Understanding of and attitude to xenotransplantation among Italian university students: impact of a 3-yr university course.


BACKGROUND:Young people, and students in particular, generally have a positive attitude to xenotransplantation. We reported previously that university students attending their first year approved of the idea of transplanting animal organs into humans. The aim of this study was to evaluate the impact of a 3-yr course at various faculties on the university students' understanding of and attitude to xenotransplantation. METHODS:University students in their fourth year at five different faculties of Padua University (Italy), who had previously been surveyed in exactly the same way 3 yr earlier, were re-administered an anonymous 19-item questionnaire on their attitude to xenotransplantation. RESULTS:A total of 453 students completed the fourth year questionnaire (84 males, 369 females; mean age 24.6 yr, range 21-36 yr). Students were attending courses in Medicine (22.1%), Veterinary Science (16.5%), Agriculture (10.4%), Educational Sciences (22.1%) and Psychology (28.9%). In their fourth year, 85% of the students knew that animal organs could, at some stage, be transplanted into humans, 81.5% approved of this idea and 84% would accept an animal organ to save their life if necessary (these percentages were 88%, 78% and 76%, respectively 3 yr earlier). A significantly larger proportion of the students who approved of xenotransplantation were attending science courses rather than art courses, while no differences emerged as regards gender. Those who refused xenotransplantation justified their position mainly on ethical-moral (41.7%) and immunological (35.7%) grounds. CONCLUSION:As in their first year, so too in their fourth, University students were well informed about the feasibility of transplanting animal organs into human beings and those attending science courses were more likely to accept this idea than art students. Approval of xenotransplantation was much the same among fourth year males and females, whereas in the first year, male students had approved more than female students.






Canova D,De Bona M,Rumiati R,Masier A,Ermani M,Naccarato R,Cozzi E,Ancona E,Burra P




Has Abstract


2006-05-01 00:00:00














  • Cyclophosphamide, but not CTLA4Ig, prolongs survival of fetal pig islet grafts in anti-T cell monoclonal antibody-treated NOD mice.

    abstract::Fetal pig islets, xenografted after organ culture into non-immunosuppressed prediabetic NOD mice, are rejected within 10 days. Immunosuppression with anti-T cell (anti-CD4 and anti-CD3) monoclonal antibodies alone is highly effective in delaying graft rejection in this discordant model, but rejection eventually occurs...


    pub_type: 杂志文章


    authors: Koulmanda M,Kovarik J,Mandel TE

    更新日期:1998-08-01 00:00:00

  • T-cell-mediated immunological barriers to xenotransplantation.

    abstract::Xenotransplantion remains the most viable option for significant expansion of the donor organ pool in clinical transplantation. With the advent of nuclear transfer technologies, the production of transgenic swine has become a possibility. These animals have allowed transplant investigators to overcome humoral mechanis...


    pub_type: 杂志文章,评审


    authors: Scalea J,Hanecamp I,Robson SC,Yamada K

    更新日期:2012-01-01 00:00:00

  • Characterization and quantification of porcine circulating endothelial cells.

    abstract:BACKGROUND:Endothelial damage is a critical step in the development of (xeno) transplantation-related and cardiovascular pathology. In humans, the amount of circulating endothelial cells (CEC) correlates to disease intensity and functions as a valuable damage marker. While (xeno) transplantation and cardiovascular rese...


    pub_type: 杂志文章


    authors: Post IC,Weenink RP,van Wijk AC,Heger M,Böing AN,van Hulst RA,van Gulik TM

    更新日期:2013-01-01 00:00:00

  • Rapid loss of intraportally transplanted islets: an overview of pathophysiology and preventive strategies.

    abstract::Islets isolated from multiple pancreas donors are often necessary to achieve euglycemia in type 1 diabetic patients treated by islet allotransplantation. This increases the burden on the limited pool of donor organs. After infusion into the portal vein, a substantial percentage of islets are lost in the immediate post...


    pub_type: 杂志文章,评审


    authors: van der Windt DJ,Bottino R,Casu A,Campanile N,Cooper DK

    更新日期:2007-07-01 00:00:00

  • Regulation of xenogeneic porcine pancreatic islets.

    abstract::The use of xenogeneic porcine pancreatic islets has been shown to be a potentially promising alternative to using human allogeneic islets to treat insulin-dependent type 1 diabetes (T1D). This article provides an overview of the existing FDA regulatory framework that would be applied to the regulation of clinical tria...


    pub_type: 杂志文章,评审


    authors: Arcidiacono JA,Evdokimov E,Lee MH,Jones J,Rudenko L,Schneider B,Snoy PJ,Wei CH,Wensky AK,Wonnacott K

    更新日期:2010-09-01 00:00:00

  • Diabetes induction by total pancreatectomy in minipigs with simultaneous splenectomy: a feasible approach for advanced diabetes research.

    abstract:BACKGROUND:Safe and reliable diabetes models are a key prerequisite for advanced preclinical studies on diabetes. Chemical induction is the standard model of diabetes in rodents and also widely used in large animal models of non-human primates and minipigs. However, uncertain efficacy, the potential of beta-cell regene...


    pub_type: 杂志文章


    authors: Heinke S,Ludwig B,Schubert U,Schmid J,Kiss T,Steffen A,Bornstein S,Ludwig S

    更新日期:2016-09-01 00:00:00

  • Pig islet xenografts are resistant to autoimmune destruction by non-obese diabetic recipients after anti-CD4 treatment.

    abstract::In addition to providing a large source of donor tissue, xenogeneic islet transplantation might avoid recurrent autoimmunity in patients with type 1 diabetes. To examine this possibility further, xenogeneic pig islets were transplanted into recipient mice in the presence or absence of autoimmunity. Spontaneously, non-...


    pub_type: 杂志文章


    authors: Koulmanda M,Qipo A,Smith RN,Auchincloss H Jr

    更新日期:2003-03-01 00:00:00

  • Glucose metabolism in pigs expressing human genes under an insulin promoter.

    abstract:BACKGROUND:Xenotransplantation of porcine islets can reverse diabetes in non-human primates. The remaining hurdles for clinical application include safe and effective T-cell-directed immunosuppression, but protection against the innate immune system and coagulation dysfunction may be more difficult to achieve. Islet-ta...


    pub_type: 杂志文章


    authors: Wijkstrom M,Bottino R,Iwase H,Hara H,Ekser B,van der Windt D,Long C,Toledo FG,Phelps CJ,Trucco M,Cooper DK,Ayares D

    更新日期:2015-01-01 00:00:00

  • Pigs expressing the human inhibitory ligand PD-L1 (CD 274) provide a new source of xenogeneic cells and tissues with low immunogenic properties.

    abstract:BACKGROUND:The programmed cell death-1 (PD-1, CD279)/PD-Ligand1 (PD-L1, CD274) receptor system is crucial for controlling the balance between immune activation and induction of tolerance via generation of inhibitory signals. Expression of PD-L1 is associated with reduced immunogenicity and renders cells and tissues to ...


    pub_type: 杂志文章


    authors: Buermann A,Petkov S,Petersen B,Hein R,Lucas-Hahn A,Baars W,Brinkmann A,Niemann H,Schwinzer R

    更新日期:2018-09-01 00:00:00

  • Release of pig leukocytes and reduced human NK cell recruitment during ex vivo perfusion of HLA-E/human CD46 double-transgenic pig limbs with human blood.

    abstract:BACKGROUND:In pig-to-human xenotransplantation, interactions between human natural killer (NK) cells and porcine endothelial cells (pEC) are characterized by recruitment and cytotoxicity. Protection from xenogeneic NK cytotoxicity can be achieved in vitro by the expression of the non-classical human leukocyte antigen-E...


    pub_type: 杂志文章


    authors: Puga Yung G,Bongoni AK,Pradier A,Madelon N,Papaserafeim M,Sfriso R,Ayares DL,Wolf E,Klymiuk N,Bähr A,Constantinescu MA,Voegelin E,Kiermeir D,Jenni H,Rieben R,Seebach JD

    更新日期:2018-01-01 00:00:00

  • Ultrastructural alterations in human blood leukocytes induced by porcine circovirus type 1 infection.

    abstract:BACKGROUND:Swine infectious pathogens, especially viruses, represent a potential public health risk associated with the use of pig tissues for xenotransplantation in humans. We hypothesized that porcine circovirus type I (PCV-1) may infect human mononuclear cells, resulting in ultrastructural alterations of the target ...


    pub_type: 杂志文章


    authors: Arteaga-Troncoso G,Guerra-Infante F,Rosales-Montaño LM,Díaz-García FJ,Flores-Medina S

    更新日期:2005-11-01 00:00:00

  • Cytokine-inducible promoters to drive dynamic transgene expression: The "Smart Graft" strategy.

    abstract:BACKGROUND:Ubiquitous expression of T-cell regulatory transgenes such as the cytotoxic T lymphocyte-associated antigen 4 (CTLA4) or the high-affinity variant LEA29Y improves xeno graft survival. Such donor pigs are however immunocompromised and susceptible to infection. Continous high expression of CTLA4 or LEA29Y in t...


    pub_type: 杂志文章


    authors: Fischer A,Manske K,Seissler J,Wohlleber D,Simm N,Wolf-van Buerck L,Knolle P,Schnieke A,Fischer K

    更新日期:2020-11-01 00:00:00

  • ABO-incompatible live donor renal transplantation using blood group A/B carbohydrate antigen immunoadsorption and anti-CD20 antibody treatment.

    abstract:BACKGROUND:Blood group ABO-incompatible live donor (LD) renal transplantation may provide a significant source of organs. We report the results of our first 14 cases of ABO-incompatible LD renal transplantation using specific anti-A/B antibody (Ab) immunoadsorption (IA) and anti-CD20 monoclonal Ab (mAb) treatment. PATI...


    pub_type: 杂志文章


    authors: Nordén G,Briggs D,Cockwell P,Lipkin G,Mjörnstedt L,Mölne J,Ready A,Rydberg L,Samuelsson O,Svalander CT,Breimer ME

    更新日期:2006-03-01 00:00:00

  • Increased human tumor necrosis factor-α levels induce procoagulant change in porcine endothelial cells in vitro.

    abstract:BACKGROUND:Intravascular thrombosis and systemic coagulation abnormalities are major hurdles to successful xenotransplantation and are signs of acute humoral rejection. Increased expression of tissue factor (TF) is associated with the development of microvascular thrombosis in xenografts. To develop an effective strate...


    pub_type: 杂志文章


    authors: Lee KG,Lee H,Ha JM,Lee YK,Kang HJ,Park CG,Kim SJ

    更新日期:2012-05-01 00:00:00

  • Real-time assessment of encapsulated neonatal porcine islets prior to clinical xenotransplantation.

    abstract:BACKGROUND:Porcine islet transplantation is emerging as an attractive option for the treatment of patients with type 1 diabetes, with the possibility of providing islets of higher and more consistent quality and in larger volumes than available from human pancreata. The use of encapsulated neonatal porcine islets (ENPI...


    pub_type: 杂志文章


    authors: Kitzmann JP,Law L,Shome A,Muzina M,Elliott RB,Mueller KR,Schuurman HJ,Papas KK

    更新日期:2012-11-01 00:00:00

  • Jewish, Christian and Muslim theological perspectives about xenotransplantation.

    abstract:BACKGROUND:This paper is based on a theological symposium presented at the International Xenotransplantation Association's 14th Congress held in Baltimore, MD, September, 2017. METHODS:The information explores the Jewish, Christian and Muslim theological perceptions and perspectives about cross-species (ie pig-to-huma...


    pub_type: 杂志文章


    authors: Paris W,Seidler RJH,FitzGerald K,Padela AI,Cozzi E,Cooper DKC

    更新日期:2018-05-01 00:00:00

  • Toward a solution for cardiac failure in the newborn.

    abstract::The newborn infant with severe cardiac failure owed to congenital structural heart disease or cardiomyopathy poses a daunting therapeutic challenge. The ideal solution for both might be cardiac transplantation if availability of hearts was not limiting and if tolerance could be induced, obviating toxicity of immunosup...


    pub_type: 杂志文章,评审


    authors: Platt JL,West LJ,Chinnock RE,Cascalho M

    更新日期:2018-11-01 00:00:00

  • Viral opportunistic infections in Mauritian cynomolgus macaques undergoing allogeneic stem cell transplantation mirror human transplant infectious disease complications.

    abstract::Allogeneic hematopoietic stem cell transplantation (HSCT) and xenotransplantation are accompanied by viral reactivations and virus-associated complications resulting from immune deficiency. Here, in a Mauritian cynomolgus macaque model of fully MHC-matched allogeneic HSCT, we report reactivations of cynomolgus polyoma...


    pub_type: 杂志文章


    authors: Wu HL,Weber WC,Shriver-Munsch C,Swanson T,Northrup M,Price H,Armantrout K,Robertson-LeVay M,Reed JS,Bateman KB,Mahyari E,Thomas A,Junell SL,Hobbs TR,Martin LD,MacAllister R,Bimber BN,Slifka MK,Legasse AW,Moats C,A

    更新日期:2020-07-01 00:00:00

  • Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.

    abstract::We describe in this report the production and characterization of monoclonal antibodies (mAb) to the swine homologues of CD11a and CD18 antigens, and their use for phenotypic and functional analysis of porcine leukocytes. Monoclonal antibodies BL1H8 and BL2F1 precipitated two bands of approximately 170 and 95 kDa, whe...


    pub_type: 杂志文章


    authors: Alvarez B,Doménech N,Alonso F,Sánchez C,Gómez del Moral M,Ezquerra A,Domínguez J

    更新日期:2000-11-01 00:00:00

  • Xenograft rejection of fetal porcine islet-like cell clusters in the rat: effects of active and passive immunization.

    abstract::The process of islet xenograft rejection is still poorly understood. To elucidate further possible mechanism(s) involved in xenograft rejection, the effect of different immunization protocols was investigated. Fetal porcine islet-like cell clusters (ICCs) were transplanted under the kidney capsule in otherwise untreat...


    pub_type: 杂志文章


    authors: Korsgren O,Wallgren AC,Satake M,Karlsson-Parra A

    更新日期:1999-11-01 00:00:00

  • Initial experience with the human anti-human CD154 monoclonal antibody, ABI793, in pig-to-baboon xenotransplantation.

    abstract:BACKGROUND:ABI793 (ABI) is a human monoclonal antibody (mAb) specific for human CD154. To assess the suitability of ABI for baboon transplantation studies, we carried out in vitro studies to determine ABI's reactivity with baboon cells expressing CD154, performed in vivo pharmacokinetic studies in two baboons, and test...


    pub_type: 杂志文章


    authors: Knosalla C,Ryan DJ,Moran K,Gollackner B,Schuler W,Sachs DH,Awwad M,Schuurman HJ,Cooper DK

    更新日期:2004-07-01 00:00:00

  • Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves.

    abstract:BACKGROUND:The present study reports the development of a sensitive dot blot protocol for determining the level of preformed antibodies against porcine heart valve tissue derived from wild-type (WT) and α-Gal-KO (GGTA1-KO) pigs in human sera. METHODS:The assay uses decellularized and solubilized heart valve tissue; an...


    pub_type: 杂志文章


    authors: Smart I,Goecke T,Ramm R,Petersen B,Lenz D,Haverich A,Niemann H,Hilfiker A

    更新日期:2020-09-17 00:00:00

  • The distribution of porcine pancreatic beta-cells at ages 5, 12 and 24 weeks.

    abstract::Islet transplantation is a potential treatment for diabetes mellitus and porcine pancreata may provide a readily available source of islets. The size, number and distribution of islets within the pancreas may influence the choice of age of donor for xenotransplantation. Samples (n = 3 per age group) from the dorsal an...


    pub_type: 杂志文章


    authors: Jay TR,Heald KA,Carless NJ,Topham DE,Downing R

    更新日期:1999-05-01 00:00:00

  • An overview of the role of society and risk in xenotransplantation.

    abstract:BACKGROUND:Over time, the notion of public has evolved. While the concept of public was initially conceived of as a single undifferentiated entity, the common understanding is now that a variety of differentiated, multifaceted and multiple public(s) can be constructed for different purposes. This is equally true in xen...


    pub_type: 杂志文章,评审


    authors: Sobbrio P,Jorqui M

    更新日期:2014-11-01 00:00:00

  • Pig endogenous retroviruses and xenotransplantation.

    abstract::Xenotransplantation of porcine organs might provide an unlimited source of donor organs to treat endstage organ failure diseases in humans. However, pigs harbour retroviruses with unknown pathogenic potential as an integral part of their genome. While until recently the risk of interspecies transmission of these porci...


    pub_type: 杂志文章,评审


    authors: Blusch JH,Patience C,Martin U

    更新日期:2002-07-01 00:00:00

  • Epigenetic biomarkers indicate islet cell death in xenotransplantation.

    abstract:BACKGROUND:Xenotransplantation of porcine islets has emerged in recent decades as a potential treatment for type 1 diabetes (T1D). Current methods of detection, indicative of successful engraftment, occur downstream of actual islet death. Epigenetic biomarkers can be detected in circulating cell-free DNA (cfDNA) to pro...


    pub_type: 杂志文章


    authors: Faulk C,Mueller KR,Cheishvili D,Colwell M,Pepin AS,Syzf M,Hering BJ,Burlak C

    更新日期:2020-03-01 00:00:00

  • Absence of humoral and cellular alloreactivity in baboons sensitized to pig antigens.

    abstract:AIM:to study whether sensitization to pig antigens results in humoral and/or cellular sensitization to alloantigens in baboons, and thus increases the risks of organ allotransplantation after xenotransplantation. Serum from baboons that were naive (n = 4), sensitized to Gal alpha 1,3Gal (Gal) antigens (n = 2), or sensi...


    pub_type: 杂志文章


    authors: Baertschiger RM,Dor FJ,Prabharasuth D,Kuwaki K,Cooper DK

    更新日期:2004-01-01 00:00:00

  • Pig peripheral blood mononuclear cells are directly associated with the thrombotic microangiopathy that complicates the induction of chimerism in pig-to-baboon xenotransplantation.

    abstract::The infusion of large numbers of porcine cells into primates in order to induce specific immunologic tolerance by mixed hematopoietic chimerism, results in thrombotic microangiopathy that can be fatal. For this reason, it is important to study in vitro the interaction of primate endothelial cells with pig cells. We sh...


    pub_type: 杂志文章


    authors: Benatuil L,Fernandez AZ,Apitz-Castro R,Romano E

    更新日期:2002-05-01 00:00:00

  • Transplantation of human CD34+ stem cells from umbilical cord blood to rats with thioacetamide-induced liver cirrhosis.

    abstract:BACKGROUND:Liver fibrosis results from accumulation of extracellular matrix components and is associated with many chronic hepatic diseases. There is to date no specific therapy for this disease, and patients receive treatment for its associated complications. Specific progenitor cells, known as oval cells, are present...


    pub_type: 杂志文章


    authors: Sáez-Lara MJ,Frecha C,Martín F,Abadía F,Toscano M,Gil A,Fontana L

    更新日期:2006-11-01 00:00:00

  • History of xenotransplantation.

    abstract::The present historical review reports the clinical experiences of transplantations from animal to human. The first transplantation attempts were made without any knowledge of the species barrier. The pioneers of xenotransplantation realized xenotransfusions as early as the 16th century, then cell and tissue xenotransp...


    pub_type: 历史文章,杂志文章


    authors: Deschamps JY,Roux FA,Saï P,Gouin E

    更新日期:2005-03-01 00:00:00