Effect of midazolam on in vitro cerebral endothelial ICAM-1 expression induced by astrocyte-conditioned medium.


BACKGROUND AND OBJECTIVE:Astrocytes exposed to hypoxia produce pro-inflammatory cytokines and upregulate intercellular adhesion molecule-1 on cerebral endothelium. This study investigated the effects of midazolam on this response. METHODS:Mouse astrocytes were exposed to 4 h of hypoxia and 24 h of reoxygenation. Astrocyte-conditioned medium were applied to mouse cerebral endothelial cell cultures for 4 h and 24 h in normoxia. Endothelial cells were pre-incubated for 1 h with midazolam (0, 5, 50 microg L-1). Flow cytometry was used to estimate endothelial ICAM-1 expression. IL-1beta concentrations were measured with ELISA. Repeated comparisons were made using ANOVA and post hoc Tukey Test as appropriate. Data are mean (SD). RESULTS:Mouse cerebral endothelial cell ICAM-1 expression was greater after 24 h exposure to hypoxia-reoxygenation astrocyte-conditioned medium compared to normoxic astrocyte-conditioned medium (mean channel flouresence 112.5 (9.5) vs. 81.5 (7.5), P = 0.01). ICAM-1 expression was decreased by midazolam (5 microg L(-1)) compared to control (mean channel flouresence 81.35 (7.5) vs. 112.5 (9.5), P = 0.01). Supernatant IL-1beta concentrations (pg mL(1)) in astrocytes exposed to hypoxia-reoxygenation were greater than those exposed to normoxia (16.41 (2.35) vs. 10.5 (2.13), P = 0.01). CONCLUSION:We conclude that decreased cerebral endothelial ICAM-1 expression in response to activated glial cell compartment by midazolam may decrease post ischaemic brain inflammation and secondary brain injury.


Eur J Anaesthesiol


Ghori K,Harmon D,Walsh F,Shorten G




Has Abstract


2006-09-01 00:00:00














  • Arterial pressure waveform analysis versus thermodilution cardiac output measurement during open abdominal aortic aneurysm repair: a prospective observational study.

    abstract:BACKGROUND:Arterial pressure waveform analysis enables continuous, minimally invasive measurement of cardiac output. Haemodynamic instability compromises the reliability of the technique and a means of maintaining accurate measurement in this circumstance would be useful. OBJECTIVES:To investigate the accuracy, precis...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Montenij LJ,Buhre WF,de Jong SA,Harms JH,van Herwaarden JA,Kruitwagen CL,de Waal EE

    更新日期:2015-01-01 00:00:00

  • Effect of glucose-insulin-potassium on hyperlactataemia in patients undergoing valvular heart surgery: A randomised controlled study.

    abstract:BACKGROUND:Hyperlactataemia represents oxygen imbalance in the tissues and its occurrence during cardiac surgery is associated with adverse outcomes. Glucose-insulin-potassium (GIK) infusion confers myocardial protection against ischaemia-reperfusion injury and has the potential to reduce lactate release while improvin...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,随机对照试验


    authors: Roh GU,Shim JK,Song JW,Kang HM,Kwak YL

    更新日期:2015-08-01 00:00:00

  • Perioperative care of diabetic patients.

    abstract::Diabetes mellitus is the most common metabolic disease and its incidence is increasing. New classifications have been recently proposed based upon a better knowledge of pathogenesis, mechanisms of glucose transport and insulin resistance. The perioperative care of diabetic patients is less dependent on blood-glucose c...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,评审


    authors: Scherpereel PA,Tavernier B

    更新日期:2001-05-01 00:00:00

  • Nutrition support and treatment of motility disorders in critically ill patients - results of a survey on German intensive care units.

    abstract:BACKGROUND AND OBJECTIVE:To evaluate the current clinical attitude in enteral nutrition support and motility disorders in adult critically ill patients on German intensive care units. METHODS:A total of 1493 questionnaires, including 25 items on the medical environment, treatment of motility disorders and enteral nutr...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Röhm KD,Schöllhorn T,Boldt J,Wolf M,Papsdorf M,Piper SN

    更新日期:2008-01-01 00:00:00

  • Tramadol suppositories are less suitable for post-operative pain relief than rectal acetaminophen/codeine.

    abstract::The suitability of tramadol suppositories for inclusion in our hospital formulary for the treatment of mild to moderate post-operative pain was evaluated. In an open randomized trial, rectal tramadol was compared with our standard treatment acetaminophen/codeine suppositories. We expected tramadol to be equally effect...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Pluim MA,Wegener JT,Rupreht J,Vulto AG

    更新日期:1999-07-01 00:00:00

  • Ultrasonographic finding of lung sliding in patients on mechanical ventilation with alveolar-interstitial syndrome.

    abstract:BACKGROUND AND OBJECTIVES:To determine the visibility of pleural lung sliding in alveolar-interstitial syndrome (AIS) in patients on mechanical ventilation at two different time points, as a confirmatory ultrasonographic method for excluding pneumothorax. METHODS:Fifty-two mechanically ventilated patients in the semir...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Rode B,Vučić M,Siranović M,Kovač N,Kelečić M,Gopčević A

    更新日期:2011-04-01 00:00:00

  • Pulmonary lipiodol embolism during transcatheter arterial chemoembolization for hepatoblastoma under general anaesthesia.

    abstract::We present a case of pulmonary embolism that occurred during the injection of lipiodol during transcatheter arterial chemoembolization under general anaesthesia. A 7-year-old child suffering from a large hepatoblastoma was admitted for arterial chemoembolization and carcinostatic administration. Pulmonary embolism due...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Yamaura K,Higashi M,Akiyoshi K,Itonaga Y,Inoue H,Takahashi S

    更新日期:2000-11-01 00:00:00

  • Gum elastic bougie, capnography and apnoeic oxygenation.

    abstract:BACKGROUND AND AIM:This study assessed the accuracy of using capnography with a modified, hollow gum elastic bougie in predicting tracheal intubation, and its effectiveness as a method of apnoeic oxygenation. METHODS:Patients were randomly allocated to having the gum elastic bougie inserted, under anaesthesia, in the ...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Millar FA,Hutchison GL,Glavin R

    更新日期:2001-01-01 00:00:00

  • Predicted end-tidal sevoflurane concentration for insertion of a Laryngeal Mask Supreme: a prospective observational study.

    abstract:CONTEXT:The single-use Laryngeal Mask Airway (LMA) Supreme is a new supraglottic airway device. It has been reported to be reliable and easy-to-use in clinical practice; however, the anaesthetic techniques for its insertion are not standardised. OBJECTIVES:The purpose of this study was to determine the ED50 of end-tid...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Zaballos M,Bastida E,Jiménez C,Agustí S,López-Gil MT

    更新日期:2013-04-01 00:00:00

  • Propofol as a continuous infusion during cardiopulmonary bypass does not affect changes in serum free fatty acids.

    abstract::Perioperative myocardial infarction or ischaemia is a potential consequence of cardiac surgery and elevated free fatty acids can increase the severity of myocardial ischaemic damage. We investigated perioperative changes in serum free fatty acids, and other serum lipids, as a consequence of using propofol infusions fo...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Inoue S,Takauchi Y,Kayamori Y,Kuro M,Furuya H

    更新日期:2001-02-01 00:00:00

  • Epidural anaesthesia for insertion of a femoral neck prosthesis in a patient with phaeochromocytoma.

    abstract::A hemiarthroplasty for femoral neck fracture was successfully performed under combined epidural anaesthesia and light general anaesthesia before phaeochromocytoma removal. Pre-operative therapy was managed with doxazosin, enalapril and diltiazem. Peri-operative management facilitated maintenance of stable haemodynamic...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Kato T,Takase H,Waguri Y,Ikeda K,Takahashi M

    更新日期:1993-11-01 00:00:00

  • The ProSeal laryngeal mask airway and the laryngeal tube Suction for ventilation in gynaecological patients undergoing laparoscopic surgery.

    abstract:BACKGROUND AND OBJECTIVE:ProSeal Laryngeal Mask Airway (PLMA) and Laryngeal Tube Suction (LTS), supraglottic airway devices allowing gastric drainage, were compared in this prospective, randomized study for airway management under conditions with elevated intra-abdominal pressure induced by capnoperitoneum. METHODS:Fi...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Roth H,Genzwuerker HV,Rothhaas A,Finteis T,Schmeck J

    更新日期:2005-02-01 00:00:00

  • Prevention of atelectasis by continuous positive airway pressure in anaesthetised children: A randomised controlled study.

    abstract:BACKGROUND:Continuous positive airway pressure (CPAP) prevents peri-operative atelectasis in adults, but its effect in children has not been quantified. OBJECTIVE:The aim of this study was to evaluate the role of CPAP in preventing postinduction and postoperative atelectasis in children under general anaesthesia. DES...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Acosta CM,Lopez Vargas MP,Oropel F,Valente L,Ricci L,Natal M,Suarez Sipmann F,Tusman G

    更新日期:2021-01-01 00:00:00

  • The effect of propofol and thiopentone on impairment by reactive oxygen species of endothelium-dependent relaxation in rat aortic rings.

    abstract::The effect in isolated rat aorta of propofol, thiopentone, midazolam, etomidate and fentanyl on the impairment of endothelium-dependent relaxation by reactive oxygen species is reported. Aortic rings were exposed to reactive oxygen species by the electrolysis of the bathing physiological buffer, and endothelium-depend...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Kelicen P,Ismailoglu UB,Erdemli O,Sahin-Erdemli I

    更新日期:1997-05-01 00:00:00

  • Multimodal analgesia with gabapentin, ketamine and dexamethasone in combination with paracetamol and ketorolac after hip arthroplasty: a preliminary study.

    abstract:BACKGROUND AND OBJECTIVE:It has been hypothesized that combinations of analgesics with different mechanisms of action may reduce or even prevent postoperative pain. We, therefore, investigated the analgesic effect of gabapentin, dexamethasone and low-dose ketamine in combination with paracetamol and ketorolac as compar...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,随机对照试验


    authors: Rasmussen ML,Mathiesen O,Dierking G,Christensen BV,Hilsted KL,Larsen TK,Dahl JB

    更新日期:2010-04-01 00:00:00

  • Tracheal intubation with a flexible fibreoptic scope or the McGrath videolaryngoscope in simulated difficult airway scenarios: a randomised controlled manikin study.

    abstract:BACKGROUND:Flexible fibreoptic endoscopic (FFE) intubation is considered the 'gold-standard' when difficult airway management is anticipated. Several videolaryngoscopes have been developed to facilitate intubation by laryngoscopy. OBJECTIVE:The aim of the study was to compare the performance of the McGrath series 5 vi...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,随机对照试验


    authors: Jepsen CH,Gätke MR,Thøgersen B,Mollerup LT,Ruhnau B,Rewers M,Rosenstock CV

    更新日期:2014-03-01 00:00:00

  • Comparison of laryngeal tube with laryngeal mask airway in anaesthetized and paralysed patients.

    abstract:BACKGROUND AND OBJECTIVE:The laryngeal mask has become a widely accepted alternative to endotracheal intubation and mask ventilation. The laryngeal tube is a relatively new supraglottic airway device for airway management. We compared the new version of the laryngeal tube with the laryngeal mask. METHODS:In a randomiz...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,随机对照试验


    authors: Yildiz TS,Solak M,Toker K

    更新日期:2007-07-01 00:00:00

  • A prospective evaluation of the failure rate of spinal anaesthesia for transurethral prostatic resection.

    abstract::The rate of failed spinal anaesthesia, defined as the need for unplanned general anaesthesia, was evaluated in a prospective study involving 137 patients undergoing transurethral resection of the prostate (TURP). The incidence of sensations at the operative site which did not require general anaesthesia was also evalu...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Guinard JP,Carpenter RL,Smith HS

    更新日期:1992-01-01 00:00:00

  • Neurotoxicity with single dose intrathecal midazolam administration.

    abstract:BACKGROUND AND OBJECTIVE:The aim of study was to investigate the electron microscopic changes in the medulla of the spinal cord that occur with intrathecal midazolam administration. METHODS:Twenty-eight albino rabbits of New Zealand type were randomized into two groups. Following anaesthesia, 16 rabbits were given 300...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Ugur B,Basaloglu K,Yurtseven T,Ates U,Aydin ON,Ozenç D,Yurtseven M,Gürel A

    更新日期:2005-12-01 00:00:00

  • High volume and low concentration of anaesthetic solution in the perivascular interscalene sheath determines quality of block and incidence of complications.

    abstract:BACKGROUND AND OBJECTIVE:In the perivascular sheath of the brachial plexus, the volume of anaesthetic solution determines the quality of anaesthetic cover. Fibrous septa may divide the perivascular space into compartments, leading to inadequate diffusion of the anaesthetic solution. The aim of our study was to obtain g...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,随机对照试验


    authors: Pippa P,Cuomo P,Panchetti A,Scarchini M,Poggi G,D'Arienzo M

    更新日期:2006-10-01 00:00:00

  • The neuromuscular effects of 0.6 mg kg(-1) rocuronium in elderly and young adults with or without renal failure.

    abstract:BACKGROUND AND OBJECTIVES:The elderly represent a wide and increasing patient population and significant numbers of elderly patients have chronic renal disease. This study aimed to investigate the neuromuscular effects of 0.6 mg kg(-1) rocuronium under propofol anaesthesia in young adults and elderly patients with or w...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Kocabas S,Yedicocuklu D,Askar FZ

    更新日期:2008-11-01 00:00:00

  • Intravenous ketamine attenuates arterial pressure changes during the induction of anaesthesia with propofol.

    abstract:BACKGROUND AND OBJECTIVE:To investigate whether the administration of ketamine before induction with propofol produces a smaller decrease in arterial pressure. METHODS:Twenty-two patients were assigned to one of two groups to receive either propofol with ketamine (n = 11) or propofol alone (n = 11, control). Anaesthes...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Furuya A,Matsukawa T,Ozaki M,Nishiyama T,Kume M,Kumazawa T

    更新日期:2001-02-01 00:00:00

  • Effects of sequential changes from conventional ventilation to high-frequency oscillatory ventilation at increasing mean airway pressures in an ovine model of combined lung and head injury.

    abstract:BACKGROUND:The objective of this study was to determine the intracranial, cardiovascular and respiratory changes induced by conversion to high-frequency oscillator ventilation from conventional mechanical ventilation at increasing airway pressures. METHODS:In this study, 11 anaesthetized sheep had invasive cardiovascu...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: O'Rourke J,Sheeran P,Heaney M,Talbot R,Geraghty M,Costello J,McDonnell C,Newell J,Mannion D

    更新日期:2007-05-01 00:00:00

  • Neostigmine-based reversal of intermediate acting neuromuscular blocking agents to prevent postoperative residual paralysis: A systematic review.

    abstract:BACKGROUND:Neostigmine is widely used to antagonise residual paralysis. Over the last decades, the benchmark of acceptable neuromuscular recovery has increased progressively to a train-of-four (TOF) ratio of at least 0.9. Raising this benchmark may impact on the efficacy of neostigmine. OBJECTIVE(S):The systematic rev...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Tajaate N,Schreiber JU,Fuchs-Buder T,Jelting Y,Kranke P

    更新日期:2018-03-01 00:00:00

  • Reducing the incidence of sore throat with the laryngeal mask airway.

    abstract::The incidence of sore throat after the use of the laryngeal mask was assessed with a randomized trial in 839 patients. Sore throat occurred more often in women, older patients or after multiple insertion attempts. It was found that the frequency in women, but not in men, was lowered from 11.9% to 3.9% (P = 0.012) by r...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Nott MR,Noble PD,Parmar M

    更新日期:1998-03-01 00:00:00

  • Antiemetic efficacy of metoclopramide and diphenhydramine added to patient-controlled morphine analgesia: a randomised controlled trial.

    abstract:BACKGROUND AND OBJECTIVE:the objective of this study was to assess whether antiemetic drugs metoclopramide and diphenhydramine, administered together as opposed to alone, can have better efficacy in preventing postoperative nausea and vomiting when added to patient-controlled morphine analgesia. PATIENTS AND METHODS:d...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章,随机对照试验


    authors: Lu CW,Jean WH,Wu CC,Shieh JS,Lin TY

    更新日期:2010-12-01 00:00:00

  • Epidural sufentanil for post-operative pain relief: effects of adrenaline.

    abstract::The analgesic, respiratory and haemodynamic effects of epidural sufentanil 75 micrograms (Group 1) or sufentanil 75 micrograms with adrenaline 75 micrograms (Group 2) were studied in 20 patients following abdominal surgery in a double-blind randomized trial. Pain relief, assessed on a linear analogue scale, sedation, ...

    journal_title:European journal of anaesthesiology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Verborgh C,Van der Auwera D,Noorduin H,Camu F

    更新日期:1988-05-01 00:00:00

  • Gradient between dorsalis pedis and radial arterial blood pressures during sevoflurane anaesthesia: A self-control study in patients undergoing neurosurgery.

    abstract:BACKGROUND:The dorsalis pedis artery (DPA) is a good alternative to the radial artery (RA) for invasive blood pressure monitoring when the upper limb is burned or injured, or if the RA is not available. Understanding the pattern of pressure difference between DPA and the commonly used RA during inhalational anaesthesia...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Chen Y,Cui J,Sun JJ,Wang E,Zhu Y,Li Y,Lu K

    更新日期:2016-02-01 00:00:00

  • Biphasic cardiac output changes during onset of spinal anaesthesia in elderly patients.

    abstract:BACKGROUND AND OBJECTIVE:In most studies of cardiac output changes after spinal anaesthesia, the time-resolution is limited. The aim of this study was to demonstrate cardiac output changes with high time-resolution during onset of spinal anaesthesia in elderly patients. METHODS:We investigated 32 patients aged 60 yr s...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Meyhoff CS,Hesselbjerg L,Koscielniak-Nielsen Z,Rasmussen LS

    更新日期:2007-09-01 00:00:00

  • Prediction of fluid responsiveness using lung recruitment manoeuvre in paediatric patients receiving lung-protective ventilation: A prospective observational study.

    abstract:BACKGROUND:Pressure-based dynamic variables are poor predictors of fluid responsiveness in children, and their predictability is expected to reduce further during lung-protective ventilation with a low tidal volume. OBJECTIVE:We hypothesised that lung recruitment manoeuvre (LRM)-induced changes in dynamic variables im...

    journal_title:European journal of anaesthesiology

    pub_type: 杂志文章


    authors: Kim EH,Lee JH,Jang YE,Ji SH,Kim HS,Cho SA,Kim JT

    更新日期:2020-11-11 00:00:00