Detection of new antigenic proteins in Japanese cedar pollen.


:In Japan, an increasing number of people suffer from pollenosis, a typical atopic disease. Japanese cedar (Cryptomeria japonica) pollen is the most common allergen that causes pollenosis. Although Cry j 1 and Cry j 2 are the common allergenic proteins contained in the pollen, there is a small population of patients who exhibit positive skin reactions to the pollen extract but are negative for both Cry j 1 and Cry j 2. This suggests that the pollen may contain other antigenic proteins besides these two molecules. In this study, we used sodium dodecyl sulfate-polyacrylamide gel electrophoresis to examine partially purified pollen extract (PPPE) and determine if there were other proteins that reacted to anti-PPPE sera collected from sensitized guinea pigs, mice and rabbits. Subsequently, we detected four bands of proteins with molecular weights around 7, 15, 20 and 31 kDa, which were different from those normally seen for Cry j 1 and Cry j 2. Among these, the 7, 15 and 20 kDa proteins could not be detected when anti-Cry j 1 monoclonal anti-body (mAb) and anti-Cry j 2 mAb were used as antibodies for Western blotting. Therefore, these three proteins may differ from Cry j 1 and Cry j 2 not only in molecular weight but also in antigenicity. In conclusion, three new antigenic proteins that are not identical to Cry j 1 and Cry j 2 have been shown to exist in Japanese cedar pollen. Structural analyses of these proteins may be useful in the development of new therapeutic methods, including specific immunotherapy for pollenosis.


Biol Pharm Bull


Matsumura D,Nabe T,Mizutani N,Fujii M,Kohno S




Has Abstract


2006-06-01 00:00:00














  • First-pass metabolism of 5-fluorouracil in the perfused rat small intestine.

    abstract::The first-pass intestinal metabolism of 5-fluorouracil (5-FU) was investigated by single-pass perfusion of the rat small intestine. At the low concentration of 0.06 mg/ml, the fraction of 5-FU absorbed into (i.e., appeared in) the mesenteric venous blood (Fa,b) was about 50% smaller than the fraction absorbed (disappe...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Gu J,Yuasa H,Hayashi Y,Watanabe J

    更新日期:1998-08-01 00:00:00

  • Population Pharmacokinetics in China: The Dynamics of Intravenous Voriconazole in Critically Ill Patients with Pulmonary Disease.

    abstract::Pharmacokinetic research in China on the use of voriconazole in critically ill adult patients with different pulmonary diseases remains to be explored. This study evaluated the population pharmacokinetics of the use of voriconazole (VRC) in critically ill patients to determine covariate effects on VRC pharmacokinetics...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 临床试验,杂志文章


    authors: Chen W,Xie H,Liang F,Meng D,Rui J,Yin X,Zhang T,Xiao X,Cai S,Liu X,Li Y

    更新日期:2015-01-01 00:00:00

  • Effects of isopropyl palmitate on the skin permeation of drugs.

    abstract::The model penetrants oxaprozin, nimesulide, gliclazide, and ribavirin, because of their different lipophilicities, were selected to assess the enhancing activity of pre-treatment solutions consisting of isopropyl palmitate (IP) in ethanol (5%, 10%, 15%and 20%, w/w, respectively) across excised rat skin using Franz dif...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Guo H,Liu Z,Li J,Nie S,Pan W

    更新日期:2006-11-01 00:00:00

  • Effects of Cu, Zn-superoxide dismutase delivered by genetically modified skin fibroblasts on cold-induced skin edema in rats.

    abstract::The effects of Cu, Zn-superoxide disumutase (SOD) delivered by genetically modified skin fibroblasts on cold-induced skin edema were studied in rats. Cold-induced skin edema was induced on the dorsal skin following transplantation of ILSOD cells, genetically modified skin fibroblasts which release secretable SOD prote...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Nishiguchi K,Komada F,Tanigawara Y,Sakai Y,Iwakawa S,Okumura K

    更新日期:1998-12-01 00:00:00

  • Effect of N-B transition on the microenvironment surrounding 34Cys in human serum albumin.

    abstract::The effect of pH on the microenvironment surrounding 34Cys in human serum albumin (HSA) has been studied using acrylodan, a Cys-specific fluorescence probe. The reactivity of 34Cys with 5,5'-dithiobis(2-nitro benzoic acid) (DTNB) followed a pseudo-first-order reaction, and the increase in reactivity was dependent on p...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Narazaki R,Maruyama T,Otagiri M

    更新日期:1997-04-01 00:00:00

  • Structural diversity of mammalian hepatic dermatan sulfates.

    abstract::Dermatan sulfate-proteoglycans (DS-PGs) were extracted from rabbit, rat and bovine defatted livers by magnesium chloride extraction and DEAE-cellulose chromatography, and then submitted successively to Asahipak GS-520 gel filtration chromatography, Asahipak ES-502N anion exchange chromatography, and cellulose acetate ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Koshiishi I,Motoki K,Qiu G,Imanari T

    更新日期:1993-04-01 00:00:00

  • Characterization of the enzymatic and structural properties of human D-aspartate oxidase and comparison with those of the rat and mouse enzymes.

    abstract::D-Aspartate (D-Asp), a free D-amino acid found in mammals, plays crucial roles in the neuroendocrine, endocrine, and central nervous systems. Recent studies have implicated D-Asp in the pathophysiology of infertility and N-methyl-D-Asp receptor-related diseases. D-Asp oxidase (DDO), a degradative enzyme that is stereo...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Katane M,Kawata T,Nakayama K,Saitoh Y,Kaneko Y,Matsuda S,Saitoh Y,Miyamoto T,Sekine M,Homma H

    更新日期:2015-01-01 00:00:00

  • Clinical observation on YiSuiShengXueGranule on treating 156 patients with beta-thalassemia major and the molecular mechanism study.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 临床试验,杂志文章


    authors: Fang S,Wu Z,Zhang X,Liu Y,Wang W,Chai L,Cai H,Yi J,Wang L,Chen Y,Lv X,Huang Y,Wang R,Chen P

    更新日期:2007-11-01 00:00:00

  • Utility decay rates of t(1)-weighted magnetic resonance imaging contrast based on redox-sensitive paramagnetic nitroxyl contrast agents.

    abstract::The availability and applicability of the combination of paramagnetic nitroxyl contrast agent and T(1)-weighted gradient echo (GE)-based dynamic magnetic resonance imaging (MRI) measurement for redox imaging are described. The time courses of T(1)-weighted GE MRI signal intensities according to first-order paramagneti...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsumoto K

    更新日期:2009-04-01 00:00:00

  • Adverse event profile of tigecycline: data mining of the public version of the U.S. Food and Drug Administration adverse event reporting system.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kadoyama K,Sakaeda T,Tamon A,Okuno Y

    更新日期:2012-01-01 00:00:00

  • Species Difference in Sensitivity of Human and Mouse P2X7 Receptors to Inhibitory Effects of Divalent Metal Cations.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Fujiwara M,Ohbori K,Ohishi A,Nishida K,Uozumi Y,Nagasawa K

    更新日期:2017-01-01 00:00:00

  • Effects of alkaloids from Aconitum yesoense var. macroyesoense on cutaneous blood flow in mice.

    abstract::Nine alkaloid constituents in the root of Aconitum yesoense var. macroyesoense, as well as three acetylated derivatives, were examined for their peripheral vaso-activities by measuring laser-flowmetrically the cutaneous blood flow in the hind foot of mice after intravenous administration. The major constitutive delcos...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Wada K,Ishizuki S,Mori T,Bando H,Murayama M,Kawahara N

    更新日期:1997-09-01 00:00:00

  • Molecular identification of "Chuanxiong" by nucleotide sequence and multiplex single base extension analysis on chloroplast trnK gene.

    abstract::Chloroplast trnK gene sequences of Cnidium officinale and Ligusticum chuanxiong were determined to establish an effective method for identifying Japanese Senkyu and Chinese Chuanxiong, the two which have the same drug name in Chinese characters, similar external feature, but different botanical origins. Three sites of...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zhu S,Fushimi H,Han G,Tsuchida T,Uno T,Takano A,Komatsu K

    更新日期:2007-03-01 00:00:00

  • A chromatographic approach for elevating the antibody-dependent cellular cytotoxicity of antibody composites.

    abstract::Recent studies have shown that antibodies with low fucose content in their oligosaccharides exhibit highly potent antibody-dependent cellular cytotoxicity (ADCC). However, composites of therapeutic antibodies produced by conventional production systems using cell lines such as Chinese hamster ovary (CHO) and SP2/0 do ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Tojo S,Okazaki A,Wakitani M,Shinkawa T,Uchida K,Suzawa T

    更新日期:2009-09-01 00:00:00

  • Identification of the substrates for plasma hyaluronan binding protein.

    abstract::Plasma hyaluronan biding protein (PHBP) is a novel serine protease, which has an amino acid sequence homology to that of hepatocyte growth factor activator (HGFA), and has a similar domain structure to that of urinary plasminogen activator (u-PA), found in human plasma. We searched the PHBP substrate in human plasma b...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Choi-Miura NH,Yoda M,Saito K,Takahashi K,Tomita M

    更新日期:2001-02-01 00:00:00

  • Adjuvant effect of grifolan on antibody production in mice.

    abstract::The effects of grifolan (GRN), a gel-forming (1-->6)-branched (1-->3)-beta-D-glucan, on antibody production were examined. Sera from mice that were injected with GRN and trinitrophenyl ovalbumin (TNP-OVA) intraperitoneally showed a significantly increased level of anti-TNP IgG. However, injection of TNP-OVA alone show...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Adachi Y,Suzuki Y,Ohno N,Yadomae T

    更新日期:1998-09-01 00:00:00

  • Up-Regulation of the Voltage-Gated KV2.1 K+ Channel in the Renal Arterial Myocytes of Dahl Salt-Sensitive Hypertensive Rats.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ogiwara K,Ohya S,Suzuki Y,Yamamura H,Imaizumi Y

    更新日期:2017-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ohta Y,Lee JB,Hayashi K,Hayashi T

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


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    更新日期:2002-05-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Jeong GS,Lee DS,Kwon TO,Lee HS,An RB,Kim YC

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Rashid M,Watanabe M,Nakazawa M,Nagatomo T

    更新日期:2001-10-01 00:00:00

  • Nano-mechanical reinforcement in drug-resistant ovarian cancer cells.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Seo YH,Jo YN,Oh YJ,Park S

    更新日期:2015-01-01 00:00:00

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ueki T,Akaishi T,Okumura H,Abe K

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Piao MG,Yang CW,Li DX,Kim JO,Jang KY,Yoo BK,Kim JA,Woo JS,Lyoo WS,Han SS,Lee YB,Kim DD,Yong CS,Choi HG

    更新日期:2008-06-01 00:00:00

  • The distribution of mRNA expression and protein after hydrodynamic injection of transgene in mice.

    abstract::The hydrodynamic method by rapid intravenous injection of a large volume of plasmid DNA is known to be an efficient and liver-specific method of in vivo gene delivery and achieves high levels of foreign gene expression, particularly in hepatocytes. Low transgene activities have also been observed in other organs such ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hattori Y,Koga K,Izumisawa T,Yamasaki M,Narishima R,Yoshida S,Fukui T,Maitani Y

    更新日期:2009-04-01 00:00:00

  • Nucleotide sequence of the gene cluster containing the mphB gene for macrolide 2'-phosphotransferase II.

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    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Katayama J,Noguchi N

    更新日期:1999-02-01 00:00:00

  • Telekin induces apoptosis associated with the mitochondria-mediated pathway in human hepatocellular carcinoma cells.

    abstract::Telekin, a eudesmane-type sesquiterpene lactone compound isolated from Chinese folk medicine Carpesium divaricatum, has been reported to strongly inhibit the proliferation of cancer cells. In this study, the involvement of a mitochondria-mediated pathway in the pro-apoptotic action of telekin was investigated in human...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Zheng B,Wu L,Ma L,Liu S,Li L,Xie W,Li X

    更新日期:2013-01-01 00:00:00

  • Antimicrobial activity of oleanolic acid from Salvia officinalis and related compounds on vancomycin-resistant enterococci (VRE).

    abstract::An extract from Salvia officinalis (Sage) leaves showed antimicrobial activity against vancomycin-resistant enterococci (VRE). We isolated the effective compound and identified it as oleanolic acid, a triterpenoid. We also tested antimicrobial activity of similar triterpenoids, ursolic acid, uvaol, betulinic acid and ...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Horiuchi K,Shiota S,Hatano T,Yoshida T,Kuroda T,Tsuchiya T

    更新日期:2007-06-01 00:00:00

  • Leukotrienes in nociceptive pathway and neuropathic/inflammatory pain.

    abstract::The purpose of this review is to summarize the recent studies examining the expression of leukotrienes (LTs) and their receptors in nociceptive pathways, and their crucial roles in pathological pain conditions. LTs belong to a large family of lipid mediators, termed eicosanoids, which are derived from arachidonic acid...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Noguchi K,Okubo M

    更新日期:2011-01-01 00:00:00

  • Inhibition of itch-scratch response by fruits of Cnidium monnieri in mice.

    abstract::We previously screened the anti-itching activities of 33 herbal medicines in substance P (SP)-induced itching model mice. One of the most potent antipruritogenic extracts, the methanol extract of fruits of Cnidium monnieri (Cnidii Fructus) was studied further. The chloroform-soluble fraction of the methanol extract ma...

    journal_title:Biological & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Basnet P,Yasuda I,Kumagai N,Tohda C,Nojima H,Kuraishi Y,Komatsu K

    更新日期:2001-09-01 00:00:00