Complexity of Pseudomonas aeruginosa infection in cystic fibrosis: combined results from esterase electrophoresis and rDNA restriction fragment length polymorphism analysis.

Abstract:

:Esterase electrophoretic typing and restriction fragment length polymorphism of ribosomal DNA regions (ribotyping) were used to differentiate 102 Pseudomonas aeruginosa clinical isolates obtained from chronic lung infection in 23 patients with cystic fibrosis (CF) and two reference strains (including the type strain ATCC 10145). Twenty-five zymotypes were obtained with the former method and 16 ribotypes with the latter. Combination of the two typing systems led to the finding of 30 different types. Our data highlights the physiopathological complexity of P. aeruginosa infection in CF as, in six individual cases, several types were found among isolates from a given patient. On the other hand, two unique types were found in two and three patients respectively, raising the possibility of cross-infections.

journal_name

Epidemiol Infect

authors

Denamur E,Picard B,Goullet P,Bingen E,Lambert N,Elion J

doi

10.1017/s0950268800067595

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

531-9

issue

3

eissn

0950-2688

issn

1469-4409

journal_volume

106

pub_type

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