Abstract：
:Orexin-A (OX-A) and -B (OX-B) peptides present in the hippocampus are considered to be exclusively contained in fibers arising from hypothalamus neurons, which were established as the only source of orexins (OXs). Because OX-A is known to exert excitatory actions in the hippocampus, we hypothesized that the level of OXs targeted toward the hippocampus may be increased following status-epilepticus (SE)-induced epileptogenesis in the rat pilocarpine model of temporal lobe epilepsy. We found that tissue concentration of prepro-OX mRNA, which encodes for both peptides, rapidly decreased in the hypothalamus of rats having experienced pilocarpine-induced SE (Pilo-SE) followed by a reduced density of OX-A and OX-B immunopositive fibers arising from these neurons. By contrast, it was unexpected to detect within the hippocampus the presence of prepro-OX mRNA in basal conditions and to evidence its up-regulation during the 1- to 3-day period following Pilo-SE. The number of prepro-OX mRNA copies determined by real-time RT-PCR was approximately 50-fold lower in the hippocampus than that in the hypothalamus, precluding the use of in situ hybridization to localize the cells which synthesize the transcript within the hippocampus. The increase in prepro-OX mRNA level within the hippocampus was accompanied by the detection of OX-B-like immunoreactivity 2-3 days post-SE, not only in pyramidal neurons, granule cells and cell bodies resembling interneurons, but also in some astrocytes scattered throughout the hippocampus. The present data suggest that the gene encoding OXs can be activated in the hippocampus, which may play a role in the pathogenesis of epilepsy.
journal_name
Brain Resjournal_title
Brain researchauthors
Morales A,Bonnet C,Bourgoin N,Touvier T,Nadam J,Laglaine A,Navarro F,Moulin C,Georges B,Pequignot JM,Bezin Ldoi
10.1016/j.brainres.2006.06.075subject
Has Abstractpub_date
2006-09-13 00:00:00pages
164-75issue
1eissn
0006-8993issn
1872-6240pii
S0006-8993(06)01793-8journal_volume
1109pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Our previous studies demonstrated that Austrolebias charrua annual fish is an excellent model to study adult brain cell proliferation and neurogenesis due to the presence of active and fast neurogenesis in several regions during its short lifespan. Our main goal was to identify and localize the cells that compose the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.08.003
更新日期：2017-10-15 00:00:00
abstract：:The effect of a single subcutaneous (s.c.) injection of the ultrapotent capsaicin analogue resiniferatoxin (RTX) on responses of adult rats to noxious thermal and mechanical stimulation was examined. The effects of RTX treatment on the nociceptive flexor reflex and activity-dependent increase in spinal excitability af...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01444-8
更新日期：1997-03-28 00:00:00
abstract：:Met-enkephalin-like immunoreactive material (ME-LI) in the neurointermediate lobe of rat pituitary was dramatically altered by the long-term administration of drugs altering dopamine receptor activity. Treatment with the dopamine receptor antagonist haloperidol resulted in significant increases of ME-LI and treatment ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90586-4
更新日期：1985-05-13 00:00:00
abstract：:Activation of phospholipase C (PLC) coupled to phosphoinositide (PtdIns) hydrolysis occurs through one of the two pathways. One of the major pathways for the neurotransmitter signaling involves phosphoinositide (PtdIns) specific and G-protein dependent PLC-beta, which stimulates the formation of inositol triphosphate ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01272-9
更新日期：1996-02-05 00:00:00
abstract：:The ability to adapt a variety of motor acts to compensate for persistent natural or artificially induced errors in movement accuracy requires the cerebellum. For adaptation of the rapid shifts in the direction of gaze called saccades, the oculomotor vermis (OMV) of the cerebellum must be intact. We disrupted the neur...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.05.027
更新日期：2011-07-15 00:00:00
abstract：:Unilateral destruction of the entorhinal area in the rat results in the proliferation of a pathway from the surviving contralateral entorhinal area to the fascia dentata denervated by the lesions (the crossed temporodentate pathway). The present study analyzes the point of entry of these reinnervating fibers into the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90464-3
更新日期：1980-02-10 00:00:00
abstract：:A quantitative study of Wallerian degeneration was carried out on teased fibres. The breakdown into ovoids was used as the criterion of degeneration. The fragmentation of fibres begins near the point of nerve interrruption and spreads along the unbranched parts of axons at velocities correlated with fibre diameter and...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90841-1
更新日期：1977-07-08 00:00:00
abstract：:We cloned the genes for the mouse homologue of the neuromedin B receptor (NMB-R) and the bombesin receptor subtype 3 (BRS-3). Both receptor genes consist of three exons with well-conserved intron-exon borders. Although the NMB-R gene spans more than 10 kb, the BRS-3 gene spans only about 4 kb. Comparison of the mouse ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00380-6
更新日期：1997-07-11 00:00:00
abstract：:The hippocampal formation is a highly plastic brain region that is sensitive to stress. It receives extensive noradrenergic projections, and noradrenaline is released in the hippocampus in response to stressor exposure. The hippocampus expresses particularly high levels of the alpha(1D) adrenergic receptor (ADR) and w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.01.084
更新日期：2010-04-06 00:00:00
abstract：:The N400 is an event-related potential (ERP) component that is elicited by semantically meaningful stimuli; one of its defining characteristics is that it is amplified for sentence completions that are semantically unexpected or incongruous with the preceding context. Some prior reports using visual sentence reading p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.07.099
更新日期：2010-10-08 00:00:00
abstract：:In order to determine the origin of the catecholamine innervation of the rat piriform cortex (PC), we combined retrograde transport of the B subunit of the cholera toxin (CTb) with tyrosine hydroxylase (TH) immunohistochemistry. A substantial number of CTb retrogradely labeled cells was found in the parabrachial pigme...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01279-6
更新日期：1996-02-26 00:00:00
abstract：:Cell number and proliferation of reactive astrocytes were studied quantitatively in the stabbed cerebral cortex of adult mice, using immunohistochemistry for glial fibrillary acidic protein (GFAP) and [3H]thymidine autoradiography. GFAP-positive astrocytes increased in cell number gradually from 24 to 96 h after stabb...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90757-3
更新日期：1988-06-07 00:00:00
abstract：:Synaptosomes assemble an equatorial coil of microtubules during incubation at 37 degrees C. Stimulation of synaptosomes by veratridine or A23187 for 5 min results in disassembly of the microtubules. The role of microtubule disassembly in neurotransmitter release was investigated using the microtubule-stabilizing drug ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91192-7
更新日期：1983-11-28 00:00:00
abstract：:A normobar hypoxia (9% oxygen) of 8 h reduces the neurotoxicity of a subcutaneous injection of 10 mg/kg kainic acid given one week later. Both seizures and degenerative changes, including cell death of hippocampal and cortical neurons are markedly decreased by hypoxia. It is also shown that hypoxia also markedly reduc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91693-4
更新日期：1994-05-02 00:00:00
abstract：:The purpose of this study was to identify the functional fields activated in relation to the self-paced proximal and distal arm movements. The regional cerebral blood flow (rCBF) was measured with positron emission tomography (PET) and 15O-labelled H2O (H2(15)O) in eight healthy subjects. All subjects performed the fo...
journal_title：Brain research
pub_type： 临床试验,杂志文章
doi：10.1016/0006-8993(96)00032-7
更新日期：1996-04-15 00:00:00
abstract：:Elevated levels of lactic acid can be deleterious to CNS tissue. Lactic acid is known to cause astroglial swelling and since glial swelling has been shown to inhibit L-glutamate (L-Glu) uptake, we examined whether one of the actions of lactic acid is to inhibit L-Glu uptake. Astrocyte cultures treated with lactic acid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(96)01331-5
更新日期：1997-03-07 00:00:00
abstract：:Fos-like immunoreactivity (FLI) can indicate the location of neurons activated following expression of conditioned taste aversion (CTA). After one conditioning trial FLI has been identified in the intermediate nucleus of the solitary tract (iNTS) with little expression in other brain regions. The present study assesse...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)03032-8
更新日期：2000-12-29 00:00:00
abstract：:To assess the role of androgen-dependent brain vasopressinergic transmission in the modulation of social recognition, castrated male rats which are deficient in vasopressin were implanted intracerebroventricularly with an Accurel collodion mini-device containing 10 micrograms vasopressin (AVP) whereas intact male rats...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90480-w
更新日期：1992-02-14 00:00:00
abstract：:Alpha-bungarotoxin binding sites in chick retina from 12 days in ovo to hatching, were visualized at the ultrastructural level by use of a 1:1 conjugate of horseradish peroxidase with alpha-bungarotoxin. At all stages binding sites in the inner plexiform layer were localized in synapses, predominantly on or near the p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90774-x
更新日期：1980-11-10 00:00:00
abstract：:The status of blood-brain barrier (BBB) permeability was investigated in the twitcher, an authentic murine model of globoid cell leukodystrophy (GLD, Krabbe disease) and cuprizone-intoxicated mice. Although extensive demyelination was noted in the CNS of both mice and additionally, macrophage infiltration was pronounc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90499-9
更新日期：1987-11-03 00:00:00
abstract：:The role of the central noradrenergic system in the supraoptic neuroendocrine regulation was investigated using slices of the guinea pig hypothalamus. Noradrenaline produced a complex membrane effect comprising two distinct depolarizations: one, associated with a moderate increase in input resistance and resulting in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91553-2
更新日期：1986-10-15 00:00:00
abstract：:Intracellular recordings were performed in vitro from both pars compacta and pars reticulata neurones of the substantia nigra, and their postsynaptic responses to electrical stimulation within the nucleus were analysed. Intracellular staining by biocytin was used to reveal the morphology and location of the recorded n...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91292-0
更新日期：1994-10-17 00:00:00
abstract：:We previously reported that the level of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) alpha and beta proteins increases with postnatal age. In the present study, we investigated the developmental changes in whole protein substrates of CaM kinase II as compared with those of cAMP-dependent protein kinase...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90861-3
更新日期：1994-10-03 00:00:00
abstract：:Reward anticipation is associated with activity in the dopaminergic midbrain as well as the ventral striatum, amygdala, and medial prefrontal cortex. Dopaminergic neuromodulation declines with age, suggesting that incentive processing should also undergo age-related change. However, the literature is mixed, perhaps re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.09.063
更新日期：2015-07-01 00:00:00
abstract：:Activation of kappa-opioid receptors (KOR) in the medial prefrontal cortex (mPFC) modulates excitatory transmission, which may involve interactions with N-methyl-D-aspartate (NMDA) glutamate receptors. We investigated possible anatomical correlates of this modulation by using dual labeling electron microscopy to exami...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02894-9
更新日期：2002-08-16 00:00:00
abstract：:In rats under urethane or pentobarbitone anesthesia, Ca2+ -sensitive microelectrodes were inserted into CA3 and CA1 hippocampal cells. In 23 neurons with a mean resting membrane potential (Vm) of -56.9 mV, the Ca potential (VCa) fell below Vm by an average of -22.1 mV (S.D. +/- 19.1 mV), indicating a mean intracellula...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90388-4
更新日期：1986-05-21 00:00:00
abstract：:In spite of the numerous reports on the optic fiber distribution in the optic nerve and tract of vertebrates, there have been few studies of the visual pathway in reptiles. The arrangement of fibers in the optic nerve and tract of the turtle Mauremys leprosa was studied by placing a small granule of carbocyanine dye (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.10.016
更新日期：2007-12-01 00:00:00
abstract：:Effects of alpha-adrenoceptor agents on electrophysiologically and immunohistochemically identified supraoptic nucleus (SON) vasopressin (VP) units were investigated by intracarotid infusion. Clonidine, an alpha 2-adrenoceptor agonist always excited SON units and alpha 2-adrenoceptor antagonists consistently inhibited...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91526-x
更新日期：1986-12-10 00:00:00
abstract：:The distribution of bFGF in mouse and rat retinas was established using an antibody generated against a rat specific bFGF sequence. The patterns established with this antibody were distinctly different in comparison to the immunostaining patterns using antibodies prepared against similar regions of bovine and human bF...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91236-8
更新日期：1992-07-10 00:00:00
abstract：:Fifteen adult male Sprague-Dawley rats were implanted with a set of electrodes for standard sleep recordings. A stainless steel cannula was also implanted into the lateral ventricle of these rats. Fifteen additional rats were implanted with a cannula alone. Rats with electrodes were habituated for 3 days or more to th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90888-5
更新日期：1994-10-03 00:00:00
