Silencing of Peroxiredoxin 2 and aberrant methylation of 33 CpG islands in putative promoter regions in human malignant melanomas.

Abstract:

:Aberrant methylation of promoter CpG islands (CGI) is involved in silencing of tumor suppressor genes and is also a potential cancer biomarker. Here, to identify CGIs aberrantly methylated in human melanomas, we did a genome-wide search using methylation-sensitive representational difference analysis. CGIs in putative promoter regions of 34 genes (ABHD9, BARHL1, CLIC5, CNNM1, COL2A1, CPT1C, DDIT4L, DERL3, DHRS3, DPYS, EFEMP2, FAM62C, FAM78A, FLJ33790, GBX2, GPR10, GPRASP1, HOXA9, HOXD11, HOXD12, HOXD13, p14ARF, PAX6, PRDX2, PTPRG, RASD1, RAX, REC8L1, SLC27A3, TGFB2, TLX2, TMEM22, TMEM30B, and UNC5C) were found to be methylated in at least 1 of 13 melanoma cell lines but not in two cultured normal melanocytes. Among these genes, Peroxiredoxin 2 (PRDX2) was expressed in normal melanocytes, and its expression was lost in melanomas with methylation. The loss of expression was restored by treatment of melanomas with a demethylating agent 5-aza-2'-deoxycytidine. In surgical melanoma specimens, methylation of PRDX2 was detected in 3 of 36 (8%). Furthermore, immunohistochemical analysis of PRDX2 showed that disappearance of immunoreactivity tends to associate with its methylation. PRDX2 was recently reported to be a negative regulator of platelet-derived growth factor signaling, and its silencing was suggested to be involved in melanomas. On the other hand, 12 CGIs were methylated in >or=9 of the 13 melanoma cell lines and are considered as candidate melanoma biomarkers.

journal_name

Cancer Res

journal_title

Cancer research

authors

Furuta J,Nobeyama Y,Umebayashi Y,Otsuka F,Kikuchi K,Ushijima T

doi

10.1158/0008-5472.CAN-06-0157

subject

Has Abstract

pub_date

2006-06-15 00:00:00

pages

6080-6

issue

12

eissn

0008-5472

issn

1538-7445

pii

66/12/6080

journal_volume

66

pub_type

杂志文章
  • Site-dependent differences in response of the UV-2237 murine fibrosarcoma to systemic therapy with adriamycin.

    abstract::Murine fibrosarcoma UV-2237MM cells were implanted into different organs of syngeneic C3H/HeN mice. The resultant tumors were treated by i.v. administration of Adriamycin (ADR). Despite the high sensitivity of the fibrosarcoma cells to ADR in vitro, the established tumors growing in vivo exhibited marked differences i...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Staroselsky AN,Fan D,O'Brian CA,Bucana CD,Gupta KP,Fidler IJ

    更新日期:1990-12-15 00:00:00

  • Periostin, a cell adhesion molecule, facilitates invasion in the tumor microenvironment and annotates a novel tumor-invasive signature in esophageal cancer.

    abstract::Human squamous cell cancers are the most common epithelially derived malignancies. One example is esophageal squamous cell carcinoma (ESCC), which is associated with a high mortality rate that is related to a propensity for invasion and metastasis. Here, we report that periostin, a highly expressed cell adhesion molec...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-10-0704

    authors: Michaylira CZ,Wong GS,Miller CG,Gutierrez CM,Nakagawa H,Hammond R,Klein-Szanto AJ,Lee JS,Kim SB,Herlyn M,Diehl JA,Gimotty P,Rustgi AK

    更新日期:2010-07-01 00:00:00

  • CYP17 and breast cancer risk: the polymorphism in the 5' flanking area of the gene does not influence binding to Sp-1.

    abstract::The ability of a motif of the CYP17 5' untranslated region, created by a polymorphic T to C substitution, to bind to the human transcription factor Sp-1 was investigated. No binding of any of the polymorphic alleles was observed in electromobility shift assay. No other sequence within +1 to +100 of each of the CYP17 a...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Nedelcheva Kristensen V,Haraldsen EK,Anderson KB,Lønning PE,Erikstein B,Kåresen R,Gabrielsen OS,Børresen-Dale AL

    更新日期:1999-06-15 00:00:00

  • Metabolic activation of temozolomide measured in vivo using positron emission tomography.

    abstract::The purpose of this research was to quantitate and confirm the mechanism of in vivo metabolic activation of temozolomide. The secondary aims were to evaluate the tumor, normal tissue, and plasma pharmacokinetics of temozolomide in vivo, and to determine whether such pharmacokinetics resulted in tumor targeting. [(11)C...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Saleem A,Brown GD,Brady F,Aboagye EO,Osman S,Luthra SK,Ranicar AS,Brock CS,Stevens MF,Newlands E,Jones T,Price P

    更新日期:2003-05-15 00:00:00

  • Partial synchronization of L1210 cells by 5-fluorouracil and its use in drug combinations.

    abstract::When L1210 cells growing logarithmically were exposed for 8 hr to a nonlethal dose of 5-fluorouracil (FU) (0.25 microgram/ml), the percentage of cells in S phase increased from 74.9% in the asynchronous culture to 93% in the FU-treated culture. This resulted in increased cell-kill by S-phase-specific inhibitors [1-bet...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bhuyan BK,Blowers CL,Neil GL,Bono VH,Day KJ

    更新日期:1977-09-01 00:00:00

  • Gamma-irradiation-induced DNA damage checkpoint activation involves feedback regulation between extracellular signal-regulated kinase 1/2 and BRCA1.

    abstract::Previous studies from our laboratory have shown that the activation of G(2)-M checkpoint after exposure of MCF-7 breast cancer cells to gamma-irradiation (IR) is dependent on the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) signaling. Studies presented in this report indicate that IR exposure of MC...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-07-5818

    authors: Yan Y,Black CP,Cao PT,Haferbier JL,Kolb RH,Spieker RS,Ristow AM,Cowan KH

    更新日期:2008-07-01 00:00:00

  • Matrix metalloproteinase 2 in tumor cell-induced platelet aggregation: regulation by nitric oxide.

    abstract::A correlation exists between the ability of tumor cells to aggregate platelets and their tendency to metastasize. Tumor cell-induced platelet aggregation (TCIPA) facilitates the embolization of the vasculature with tumor cells and the formation of metastatic foci. It is well documented that matrix metalloproteinases (...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Jurasz P,Sawicki G,Duszyk M,Sawicka J,Miranda C,Mayers I,Radomski MW

    更新日期:2001-01-01 00:00:00

  • Dissecting karyotypic patterns in colorectal tumors: two distinct but overlapping pathways in the adenoma-carcinoma transition.

    abstract::More than 500 colorectal tumors with clonal chromosomal abnormalities have been reported. Although the pattern of aberrations is nonrandom, no specific primary or secondary karyotypic abnormality has been identified. Also, the chronological order in which the aberrations appear during disease progression is not well k...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Höglund M,Gisselsson D,Hansen GB,Säll T,Mitelman F,Nilbert M

    更新日期:2002-10-15 00:00:00

  • Parkin pathway activation mitigates glioma cell proliferation and predicts patient survival.

    abstract::Mutations in the parkin gene, which encodes a ubiquitin ligase, are a major genetic cause of parkinsonism. Interestingly, parkin also plays a role in cancer as a putative tumor suppressor, and the gene is frequently targeted by deletion and inactivation in human malignant tumors. Here, we investigated a potential tumo...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-11-3060

    authors: Yeo CW,Ng FS,Chai C,Tan JM,Koh GR,Chong YK,Koh LW,Foong CS,Sandanaraj E,Holbrook JD,Ang BT,Takahashi R,Tang C,Lim KL

    更新日期:2012-05-15 00:00:00

  • Biological purging of breast cancer cells using an attenuated replication-competent herpes simplex virus in human hematopoietic stem cell transplantation.

    abstract::Autologous hematopoietic stem cell transplantation after myelosuppressive chemotherapy is used for the treatment of high-risk breast cancer and other solid tumors. However, contamination of the autologous graft with tumor cells may adversely affect outcomes. Human hematopoietic bone marrow cells are resistant to herpe...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Wu A,Mazumder A,Martuza RL,Liu X,Thein M,Meehan KR,Rabkin SD

    更新日期:2001-04-01 00:00:00

  • Elements related to heterogeneity of antibody-dependent cell cytotoxicity in patients under trastuzumab therapy for primary operable breast cancer overexpressing Her2.

    abstract::Preliminary results from a pilot trial on trastuzumab's mechanism of action against operable breast tumors overexpressing Her2 suggested a role for antibody-dependent cell cytotoxicity (ADCC). To examine factors affecting ADCC intensity and variability, we extended this study to the phenotypic and functional analysis ...

    journal_title:Cancer research

    pub_type: 临床试验,杂志文章

    doi:10.1158/0008-5472.CAN-07-2068

    authors: Varchetta S,Gibelli N,Oliviero B,Nardini E,Gennari R,Gatti G,Silva LS,Villani L,Tagliabue E,Ménard S,Costa A,Fagnoni FF

    更新日期:2007-12-15 00:00:00

  • Targeting PIM kinases impairs survival of hematopoietic cells transformed by kinase inhibitor-sensitive and kinase inhibitor-resistant forms of Fms-like tyrosine kinase 3 and BCR/ABL.

    abstract::Previous studies have shown that activation of the signal transducer and activator of transcription 5 (STAT5) plays an essential role in leukemogenesis mediated through constitutive activated protein tyrosine kinases (PTK). Because PIM-1 is a STAT5 target gene, we analyzed the role of the family of PIM serine/threonin...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-2309

    authors: Adam M,Pogacic V,Bendit M,Chappuis R,Nawijn MC,Duyster J,Fox CJ,Thompson CB,Cools J,Schwaller J

    更新日期:2006-04-01 00:00:00

  • Mapping of ER gene CpG island methylation-specific polymerase chain reaction.

    abstract::Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) alpha-negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of the entire ER CpG isl...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Lapidus RG,Nass SJ,Butash KA,Parl FF,Weitzman SA,Graff JG,Herman JG,Davidson NE

    更新日期:1998-06-15 00:00:00

  • Changes in thermosensitivity of mouse mammary carcinoma following hyperthermia in vivo.

    abstract::The time course of change in thermosensitivity of SCK tumor cells in vivo was investigated following preheating at 43.5 degrees for 30 min. At varying times after the preheating with water bath, tumors were subjected to graded doses of second heating at 43.5 degrees in vivo, and cell survival was determined by using i...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Rhee JG,Song CW,Levitt SH

    更新日期:1982-11-01 00:00:00

  • Importance of tumor-specific cytotoxic CD8+ T-cells in eradication of a large subcutaneous MOPC-315 tumor following low-dose melphalan therapy.

    abstract::We have previously demonstrated that depletion of CD8+ T-cells by the use of a monoclonal anti-Lyt-2.2 antibody abolishes the curative effectiveness of low-dose melphalan (L-phenylalanine mustard; L-PAM) therapy for BALB/c mice bearing a large (greater than or equal to 20 mm) s.c. MOPC-315 tumor and extensive metastas...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Takesue BY,Pyle JM,Mokyr MB

    更新日期:1990-12-01 00:00:00

  • Increased mitotic phosphorylation of histone H3 attributable to AIM-1/Aurora-B overexpression contributes to chromosome number instability.

    abstract::Phosphorylation of histone H3 at Ser-10 is required for maintenance of properchromosome dynamics during mitosis. AIM-1, a mammalian Ipl1/aurora kinase involved in H3 phosphorylation, is transcriptionally overexpressed in many tumor cell lines. Increased expression of the AIM-1 gene has been observed in human colorecta...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Ota T,Suto S,Katayama H,Han ZB,Suzuki F,Maeda M,Tanino M,Terada Y,Tatsuka M

    更新日期:2002-09-15 00:00:00

  • A Novel MIF Signaling Pathway Drives the Malignant Character of Pancreatic Cancer by Targeting NR3C2.

    abstract::Pancreatic cancers with aberrant expression of macrophage migration inhibitory factor (MIF) are particularly aggressive. To identify key signaling pathways that drive disease aggressiveness in tumors with high MIF expression, we analyzed the expression of coding and noncoding genes in high and low MIF-expressing tumor...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-15-2841

    authors: Yang S,He P,Wang J,Schetter A,Tang W,Funamizu N,Yanaga K,Uwagawa T,Satoskar AR,Gaedcke J,Bernhardt M,Ghadimi BM,Gaida MM,Bergmann F,Werner J,Ried T,Hanna N,Alexander HR,Hussain SP

    更新日期:2016-07-01 00:00:00

  • alpha6beta1-Integrin, a major cell surface carrier of beta1-6-branched oligosaccharides, mediates migration of EJ-ras-transformed fibroblasts on laminin-1 independently of its glycosylation state.

    abstract::EJ-ras oncogene-induced malignant transformation is characterized by a series of changes in cell surface carbohydrates and cell-cell and cell-matrix interactions. Here, we show that EJ-ras-transformed NIH-3T3 fibroblasts acquired a migratory phenotype on laminin-1 surfaces. Such a phenotype was accompanied by overexpr...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Jasiulionis MG,Chammas R,Ventura AM,Travassos LR,Brentani RR

    更新日期:1996-04-01 00:00:00

  • CXCL10 promotes invasion-related properties in human colorectal carcinoma cells.

    abstract::CXCL10 was recently shown to exert antimalignancy functions by influencing the tumor microenvironment. Here, we have taken a different approach, investigating the effects of CXCL10 directly on tumor-promoting functions in colorectal carcinoma (CRC) cells. CXCL10 expression was detected in preferred metastatic sites of...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-3087

    authors: Zipin-Roitman A,Meshel T,Sagi-Assif O,Shalmon B,Avivi C,Pfeffer RM,Witz IP,Ben-Baruch A

    更新日期:2007-04-01 00:00:00

  • The human REC2/RAD51B gene acts as a DNA damage sensor by inducing G1 delay and hypersensitivity to ultraviolet irradiation.

    abstract::HsRec2/Rad51B is a 350-amino acid protein with a molecular mass of 38,300 Da that appears to be involved in cell cycle regulation and UV-induced apoptosis. The mouse and human genes were isolated based on their homology to a recombinational repair gene from Ustilago maydis and contain functional domains to hRAD51 and ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Havre PA,Rice MC,Noe M,Kmiec EB

    更新日期:1998-10-15 00:00:00

  • Heterogeneity of the surface material in isolated cells of transplantable hamster melanomas.

    abstract::The heterogeneity of the surface material released by trypsin in isolated cells of melanotic and amelanotic melanomas was studied by the method of separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The electrophoretic patterns of the surface material derived from two kinds of melanoma showed some...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kozłowska K,Zurawska-Czupa B

    更新日期:1983-03-01 00:00:00

  • Two distinct mechanisms of antitumor activity mediated by the combination of interleukin 2 and monoclonal antibodies.

    abstract::Previously we described a human B-lymphoma xenotransplantation model in which the immunotherapeutic activity of monoclonal antibodies (mAbs), directed against human B-cell antigens, can be studied. An anti-CD19 mAb of IgG2a subclass was therapeutically active by itself in this model, and its efficacy was increased by ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Vuist WM,van Buitenen F,Hekman A,Melief CJ

    更新日期:1990-09-15 00:00:00

  • Reactive Oxygen Species Drive Proliferation in Acute Myeloid Leukemia via the Glycolytic Regulator PFKFB3.

    abstract::Acute myeloid leukemia (AML) is a heterogeneous clonal disorder with a poor clinical outcome. Previously, we showed that overproduction of reactive oxygen species (ROS), arising from constitutive activation of NOX2 oxidase, occurs in >60% of patients with AML and that ROS production promotes proliferation of AML cells...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-19-1920

    authors: Robinson AJ,Hopkins GL,Rastogi N,Hodges M,Doyle M,Davies S,Hole PS,Omidvar N,Darley RL,Tonks A

    更新日期:2020-03-01 00:00:00

  • Genomic and expression analysis of the 12p11-p12 amplicon using EST arrays identifies two novel amplified and overexpressed genes.

    abstract::We performed parallel array comparative genomic hybridization and array expression analysis of the 12p11-p12 amplicon in human testicular seminomas and an ovarian carcinoma cell line using an expressed sequence tags (ESTs) array spotted with 8254 ESTs. The data were normalized using a robust statistical modeling and t...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Bourdon V,Naef F,Rao PH,Reuter V,Mok SC,Bosl GJ,Koul S,Murty VV,Kucherlapati RS,Chaganti RS

    更新日期:2002-11-01 00:00:00

  • Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy.

    abstract::We have defined the chromosomal location of the human MAGE (melanoma antigen) gene family by polymerase chain reaction amplification of several segments of the MAGE genes from somatic cell hybrids containing well-defined groups of human chromosomes and hybrids containing human derivative chromosomes. The data show tha...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Oaks MK,Hanson JP Jr,O'Malley DP

    更新日期:1994-04-01 00:00:00

  • Differential metabolic patterns of iodinated versus radiometal chelated anticarcinoma single-chain Fv molecules.

    abstract::Genetically engineered single-chain Fvs (sFv) are defined as recombinant proteins composed of a variable light chain amino acid sequence of an immunoglobulin tethered to a variable heavy chain sequence by a designed peptide. Previous studies using iodine-labeled sFv, derived from the anticarcinoma monoclonal antibody ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Schott ME,Milenic DE,Yokota T,Whitlow M,Wood JF,Fordyce WA,Cheng RC,Schlom J

    更新日期:1992-11-15 00:00:00

  • Prognostic index for clinical Stage I cutaneous malignant melanoma.

    abstract::Thirteen variables were evaluated for their significance in predicting the survival of 148 patients in a retrospective study with clinical Stage I cutaneous malignant melanoma. The variables studied were histological type, tumor thickness, level of invasion, mitotic activity, pigmentation type, existence of ulceration...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Hacene K,Le Doussal V,Brunet M,Lemoine F,Guerin P,Hebert H

    更新日期:1983-06-01 00:00:00

  • E-cadherin phosphorylation by protein kinase D1/protein kinase C{mu} is associated with altered cellular aggregation and motility in prostate cancer.

    abstract::The cadherin family of transmembrane glycoproteins plays a critical role in cell-to-cell adhesion and cadherin dysregulation is strongly associated with cancer metastasis and progression. In this study, we report a novel interaction between protein kinase D1 [PKD1; formerly known as protein kinase C mu (PKCmu)] and E-...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Jaggi M,Rao PS,Smith DJ,Wheelock MJ,Johnson KR,Hemstreet GP,Balaji KC

    更新日期:2005-01-15 00:00:00

  • ING1 represses transcription by direct DNA binding and through effects on p53.

    abstract::The ING family of proteins is involved in the regulation of diverse processes ranging from cell cycle and cellular senescence to apoptosis. These effects are most likely through activation of acetylation-dependent pathways that ultimately alter gene expression. Despite reports linking ING to p53 activation, the molecu...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kataoka H,Bonnefin P,Vieyra D,Feng X,Hara Y,Miura Y,Joh T,Nakabayashi H,Vaziri H,Harris CC,Riabowol K

    更新日期:2003-09-15 00:00:00

  • Increased level of bleomycin-induced chromosome breakage in ataxia telangiectasia skin fibroblasts.

    abstract::Ataxia telangiectasia (AT) is an autosomal recessive disorder in which increased level of chromosome breakage and specific sensitivity to radiation and carcinogens have been reported. The effect of the radiomimetic drug bleomycin on chromosome breakage has been tested in skin fibroblasts of three patients with AT, two...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Shaham M,Becker Y,Lerer I,Voss R

    更新日期:1983-09-01 00:00:00