Abstract:
:Platelet-surface contact is the first step in thrombus formation. Platelet spreading makes this initial contact irreversible. On the other hand plasma lipids and fibrinogen have been described to activate platelets or promote adhesion. We therefore investigated whether platelet spreading under stagnation-point flow conditions correlated with plasma concentrations of cardiovascular risk factors such as fibrinogen and high density lipoprotein (HDL)-cholesterol. Platelet rich plasma (PRP) from patients with peripheral arterial occlusive disease and healthy controls was examined by means of the Stagnation-Point Flow Adhesio- Aggregometer (SPAA). The SPAA comprises a microscopic setup with a flow chamber that permits direct observation and quantitation of platelet deposition onto standardized surfaces. After the flow experiments the deposited platelets were analyzed morphometrically for the degree of spreading expressed as inverse circularity (1/C). 1/C was correlated over 2 X 2 tables of fibrinogen combined with plasma levels of HDL-cholesterol, each of which was divided into a low and high value group. The patient and control group differed significantly with regard to 1/C, i.e. patient platelets demonstrated more adhesive platelets with a more extensive degree of spreading. 1/C was inversely correlated with HDL-cholesterol and showed significant differences between the patient and the control group. Increased 1/C values were found when associated with high fibrinogen levels and simultaneously with low HDL-cholesterol concentrations. Platelet spreading shows a correlation with increased levels of independent plasmatic risk factors for thrombosis in PAOD patients. Obtained during stagnation-point flow, spreading seems to be a morphological marker for platelet hyperreactivity.
journal_name
Plateletsjournal_title
Plateletsauthors
Reininger AJ,Reininger CB,Agneskirchner J,Scherb H,Spannagl M,Wurzinger LJdoi
10.1080/09537109876771subject
Has Abstractpub_date
1998-01-01 00:00:00pages
241-4issue
3-4eissn
0953-7104issn
1369-1635pii
MHNVM7JCPPQL80VEjournal_volume
9pub_type
杂志文章相关文献
PLATELETS文献大全abstract::The action of inorganic forms of selenium (Se) on blood platelet aggregation, secretion and the arachidonate pathway in vitro was studied. It was shown that sodium selenite, after 30 min preincubation, inhibited platelet aggregation induced by 0.1 U/ml of thrombin and by 10 microM ADP (about 30% inhibition of aggregat...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109976275
更新日期:1999-01-01 00:00:00
abstract::Interaction of von Willebrand factor (VWF) with circulating platelets promotes hemostasis when a blood vessel is injured. The A1 domain of VWF is responsible for the initial interaction with platelets and is well conserved among species. Knowledge of the cDNA and genomic DNA sequences for human VWF allowed us to predi...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100600938816
更新日期:2007-05-01 00:00:00
abstract::Flow cytometric analysis of human platelets from healthy volunteers by high-resolution immunophenotyping accomplished with enzymatic amplification staining revealed the expression of CD34. Assessment of the platelets with five different monoclonal antibodies to the three different classes of CD34 epitopes suggested th...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/0953710031000080577
更新日期:2003-03-01 00:00:00
abstract::It has recently been demonstrated that both initiators and inhibitors (viz iloprost) of aggregation stimulate the uptake of [(45)Ca(2+)] by human platelets. Since it was postulated that this calcium uptake reflects changes associated with signal transduction, we investigated the role of cAMP-dependent protein kinases ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109309013234
更新日期:1993-01-01 00:00:00
abstract::In recent years the involvement of platelets dysfunction in atherogenesis and in the clinical complications from atherosclerosis has become more recognised. Systemic platelet-related thrombogenic factors have been shown to be involved in the initiation and progression of atherogenesis and plaque growth. Over the last ...
journal_title:Platelets
pub_type: 杂志文章,评审
doi:10.1080/0953770021000007221
更新日期:2002-08-01 00:00:00
abstract::The aim of this study was to determine the contribution of increased PGD(2) formation to the anti-aggregatory action of a thromboxane A(2) (TXA(2)) synthase inhibitor and TP receptor antagonist (ZD1542) against collagen-induced human platelet aggregation in citrated whole blood using the prostaglandin (PG) DP receptor...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109409005529
更新日期:1994-01-01 00:00:00
abstract::Disseminated intravascular coagulation (DIC) has a well-examined pathophysiology, yet some essential elements remain undetermined. During DIC, platelets play an important role in the development of micro thrombosis, but changes in platelet function parameters and their association with development of DIC have not been...
journal_title:Platelets
pub_type: 杂志文章,meta分析,评审
doi:10.1080/09537104.2018.1442567
更新日期:2018-05-01 00:00:00
abstract::Strenuous physical exercise leads to platelet activation that is normally counterbalanced by the production of endothelium-derived anti-platelet mediators, including prostacyclin (PGI2) and nitric oxide (NO). However, in the case of endothelial dysfunction, e.g. in atherosclerosis, there exists an increased risk for i...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1254764
更新日期:2017-11-01 00:00:00
abstract::Platelet aggregation in response to ADP, adrenaline, collagen, arachidonic acid and ristocetin was measured in a large population of healthy Saudi males (n = 393) and females (n = 221) whose age ranged from 11-60 years. The number of observations ranged from 130 for one of the doses of ristocetin (1.0 g/l) to 554 for ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109009005478
更新日期:1990-01-01 00:00:00
abstract::We previously reported that thrombin-induced Ca(2+) mobilization was enhanced in aged rat platelets. Since Ca(2+) mobilization in platelets is believed to be closely associated with platelet activation, we examined Ca(2+) mobilization and the aggregatory response to ADP and thrombin in young (3 months) and aged (24 mo...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509078477
更新日期:1995-01-01 00:00:00
abstract::Platelets contribute to blood coagulation at sites of vascular injury and to the recruitment of leukocytes at sites of inflammation. Under pathological conditions, platelets are involved in numerous diseases and clinical complications, such as deep venous thrombosis, embolism and atherosclerosis. But so far, little is...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537100903215417
更新日期:2009-11-01 00:00:00
abstract::Bioactive substances found in numerous foods can be successfully and safely used to modify various cellular functions and affect the oxidative stress. Aronia melanocarpa fruits (Rosaceae) are one of the richest plant sources of phenolic substances shown to have anti-inflammatory, antitumor, antioxidative and antiplate...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537101003612821
更新日期:2010-01-01 00:00:00
abstract::Platelets play a key role in thrombotic vascular occlusion at the side of an atherosclerotic plaque. Malondialdehyde (MDA) derivatives are components of oxidized LDL. Recent studies suggest enhanced adhesion of platelets on oxidized LDL, but evidence of MDA-modified LDL contribution to enhanced platelet adhesion is mi...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100500261590
更新日期:2006-03-01 00:00:00
abstract::Human melanoma cell Line, HMV-I, has been shown to induce platelet aggregation without thrombin generation. The platelet-aggregating activity of HMV-I cells increased with increasing culture periods of the cells. However, data indicate that the platelet-aggregating activity of HMV-I cells is dependent upon the cell de...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109509078456
更新日期:1995-01-01 00:00:00
abstract::Previous studies have reported increased platelet activation and aggregation in patients with obstructive sleep apnea (OSA). Continuous positive airway pressure (CPAP) treatment has been shown to decrease platelet activation. We aimed to study the effects of nasal CPAP therapy has on MPV values in patients with severe...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2011.578182
更新日期:2011-01-01 00:00:00
abstract::Recent studies suggest that anti-platelet agents are not equally effective in all individuals. We have developed a new method to evaluate the effect of anti-platelet drugs using the cone and plate(let) analyzer (CPA) test. The method is based on the ability of activators to reduce platelet adhesion under flow conditio...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100400028800
更新日期:2005-08-01 00:00:00
abstract::Thrombocytopenia is common in patients with lung diseases. Lung injury is associated with deranged platelet homeostasis. The potential mechanisms underlying this association were investigated in this study. The number and morphology of circulating megakaryocytes (MK) were investigated in vivo during cardiopulmonary by...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100600745120
更新日期:2006-08-01 00:00:00
abstract::Impaired glucose tolerance (IGT), a prediabetic state, is associated with an increased risk of cardiovascular disease. Mean platelet volume (MPV), a determinant of platelet activation, is a newly emerging risk factor for atherothrombosis. This study was designed to answer the following questions: (i) Do MPV levels cha...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100701609019
更新日期:2007-12-01 00:00:00
abstract::A controversial discussion as to whether human platelets are capable of regulated protein synthesis has been ongoing for over half a century. A previous study has suggested that human platelets synthesize large amounts of interleukin 1beta (IL-1beta) in response to external cues and in a physiologically significant ma...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100600800792
更新日期:2007-03-01 00:00:00
abstract::This study investigated the mechanism by which treatment of the CHRF 288-11 megakaryoblastic cell line with phorbol myristate acetate caused a transient increase in adhesion. The adhesion to tissue culture plastic occurred within 4 h and could be reversed by treatment with RGDS-peptide suggesting the involvement of on...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537109976176
更新日期:1999-01-01 00:00:00
abstract::Platelets are anucleated cells that upon activation by agonists or during storage may develop apoptotic events. The role of peroxynitrite and its reactive intermediates in apoptotic process in blood platelets is unknown. In order to study the appearance of biomarkers of apoptosis in platelets after treatment with pero...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100802406646
更新日期:2008-12-01 00:00:00
abstract::Platelet storage is associated with deleterious changes leading to the loss of platelet reactivity and response. During storage, platelets experience increased expression and shedding of P-selectin and CD40L as specific markers of platelet activation, whereas GPIbα decreases due to ectodomain shedding. As an important...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2016.1235692
更新日期:2017-07-01 00:00:00
abstract::Bernard-Soulier Syndrome (BSS) is an inherited recessive bleeding disorder. In some instances, diagnosis might be restricted to routine blood exams, including bleeding time, prothrombin time (PT), and partial thromboplastin time (APTT). Exams such as platelet aggregation, and testing for expression of ristocetin cofac...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100902893784
更新日期:2009-06-01 00:00:00
abstract::In humans and other mammals, Tityus discrepans (Td) scorpion envenomation produces a variety of systemic effects including respiratory distress, a generalized inflammatory reaction, modulation of blood pressure, fibrin formation, and platelet activation. For many of these effects, the venom components and underlying m...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2010.544343
更新日期:2011-01-01 00:00:00
abstract::Patients with thrombocytopenia or platelet disorders are at risk of severe bleeding. We report the development and validation of flow cytometry assays to diagnose platelet disorders and to assess platelet function independently of platelet count. The assays were developed to measure glycoprotein levels (panel 1) and p...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537104.2015.1032919
更新日期:2016-01-01 00:00:00
abstract::SUMMARY. Blood platelets exposed to hypotonic medium are known to undergo a regulatory volume decrease (RVD), mediated by increased conductive permeability of K(+) and Cl(-). It is presently shown that RVD in platelets is controlled by a lipoxygenase product, apparently by a selective regulation of K(+) permeability. ...
journal_title:Platelets
pub_type: 杂志文章
doi:10.3109/09537109109005512
更新日期:1991-01-01 00:00:00
abstract::Thrombocytopenia is common in the neonatal intensive care unit. Transfusion of platelets is often required. The purpose of our study was to determine changes in thrombopoietin (Tpo) following transfusion of platelets in preterm neonates. Preterm neonates undergoing platelet transfusion were randomized to receive a tra...
journal_title:Platelets
pub_type: 杂志文章,随机对照试验
doi:10.1080/09537100802220476
更新日期:2008-09-01 00:00:00
abstract::Catastrophic antiphospholipid syndrome (CAPS) involves sudden multiorgan dysfunction from thrombosis due to antibodies that cause platelet activation and endothelial dysfunction. Treatment variably combines anticoagulation, corticosteroid use, therapeutic plasma exchange (TPE), and high-dose intravenous immunoglobulin...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537104.2020.1802414
更新日期:2020-08-07 00:00:00
abstract::FVIII is an important cofactor in the tenase coagulation factor complex, lack of FVIII causes severe bleeding, whereas high FVIII levels seem to be associated with venous and arterial thromboembolism. Resting platelets do not bind FVIII, but activated platelets bind unactivated FVIII if vWF is not present. We investig...
journal_title:Platelets
pub_type: 杂志文章
doi:10.1080/09537100600747035
更新日期:2006-11-01 00:00:00
abstract::Activated platelets serve as a catalyst for thrombin generation and a source of vasoactive and mitogenic factors affecting vascular remodeling. Oral menopausal hormone treatments (MHT) may carry greater thrombotic risk than transdermal products. This study compared effects of oral and transdermal MHT on platelet chara...
journal_title:Platelets
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3109/09537104.2015.1023273
更新日期:2016-01-01 00:00:00