Abstract:
:Sjögren's syndrome (SS) is an autoimmune rheumatic disease that targets salivary and lachrymal glands, characterized by a high concentration of serum autoantibodies directed against nuclear and cytoplasmic antigens. It is known that autoantibodies can enter viable cells and this phenomenon has functional consequences including activation of apoptotic process. The objective of this work was to explore whether autoantibodies contained in IgG purified from Sjögren sera trigger apoptotic process in an experimental model represented by the human salivary gland cell line A-253. To define if the intrinsic or extrinsic pathways are activated, we examined which caspases are critical for inducing cell death. The results have demonstrated that morphological changes and DNA laddering, consistent with apoptotic cell death, occurred in A-253 cells treated with IgG from Sjögren sera. Sjögren IgG induced cleavage and activation of the effector caspase-3 and degradation of the caspase-3 substrate poly(ADP-ribose)polymerase. Both the intrinsic and extrinsic apoptotic pathways were activated, since both caspase-8 and caspase-9 cleavages occurred. In conclusion, autoantibodies contained in IgG purified from Sjögren sera mediate apoptosis of the A-253 cell line in a caspase-dependent manner.
journal_name
J Autoimmunjournal_title
Journal of autoimmunityauthors
Sisto M,Lisi S,Castellana D,Scagliusi P,D'Amore M,Caprio S,Scagliusi A,Acquafredda A,Panaro MA,Mitolo Vdoi
10.1016/j.jaut.2006.05.001subject
Has Abstractpub_date
2006-08-01 00:00:00pages
38-49issue
1eissn
0896-8411issn
1095-9157pii
S0896-8411(06)00035-7journal_volume
27pub_type
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