Characterization of opioid-sensitive neurons in the anteroventral third ventricle region of polydipsic inbred mice in vitro.


:In previous studies we found that in the extremely polydipsic special strain of mice, STR/N, spontaneous drinking was greatly attenuated by injection of the opioid antagonists given intracerebroventricularly as well as subcutaneously. Therefore, we investigated, using hypothalamic slice preparations, responses of neurons in the anteroventral third ventricle region (AV3V) of the STR/N and its control, Swiss/Webster (S/W) mice to morphine and opiate peptides. An application of morphine at 10(-6) M to the circulating medium inhibited activities of 44% of AV3V neurons (45 of 102) in the STR/N, and 59% (76/129) in the S/W, demonstrating that morphine affected a smaller proportion of neurons of the polydipsic mice than that of controls. Opioid agonists for 3 receptor types, mu, delta and kappa, at 10(-6) to 10(-5) M inhibited AV3V neurons in both the STR/N and S/W mice, but to a different degree. No cell of either strain was excited by morphine or any of the opioids. The mu-receptor agonist, [D-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin (DAGO), was the most potent inhibitor of AV3V neurons; in the STR/N 53% (25/47), and in the S/W 77% (34/44) were inhibited. The kappa-agonist, dynorphin A-(1-13) (DYN), inhibited fewer cells in the STR/N (9%, 4/47), compared with the S/W (36%, 16/44). Only a few cells responded to the delta-agonist, [D-Pen2,5]enkephalin (DPDPE), in both strains. The inhibitory actions of the opiates were reversibly blocked by naloxone, and persisted under synaptic blockade. The threshold concentration of morphine or DAGO for inhibition of AV3V neurons was higher in the STR/N (approximately 10(-8) M for both morphine and DAGO) than in S/W mice (approximately 10(-9) M for morphine and less than 10(-9) M for DAGO). Although the AV3V also contains angiotensin II-sensitive neurons, they were not affected by morphine (10(-6) M). Similarly neurons inhibited by morphine were not excited by angiotensin II (10(-7) M); some neurons were unresponsive to both chemicals. We conclude that morphine and opiate peptides directly inhibit the AV3V neurons of both the STR/N and S/W strains of mice and the sensitivity of these neurons to the opiates is lower in the polydipsic inbred mice compared to their controls. The results, together with our behavioral studies, suggest involvement of the central opioid system in the polydipsia of the STR/N mice.


Brain Res


Brain research


Hattori Y,Katafuchi T,Koizumi K




Has Abstract


1991-01-11 00:00:00














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    journal_title:Brain research

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    authors: Hori Y,Lee KH,Chung JM,Endo K,Willis WD

    更新日期:1984-07-30 00:00:00

  • AMPA-induced dark cell degeneration of cerebellar Purkinje neurons involves activation of caspases and apparent mitochondrial dysfunction.

    abstract::Cerebellar Purkinje neurons (PNs) are selectively vulnerable to AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepriopionic acid)-induced delayed neurotoxicity known as dark cell degeneration (DCD) that is expressed as cytoplasmic and nuclear condensation, neuron shrinkage, and failure of physiology. The present study ...

    journal_title:Brain research

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    authors: Strahlendorf J,Box C,Attridge J,Diertien J,Finckbone V,Henne WM,Medina MS,Miles R,Oomman S,Schneider M,Singh H,Veliyaparambil M,Strahlendorf H

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  • Two physiologically distinct populations of neurons in the ventrolateral medulla innervate the locus coeruleus.

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    journal_title:Brain research

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    authors: Ennis M,Aston-Jones G

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  • Amyloid β peptide (25-35) in picomolar concentrations modulates the function of glycine receptors in rat hippocampal pyramidal neurons through interaction with extracellular site(s).

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    pub_type: 杂志文章


    authors: Radosevich PM,Lacy DB,Brown LL,Williams PE,Abumrad NN

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  • Spontaneous hippocampal interictal spikes following local kindling: time-course of change and relation to behavioral seizures.

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    更新日期:1989-01-16 00:00:00

  • Intracellular distribution of monoamine oxidase A in selected regions of rat and monkey brain and spinal cord.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Cohen J,Cheng MF

    更新日期:1981-03-02 00:00:00

  • Modification of the responses of primate spinothalamic neurons to mechanical stimulation by excitatory amino acids and an N-methyl-D-aspartate antagonist.

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Dougherty PM,Willis WD

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Stanley EF

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    journal_title:Brain research

    pub_type: 杂志文章


    authors: Mogenson GJ,Wu M,Tsai CT

    更新日期:1989-04-24 00:00:00