Development and validation of liquid chromatographic and UV derivative spectrophotometric methods for the determination of famciclovir in pharmaceutical dosage forms.


:A high-performance liquid chromatographic method and a UV derivative spectrophotometric method for the determination of famciclovir, a highly active antiviral agent, in tablets were developed in the present work. The various parameters, such as linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation were studied according to International Conference on Harmonization guidelines. HPLC was carried out by using the reversed-phase technique on an RP-18 column with a mobile phase composed of 50 mM monobasic phosphate buffer and methanol (50 : 50; v/v), adjusted to pH 3.05 with orthophosphoric acid. The mobile phase was pumped at a flow rate of 1 ml/min and detection was made at 242 nm with UV dual absorbance detector. The first derivative UV spectrophotometric method was performed at 226.5 nm. Statistical analysis was done by Student's t-test and F-test, which showed no significant difference between the results obtained by the two methods. The proposed methods are highly sensitive, precise and accurate and therefore can be used for its Intended purpose.


Srinubabu G,Sudharani B,Sridhar L,Rao JS




Has Abstract


2006-06-01 00:00:00














  • Steroidal glycosides from the fruits of Solanum viarum.

    abstract::Three new steroidal glycosides, named solaviasides A, B, and C, have been isolated from the fruits of Solanum viarum DUNAL (syn. S. khasianum var. chatterjeeanum, Solanaceae), along with seven known ones. Their chemical structures were determined on the basis of spectroscopic data and chemical evidence. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ono M,Kakiuchi T,Ebisawa H,Shiono Y,Nakamura T,Kai T,Ikeda T,Miyashita H,Yoshimitsu H,Nohara T

    更新日期:2009-06-01 00:00:00

  • Synthesis of optically active NC-1800, a therapeutic agent for urinary disturbance.

    abstract::A new synthetic method for chiral oxazolidinone derivatives, therapeutic agents for treating urinary disturbance, is described. The condensed compound obtained from chiral 1-amino-3-phenyl-2-propanol and 1-phenyl-3-morpholino-1-propanone was reduced with Me4NBH(OAc)3 to give the intermediate, 1-(3-morpholino-1-phenylp...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yoshida S,Yamamoto M,Mochizuki N,Nohira H

    更新日期:1999-08-01 00:00:00

  • Photoinduced upregulation of calcitonin gene-related peptide in A549 cells through HNO release from a hydrophilic photocontrollable HNO donor.

    abstract::Nitroxyl (HNO), a one-electron-reduced form of nitric oxide, has various biological activities, including a cardioprotective effect. Here, we first synthesized another, more hydrophilic photocontrollable HNO donor (3), which can release HNO in a spatially and temporally controlled manner, and then examined the propert...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsuo K,Nakagawa H,Adachi Y,Kameda E,Aizawa K,Tsumoto H,Suzuki T,Miyata N

    更新日期:2012-01-01 00:00:00

  • Three new sesquiterpenoid glucosides of Ficus pumila fruit.

    abstract::As the glycosyl constituents of Ficus pumila L. fruits (Moraceae), three new sesquiterpenoid glucosides, pumilasides A, B and C were isolated together with benzyl beta-D-glucopyranoside, (E)-2-methyl-2-butenyl beta-D-glucopyranoside and rutin. Their structures were characterized as (1S,4S,5R,6R,7S,10S)-1,4,6-trihydrox...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kitajima J,Kimizuka K,Tanaka Y

    更新日期:2000-01-01 00:00:00

  • Transcellular transport of low density lipoprotein through cultured newborn rat skin epidermal cell monolayer.

    abstract::Epidermal cells from newborn rat skin were cultured on type IV collagen-coated Millipore filter, and the transport of low density lipoprotein (LDL) labeled with Rhodamine B isothiocyanate (RB-LDL) through the cultured cell layer was examined. The transport of RB-LDL was dependent on temperature and biological energy. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ohkura K,Terada H

    更新日期:1991-02-01 00:00:00

  • Tyrosine formation from phenylalanine by ultraviolet irradiation.

    abstract::When phenylalanine was irradiated at ultraviolet (UV) light, p-tyrosine, m-tyrosine and o-tyrosine were identified as hydroxylated products. From p-tyrosine and m-tyrosine, the formation of L-3,4-dihydroxyphenylalanine (DOPA) was observed. The hydroxylation of phenylalanine was prevented by radical scavengers, e.g., c...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ishimitsu S,Fujimoto S,Ohara A

    更新日期:1990-05-01 00:00:00

  • Screening for molluscicidal activity in crude drugs.

    abstract::Thirty-four extracts of crude drugs and medicinal plants have been screened for activity against Oncomelania nosophora, the intermediate host of the Japanese strain of Schistosoma japonicum. Strong molluscicidal activity was found in the MeOH extract of Anemarrhenae Rhizoma. Although timosaponin A-III, one of the main...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takeda O,Tanaka S,Yamasaki K,Kohda H,Iwanaga Y,Tsuji M

    更新日期:1989-04-01 00:00:00

  • On the interaction of doxorubicin with oleate ions: fluorescence spectroscopy and liquid-liquid extraction study.

    abstract::Increase of lipophilicity of cationic doxorubicin (DOX) by its association with a fatty acid ion is of interest for pharmaceutical formulations and could have an impact on the drug delivery into cancer cells. On the basis of spectroscopic analysis of intrinsic DOX fluorescence, this study provides an experimental evid...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Munnier E,Tewes F,Cohen-Jonathan S,Linassier C,Douziech-Eyrolles L,Marchais H,Soucé M,Hervé K,Dubois P,Chourpa I

    更新日期:2007-07-01 00:00:00

  • Tryptanthrin-loaded nanoparticles for delivery into cultured human breast cancer cells, MCF7: the effects of solid lipid/liquid lipid ratios in the inner core.

    abstract::Tryptanthrin is an ancient medicine which recently was also found to have a function of downregulating multidrug resistance (MDR). However, tryptanthrin is insoluble in water, which limits its availability for delivery into cancer cells. There is a need to improve delivery systems to increase the inhibition of MDR. Th...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Fang YP,Lin YK,Su YH,Fang JY

    更新日期:2011-01-01 00:00:00

  • Percutaneous absorption of clonazepam in rabbit.

    abstract::The percutaneous (p.c.) absorption of clonazepam (CZP), an antiepileptic drug, was investigated in rabbits. CZP was efficiently absorbed from a gel ointment (0.5% CZP, 1g, 9 cm2) with Azone and therapeutic plasma concentrations were maintained for 27 h. The bioavailability of CZP from the gel ointment was 47.2 +/- 3.1...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ogiso T,Ito Y,Iwaki M,Yamamoto Y

    更新日期:1989-02-01 00:00:00

  • Synthesis and biological evaluation of 5-arylfuro[2,3-d]pyrimidines as novel dihydrofolate reductase inhibitors.

    abstract::A series of about fifty novel 5-arylfuro[2,3-d]pyrimidine derivatives were synthesized as potential inhibitors of dihydrofolate reductase (DHFR) arising from different species. Weak enzyme inhibition was observed for most of the compounds, with only a few reaching IC50 values less than 30 microM. With regards to antib...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wahid F,Monneret C,Dauzonne D

    更新日期:1999-02-01 00:00:00

  • Evaluation of N-bromosuccinimide as a new analytical reagent for the spectrophotometric determination of fluoroquinolone antibiotics.

    abstract::Analytical studies were carried out, for the first time, to evaluate the use of N-bromosuccinimide (NBS) as an analytical reagent for the spectrophotometric determination of eleven therapeutically important fluoroquinolone antibiotics (FQA). The procedures involved the reaction of the FQA with NBS and subsequent measu...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Askal H,Refaat I,Darwish I,Marzouq M

    更新日期:2007-11-01 00:00:00

  • First synthesis of a alpha-trifluoromethyl allenol ether via the Julia-Lythgoe process.

    abstract::Alpha-trifluoromethyl allenol ethers 9a-e were prepared in moderate to good yields by the Julia-Lythgoe process using beta-ethoxy-beta-trifluoromethyl vinylic sulfone 3. Several reactions of 9c were examined to give alpha,beta-unsaturated trifluoromethyl ketone derivatives 11 and 12. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yoshimatsu M,Hibino M

    更新日期:2000-09-01 00:00:00

  • A novel preparation method for microspheres of water soluble polymers using polypropyleneglycol as the dispersion medium.

    abstract::Polypropyleneglycol (PPG) was used as a dispersion medium for the preparation of microspheres (MS) consisting of starch, gelatin, whey protein or dextran. Aqueous solutions of the polymers were dispersed in PPG at various initial temperatures and then the systems were cooled to 0.5 degrees C to allow water in the disp...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Seki T,Shinohara K,Kato N,Uchida M,Natsume H,Morimoto K,Juni K

    更新日期:2007-03-01 00:00:00

  • Binding properties of adenosine deaminase interacted with theophylline.

    abstract::Thermodynamic studies were carried out to evaluate the binding of theophylline on adenosine deaminase (ADA) in 50 mM sodium phosphate buffer pH 7.5, at 300 K, using isothermal titration calorimetry (ITC). A simple method for determination of binding isotherm in the drug--ADA interaction was applied using ITC data. ADA...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Saboury AA,Bagheri S,Ataie G,Amanlou M,Moosavi-Movahedi AA,Hakimelahi GH,Cristalli G,Namaki S

    更新日期:2004-10-01 00:00:00

  • Russuphelin A, a new cytotoxic substance from the mushroom Russula subnigricans Hongo.

    abstract::A new cytotoxic substance, designated russuphelin A (1), has been isolated from the mushroom Russula subnigricans Hongo (Basidiomycetes). The structure was elucidated as 2,6-bis(2,6-dichloro-4-hydroxyphenyloxy)-1,4-dimethoxy-benzene on the basis of spectroscopic data and confirmed by total synthesis. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takahashi A,Agatsuma T,Matsuda M,Ohta T,Nunozawa T,Endo T,Nozoe S

    更新日期:1992-12-01 00:00:00

  • Structures and interaction with DNA of ternary palladium(II) complexes: [Pd(Gly)(X)] (Gly=glycine; X=2,2'-bipyridine, 1,10-phenanthroline and 2,2'-bi-pyridylamine).

    abstract::The structures of the ternary palladium(II) complexes of the formulations [Pd(Gly)(bpy)](+)Cl(-).4H(2)O (Gly=glycine; bpy=2,2'-bipyridine) (1), [Pd(Gly)(phen)](+)Cl(-).4H(2)O (2) (phen=1,10-phenanthroline) and {[Pd(Gly)(bpa)](+)Cl(-)}(2).6H(2)O (3) (bpa=2,2'-bipyridylamine) were determined. All complexes are positivel...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yodoshi M,Okabe N

    更新日期:2008-07-01 00:00:00

  • Lembehsterols A and B, novel sulfated sterols inhibiting thymidine phosphorylase, from the marine sponge Petrosia strongylata.

    abstract::Lembehsterols A (1) and B (2), two novel sulfated sterols, were isolated from the marine sponge Petrosia strongylata. Both sterols showed inhibitory activity against thymidine phosphorylase, which is an enzyme related to angiogenesis in solid tumors. The structures of these sulfated sterols were established on the bas...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Aoki S,Naka Y,Itoh T,Furukawa T,Rachmat R,Akiyama S,Kobayashi M

    更新日期:2002-06-01 00:00:00

  • Studies on differentiation inducers. VI. Lignan derivatives from Arctium fructus. (2).

    abstract::In the previous paper, we reported the differentiation inducing activities of lignoids from Arctium Fructus (the fruits of Arctium lappa L., Compositae) against mouse myeloid leukemia cells (M1). We reinvestigated the active components of this extract and isolated three new dilignans. Furthermore, structure modificati...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Umehara K,Nakamura M,Miyase T,Kuroyanagi M,Ueno A

    更新日期:1996-12-01 00:00:00

  • Studies on the constituents of Ailanthus integrifolia.

    abstract::A new phenolic glycoside, 3,4,5-trimethoxyphenol-1-(6-xylopyranosyl)glucopyranoside, was isolated together with twenty known compounds identified as koaburaside, 3,4,5-trimethoxyphenol, 5,7-dihydroxychromone-7-neohesperidoside, naringin, neoeriocitrin, p-coumaric acid, vanillin, vanillic acid, coniferyl aldehyde, feru...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kosuge K,Mitsunaga K,Koike K,Ohmoto T

    更新日期:1994-08-01 00:00:00

  • Saponins esculeosides B-1 and B-2 in Italian canned tomatoes.

    abstract::Italian canned tomatoes contain the tomato glycosides esculeosides B-1 (1, 0.0052%) and B-2 (2, 0.0068%) without esculeoside A. Herein, the structure of esculeoside B-1 (1) is characterized to be 3-O-β-lycotetraosyl (5S,22R,23S,25S)-22,26-epimino-16β,23-epoxy-3β,23,27-trihydroxycholestane 27-O-β-D-glucopyranoside. We ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Manabe H,Fujiwara Y,Ikeda T,Ono M,Murakami K,Zhou JR,Yokomizo K,Nohara T

    更新日期:2013-01-01 00:00:00

  • Revised structures of gambiriins A1, A2, B1, and B2, chalcane-flavan dimers from gambir (Uncaria gambir extract).

    abstract::Gambir, the aqueous extract from Uncaria gambir (Rubiaceae), has been used as an astringent medicine in Asian countries. Investigation of the constituents in the extract led to the isolation of four chalcane-flavan dimers, gambiriin A1 (6), A2 (7), B1 (8), and B2 (9), in addition to (+)-catechin (1), (+)-epicatechin (...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Taniguchi S,Kuroda K,Doi K,Tanabe M,Shibata T,Yoshida T,Hatano T

    更新日期:2007-02-01 00:00:00

  • Prolonged Distribution of Tranilast in the Eyes after Topical Application onto Eyelid Skin.

    abstract::Tranilast, a lipophilic drug with various ophthalmic applications, was used as a model drug to establish the possibility of delivering lipophilic drugs through the eyelid skin. Pharmacokinetics and tissue distribution studies were conducted employing three application methods (topical application onto eyelid skin, eye...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: See GL,Arce F Jr,Itakura S,Todo H,Sugibayashi K

    更新日期:2020-01-01 00:00:00

  • Particle size effect of metastable calcium phosphates on crushing strength of self-setting bioactive calcium phosphate cement.

    abstract::The resistance to crushing after a self-setting bioactive calcium phosphate cement, consisting of various particle sizes of tetracalcium phosphate (TECP), dicalcium phosphate dihydrate (DCPD) and hydroxyapatite (HAP), had hardened was tested after setting at 37 degrees C, 100% RH. X-ray diffraction suggested that the ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Otsuka M,Matsuda Y,Suwa Y,Fox JL,Higuchi WI

    更新日期:1993-11-01 00:00:00

  • Application of the solid dispersion method to the controlled release of medicine. IV. Precise control of the release rate of a water soluble medicine by using the solid dispersion method applying the difference in the molecular weight of a polymer.

    abstract::Solid dispersions were prepared by the evaporation of ethanol after dissolving into ethanol a water soluble medicine (oxprenolol hydrochloride (OXP)), four grades of water insoluble ethylcellulose (EC) and four grades of water soluble hydroxypropyl cellulose (HPC), both having different molecular weights. The precise ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yuasa H,Ozeki T,Kanaya Y,Oishi K

    更新日期:1993-05-01 00:00:00

  • Solution syntheses of two enkephalin-containing peptides, peptide E and dynorphin(1-24), using Nin-(2,4,6-triisopropylphenylsulfonyl)tryptophan.

    abstract::Two enkephalin-containing peptides, peptide E and dynorphin(1-24), were synthesized by conventional solution methods employing a new Trp derivative, Nin-(2,4,6-triisopropylphenylsulfonyl)tryptophan [Trp(Tps)]. All protecting groups employed including the Tps group were removed by treatment with 1 M trifluoromethanesul...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kitagawa K,Kawamoto T,Futaki S,Kiyama S,Akita T,Moritoki H,Kiso Y

    更新日期:1989-10-01 00:00:00

  • Novel cycloundecapeptides related to gramicidin S with both high antibiotic activity and low hemolytic activity.

    abstract::To find candidates with high antimicrobial and low hemolytic activities, many gramicidin S (GS) analogs of various ring sizes have been designed and synthesized. However, syntheses of antimicrobially active analogues of GS having a disordered symmetry structure from C(2) have almost never been reported, because the st...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Tamaki M,Takanashi K,Harada T,Fujinuma K,Shindo M,Kimura M,Uchida Y

    更新日期:2011-01-01 00:00:00

  • An enkephalin-degrading aminopeptidase from rat brain catalyzes the hydrolysis of a neuropeptide, kyotorphin (L-Tyr-L-Arg).

    abstract::We studied the hydrolysis of a neuropeptide kyotorphin (L-Tyr-L-Arg) by an enkephalin-degrading aminopeptidase purified from cytosol of rat brain in vitro. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), gel filtration and isoelectric focusing. T...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Akasaki K,Tsuji H

    更新日期:1991-07-01 00:00:00

  • Antiulcer activities of glycyrrhetinic acid derivatives in experimental gastric lesion models.

    abstract::Glycyrrhetinic acid (Ia) and eighteen related derivatives were examined for antiulcer activity using stress-induced gastric lesions (restraint plus water immersion at 25 degrees C) in mice and rats as screening tests. Among the compounds tested, dihemiphthalate derivatives of 18 alpha- or 18 beta-olean-12-ene-3 beta,3...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yano S,Harada M,Watanabe K,Nakamaru K,Hatakeyama Y,Shibata S,Takahashi K,Mori T,Hirabayashi K,Takeda M

    更新日期:1989-09-01 00:00:00

  • Three new 15,16-seco-cycloartane glycosides from Cimicifuga Rhizome.

    abstract::Three new 15,16-seco-cycloartane glycosides, which were constructed by a C-C bond cleavage in the D ring, have been isolated from Cimicifuga Rhizome for the first time. Their structures were determined by the use of 2D NMR techniques and chemical evidence. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yoshimitsu H,Nishida M,Nohara T

    更新日期:2007-05-01 00:00:00