), is crucial for the initiation of appropriate polarized T helper (Th) cell responses. Yet, the characteristics and differences in molecular profiles of DCs with differ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-9
更新日期:2009-02-04 00:00:00
abstract:BACKGROUND:Immunophenotyping of blood lymphocytes is an essential tool to evaluate the immune function of patients with immunodeficiency or autoimmunity. Predominately identified CD4+T cell subsets, Th1, Th2, Th17, as well as regulatory T (Treg) cells, play crucial roles in several immunological and pathological condit...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-00374-9
更新日期:2020-08-03 00:00:00
abstract:BACKGROUND:The need for rapid and accurate comparison of panels of adjuvanted vaccine formulations and subsequent rational down selection, presents several challenges for modern vaccine development. Here we describe a method which may enable vaccine and adjuvant developers to compare antigen/adjuvant combinations in a ...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0245-0
更新日期:2018-01-31 00:00:00
abstract:BACKGROUND:Eosinophils are innate immune cells present in the intestine during steady state conditions. An intestinal eosinophilia is a hallmark of many infections and an accumulation of eosinophils is also observed in the intestine during inflammatory disorders. Classically the function of eosinophils has been associa...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-016-0153-0
更新日期:2016-05-31 00:00:00
abstract:AIM:Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-00390-9
更新日期:2020-11-18 00:00:00
abstract:BACKGROUND:While tumor necrosis factor alpha (TNF-α) inhibitors (TNFi) and other biologics are very effective against autoimmune diseases, they can also cause infectious diseases. Therefore, it is important to clarify whether the TNFi sometimes used to treat patients with rheumatoid arthritis (RA) complicated with huma...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-017-0191-2
更新日期:2017-02-03 00:00:00
abstract:BACKGROUND:Severe acute respiratory syndrome (SARS) is an emerging infectious disease caused by the novel coronavirus SARS-CoV. The T cell epitopes of the SARS CoV spike protein are well known, but no systematic evaluation of the functional and structural roles of each residue has been reported for these antigenic epit...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-13-50
更新日期:2012-09-10 00:00:00
abstract:BACKGROUND:Cryopreservation of peripheral blood mononuclear cells has been used to preserve and standardize immunologic measurements for multicenter studies, however, effects of cryopreservation on cytokine responses are incompletely understood. In designing immunologic studies for a new multicenter birth cohort study ...
journal_title:BMC immunology
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2172-7-29
更新日期:2006-12-12 00:00:00
abstract:BACKGROUND:Toll-like receptor 2 (TLR2) plays a critical role in mediating inflammatory/immune responses against bacterial pathogens in lung. Streptococcus pneumoniae (S. pneumoniae) and nontypeable Haemophilus influenzae (NTHi) were previously reported to synergize with each other to induce inflammatory responses. Desp...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-40
更新日期:2008-07-29 00:00:00
abstract:BACKGROUND:We sought to compare Pneumovax®23 responses in adults with subnormal IgG subclass concentrations. We studied adults with normal total IgG, frequent/severe respiratory infection, and subnormal IgG1, IgG3, or IgG1 + IgG3 before and after Pneumovax®23. We defined response as serotype-specific IgG > 1.3 μg/mL an...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0310-3
更新日期:2019-08-20 00:00:00
abstract:BACKGROUND:The functional immaturity of T cells contributes to the susceptibility of neonates to infections and the less severe graft-versus-host disease associated with cord blood (CB) transplantation. We have previously reported that insulin-like growth factor - I (IGF-I) promotes the phytohaemagglutinin (PHA)-induce...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-74
更新日期:2008-12-17 00:00:00
abstract:BACKGROUND:Recent studies have shown that early growth response 2 (EGR2) is highly induced in activated T cells and regulates T cell functions. In normal C57BL/6 (B6) mice, deletion of EGR2 in lymphocytes results in the development of lupus-like systemic autoimmune disease, which implies indirectly an autoimmune protec...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-020-00370-z
更新日期:2020-07-09 00:00:00
abstract:BACKGROUND:Egg white must provide nutrients and protection to the developing avian embryo. One way in which this is achieved is an arsenal of antimicrobial proteins and peptides which are essentially extensions of the innate immune system. Gallin is a recently identified member of a family of peptides that are found in...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-11-12
更新日期:2010-03-12 00:00:00
abstract:BACKGROUND:Regulatory CD4 T cells (Tregs) are critical in maintaining the homeostasis of the immune system. Quantitative or phenotypic alterations and functional impairment of Tregs have been associated with the development of pathologies including those of the central nervous system. Individuals with HIV-1/HTLV-1 co-i...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-015-0116-x
更新日期:2015-09-02 00:00:00
abstract:BACKGROUND:Tuberculosis (TB) remains a serious human health problem that affects millions of people in the world. Understanding the biology of Mycobacterium tuberculosis (Mtb) is essential for tackling this devastating disease. Mtb possesses a very complex cell envelope containing a variety of lipid components that par...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-015-0102-3
更新日期:2015-06-23 00:00:00
abstract:BACKGROUND:The lymphatic vascular pattern in the head of mice has rarely been studied, due to problems of sectioning and immunostaining of complex bony structures. Therefore, the association of head lymphoid tissues with the lymphatics has remained unknown although the mouse is the most often used species in immunology...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-018-0242-3
更新日期:2018-01-25 00:00:00
abstract:BACKGROUND:Most HIV-infected subjects exhibit a progressive rise in CD4 T-cell counts after initiation of highly active antiretroviral therapy (HAART). However, a subset of individuals exhibit very poor CD4 T-cell recovery despite effective control of HIV-RNA viraemia. We evaluated CD4 T-cell proliferation among subopt...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-14-26
更新日期:2013-06-20 00:00:00
abstract:BACKGROUND:In genetically modified (GM) crops there is a risk that the inserted genes may introduce new allergens and/or adjuvants into the food and feed chain. The MON810 maize, expressing the insecticidal Cry1Ab toxin, is grown in many countries worldwide. In animal models, intranasal and intraperitoneal immunisation...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-016-0148-x
更新日期:2016-05-04 00:00:00
abstract:BACKGROUND:The gram-negative bacterium Bordetella pertussis is an important causative agent of pertussis, an infectious disease of the respiratory tract. After introduction of whole-cell vaccines (wP) in the 1950's, pertussis incidence has decreased significantly. Because wP were found to be reactogenic, in most develo...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-21
更新日期:2008-05-22 00:00:00
abstract:BACKGROUND:We have previously reported that interleukin-10 (IL-10) deficient dendritic cells (DCs) are potent antigen presenting cells that induced elevated protective immunity against Chlamydia. To further investigate the molecular and biochemical mechanism underlying the superior immunostimulatory property of IL-10 d...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-017-0212-1
更新日期:2017-05-19 00:00:00
abstract:BACKGROUND:Regulatory T cells (Treg) play a crucial role in maintaining immune homeostasis and self-tolerance. The immune suppressive effects of Tregs should however be limited in case effective immunity is required against pathogens or cancer cells. We previously found that the Toll-like receptor 2 (TLR2) agonist, Pam...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-12-23
更新日期:2011-03-24 00:00:00
abstract:BACKGROUND:Proinflammatory genes are highly expressed in several metabolic disorders associated with obesity. But it is not clarified whether gene expression levels and downstream inflammatory markers are related to the metabolic state or the presence of obesity. Hence, the present study aimed to compare Toll-Like Rece...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-019-0303-2
更新日期:2019-07-04 00:00:00
abstract:BACKGROUND:Pattern recognition receptors (PRRs) for double-stranded RNA (dsRNA) are components of innate immunity that recognize the presence of viral infection and initiate efficient defense mechanisms. In addition to previously well-characterized signaling pathways that are mediated by PKR and TLR3, new intracellular...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-12-15
更新日期:2011-02-10 00:00:00
abstract:BACKGROUND:Class I major histocompatibility complex (MHC) molecules bind, and present to T cells, short peptides derived from intracellular processing of proteins. The peptide repertoire of a specific molecule is to a large extent determined by the molecular structure accommodating so-called main anchor positions of th...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-9-1
更新日期:2008-01-22 00:00:00
abstract:BACKGROUND:To understand the role of genetic factors on chromosome 1 in the regulation of spontaneous arthritis in mice deficient in IL-1 receptor antagonist protein (IL_1RA), we previously used speed congenic breeding to transfer the QTL region from DBA/1(-/-) mice that are resistant to spontaneous arthritis into BALB...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/s12865-014-0057-9
更新日期:2014-12-09 00:00:00
abstract:BACKGROUND:It has been recognized that phospholipase A2 (PLA2) is a crucial component of snake venom, which contributes greatly to snake venom induced inflammation in man. However, the mechanisms through which N49 PLA2 provoke inflammation remain unclear. Recently, a N49 PLA2, TM-N49 from Protobothrops mucrosquamatus c...
journal_title:BMC immunology
pub_type: 杂志文章
doi:10.1186/1471-2172-10-21
更新日期:2009-04-28 00:00:00