Reduction in the mutagenicity of synthetic dyes by successive treatment with activated sludge and the ligninolytic fungus, Irpex lacteus.


:Synthetic dyes are released in wastewater from textile manufacturing plants, and many of these dyes are genotoxic. In the present study, the mutagenicity of azo, anthraquinone, and triphenyl methane dyes was investigated before and after successive biodegradation with activated sludge and the ligninolytic fungus, Irpex lacteus. Two biodegradation systems were used to reduce the genotoxicity of dyes that were not efficiently inactivated by activated sludge alone. Mutagenicity was monitored with the Salmonella reversion assay conducted with the base-pair substitution detector strains, TA100 and YG1042, and the frame-shift detector strains, TA98 and YG1041, with and without rat liver S9. All dyes except for Congo Red (CR) were mutagenic with S9 activation. Assays conducted with the dyes indicated that only the azo dye Reactive Orange 16 (RO16) was mutagenic in both TA98 and TA100. Methyl Red and Disperse Blue 3 (DB3) were mutagenic in TA98, YG1041 and YG1042, while Reactive Black 5 was mutagenic in YG1041 and YG1042. Remazol Brilliant Blue R (RBBR), Crystal violet (CV) and Bromophenol Blue (BPB) were mutagenic only in TA98, but the toxicity of the latter two dyes complicated the evaluation of their mutagenicity. CR was not mutagenic in any of the tester strains. Biodegradation studies conducted with RO16 and DB3 indicated that the two-step biodegradation process reduced the mutagenic potential of RO16 and DB3 to a greater extent than activated sludge alone; the mutagenicity of the two dyes was reduced by 95.2% and 77.8%, respectively, by the two-step process. These data indicate that the combined biodegradation process may be useful for reducing the mutagenicity associated with wastewater from textile factories that contain recalcitrant dyes.


Environ Mol Mutagen


Malachová K,Pavlícková Z,Novotný C,Svobodová K,Lednická D,Musílková E




Has Abstract


2006-08-01 00:00:00












  • Novel transgenic rat for in vivo genotoxicity assays using 6-thioguanine and Spi- selection.

    abstract::Transgenic rodents are valuable models for investigating the genotoxicity of chemicals in vivo. Here, we report the establishment of a novel transgenic rat for genotoxicity analysis. In this model, about 10 copies of lambdaEG10 DNA carrying the gpt gene of E. coli and the red/gam genes of lambda phage are integrated p...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Hayashi H,Kondo H,Masumura K,Shindo Y,Nohmi T

    更新日期:2003-01-01 00:00:00

  • Simple and alpha,beta-unsaturated aldehydes: correct prediction of genotoxic activity through structure-activity relationship models.

    abstract::Aldehydes are widespread environmental and industrial compounds, able to stimulate a range of adverse health effects (e.g., general toxicity, allergenic reactions, mutagenicity, and carcinogenicity). We have previously presented quantitative structure-activity relationships (QSARs) for the genotoxicity of simple and a...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Benigni R,Conti L,Crebelli R,Rodomonte A,Vari' MR

    更新日期:2005-12-01 00:00:00

  • The influence of DNA repair by Ogt alkyltransferase on the distribution of alkylnitrosourea-induced mutations in Escherichia coli.

    abstract::To determine the influence of DNA repair by Ogt alkyltransferase on the distribution of alkylnitrosourea-induced mutations, we have analysed in Ogt-proficient and Ogt-deficient bacterial strains the DNA sequence changes of a total of 357 independent mutations occurring within the initial part of the lacl gene of Esche...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Vidal A,Abril N,Pueyo C

    更新日期:1997-01-01 00:00:00

  • Use of genetically engineered Salmonella typhimurium OY1002/1A2 strain coexpressing human cytochrome P450 1A2 and NADPH-cytochrome P450 reductase and bacterial O-acetyltransferase in SOS/umu assay.

    abstract::The major pathway of bioactivation of procarcinogenic heterocyclic aromatic amines (HCAs) is cytochrome P450 1A2 (CYP1A2)-catalyzed N-hydroxylation and subsequent esterification by O-acetyltransferase (O-AT). We have previously reported that an umu tester strain, Salmonella typhimurium OY1001/1A2, endogenously coexpre...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Aryal P,Terashita T,Guengerich FP,Shimada T,Oda Y

    更新日期:2000-01-01 00:00:00

  • DNA sequence analysis of X-ray induced Adh null mutations in Drosophila melanogaster.

    abstract::The mutational spectrum for 28 X-ray induced mutations and 2 spontaneous mutations, previously determined by genetic and cytogenetic methods, consisted of 20 multilocus deficiencies (19 induced and 1 spontaneous) and 10 intragenic mutations (9 induced and 1 spontaneous). One of the X-ray induced intragenic mutations w...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Mahmoud J,Fossett NG,Arbour-Reily P,McDaniel M,Tucker A,Chang SH,Lee WR

    更新日期:1991-01-01 00:00:00

  • Impact of transforming viruses on cellular mutagenesis, genome stability, and cellular transformation.

    abstract::It is estimated that 15% of all cancers are etiologically linked to viral infection. Specific cancers including adult T-cell leukemia, hepatocellular carcinoma, and uterine cervical cancer are associated with infection by human T-cell leukemia virus type I, hepatitis B virus, and high-risk human papilloma virus, respe...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: Gatza ML,Chandhasin C,Ducu RI,Marriott SJ

    更新日期:2005-03-01 00:00:00

  • Induction of micronuclei in human sperm-hamster egg hybrids at the two-cell stage after in vitro gamma-irradiation of human spermatozoa.

    abstract::The efficiency of the micronucleus test to assess radiation-induced chromosomal damage in human spermatozoa has been investigated. Micronuclei were scored in human sperm-hamster egg hybrids at the two-cell stage, after exposure of human spermatozoa to in vitro gamma-rays at doses of 0.00, 0.10, 0.25, 0.50, 1.00, 2.00,...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Tusell L,Alvarez R,Caballín MR,Genescà A,Miró R,Ribas M,Egozcue J

    更新日期:1995-01-01 00:00:00

  • Microsomal epoxide hydrolase (EPHX1) polymorphisms are associated with aberrant promoter methylation of ERCC3 and hematotoxicity in benzene-exposed workers.

    abstract::Benzene is an important industrial chemical and widespread environmental pollutant known to induce leukemia and other blood disorders. To be carcinogenic, benzene must be metabolized to produce toxic metabolites. To investigate whether single nucleotide polymorphisms (SNPs) in the metabolic enzyme genes are associated...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Xing C,Chen Q,Li G,Zhang L,Zheng M,Zou Z,Hou L,Wang QF,Liu X,Guo X

    更新日期:2013-07-01 00:00:00

  • Global regulatory requirements for mutagenicity assessment in the registration of industrial chemicals.

    abstract::Mutagenicity is an important toxicological endpoint that requires thorough evaluation during the industrial chemical registration process. Regulatory requirements for mutagenicity assessment in registration of industrial chemicals vary in geographic regions (and in some cases by intended application). Here we compile ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: Ji Z,Ball NS,LeBaron MJ

    更新日期:2017-06-01 00:00:00

  • The social environment and the epigenome.

    abstract::The genome is programmed by the epigenome. Two of the fundamental components of the epigenome are chromatin structure and covalent modification of the DNA molecule itself by methylation. DNA methylation patterns are sculpted during development and it has been a long held belief that they remain stable after birth in s...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: Szyf M,McGowan P,Meaney MJ

    更新日期:2008-01-01 00:00:00

  • DNA adduct formation in primary mouse embryo cells induced by 7H-dibenzo[c,g]carbazole and its organ-specific carcinogenic derivatives.

    abstract::The nuclease P1 modification of the 32P-postlabeling technique was used to study the biological activity of 7H-dibenzo[c,g]carbazole (DBC) and some of its derivatives, including N-methyldibenzo[c,g]carbazole (N-MeDBC), 5,9-dimethyldibenzo[c,g]carbazole (5,9-diMeDBC), 5,9,N-trimethyldibenzo[c,g]carbazole (5,9,N-triMeDB...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Gábelová A,Périn-Roussel O,Jounaidi Y,Périn F

    更新日期:1997-01-01 00:00:00

  • The use of benchmark dose uncertainty measurements for robust comparative potency analyses.

    abstract::The Benchmark Dose (BMD) method is the favored approach for quantitative dose-response analysis where uncertainty measurements are delineated between the upper (BMDU) and lower (BMDL) confidence bounds, or confidence intervals (CIs). Little has been published on the accurate interpretation of uncertainty measurements ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Wheeldon RP,Dertinger SD,Bryce SM,Bemis JC,Johnson GE

    更新日期:2021-01-11 00:00:00

  • Revisiting the rodent repairadox.

    abstract::Cultured rodent and human cells typically display similar clonal survival characteristics following exposure to ultraviolet light (UV). However, compared to human cells, cultured cells from mice, rats, and hamsters are generally deficient in excision repair of the most prominent DNA lesion produced by UV, the cyclobut...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: Hanawalt PC

    更新日期:2001-01-01 00:00:00

  • Evaluation of genetic damage in open-cast coal mine workers using the buccal micronucleus cytome assay.

    abstract::Coal is the largest fossil fuel source used for the generation of energy. However, coal extraction and its use constitute important pollution factors; thus, risk characterization and estimation are extremely important for the safety of coal workers and the environment. Candiota is located to the southeast of the state...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Rohr P,da Silva J,da Silva FR,Sarmento M,Porto C,Debastiani R,Dos Santos CE,Dias JF,Kvitko K

    更新日期:2013-01-01 00:00:00

  • Aurora A, centrosome structure, and the centrosome cycle.

    abstract::The centrosome, also known as the microtubule organizing center of the cell, is a membrane-less organelle composed of a pair of barrel-shaped centrioles surrounded by electron-dense pericentriolar material. The centrosome progresses through the centrosome cycle in step with the cell cycle such that centrosomes are dup...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: Lukasiewicz KB,Lingle WL

    更新日期:2009-10-01 00:00:00

  • Mutagenicity and clastogenicity of gas stove emissions in bacterial and plant tests.

    abstract::The aim of this research was to study the gaseous and particulate emissions of genotoxic substances during cooking with two types of methane stoves (a new one and an old one). The particulates were sampled both with a cascade impactor air sampler and an impinger with ice trap and analyzed by two bacterial mutagenicity...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Monarca S,Zanardini A,Feretti D,Falistocco E,Antonelli P,Resola S,Moretti M,Villarini M,Nardi G

    更新日期:1998-01-01 00:00:00

  • HUman MicroNucleus project: international database comparison for results with the cytokinesis-block micronucleus assay in human lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei.

    abstract::Micronucleus (MN) expression in peripheral blood lymphocytes is well established as a standard method for monitoring chromosome damage in human populations. The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus (CBMN) assay and participating in the HUMN (HUman Micro...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,多中心研究


    authors: Bonassi S,Fenech M,Lando C,Lin YP,Ceppi M,Chang WP,Holland N,Kirsch-Volders M,Zeiger E,Ban S,Barale R,Bigatti MP,Bolognesi C,Jia C,Di Giorgio M,Ferguson LR,Fucic A,Lima OG,Hrelia P,Krishnaja AP,Lee TK,Migliore L

    更新日期:2001-01-01 00:00:00

  • Is chronic use of hydroxyurea safe for patients with sickle cell anemia? An account of genotoxicity and mutagenicity.

    abstract::Sickle cell anemia (SCA) is a hereditary hematological disease that is characterized by a point mutation in the beta globin S gene and has no specific treatment; hydroxyurea (HU) is the only therapeutic agent used in clinical practice. In the present study, we evaluated the deoxyribonucleic acid (DNA) damage index (DI...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 信件


    authors: Maia Filho PA,Pereira JF,Almeida Filho TP,Cavalcanti BC,Sousa JC,Lemes RPG

    更新日期:2019-04-01 00:00:00

  • In vitro effects of 1-MHz ultrasound on the mitotic spindle.

    abstract::The effects of ultrasound on the cytoskeleton, comprising microtubules, had been studied decades ago. Nonetheless, very little attention has been paid to the effects of ultrasound on the mitotic spindle, which is also formed by microtubules. In this study, we treated human fibroblasts and human cancer cells (HeLa and ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Udroiu I,Coluzzi E,Bedini A,Giliberti C,Palomba R,Sgura A

    更新日期:2019-08-01 00:00:00

  • Evaluation of DNA intercalation potential of pharmaceuticals and other chemicals by cell-based and three-dimensional computational approaches.

    abstract::To what extent noncovalent chemical-DNA interactions, in particular weak nonbonded DNA intercalation, contribute to genotoxic responses in mammalian cells has not been fully elucidated. Moreover, with the exception of predominantly flat, multiple-fused-ring structures, our ability to predict intercalation ability of n...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Snyder RD,Ewing DE,Hendry LB

    更新日期:2004-01-01 00:00:00

  • Influence of DNA repair gene polymorphisms on the initial repair of MMS-induced DNA damage in human lymphocytes as measured by the alkaline comet assay.

    abstract::We have applied the alkaline comet assay to study the functional impact of gene polymorphisms in base excision repair (APEX1 Asp148Glu, XRCC1 Arg194Trp, XRCC1 Arg399Gln) and homologous recombination repair (XRCC3 Thr241Met, NBS1 Glu185Gln), two pathways that play crucial roles in the repair of DNA damage induced by me...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Ryk C,Routledge MN,Allan JM,Wild CP,Kumar R,Lambert B,Hou S

    更新日期:2008-12-01 00:00:00

  • Comparison of mutagenicity and calf thymus DNA adducts formed by the particulate and semivolatile fractions of vehicle exhausts.

    abstract::In this study we compared the ability of extractable organic material from particulate and semivolatile fractions of gasoline emission to induce mutations in bacteria and form adducts with calf thymus (CT) DNA with corresponding data obtained from diesel exhaust. Exhaust particles from gasoline-powered passenger cars ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Pohjola SK,Lappi M,Honkanen M,Savela K

    更新日期:2003-01-01 00:00:00

  • Analysis of the transcriptome in molecular epidemiology studies.

    abstract::The human transcriptome is complex, comprising multiple transcript types, mostly in the form of non-coding RNA (ncRNA). The majority of ncRNA is of the long form (lncRNA, ≥ 200 bp), which plays an important role in gene regulation through multiple mechanisms including epigenetics, chromatin modification, control of tr...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: McHale CM,Zhang L,Thomas R,Smith MT

    更新日期:2013-08-01 00:00:00

  • Mutagenicity of the human carcinogen treosulphan, and its hydrolysis product, dl-1,2:3,4-diepoxybutane in mammalian cells.

    abstract::The cytotoxicity and mutagenicity of the human carcinogen, treosulphan, and its hydrolysis product, dl-1,2:3,4-diepoxybutane (DEB), were studied in Chinese hamster ovary, AS52, cells. Treosulphan (0.1-1.0 mM) is toxic and mutagenic at the gpt locus. A strong pH dependence was noted. DEB is cytotoxic and mutagenic at a...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Zhu S,Zeiger E

    更新日期:1993-01-01 00:00:00

  • The SOS system: A complex and tightly regulated response to DNA damage.

    abstract::Genomes of all living organisms are constantly threatened by endogenous and exogenous agents that challenge the chemical integrity of DNA. Most bacteria have evolved a coordinated response to DNA damage. In Escherichia coli, this inducible system is termed the SOS response. The SOS global regulatory network consists o...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章,评审


    authors: Maslowska KH,Makiela-Dzbenska K,Fijalkowska IJ

    更新日期:2019-05-01 00:00:00

  • Genetic polymorphisms and susceptibility to childhood acute lymphoblastic leukemia.

    abstract::Acute lymphoblastic leukemia (ALL) is the most common form of pediatric cancer. Although exposure to environmental agents appears to predispose individuals to this disease, little attention has been paid to the role of genetic susceptibility to environmental exposures in the etiology of childhood ALL. The enzymes GSTM...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Canalle R,Burim RV,Tone LG,Takahashi CS

    更新日期:2004-01-01 00:00:00

  • Human erythrocyte PIG-A assay: an easily monitored index of gene mutation requiring low volume blood samples.

    abstract::This laboratory has previously described a method for scoring the incidence of rodent blood Pig-a mutant phenotype erythrocytes using immunomagnetic separation in conjunction with flow cytometric analysis (In Vivo MutaFlow®). The current work extends this approach to human blood. The frequencies of CD59- and CD55-nega...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Dertinger SD,Avlasevich SL,Bemis JC,Chen Y,MacGregor JT

    更新日期:2015-05-01 00:00:00

  • Micronuclei formation in somatic cells of mice exposed to 50-Hz electric fields.

    abstract::Male Swiss mice are tested under uniform 50-Hz electric field intensities of 100, 170, 220, and 290 kV/m. These values on the basis of equal induced current density are equivalent to the case of a human exposed to field intensities of nearly 8, 14, 18, and 24 kV/m, respectively. The latter values may be found beneath ...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: el Nahas SM,Oraby HA

    更新日期:1989-01-01 00:00:00

  • Alterations of plasma antioxidants and mitochondrial DNA mutation in hair follicles of smokers.

    abstract::The effects of long-term smoking on mitochondrial DNA (mtDNA) deletions in hair follicles were investigated in subjects with different antioxidant capacity. Twenty-two male smokers with a smoking index of greater than 5 pack-years and without any known systemic diseases were recruited for this study. Forty healthy non...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Liu CS,Chen HW,Lii CK,Tsai CS,Kuo CL,Wei YH

    更新日期:2002-01-01 00:00:00

  • Evaluation of cytotoxicity and genotoxicity induced by oleic acid-coated iron oxide nanoparticles in human astrocytes.

    abstract::Iron oxide nanoparticles (ION) are gaining importance as diagnostic and therapeutic tool of central nervous system diseases. Although oleic acid-coated ION (O-ION) have been described as stable and biocompatible, their potential neurotoxicity was scarcely evaluated in human nervous cells so far. The primary aim of thi...

    journal_title:Environmental and molecular mutagenesis

    pub_type: 杂志文章


    authors: Fernández-Bertólez N,Costa C,Brandão F,Duarte JA,Teixeira JP,Pásaro E,Valdiglesias V,Laffon B

    更新日期:2019-12-01 00:00:00