Abstract:
:Polyethylenimine (PEI) is widely used for non-viral transfection in vitro and in vivo. Hepatectomy is an interesting and considerable factor modifying PEI-mediated gene expression. We investigated the gene expression in mice over time following partial hepatectomy after an intravenous injection of PEI/plasmid DNA (pDNA) complex. pDNA encoding firefly luciferase was used as the model reporter gene. The hepatectomized liver was rapidly regenerated until 72 h. After 168 h, the liver weight of hepatectomized mice was similar to that of control mice. Slight liver function impairment was only observed at 1-24 h after hepatectomy in alanine aminotransferase and aspartate aminotransferase levels. Luciferase activity in the liver of partial hepatectomized mice at 48 h after partial hepatectomy increased by 70 times compared with that of control mice; however, luciferase activities did not significantly differ between hepatectomized mice and control mice in the spleen, lung, kidney, and heart. Among the lobes, luciferase activity by gram of tissue was not significantly different, indicating that gene expression enhancement by partial hepatectomy occurred equally throughout the liver. In conclusion, our findings demonstrate that liver resection is an influencing factor on PEI-mediated gene delivery in mice. These results indicate the necessity of considering cell division in PEI-mediated pDNA delivery.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Tada Y,Kitahara T,Yoshioka T,Nakamura T,Ichikawa N,Nakashima M,Nishida K,Nakamura J,Sasaki Hdoi
10.1248/bpb.29.1712subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1712-6issue
8eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/29.1712journal_volume
29pub_type
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journal_title:Biological & pharmaceutical bulletin
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