Substrate specificity of Arabidopsis 3-ketoacyl-CoA synthases.


:The very long chain fatty acids (VLCFA) incorporated into plant lipids are derived from the iterative addition of C2 units provided by malonyl-CoA to an acyl-CoA by the 3-ketoacyl-CoA synthase (KCS) component of a fatty acid elongase (FAE) complex. Mining of the Arabidopsis genome sequence database revealed 20 genes with homology to seed-specific FAE1 KCS. Eight of the 20 putative KCSs were cloned, expressed in yeast, and isolated as (His)6 fusion proteins. Five of the eight (At1g71160, At1g19440, At1g07720, At5g04530, and At4g34250) had little or no activity with C16 to C20 substrates while three demonstrated activity with C16, C18, and C20 saturated acyl-CoA substrates. At1g01120 KCS (KCS1) and At2g26640 KCS had broad substrate specificities when assayed with saturated and mono-unsaturated C16 to C24 acyl-CoAs while At4g34510 KCS was specific for saturated fatty acyl-CoA substrates.


Blacklock BJ,Jaworski JG




Has Abstract


2006-07-28 00:00:00














  • The eta isoform of protein kinase C inhibits UV-induced activation of caspase-3 in normal human keratinocytes.

    abstract::Protein kinase C (PKC) fulfills a central role in the decision of cell fate in keratinocytes. Both PKC delta and PKC eta induce growth inhibition and differentiation of normal human keratinocytes (NHK). Here we show that PKC delta and PKC eta play opposite roles in UVB-induced apoptosis in NHK. PKC delta enhanced UVB-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Matsumura M,Tanaka N,Kuroki T,Ichihashi M,Ohba M

    更新日期:2003-03-28 00:00:00

  • Target disruption of ribosomal protein pNO40 accelerates aging and impairs osteogenic differentiation of mesenchymal stem cells.

    abstract::pNO40/PS1D, a novel nucleolar protein, has been characterized as a core protein of eukaryotic 60S ribosome and at least two splicing forms of pNO40 mRNAs with alternative starting sites have been identified. Through production of knockout (ko) mice with either exon 2 (△E2), exon 4 (△E4) or △E2+E4 targeted disruption w...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin YM,Wu CC,Chang YC,Wu CH,Ho HL,Hu JW,Chang RC,Wang CT,Ouyang P

    更新日期:2016-01-22 00:00:00

  • IKK regulates the deubiquitinase CYLD at the postsynaptic density.

    abstract::K63-linked polyubiquitination of proteins regulates their trafficking into specific cellular pathways such as endocytosis and autophagy. CYLD, a deubiquitinase specific for K63-linked polyubiquitins, is present in high quantities at the postsynaptic density (PSD). It was previously shown that, under excitatory conditi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Thein S,Pham A,Bayer KU,Tao-Cheng JH,Dosemeci A

    更新日期:2014-07-18 00:00:00

  • cDNA cloning and characterization of the human UDP glucuronosyltransferase, UGT1A3.

    abstract::The cDNA encoding the UDP glucuronosyltransferase, UGT1A3, has been cloned and expressed in cell culture. The deduced amino acid sequence of the cDNA is 90% similar in sequence to that of a previously characterized form, UGT1A4 and to that of a third form, UGT1A5 whose function in unknown. UGT1A3, when expressed in CO...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mojarrabi B,Butler R,Mackenzie PI

    更新日期:1996-08-23 00:00:00

  • Circular RNA hsa_circ_0008039 promotes breast cancer cell proliferation and migration by regulating miR-432-5p/E2F3 axis.

    abstract::As the development of sequencing technology, more and more circular RNAs (circRNAs) are identified in human cancer tissues. Increasing evidences imply circRNAs are important regulators in tumor progression. Nevertheless, how circRNAs participate in breast cancer development and progression is not well understood. In t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu Y,Lu C,Zhou Y,Zhang Z,Sun L

    更新日期:2018-07-20 00:00:00

  • Dystrophin-related protein in the fetal and denervated skeletal muscles of normal and mdx mice.

    abstract::The amino acid sequence of the polyclonal antibodies we developed against the carboxyl terminus of the dystrophin-related protein, the putative gene product of B3 cDNA, had no homologous sequence to the dystrophin molecule except for two amino acids located at its ends for immunization. By immunohistochemical examinat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takemitsu M,Ishiura S,Koga R,Kamakura K,Arahata K,Nonaka I,Sugita H

    更新日期:1991-11-14 00:00:00

  • argJ mutations are highly inducible by ethidium bromide in proB strains of Streptomyces lividans: implication of pathway interactions.

    abstract::Spontaneous Arg- mutants arose at high frequencies in Streptomyces lividans. Exposure to ethidium bromide increased the frequency of arg instability. In Pro+ strains the induced arg mutants were mainly argG, but in the proB mutants, a new mutation, argJ, prevailed which lacked ornithine acetyltransferase activity and ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chou CF,Chen CW

    更新日期:1992-12-15 00:00:00

  • Involvement of transcription repressor Snail in the regulation of human telomerase reverse transcriptase (hTERT) by transforming growth factor-β.

    abstract::Human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is the primary determinant for telomerase enzyme activity, which has been associated with cellular immortality. Expression of the hTERT gene is regulated by various extracellular (external) stimuli and is aberrantly up-regulated in more...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yoo YS,Park S,Gwak J,Ju BG,Oh S

    更新日期:2015-09-11 00:00:00

  • Effect of oxidation of the non-catalytic β-propeller domain on the substrate specificity of prolyl oligopeptidase from Pleurotus eryngii.

    abstract::Enzymes belonging to the S9 family of prolyl oligopeptidases are of interest because of their pharmacological importance and have a non-catalytic β-propeller domain. In this study, we found that the oxidation of Met203, which lies on surface of the β-propeller domain, leads to change in the substrate specificity of er...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tokai S,Bito T,Shimizu K,Arima J

    更新日期:2017-05-27 00:00:00

  • Thrombin regulates matrix metalloproteinase-9 expression in human monocytes.

    abstract::We investigated whether thrombin, the final activator of coagulation cascade, regulates expression of matrix metalloproteinases (MMP)-9 in human monocytes. We show that thrombin stimulation induced MMP-9 secretion of monocytes dose- and time-dependently as revealed by gelatin zymography. Real-time RT-PCR and Western b...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chang CJ,Hsu LA,Ko YH,Chen PL,Chuang YT,Lin CY,Liao CH,Pang JH

    更新日期:2009-07-24 00:00:00

  • Structural characterization of the human estrogen synthetase (aromatase) gene.

    abstract::The estrogen synthetase (aromatase, cytochrome P-450AROM) gene has been isolated from human genomic libraries and characterized. The restriction map of 43 positive clones obtained indicated that this enzyme is present as a single copy gene. The aromatase gene is unexpectedly large compared with other forms of the cyto...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Harada N,Yamada K,Saito K,Kibe N,Dohmae S,Takagi Y

    更新日期:1990-01-15 00:00:00

  • Molecular cloning and characterization of human FGF-20 on chromosome 8p21.3-p22.

    abstract::The fibroblast growth factors (FGFs) play important roles in morphogenesis, angiogenesis, tissue remodeling, and carcinogenesis. Human FGF-20 has been cloned and characterized in this study. FGF-20 encodes a 211-amino-acid polypeptide with the FGF-core domain. A strong hydrophobic region was found in the FGF-core doma...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kirikoshi H,Sagara N,Saitoh T,Tanaka K,Sekihara H,Shiokawa K,Katoh M

    更新日期:2000-08-02 00:00:00

  • Functional interaction between nonreceptor tyrosine kinase c-Abl and SR-Rich protein RBM39.

    abstract::RBM39, also known as splicing factor HCC1.4, acts as a transcriptional coactivator for the steroid nuclear receptors JUN/AP-1, ESR1/ER-α and ESR2/ER-β. RBM39 is involved in the regulation of the transcriptional responses of these steroid nuclear receptors and promotes transcriptional initiation. In this paper, we repo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mai S,Qu X,Li P,Ma Q,Liu X,Cao C

    更新日期:2016-04-22 00:00:00

  • Alpha 1-adrenergic and muscarinic-cholinergic stimulated inositol trisphosphate production may proceed through different post-receptor signal transduction pathways in parotid acini.

    abstract::Maximal stimulation of inositol 1,4,5 trisphosphate (IP3) production by epinephrine and carbachol in rat parotid cell aggregates is additive when the two agents are employed simultaneously. The additive response proceeds through both the alpha 1-adrenergic and muscarinic-cholinergic signal transduction pathways. It is...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kowatch MA,Roth GS

    更新日期:1991-05-15 00:00:00

  • Conformational dynamics of loops L11 and L12 of kinesin as revealed by spin-labeling EPR.

    abstract::The EPR spectra of the spin labels attached to loops L11 and L12 of kinesin were resolved into slow (rotational correlation time, tau=10-45 ns) and fast (tau=2 ns) components. The fraction of the slow component increased considerably when kinesin was complexed with a microtubule (MT). On MT binding and in the presence...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yamada MD,Maruta S,Yasuda S,Kondo K,Maeda H,Arata T

    更新日期:2007-12-21 00:00:00

  • Cell adhesion to ephrinb2 is induced by EphB4 independently of its kinase activity.

    abstract::Cell to cell interaction in bone marrow is crucial for differentiation of hematopoietic cells. We have shown that EphB4 receptor is expressed in erythroid progenitor and its activation accelerates erythroid differentiation. To elucidate the role of EphB4 activation in erythropoiesis, we analyzed effects of EphB4 on ce...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakamoto H,Zhang XQ,Suenobu S,Ohbo K,Ogawa M,Suda T

    更新日期:2004-08-27 00:00:00

  • Inhibition of periarterial nerve stimulation-induced vasodilation of the mesenteric arterial bed by CGRP (8-37) and CGRP receptor desensitization.

    abstract::We provide the first functional evidence that calcitonin gene-related peptide (8-37) induces a direct vasoconstriction and reversibly antagonizes vasodilation of the mesenteric arterial bed induced by calcitonin gene-related peptide (CGRP) suggesting that CGRP (8-37) is a competitive antagonist of vascular CGRP recept...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Han SP,Naes L,Westfall TC

    更新日期:1990-04-30 00:00:00

  • Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells.

    abstract::Hydrogen sulfide (H(2)S) is an important gasotransmitter that generated in mammalian cells from l-cysteine metabolism. Little is known about its protective role in oxidative stress. In the present study, we investigated whether H(2)S could affect homocysteine (HCY)-induced cytotoxicity and oxidative stress in vascular...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yan SK,Chang T,Wang H,Wu L,Wang R,Meng QH

    更新日期:2006-12-15 00:00:00

  • Differential effects of protein phosphatase 2A on distinguishable intracellular signals initiated by v-Src and mediated by HaRas.

    abstract::v-Src activates gene expression mediated by serum response elements (SREs) and TPA response elements (TREs). v-Src-induced SRE- and TRE-mediated gene expressions are both dependent upon HaRas. Protein phosphatase 2A (PP2A) is a serine/threonine phosphatase that has been implicated in v-Src-initiated signals. We report...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gupta R,Foster DA

    更新日期:1994-12-15 00:00:00

  • Pre-clinical evaluation of cinobufotalin as a potential anti-lung cancer agent.

    abstract::Lung cancer is a major cause of cancer-related mortality in the United States and around the world. Due to the pre-existing or acquired chemo-resistance, the current standard chemotherapy regimens only show moderate activity against lung cancer. In the current study, we explored the potential anti-lung cancer activity...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kai S,Lu JH,Hui PP,Zhao H

    更新日期:2014-09-26 00:00:00

  • Diethylnitrosamine and thioacetamide-induced hepatic damage and early carcinogenesis in rats: Role of Nrf2 activator dimethyl fumarate and NLRP3 inhibitor glibenclamide.

    abstract::Two-stage rat hepatocarcinogenesis model was used to induce early carcinogenesis in which thioacetamide (TAA) promotes diethylnitrosamine (DEN) initiated carcinogenesis. Dimethyl fumarate (DMF) used to treat multiple sclerosis, activates the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant responsive ele...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dwivedi DK,Jena GB

    更新日期:2020-02-05 00:00:00

  • CTRP3 plays an important role in the development of collagen-induced arthritis in mice.

    abstract::Rheumatoid arthritis (RA) is an autoimmune inflammatory disease exhibited most commonly in joints. We found that the expression of C1qtnf3, which encodes C1q/TNF-related protein 3 (CTRP3), was highly increased in two mouse RA models with different etiology. To elucidate the pathogenic roles of CTRP3 in the development...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Murayama MA,Kakuta S,Maruhashi T,Shimizu K,Seno A,Kubo S,Sato N,Saijo S,Hattori M,Iwakura Y

    更新日期:2014-01-03 00:00:00

  • Noninvasive, targeted gene therapy for acute spinal cord injury using LIFU-mediated BDNF-loaded cationic nanobubble destruction.

    abstract::Various gene delivery systems have been widely studied for the acute spinal cord injury (SCI) treatment. In the present study, a novel type of brain-derived neurotrophic factor (BDNF)-loaded cationic nanobubbles (CNBs) conjugated with MAP-2 antibody (mAbMAP-2/BDNF/CNBs) was prepared to provide low-intensity focused ul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Song Z,Ye Y,Zhang Z,Shen J,Hu Z,Wang Z,Zheng J

    更新日期:2018-02-12 00:00:00

  • KSHV RTA induces a transcriptional repressor, HEY1 that represses rta promoter.

    abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is a gamma-herpesvirus that is frequently associated with the formation of tumors like Kaposi's sarcoma and primary effusion lymphomas. KSHV RTA is required for lytic replication/reactivation in KSHV-infected cells. We developed an inducible RTA expression cell line to se...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yada K,Do E,Sakakibara S,Ohsaki E,Ito E,Watanabe S,Ueda K

    更新日期:2006-06-23 00:00:00

  • Maintained activity of glycogen synthase kinase-3beta despite of its phosphorylation at serine-9 in okadaic acid-induced neurodegenerative model.

    abstract::Glycogen synthase kinase-3beta (GSK3beta) is recognized as one of major kinases to phosphorylate tau in Alzheimer's disease (AD), thus lots of AD drug discoveries target GSK3beta. However, the inactive form of GSK3beta which is phosphorylated at serine-9 is increased in AD brains. This is also inconsistent with phosph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lim YW,Yoon SY,Choi JE,Kim SM,Lee HS,Choe H,Lee SC,Kim DH

    更新日期:2010-04-30 00:00:00

  • Inhibition of interferon-gamma-activated nuclear factor-kappa B by cyclosporin A: A possible mechanism for synergistic induction of apoptosis by interferon-gamma and cyclosporin A in gastric carcinoma cells.

    abstract::We previously reported synergistic induction of apoptosis by IFN-gamma plus either cyclosporin A (CsA) or tacrolimus (FK506) in gastric carcinoma cells. In this study, we aimed to elucidate the mechanism for this synergistic induction of apoptosis. IFN-gamma plus CsA synergistically induced caspase-3 mediated apoptosi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Beppu K,Morisaki T,Matsunaga H,Uchiyama A,Ihara E,Hirano K,Kanaide H,Tanaka M,Katano M

    更新日期:2003-06-13 00:00:00

  • Secretion of insulin-like growth factor II into milk.

    abstract::125I-labeled insulin-like growth factor II (IGF-II) was infused directly into the pudic artery supplying one gland of lactating goats (n = 4). Maximum specific activity for [125I]IGF-II transferred into milk from the infused gland was reached 60 min after that in plasma and was 2.5 fold greater than in milk from the n...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Prosser CG,Fleet IR

    更新日期:1992-03-31 00:00:00

  • An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin.

    abstract::Trichosanthin (TCS) is a type I ribosome-inactivating protein possessing multiple biological and pharmacological activities. One of its major actions is inhibition of human immunodeficiency virus (HIV) replication. The mechanism is still not clear. It is generally believed that this action is mediated via ribosome ina...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ouyang DY,Chan H,Wang YY,Huang H,Tam SC,Zheng YT

    更新日期:2006-01-06 00:00:00

  • Oxidized low density lipoprotein suppresses lipopolysaccharide-induced inflammatory responses in microglia: oxidative stress acts through control of inflammation.

    abstract::Low density lipoprotein (LDL) is readily oxidized under certain conditions, resulting in the formation of oxidized LDL (oxLDL). Despite numerous in vitro reports that reveal the pathogenic role of oxidative stress, anti-oxidative strategies have underperformed in the clinic. In this study, we examine the role of oxLDL...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim OS,Lee CS,Joe EH,Jou I

    更新日期:2006-03-31 00:00:00

  • X-ray structure of a protease-resistant mutant form of human galectin-9 having two carbohydrate recognition domains with a metal-binding site.

    abstract::Galectin-9 (G9) is a tandem-repeat type β-galactoside-specific animal lectin having N-terminal and C-terminal carbohydrate recognition domains (N-CRD and C-CRD, respectively) joined by a linker peptide that is involved in the immune system. G9 is divalent in glycan binding, and structural information about the spatial...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yoshida H,Nishi N,Wada K,Nakamura T,Hirashima M,Kuwabara N,Kato R,Kamitori S

    更新日期:2017-09-02 00:00:00