Radiation-induced transgenerational alterations in genome stability and DNA damage.


:Mutation induction in directly exposed cells is currently regarded as the main component of the genetic risk of ionizing radiation for humans. However, recent data on the transgenerational increases in mutation rates in the offspring of irradiated parents indicate that the genetic risk could be greater than predicted previously. Here, we have analysed transgenerational changes in mutation rates and DNA damage in the germline and somatic tissues of non-exposed first-generation offspring of irradiated inbred male CBA/Ca and BALB/c mice. Mutation rates at an expanded simple tandem repeat DNA locus and a protein-coding gene (hprt) were significantly elevated in both the germline (sperm) and somatic tissues of all the offspring of irradiated males. The transgenerational changes in mutation rates were attributed to the presence of a persistent subset of endogenous DNA lesions (double- and single-strand breaks), measured by the phosphorylated form of histone H2AX (gamma-H2AX) and alkaline Comet assays. Such remarkable transgenerational destabilization of the F(1) genome may have important implications for cancer aetiology and genetic risk estimates. Our data also provide important clues on the still unknown mechanisms of radiation-induced genomic instability.






Barber RC,Hickenbotham P,Hatch T,Kelly D,Topchiy N,Almeida GM,Jones GD,Johnson GE,Parry JM,Rothkamm K,Dubrova YE




Has Abstract


2006-11-30 00:00:00
















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    abstract::We have previously reported on the identification of a cDNA (placenta growth factor, PlGF) coding for a novel angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). Biochemical and functional characterization of PlGF derived from t...


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    authors: Maglione D,Guerriero V,Viglietto G,Ferraro MG,Aprelikova O,Alitalo K,Del Vecchio S,Lei KJ,Chou JY,Persico MG

    更新日期:1993-04-01 00:00:00

  • The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein.

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    pub_type: 杂志文章


    authors: Bos TJ,Rauscher FJ 3rd,Curran T,Vogt PK

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    authors: Munnes M,Patrone G,Schmitz B,Romeo G,Doerfler W

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    authors: Gupta A,Jha S,Engel DA,Ornelles DA,Dutta A

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    authors: Janssen A,Medema RH

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    authors: Runeberg-Roos P,Virtanen H,Saarma M

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    authors: Pollice A,Sepe M,Villella VR,Tolino F,Vivo M,Calabrò V,La Mantia G

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    pub_type: 杂志文章


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